1502-0147

N-methyl-2-(4-methylphenyl)-3-phenyl-1,2,4-thiadiazol-2-ium-5-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1195
Screen concentration 41.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 817940
SMILES CC1=CC=C(C=C1)[N+]2=C(N=C(S2)NC)C3=CC=CC=C3
Standardized SMILES CNc1nc(c2ccccc2)[n+](s1)c3ccc(C)cc3
Molecular weight 282.3833
ALogP 4.87
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.46
% growth inhibition (Hom. pool) 7.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 817940
Download HIP data (tab-delimited text)  (excel)
Gene:NBP1(YLR457C)|FD-Score:4.35|P-value:6.70E-6|Clearance:0.2||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NSE1(YLR007W)|FD-Score:-3.18|P-value:7.28E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP145(YGL092W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.04||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PRE8(YML092C)|FD-Score:4.81|P-value:7.63E-7|Clearance:0.41||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPC82(YPR190C)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.04||SGD DESC:RNA polymerase III subunit C82 Gene:RSC58(YLR033W)|FD-Score:5.24|P-value:8.08E-8|Clearance:0.41||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.84|P-value:6.56E-7|Clearance:0.41||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.15||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC10(YLR166C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.27||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SFH1(YLR321C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.35||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SNP1(YIL061C)|FD-Score:4.4|P-value:5.44E-6|Clearance:0.05||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPC105(YGL093W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.04||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STH1(YIL126W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.03||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TEL2(YGR099W)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.16||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:NBP1(YLR457C)|FD-Score:4.35|P-value:6.70E-6|Clearance:0.2||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NSE1(YLR007W)|FD-Score:-3.18|P-value:7.28E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP145(YGL092W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.04||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PRE8(YML092C)|FD-Score:4.81|P-value:7.63E-7|Clearance:0.41||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPC82(YPR190C)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.04||SGD DESC:RNA polymerase III subunit C82 Gene:RSC58(YLR033W)|FD-Score:5.24|P-value:8.08E-8|Clearance:0.41||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.84|P-value:6.56E-7|Clearance:0.41||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.15||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC10(YLR166C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.27||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SFH1(YLR321C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.35||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SNP1(YIL061C)|FD-Score:4.4|P-value:5.44E-6|Clearance:0.05||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPC105(YGL093W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.04||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STH1(YIL126W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.03||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TEL2(YGR099W)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.16||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 817940
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ADO1(YJR105W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANT1(YPR128C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APL5(YPL195W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ASC1(YMR116C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:AVO2(YMR068W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BIO3(YNR058W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BRE5(YNR051C)|FD-Score:5.67|P-value:7.05E-9||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CNM67(YNL225C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COX17(YLL009C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CWH41(YGL027C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYB5(YNL111C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ELP4(YPL101W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FET4(YMR319C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMT1(YBL013W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FOX2(YKR009C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GBP2(YCL011C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GIM5(YML094W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HOM6(YJR139C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HST3(YOR025W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:IOC3(YFR013W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC13(YOR235W_d)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC4(YDR540C)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JEN1(YKL217W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:KKQ8(YKL168C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:LTP1(YPR073C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MDM34(YGL219C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MGA1(YGR249W)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MIH1(YMR036C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MNR2(YKL064W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MRI1(YPR118W)|FD-Score:5.83|P-value:2.72E-9||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRPL31(YKL138C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL44(YMR225C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSC7(YHR039C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NMA111(YNL123W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUM1(YDR150W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PAU4(YLR461W)|FD-Score:4.34|P-value:7.05E-6||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PMP3(YDR276C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRO2(YOR323C)|FD-Score:5.45|P-value:2.58E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC1(YDL006W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:QRI5(YLR204W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD1(YPL022W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAM1(YDL090C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RFX1(YLR176C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RPP2A(YOL039W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SCO2(YBR024W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SLC1(YDL052C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SOD1(YJR104C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE3(YPR069C)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SWF1(YDR126W)|FD-Score:6.06|P-value:6.67E-10||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THR1(YHR025W)|FD-Score:17.1|P-value:4.95E-66||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TMA19(YKL056C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TSC3(YBR058C-A)|FD-Score:8.23|P-value:9.52E-17||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUL1(YKL034W)|FD-Score:5.45|P-value:2.48E-8||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBI4(YLL039C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:VPS35(YJL154C)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YBR116C(YBR116C_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR284W(YBR284W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR444W(YDR444W_p)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YEL023C(YEL023C_p)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YGR228W(YGR228W_d)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR125W(YHR125W_d)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL211C(YJL211C_d)|FD-Score:4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR042C(YLR042C_p)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR108C(YLR108C_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YLR345W(YLR345W_p)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR090W(YMR090W_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR315W(YMR315W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR225W(YOR225W_d)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR379C(YOR379C_d)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPL168W(YPL168W_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:ADH4(YGL256W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ADO1(YJR105W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANT1(YPR128C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APL5(YPL195W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ASC1(YMR116C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:AVO2(YMR068W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BIO3(YNR058W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BRE5(YNR051C)|FD-Score:5.67|P-value:7.05E-9||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CNM67(YNL225C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COX17(YLL009C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CWH41(YGL027C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYB5(YNL111C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ELP4(YPL101W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FET4(YMR319C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMT1(YBL013W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FOX2(YKR009C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GBP2(YCL011C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GIM5(YML094W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HOM6(YJR139C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HST3(YOR025W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:IOC3(YFR013W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC13(YOR235W_d)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC4(YDR540C)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JEN1(YKL217W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:KKQ8(YKL168C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:LTP1(YPR073C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MDM34(YGL219C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MGA1(YGR249W)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MIH1(YMR036C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MNR2(YKL064W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MRI1(YPR118W)|FD-Score:5.83|P-value:2.72E-9||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRPL31(YKL138C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL44(YMR225C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSC7(YHR039C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NMA111(YNL123W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUM1(YDR150W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PAU4(YLR461W)|FD-Score:4.34|P-value:7.05E-6||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PMP3(YDR276C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRO2(YOR323C)|FD-Score:5.45|P-value:2.58E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC1(YDL006W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:QRI5(YLR204W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD1(YPL022W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAM1(YDL090C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RFX1(YLR176C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RPP2A(YOL039W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SCO2(YBR024W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SLC1(YDL052C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SOD1(YJR104C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE3(YPR069C)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SWF1(YDR126W)|FD-Score:6.06|P-value:6.67E-10||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THR1(YHR025W)|FD-Score:17.1|P-value:4.95E-66||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TMA19(YKL056C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TSC3(YBR058C-A)|FD-Score:8.23|P-value:9.52E-17||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUL1(YKL034W)|FD-Score:5.45|P-value:2.48E-8||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBI4(YLL039C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:VPS35(YJL154C)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YBR116C(YBR116C_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR284W(YBR284W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR444W(YDR444W_p)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YEL023C(YEL023C_p)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YGR228W(YGR228W_d)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR125W(YHR125W_d)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL211C(YJL211C_d)|FD-Score:4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR042C(YLR042C_p)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR108C(YLR108C_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YLR345W(YLR345W_p)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR090W(YMR090W_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR315W(YMR315W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR225W(YOR225W_d)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR379C(YOR379C_d)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPL168W(YPL168W_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W5.248.08E-80.41RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YCR052W4.846.56E-70.41RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YML092C4.817.63E-70.41PRE8Alpha 2 subunit of the 20S proteasome
YIL061C4.405.44E-60.05SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YLR457C4.356.70E-60.20NBP1Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
YLR321C4.151.67E-50.35SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YLR166C3.807.26E-50.27SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YGL093W3.532.05E-40.04SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YGR099W3.492.40E-40.16TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YGL092W3.334.31E-40.04NUP145Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
YIL126W3.294.95E-40.03STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YFR037C3.265.58E-40.15RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YPR190C3.119.44E-40.04RPC82RNA polymerase III subunit C82
YDR394W3.070.001070.12RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YNL131W2.950.001580.09TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR025W17.104.95E-66THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YBR058C-A8.239.52E-17TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR126W6.066.67E-10SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YPR118W5.832.72E-9MRI15'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
YNR051C5.677.05E-9BRE5Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
YKL034W5.452.48E-8TUL1Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
YOR323C5.452.58E-8PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YPR069C5.131.44E-7SPE3Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
YOR025W5.002.81E-7HST3Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
YGR249W4.741.04E-6MGA1Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
YKR009C4.621.93E-6FOX2Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
YDR540C4.376.08E-6IRC4Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YLR461W4.347.05E-6PAU4Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
YJL211C_d4.347.07E-6YJL211C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2
YDR150W4.261.01E-5NUM1Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex

GO enrichment analysis for SGTC_1195
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2785.05E-105SGTC_370566-0070 1.6 μMChemDiv (Drug-like library)16266860.109091
0.2763.48E-103SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.046875
0.2493.36E-84SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.0980392SWF1 & branched chain AA biosynthesis
0.2467.65E-82SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.0724638
0.2294.80E-71SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.0882353SWF1 & branched chain AA biosynthesis
0.2192.73E-65SGTC_1771327-0068 8.4 μMChemDiv (Drug-like library)153899000.112903
0.2001.90E-54SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0980392
0.1971.28E-52SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.057971TSC3-RPN4
0.1925.42E-50SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0833333TSC3-RPN4
0.1919.67E-50SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.105263TSC3-RPN4
0.1831.22E-45SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.031746TSC3-RPN4
0.1827.98E-45SGTC_2233sulbentine 40.4 μMMiscellaneous676860.103448
0.1801.53E-43SGTC_3710195-0006 7.4 μMChemDiv (Drug-like library)39285930.0925926
0.1751.23E-41SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0655738
0.1742.11E-41SGTC_20435101342 149.0 μMChembridge (Fragment library)6784510.118644SWF1 & branched chain AA biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_13461488-222462.4 μM0.5106383722427ChemDiv (Drug-like library)420.163586.63213
SGTC_9841492-02552.91 μM0.361585198ChemDiv (Drug-like library)344.452726.12813SWF1 & branched chain AA biosynthesis
SGTC_270485-000132.62 μM0.3333332054479ChemDiv (Drug-like library)266.360764.32313RPP1 & pyrimidine depletion
SGTC_8380109-020371.4 μM0.285714759422ChemDiv (Drug-like library)208.258483.75611
SGTC_20084012253172 μM0.255319603940Chembridge (Fragment library)185.225142.05513
SGTC_13521496-099199 μM0.2549023000039ChemDiv (Drug-like library)215.274261.98603tubulin folding & SWR complex
SGTC_1524469-0003551.23 μM0.245283697280ChemDiv (Drug-like library)268.333584.08224
SGTC_6281636-025316 μM0.2452834456143ChemDiv (Drug-like library)331.230245.07213sphingolipid biosynthesis & PDR1
SGTC_10583448-542597.3 μM0.2452837279705ChemDiv (Drug-like library)262.305863.71602
SGTC_13601598-002239.8 μM0.245283761525ChemDiv (Drug-like library)248.322345.07712ERG2