1488-0194

5-bromo-6-[(4,6-dimethylpyrimidin-2-yl)sulfanylmethyl]imidazo[2,1-b][1,3]thiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1194
Screen concentration 225.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1391452
SMILES CC1=CC(=NC(=N1)SCC2=C(N3C=CSC3=N2)Br)C
Standardized SMILES Cc1cc(C)nc(SCc2nc3sccn3c2Br)n1
Molecular weight 355.2765
ALogP 2.93
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.46
% growth inhibition (Hom. pool) 8.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1391452
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.12||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:GLC7(YER133W)|FD-Score:6.03|P-value:8.12E-10|Clearance:1.9||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRR1(YIL026C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.31||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:POP6(YGR030C)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PTI1(YGR156W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPP1(YHR062C)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.27||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP7(YCL031C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SEC21(YNL287W)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.06||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SFH1(YLR321C)|FD-Score:-4.47|P-value:3.83E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:VAS1(YGR094W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.12||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YDL196W(YDL196W_d)|FD-Score:-4.38|P-value:5.98E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:AAR2(YBL074C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.12||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:GLC7(YER133W)|FD-Score:6.03|P-value:8.12E-10|Clearance:1.9||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRR1(YIL026C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.31||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:POP6(YGR030C)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PTI1(YGR156W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPP1(YHR062C)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.27||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP7(YCL031C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SEC21(YNL287W)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.06||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SFH1(YLR321C)|FD-Score:-4.47|P-value:3.83E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:VAS1(YGR094W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.12||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YDL196W(YDL196W_d)|FD-Score:-4.38|P-value:5.98E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1391452
Download HOP data (tab-delimited text)  (excel)
Gene:ABP1(YCR088W)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ACM1(YPL267W)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AHA1(YDR214W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AIM34(YMR003W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:APM4(YOL062C)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARG3(YJL088W)|FD-Score:6.06|P-value:7.01E-10||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO2(YGL148W)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIO2(YGR286C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CHS6(YJL099W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CTA1(YDR256C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CTR9(YOL145C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CWH43(YCR017C)|FD-Score:5.75|P-value:4.55E-9||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYT2(YKL087C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL81(YIR023W)|FD-Score:6.28|P-value:1.64E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DID4(YKL002W)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DMC1(YER179W)|FD-Score:-3.81|P-value:6.83E-5||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DNF1(YER166W)|FD-Score:5.65|P-value:8.07E-9||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DUF1(YOL087C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERG2(YMR202W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FUI1(YBL042C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GLC3(YEL011W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GYP1(YOR070C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAL9(YOL089C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HBS1(YKR084C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HIS1(YER055C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IES6(YEL044W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:ILV1(YER086W)|FD-Score:6.02|P-value:8.53E-10||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:KAP104(YBR017C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LRO1(YNR008W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSB6(YJL100W)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MET18(YIL128W)|FD-Score:-4.86|P-value:5.73E-7||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MMR1(YLR190W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MRI1(YPR118W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:OST5(YGL226C-A)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAU4(YLR461W)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PIB1(YDR313C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIM1(YBL022C)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:RIM20(YOR275C)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RRM3(YHR031C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:RTT107(YHR154W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAS5(YOR213C)|FD-Score:6.2|P-value:2.78E-10||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHU2(YDR078C)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SLT2(YHR030C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF8(YPL002C)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNN1(YNL086W_p)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SPO73(YER046W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SSZ1(YHR064C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:TAT1(YBR069C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TDH1(YJL052W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIP1(YBR067C)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRP2(YER090W)|FD-Score:8.8|P-value:6.65E-19||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:23.4|P-value:7.81E-122||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC7(YNL054W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS1(YKR001C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YCL001W-A(YCL001W-A_p)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR085W(YCR085W_d)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR101C(YCR101C_p)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YGR126W(YGR126W_p)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLF2(YHL014C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL058W(YLL058W_p)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.89|P-value:5.15E-7||SGD DESC:Putative protein of unknown function Gene:YMR155W(YMR155W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR252C(YMR252C_p)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL106W(YOL106W_d)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR277C(YOR277C_d)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPS1(YLR120C)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT35(YHR105W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ABP1(YCR088W)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ACM1(YPL267W)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AHA1(YDR214W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AIM34(YMR003W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:APM4(YOL062C)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARG3(YJL088W)|FD-Score:6.06|P-value:7.01E-10||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO2(YGL148W)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIO2(YGR286C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CHS6(YJL099W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CTA1(YDR256C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CTR9(YOL145C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CWH43(YCR017C)|FD-Score:5.75|P-value:4.55E-9||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYT2(YKL087C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL81(YIR023W)|FD-Score:6.28|P-value:1.64E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DID4(YKL002W)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DMC1(YER179W)|FD-Score:-3.81|P-value:6.83E-5||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DNF1(YER166W)|FD-Score:5.65|P-value:8.07E-9||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DUF1(YOL087C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERG2(YMR202W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FUI1(YBL042C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GLC3(YEL011W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GYP1(YOR070C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAL9(YOL089C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HBS1(YKR084C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HIS1(YER055C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IES6(YEL044W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:ILV1(YER086W)|FD-Score:6.02|P-value:8.53E-10||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:KAP104(YBR017C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LRO1(YNR008W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSB6(YJL100W)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MET18(YIL128W)|FD-Score:-4.86|P-value:5.73E-7||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MMR1(YLR190W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MRI1(YPR118W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:OST5(YGL226C-A)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAU4(YLR461W)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PIB1(YDR313C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIM1(YBL022C)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:RIM20(YOR275C)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RRM3(YHR031C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:RTT107(YHR154W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAS5(YOR213C)|FD-Score:6.2|P-value:2.78E-10||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHU2(YDR078C)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SLT2(YHR030C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF8(YPL002C)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNN1(YNL086W_p)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SPO73(YER046W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SSZ1(YHR064C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:TAT1(YBR069C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TDH1(YJL052W)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIP1(YBR067C)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRP2(YER090W)|FD-Score:8.8|P-value:6.65E-19||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:23.4|P-value:7.81E-122||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC7(YNL054W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS1(YKR001C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YCL001W-A(YCL001W-A_p)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR085W(YCR085W_d)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR101C(YCR101C_p)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YGR126W(YGR126W_p)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLF2(YHL014C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL058W(YLL058W_p)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.89|P-value:5.15E-7||SGD DESC:Putative protein of unknown function Gene:YMR155W(YMR155W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR252C(YMR252C_p)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL106W(YOL106W_d)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR277C(YOR277C_d)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPS1(YLR120C)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT35(YHR105W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W6.038.12E-101.90GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YHR062C4.131.81E-50.27RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR030C3.865.77E-50.17POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR156W3.691.14E-40.00PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YGR094W3.691.14E-40.12VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase
YIL026C3.571.80E-40.31IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YNL287W3.265.55E-40.06SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YBL074C3.206.85E-40.12AAR2Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
YDL060W3.080.001020.16TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YBR060C2.930.001710.12ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YBR002C2.800.002520.01RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YKR086W2.800.002560.10PRP16DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
YPR019W2.700.003490.05MCM4Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
YDR160W2.650.004060.02SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YER172C2.630.004280.19BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C23.407.81E-122TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YER090W8.806.65E-19TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YIR023W6.281.64E-10DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YOR213C6.202.78E-10SAS5Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
YJL088W6.067.01E-10ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YER086W6.028.53E-10ILV1Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
YCR017C5.754.55E-9CWH43Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
YER166W5.658.07E-9DNF1Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
YGL148W5.541.50E-8ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YLR461W5.462.35E-8PAU4Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
YNL086W_p5.423.02E-8SNN1_pPutative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes
YOR070C5.191.05E-7GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YDR078C5.161.23E-7SHU2Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
YMR202W4.983.18E-7ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YMR001C-A_p4.895.15E-7YMR001C-A_pPutative protein of unknown function

GO enrichment analysis for SGTC_1194
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3782.79E-199SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0344828
0.3742.06E-195SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0735294
0.3624.39E-182SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.0701754
0.3395.25E-159SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0675676
0.3372.01E-156SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.0422535
0.3331.33E-152SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0540541
0.3243.43E-144SGTC_11931488-0126 41.5 μMChemDiv (Drug-like library)13847100.305085
0.3221.22E-142SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0675676
0.3061.13E-128SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0441176TSC3-RPN4
0.2984.50E-121SGTC_22967971656 126.5 μMChembridge (Fragment library)6665700.0384615
0.2922.30E-116SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0724638
0.2923.55E-116SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0441176
0.2854.75E-110SGTC_30189082602 49.5 μMChembridge (Drug-like library)173320890.057971
0.2847.92E-110SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.097561
0.2836.21E-109SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0724638

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7021488-019094 μM0.652174949357ChemDiv (Drug-like library)339.273844.1103amide catabolism
SGTC_11921488-009521.4 μM0.363636915875ChemDiv (Drug-like library)333.246124.37902
SGTC_11931488-012641.5 μM0.3050851384710ChemDiv (Drug-like library)354.652664.17303
SGTC_13451488-019231.4 μM0.275862658348ChemDiv (Drug-like library)280.796263.30603
SGTC_10044084-0029199 μM0.262295716894ChemDiv (Drug-like library)323.412083.22414
SGTC_6604031-1742102 μM0.2463776738706ChemDiv (Drug-like library)300.335680.18925redox potentiating
SGTC_13823002-2117298 μM0.2456141497314ChemDiv (Drug-like library)246.35122.7903
SGTC_9332914-0560114 μM0.214286463887ChemDiv (Drug-like library)234.704682.91302
SGTC_1607st00030550.6 μM0.2083335457690TimTec (Natural product derivative library)395.27423.37216
SGTC_2815796887071.43 μM0.1973682977326Chembridge (Drug-like library)387.926184.66914