1486-1332

1-(4-chlorophenyl)-3-[(4-ethoxyphenyl)carbamoyl]-1-(4-methylphenyl)sulfonylurea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1190
Screen concentration 13.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4150046
SMILES CCOC1=CC=C(C=C1)NC(=O)NC(=O)N(C2=CC=C(C=C2)Cl)S(=O)(=O)C3=CC=C(C=C3)C
Standardized SMILES CCOc1ccc(NC(=O)NC(=O)N(c2ccc(Cl)cc2)S(=O)(=O)c3ccc(C)cc3)cc1
Molecular weight 487.9559
ALogP 5.14
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.09
% growth inhibition (Hom. pool) 4.68


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4150046
Download HIP data (tab-delimited text)  (excel)
Gene:ERG8(YMR220W)|FD-Score:3.83|P-value:6.28E-5|Clearance:0.01||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI18(YBR004C)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.09||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HEM13(YDR044W)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HEM4(YOR278W)|FD-Score:3.35|P-value:4.03E-4|Clearance:0.14||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSP10(YOR020C)|FD-Score:3.21|P-value:6.70E-4|Clearance:0.19||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:MAK21(YDR060W)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.1||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:PMI40(YER003C)|FD-Score:5.09|P-value:1.81E-7|Clearance:0.82||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RET1(YOR207C)|FD-Score:-3.31|P-value:4.73E-4|Clearance:0||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPN12(YFR052W)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPS3(YNL178W)|FD-Score:6.77|P-value:6.44E-12|Clearance:1.68||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRN6(YBL014C)|FD-Score:3.74|P-value:9.35E-5|Clearance:0.15||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SPC98(YNL126W)|FD-Score:3.82|P-value:6.63E-5|Clearance:0.09||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFA2(YKR062W)|FD-Score:4.27|P-value:9.74E-6|Clearance:0.44||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TIM12(YBR091C)|FD-Score:-4.13|P-value:1.82E-5|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:ULP1(YPL020C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.05||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:ERG8(YMR220W)|FD-Score:3.83|P-value:6.28E-5|Clearance:0.01||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI18(YBR004C)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.09||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HEM13(YDR044W)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HEM4(YOR278W)|FD-Score:3.35|P-value:4.03E-4|Clearance:0.14||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSP10(YOR020C)|FD-Score:3.21|P-value:6.70E-4|Clearance:0.19||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:MAK21(YDR060W)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.1||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:PMI40(YER003C)|FD-Score:5.09|P-value:1.81E-7|Clearance:0.82||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RET1(YOR207C)|FD-Score:-3.31|P-value:4.73E-4|Clearance:0||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPN12(YFR052W)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPS3(YNL178W)|FD-Score:6.77|P-value:6.44E-12|Clearance:1.68||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRN6(YBL014C)|FD-Score:3.74|P-value:9.35E-5|Clearance:0.15||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SPC98(YNL126W)|FD-Score:3.82|P-value:6.63E-5|Clearance:0.09||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFA2(YKR062W)|FD-Score:4.27|P-value:9.74E-6|Clearance:0.44||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TIM12(YBR091C)|FD-Score:-4.13|P-value:1.82E-5|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:ULP1(YPL020C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.05||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4150046
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADH6(YMR318C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:BAT2(YJR148W)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BLM10(YFL007W)|FD-Score:4.49|P-value:3.51E-6||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:CEX1(YOR112W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLU1(YMR012W)|FD-Score:-4.4|P-value:5.48E-6||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX6(YHR051W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CUS2(YNL286W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CWH43(YCR017C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:END3(YNL084C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FRA2(YGL220W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUN14(YAL008W)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:Mitochondrial protein of unknown function Gene:GCN5(YGR252W)|FD-Score:4.58|P-value:2.34E-6||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GID8(YMR135C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:HMO1(YDR174W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IRC14(YOR135C_d)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISA1(YLL027W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KTR3(YBR205W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:MNT4(YNR059W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRI1(YPR118W)|FD-Score:6.42|P-value:6.69E-11||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MTC6(YHR151C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTD1(YKR080W)|FD-Score:6.81|P-value:4.75E-12||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:OXA1(YER154W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAF1(YBR279W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCA1(YBR295W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDB1(YBR221C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PKR1(YMR123W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMT3(YOR321W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RAD55(YDR076W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD6(YGL058W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RCO1(YMR075W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RMD8(YFR048W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPL27A(YHR010W)|FD-Score:4.84|P-value:6.42E-7||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SEO1(YAL067C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide Gene:SHB17(YKR043C)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHR5(YOL110W)|FD-Score:-4.45|P-value:4.26E-6||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SIM1(YIL123W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SIS2(YKR072C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLX8(YER116C)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SMM1(YNR015W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPS19(YNL202W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SXM1(YDR395W)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TGL5(YOR081C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TGS1(YPL157W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOK1(YJL093C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TPN1(YGL186C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:YBL071C(YBL071C_d)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR113W(YBR113W_d)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YDR239C(YDR239C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGR126W(YGR126W_p)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR139W(YGR139W_d)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIG1(YPL201C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL102C(YIL102C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative protein of unknown function Gene:YJL160C(YJL160C_p)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJL171C(YJL171C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress Gene:YML057C-A(YML057C-A_d)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR196W(YMR196W_p)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR245W(YMR245W_d)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR008C-A(YOR008C-A_p)|FD-Score:-6.14|P-value:4.23E-10||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YPL136W(YPL136W_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:ADE4(YMR300C)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADH6(YMR318C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:BAT2(YJR148W)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BLM10(YFL007W)|FD-Score:4.49|P-value:3.51E-6||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:CEX1(YOR112W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLU1(YMR012W)|FD-Score:-4.4|P-value:5.48E-6||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX6(YHR051W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CUS2(YNL286W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CWH43(YCR017C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:END3(YNL084C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FRA2(YGL220W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUN14(YAL008W)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:Mitochondrial protein of unknown function Gene:GCN5(YGR252W)|FD-Score:4.58|P-value:2.34E-6||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GID8(YMR135C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:HMO1(YDR174W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IRC14(YOR135C_d)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISA1(YLL027W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KTR3(YBR205W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:MNT4(YNR059W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRI1(YPR118W)|FD-Score:6.42|P-value:6.69E-11||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MTC6(YHR151C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTD1(YKR080W)|FD-Score:6.81|P-value:4.75E-12||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:OXA1(YER154W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAF1(YBR279W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCA1(YBR295W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDB1(YBR221C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PKR1(YMR123W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMT3(YOR321W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RAD55(YDR076W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD6(YGL058W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RCO1(YMR075W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RMD8(YFR048W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPL27A(YHR010W)|FD-Score:4.84|P-value:6.42E-7||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SEO1(YAL067C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide Gene:SHB17(YKR043C)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHR5(YOL110W)|FD-Score:-4.45|P-value:4.26E-6||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SIM1(YIL123W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SIS2(YKR072C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLX8(YER116C)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SMM1(YNR015W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPS19(YNL202W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SXM1(YDR395W)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TGL5(YOR081C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TGS1(YPL157W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOK1(YJL093C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TPN1(YGL186C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:YBL071C(YBL071C_d)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR113W(YBR113W_d)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YDR239C(YDR239C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGR126W(YGR126W_p)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR139W(YGR139W_d)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIG1(YPL201C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL102C(YIL102C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative protein of unknown function Gene:YJL160C(YJL160C_p)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJL171C(YJL171C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress Gene:YML057C-A(YML057C-A_d)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR196W(YMR196W_p)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR245W(YMR245W_d)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR008C-A(YOR008C-A_p)|FD-Score:-6.14|P-value:4.23E-10||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YPL136W(YPL136W_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL178W6.776.44E-121.68RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YER003C5.091.81E-70.82PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YKR062W4.279.74E-60.44TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YMR220W3.836.28E-50.01ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YNL126W3.826.63E-50.09SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YBL014C3.749.35E-50.15RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YPL020C3.591.65E-40.05ULP1Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
YDR060W3.542.03E-40.10MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YBR004C3.442.92E-40.09GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YOR278W3.354.03E-40.14HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YOR020C3.216.70E-40.19HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YNL150W_d3.020.001260.08YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YOR116C2.940.001660.00RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YOR362C2.940.001670.01PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YLR166C2.920.001750.03SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR080W6.814.75E-12MTD1NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
YPR118W6.426.69E-11MRI15'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
YHR010W4.846.42E-7RPL27ARibosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
YMR300C4.661.57E-6ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YGR252W4.582.34E-6GCN5Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
YMR075W4.572.44E-6RCO1Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p
YGL220W4.523.11E-6FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YFL007W4.493.51E-6BLM10Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200
YOR135C_d4.434.66E-6IRC14_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
YAL008W4.405.32E-6FUN14Mitochondrial protein of unknown function
YKR043C4.022.94E-5SHB17Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
YMR245W_d3.953.97E-5YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YER090W3.885.12E-5TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YNL084C3.865.70E-5END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YPL157W3.836.36E-5TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs

GO enrichment analysis for SGTC_1190
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0759.72E-9SGTC_1761486-1328 72.8 μMChemDiv (Drug-like library)223102310.6
0.0731.78E-8SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.107143
0.0732.21E-8SGTC_13631636-0063 10.4 μMChemDiv (Drug-like library)60721850.189189Golgi
0.0691.06E-7SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.08DNA intercalators
0.0672.30E-7SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0769231DNA intercalators
0.0664.45E-7SGTC_11283448-9350 235.0 μMChemDiv (Drug-like library)6193860.0631579
0.0655.36E-7SGTC_8580438-0351 2.8 μMChemDiv (Drug-like library)27991190.0864198copper-dependent oxidative stress
0.0641.01E-6SGTC_2695st077686 52.0 μMTimTec (Natural product derivative library)28760920.115789
0.0641.04E-6SGTC_6330437-0093 97.7 μMChemDiv (Drug-like library)28311180.123711DNA intercalators
0.0631.48E-6SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.0675676
0.0621.87E-6SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0649351Golgi
0.0612.39E-6SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.112676
0.0612.95E-6SGTC_512prima-1 270.0 μMICCB bioactive library3229680.0416667DNA intercalators
0.0604.44E-6SGTC_18815652484 16.0 μMMiscellaneous22551290.111111TRP & mitochondrial translation
0.0604.64E-6SGTC_2635hydroquinidine 100.0 μMMicrosource (Natural product library)4429120.09375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2851486-132546 μM0.80392222310230ChemDiv (Drug-like library)457.92995.29624
SGTC_13371486-101140.3 μM0.727273X1337ChemDiv (Drug-like library)443.903314.8142
SGTC_13391486-132122.7 μM0.7272733469382ChemDiv (Drug-like library)443.903324.8124ERG2
SGTC_2051486-133071.9 μM0.6271194118834ChemDiv (Drug-like library)485.983066.26924
SGTC_1941486-132356.9 μM0.6166675178754ChemDiv (Drug-like library)478.348385.47524
SGTC_1761486-132872.83 μM0.622310231ChemDiv (Drug-like library)511.901295.75327
SGTC_5831488-109188.9 μM0.5263164303273ChemDiv (Drug-like library)501.575224.69326
SGTC_1801486-1293102.78 μM0.5230774140536ChemDiv (Drug-like library)491.482815.57427
SGTC_9811488-109454.9 μM0.4782613449522ChemDiv (Drug-like library)489.564523.20327
SGTC_1994063-002441.6 μM0.473684306215ChemDiv (Drug-like library)311.783863.31414