1601-0112

2-hydroxy-N-[(E)-1-(3-nitrophenyl)ethylideneamino]benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1197
Screen concentration 267.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 7350313
SMILES CC(=NNC(=O)C1=CC=CC=C1O)C2=CC(=CC=C2)[N+](=O)[O-]
Standardized SMILES CC(=NNC(=O)c1ccccc1O)c2cccc(c2)[N+](=O)[O-]
Molecular weight 299.2814
ALogP 2.32
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 2.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 7350313
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.01||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CCT3(YJL014W)|FD-Score:3.92|P-value:4.50E-5|Clearance:0.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CFD1(YIL003W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:ERG8(YMR220W)|FD-Score:5.2|P-value:9.78E-8|Clearance:0.26||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LCP5(YER127W)|FD-Score:6.28|P-value:1.67E-10|Clearance:1.08||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:NOP56(YLR197W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.25||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PET9(YBL030C)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.03||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:POP1(YNL221C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.22||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:QNS1(YHR074W)|FD-Score:-3.85|P-value:5.93E-5|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RER2(YBR002C)|FD-Score:3.13|P-value:8.76E-4|Clearance:0.09||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC1(YOR217W)|FD-Score:3.97|P-value:3.54E-5|Clearance:0.06||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RNA15(YGL044C)|FD-Score:4.95|P-value:3.75E-7|Clearance:0.51||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPN12(YFR052W)|FD-Score:-3.93|P-value:4.18E-5|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:YGL069C(YGL069C_d)|FD-Score:3.79|P-value:7.49E-5|Clearance:0.16||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YTH1(YPR107C)|FD-Score:4.44|P-value:4.53E-6|Clearance:0.47||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ABF1(YKL112W)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.01||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CCT3(YJL014W)|FD-Score:3.92|P-value:4.50E-5|Clearance:0.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CFD1(YIL003W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:ERG8(YMR220W)|FD-Score:5.2|P-value:9.78E-8|Clearance:0.26||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LCP5(YER127W)|FD-Score:6.28|P-value:1.67E-10|Clearance:1.08||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:NOP56(YLR197W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.25||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PET9(YBL030C)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.03||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:POP1(YNL221C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.22||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:QNS1(YHR074W)|FD-Score:-3.85|P-value:5.93E-5|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RER2(YBR002C)|FD-Score:3.13|P-value:8.76E-4|Clearance:0.09||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC1(YOR217W)|FD-Score:3.97|P-value:3.54E-5|Clearance:0.06||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RNA15(YGL044C)|FD-Score:4.95|P-value:3.75E-7|Clearance:0.51||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPN12(YFR052W)|FD-Score:-3.93|P-value:4.18E-5|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:YGL069C(YGL069C_d)|FD-Score:3.79|P-value:7.49E-5|Clearance:0.16||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YTH1(YPR107C)|FD-Score:4.44|P-value:4.53E-6|Clearance:0.47||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 7350313
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADH4(YGL256W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AIM4(YBR194W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ARG3(YJL088W)|FD-Score:-5.33|P-value:4.83E-8||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL3(YPL051W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG26(YLR189C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:CAN1(YEL063C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAR1(YPL111W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CLB5(YPR120C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CTK3(YML112W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTP1(YBR291C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:DMC1(YER179W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:ECM14(YHR132C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ELP4(YPL101W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV25(YML012W)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FMP30(YPL103C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FRA2(YGL220W)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GEP5(YLR091W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HIS1(YER055C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IRS4(YKR019C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:LST7(YGR057C)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAP2(YBL091C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MDG1(YNL173C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MFT1(YML062C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MGT1(YDL200C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MHR1(YDR296W)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRP7(YNL005C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL17(YNL252C)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NCL1(YBL024W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NTE1(YML059C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:PAR32(YDL173W)|FD-Score:-3.8|P-value:7.34E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PFK1(YGR240C)|FD-Score:5.68|P-value:6.58E-9||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFK27(YOL136C)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PLB2(YMR006C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PMT3(YOR321W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PPZ1(YML016C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD24(YER173W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RPL34A(YER056C-A)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSM25(YIL093C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTT107(YHR154W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SCP160(YJL080C)|FD-Score:4.32|P-value:7.89E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCW4(YGR279C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SFP1(YLR403W)|FD-Score:7.62|P-value:1.26E-14||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SHM1(YBR263W)|FD-Score:-3.91|P-value:4.59E-5||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SIT4(YDL047W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLX5(YDL013W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SOD1(YJR104C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOV1(YMR066W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Mitochondrial protein of unknown function Gene:STO1(YMR125W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUS1(YBR111W-A)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TLG2(YOL018C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOS4(YLR183C)|FD-Score:4.33|P-value:7.35E-6||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication Gene:VPS35(YJL154C)|FD-Score:-4.8|P-value:7.88E-7||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS68(YOL129W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VPS72(YDR485C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAT2(YER024W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YCR023C(YCR023C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL218W(YDL218W_p)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YER097W(YER097W_d)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YKL169C(YKL169C_d)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR122C(YLR122C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR152C(YLR152C_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR290C(YLR290C_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YLR345W(YLR345W_p)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR010W(YMR010W_p)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR082C(YMR082C_d)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR268C(YOR268C_p)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL080C(YPL080C_d)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL113C(YPL113C_p)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPL168W(YPL168W_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YSP2(YDR326C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:AAC1(YMR056C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADH4(YGL256W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AIM4(YBR194W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ARG3(YJL088W)|FD-Score:-5.33|P-value:4.83E-8||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL3(YPL051W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG26(YLR189C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:CAN1(YEL063C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAR1(YPL111W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CLB5(YPR120C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CTK3(YML112W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTP1(YBR291C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:DMC1(YER179W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:ECM14(YHR132C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ELP4(YPL101W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV25(YML012W)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FMP30(YPL103C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FRA2(YGL220W)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GEP5(YLR091W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HIS1(YER055C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:IRS4(YKR019C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:LST7(YGR057C)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAP2(YBL091C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MDG1(YNL173C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MFT1(YML062C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MGT1(YDL200C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MHR1(YDR296W)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRP7(YNL005C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL17(YNL252C)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NCL1(YBL024W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NTE1(YML059C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:PAR32(YDL173W)|FD-Score:-3.8|P-value:7.34E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PFK1(YGR240C)|FD-Score:5.68|P-value:6.58E-9||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFK27(YOL136C)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PLB2(YMR006C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PMT3(YOR321W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PPZ1(YML016C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD24(YER173W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RPL34A(YER056C-A)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSM25(YIL093C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTT107(YHR154W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SCP160(YJL080C)|FD-Score:4.32|P-value:7.89E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCW4(YGR279C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SFP1(YLR403W)|FD-Score:7.62|P-value:1.26E-14||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SHM1(YBR263W)|FD-Score:-3.91|P-value:4.59E-5||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SIT4(YDL047W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLX5(YDL013W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SOD1(YJR104C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOV1(YMR066W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Mitochondrial protein of unknown function Gene:STO1(YMR125W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUS1(YBR111W-A)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TLG2(YOL018C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOS4(YLR183C)|FD-Score:4.33|P-value:7.35E-6||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication Gene:VPS35(YJL154C)|FD-Score:-4.8|P-value:7.88E-7||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS68(YOL129W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VPS72(YDR485C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAT2(YER024W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YCR023C(YCR023C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL218W(YDL218W_p)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YER097W(YER097W_d)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YKL169C(YKL169C_d)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR122C(YLR122C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR152C(YLR152C_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR290C(YLR290C_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YLR345W(YLR345W_p)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR010W(YMR010W_p)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR082C(YMR082C_d)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR268C(YOR268C_p)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL080C(YPL080C_d)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL113C(YPL113C_p)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPL168W(YPL168W_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YSP2(YDR326C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

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Deletion Strain FD score P-value Clearance Gene Gene Description
YER127W6.281.67E-101.08LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YMR220W5.209.78E-80.26ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YGL044C4.953.75E-70.51RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YPR107C4.444.53E-60.47YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YOR217W3.973.54E-50.06RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YJL014W3.924.50E-50.12CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGL069C_d3.797.49E-50.16YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YNL221C3.631.41E-40.22POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YKL112W3.423.18E-40.01ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YBL030C3.413.27E-40.03PET9Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
YLR197W3.383.64E-40.25NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YBR002C3.138.76E-40.09RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YFL022C3.040.001180.04FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YOR249C3.000.001330.05APC5Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
YJR046W2.950.001590.03TAH11DNA replication licensing factor, required for pre-replication complex assembly

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR403W7.621.26E-14SFP1Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
YGR240C5.686.58E-9PFK1Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YOL136C5.141.38E-7PFK276-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
YKL169C_d4.671.52E-6YKL169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
YMR082C_d4.651.62E-6YMR082C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YML016C4.621.94E-6PPZ1Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
YMR056C4.473.89E-6AAC1Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
YIL054W_p4.415.23E-6YIL054W_pProtein of unknown function; expressed at both mRNA and protein levels
YPL113C_p4.376.19E-6YPL113C_pGlyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene
YNL252C4.337.34E-6MRPL17Mitochondrial ribosomal protein of the large subunit
YLR183C4.337.35E-6TOS4Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication
YJL080C4.327.89E-6SCP160Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
YOR321W4.241.11E-5PMT3Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication
YER173W4.231.15E-5RAD24Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
YML112W4.221.22E-5CTK3Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1197
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0831.32E-10SGTC_11881403-0014 4.6 μMChemDiv (Drug-like library)96148670.115942mitochondrial stress
0.0773.78E-9SGTC_28729044037 26.0 μMChembridge (Drug-like library)64651950.15625
0.0758.62E-9SGTC_2231micatex 6.9 μMMiscellaneous207370.107143
0.0715.35E-8SGTC_5203584-0023 682.0 μMChemDiv (Drug-like library)13407900.109589
0.0681.80E-7SGTC_22147179297 48.3 μMChembridge (Fragment library)7697440.0923077superoxide
0.0648.22E-7SGTC_30139080133 71.4 μMChembridge (Drug-like library)170501930.139241
0.0649.02E-7SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004550.178082
0.0631.11E-6SGTC_7874152-0075 138.0 μMChemDiv (Drug-like library)95665390.0958904
0.0631.48E-6SGTC_23669070441 60.6 μMChembridge (Fragment library)68372350.112903
0.0621.96E-6SGTC_1190121-0043 24.8 μMChemDiv (Drug-like library)10923780.212121mitochondrial stress
0.0603.60E-6SGTC_29879018338 71.4 μMChembridge (Drug-like library)50556820.0875RPP1 & pyrimidine depletion
0.0595.53E-6SGTC_23307979373 200.0 μMChembridge (Fragment library)18630070.098591660S ribosome export
0.0596.83E-6SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.109756mitochondrial stress
0.0587.16E-6SGTC_275trichlorophene 5.7 μMMiscellaneous626160.116667mitochondrial stress
0.0587.73E-6SGTC_521r062-0005 13.8 μMChemDiv (Drug-like library)28320330.102941

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13051181-0477144 μM0.5283026252086ChemDiv (Drug-like library)282.337023.39423
SGTC_7531322-0200289 μM0.4406785403907ChemDiv (Drug-like library)275.216961.85526
SGTC_12111056-003332.3 μM0.4029856741922ChemDiv (Drug-like library)324.290881.04235
SGTC_1731st03596463 μM0.3770496739575TimTec (Natural product derivative library)317.253640.26547
SGTC_6260214-000933 μM0.3684213437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_384k007-01445.16 μM0.3650796824886ChemDiv (Drug-like library)303.743561.91234ERAD & cell cycle
SGTC_6880527-0193473 μM0.3606564007154ChemDiv (Drug-like library)286.24293.10827fatty acid desaturase (OLE1)
SGTC_260302-0163425.16 μM0.3571433100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_3650121-005213.6 μM0.353101739ChemDiv (Drug-like library)361.564684.2624mitochondrial response to ROS
SGTC_22117117706200 μM0.346154822771Chembridge (Fragment library)177.199841.28522