1319-0113

3-(1H-benzimidazol-2-yl)-8-methoxy-N-[3-(trifluoromethyl)phenyl]chromen-2-imine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1185
Screen concentration 81.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 4672763
SMILES COC1=CC=CC2=C1OC(=NC3=CC=CC(=C3)C(F)(F)F)C(=C2)C4=NC5=CC=CC=C5N4
Standardized SMILES COc1cccc2C=C(C(=Nc3cccc(c3)C(F)(F)F)Oc12)c4nc5ccccc5[nH]4
Molecular weight 435.398
ALogP 6.06
H-bond donor count 1
H-bond acceptor count 7
Response signature DNA damage response

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.38
% growth inhibition (Hom. pool) 1.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4672763
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:-3.92|P-value:4.42E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ILV5(YLR355C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.14||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:LSG1(YGL099W)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.12||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NAT2(YGR147C)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.3||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NMD3(YHR170W)|FD-Score:4.94|P-value:3.94E-7|Clearance:0.42||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRP45(YAL032C)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.14||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:QRI1(YDL103C)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.21||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RNA15(YGL044C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.04||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RRS1(YOR294W)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SYF1(YDR416W)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.19||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:YBR124W(YBR124W_d)|FD-Score:4.53|P-value:2.96E-6|Clearance:0.42||SGD DESC:Putative protein of unknown function Gene:CCT7(YJL111W)|FD-Score:-3.92|P-value:4.42E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ILV5(YLR355C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.14||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:LSG1(YGL099W)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.12||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NAT2(YGR147C)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.3||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NMD3(YHR170W)|FD-Score:4.94|P-value:3.94E-7|Clearance:0.42||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRP45(YAL032C)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.14||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:QRI1(YDL103C)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.21||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RNA15(YGL044C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.04||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RRS1(YOR294W)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SYF1(YDR416W)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.19||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:YBR124W(YBR124W_d)|FD-Score:4.53|P-value:2.96E-6|Clearance:0.42||SGD DESC:Putative protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4672763
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARC1(YGL105W)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNR1(YIL159W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CCW12(YLR110C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:ESBP6(YNL125C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FES1(YBR101C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FRE1(YLR214W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:GAL83(YER027C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCV3(YAL044C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GTT3(YEL017W)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HRD3(YLR207W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:JJJ1(YNL227C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:MAL33(YBR297W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MBP1(YDL056W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MID2(YLR332W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:NOT3(YIL038C)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPL4(YBR170C)|FD-Score:-5.92|P-value:1.61E-9||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PAU23(YLR037C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PET111(YMR257C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PUF3(YLL013C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:QCR6(YFR033C)|FD-Score:5.27|P-value:6.95E-8||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAD55(YDR076W)|FD-Score:8.49|P-value:1.02E-17||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:REE1(YJL217W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:RPL31B(YLR406C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAP155(YFR040W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SKG1(YKR100C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SLT2(YHR030C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF5(YBR289W)|FD-Score:-4.73|P-value:1.10E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNF6(YHL025W)|FD-Score:-6.6|P-value:2.10E-11||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SWS2(YNL081C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TPM1(YNL079C)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRF5(YNL299W)|FD-Score:-3.96|P-value:3.69E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:UTR4(YEL038W)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VAC8(YEL013W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS27(YNR006W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS45(YGL095C)|FD-Score:5.03|P-value:2.47E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR444W(YDR444W_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YFR020W(YFR020W_p)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YML096W(YML096W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YOL114C(YOL114C_p)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR316C-A(YOR316C-A_p)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR195C(YPR195C_d)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ADE8(YDR408C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARC1(YGL105W)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNR1(YIL159W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CCW12(YLR110C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:ESBP6(YNL125C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FES1(YBR101C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FRE1(YLR214W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels Gene:GAL83(YER027C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCV3(YAL044C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GTT3(YEL017W)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HRD3(YLR207W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:JJJ1(YNL227C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:MAL33(YBR297W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MBP1(YDL056W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MID2(YLR332W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:NOT3(YIL038C)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPL4(YBR170C)|FD-Score:-5.92|P-value:1.61E-9||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PAU23(YLR037C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PET111(YMR257C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PUF3(YLL013C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:QCR6(YFR033C)|FD-Score:5.27|P-value:6.95E-8||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAD55(YDR076W)|FD-Score:8.49|P-value:1.02E-17||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:REE1(YJL217W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:RPL31B(YLR406C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAP155(YFR040W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SKG1(YKR100C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SLT2(YHR030C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF5(YBR289W)|FD-Score:-4.73|P-value:1.10E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNF6(YHL025W)|FD-Score:-6.6|P-value:2.10E-11||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SWS2(YNL081C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TPM1(YNL079C)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRF5(YNL299W)|FD-Score:-3.96|P-value:3.69E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:UTR4(YEL038W)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VAC8(YEL013W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS27(YNR006W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS45(YGL095C)|FD-Score:5.03|P-value:2.47E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR444W(YDR444W_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YFR020W(YFR020W_p)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YML096W(YML096W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YOL114C(YOL114C_p)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR316C-A(YOR316C-A_p)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR195C(YPR195C_d)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W4.943.94E-70.42NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YBR124W_d4.532.96E-60.42YBR124W_dPutative protein of unknown function
YGL099W4.112.00E-50.12LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGR147C3.993.35E-50.30NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YGL044C3.681.15E-40.04RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YLR355C3.641.35E-40.14ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YDR416W3.502.29E-40.19SYF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
YDL103C3.314.63E-40.21QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YAL032C3.109.61E-40.14PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YGR047C2.970.001510.02TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YLL018C2.940.001620.03DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YBL035C2.920.001760.01POL12B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
YDL087C2.910.001820.08LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YLR116W2.830.002340.00MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YOR250C2.820.002370.03CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR076W8.491.02E-17RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YFR033C5.276.95E-8QCR6Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
YGL095C5.032.47E-7VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YMR060C4.741.08E-6SAM37Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
YLL013C4.631.85E-6PUF3Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
YNL079C4.602.09E-6TPM1Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
YFR020W_p4.523.10E-6YFR020W_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YGL105W4.356.94E-6ARC1Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress
YJL095W4.308.46E-6BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YER027C3.914.69E-5GAL83One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain
YFR040W3.904.72E-5SAP155Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress
YLR332W3.875.48E-5MID2O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
YKR100C3.701.09E-4SKG1Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell
YNL125C3.671.20E-4ESBP6Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
YDR408C3.671.21E-4ADE8Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway

GO enrichment analysis for SGTC_1185
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1423.71E-28SGTC_558k064-0027 21.3 μMChemDiv (Drug-like library)50885260.205128DNA damage response
0.1343.70E-25SGTC_11861319-0216 26.0 μMChemDiv (Drug-like library)45651360.513514
0.1186.30E-20SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10204160S ribosome export
0.1163.03E-19SGTC_20785212016 200.0 μMChembridge (Fragment library)18997080.036144660S ribosome export
0.1088.25E-17SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.160S ribosome export
0.1081.13E-16SGTC_12092323-2347 50.3 μMChemDiv (Drug-like library)54127600.0918367
0.1071.24E-16SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.38235360S ribosome export
0.1048.92E-16SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.11111160S ribosome export
0.1024.17E-15SGTC_698k064-0052 127.0 μMChemDiv (Drug-like library)50887510.197674DNA damage response
0.1024.97E-15SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.18181860S ribosome export
0.0978.61E-14SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.65714360S ribosome export
0.0979.29E-14SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.12941260S ribosome export
0.0921.29E-12SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.056179860S ribosome export
0.0904.27E-12SGTC_14073909-7909 12.3 μMChemDiv (Drug-like library)44117800.1182860S ribosome export
0.0881.14E-11SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.0851064

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6271391-0720175 μM0.777778997839ChemDiv (Drug-like library)381.426565.60714
SGTC_7661391-0721126 μM0.718751020919ChemDiv (Drug-like library)381.426565.60714
SGTC_6861319-0111510 μM0.6764714562600ChemDiv (Drug-like library)392.4094451560S ribosome export
SGTC_7651319-0117244 μM0.6571433487746ChemDiv (Drug-like library)410.424724.1232560S ribosome export
SGTC_8171391-071546 μM0.5362321353946ChemDiv (Drug-like library)367.399985.12114
SGTC_11861319-021626 μM0.5135144565136ChemDiv (Drug-like library)385.3904435.32615
SGTC_9721319-0219195 μM0.462523903702ChemDiv (Drug-like library)410.424724.12325
SGTC_11981612-100586.7 μM0.4268296998509ChemDiv (Drug-like library)410.424724.37825
SGTC_2803448-36073.04 μM0.383562626179ChemDiv (Drug-like library)293.273543.2940560S ribosome export
SGTC_1958st07651341.9 μM0.382353732122TimTec (Natural product derivative library)262.26283.31360S ribosome export