rhodamine 6G

[9-(2-ethoxycarbonylphenyl)-6-(ethylamino)-2,7-dimethylxanthen-3-ylidene]-ethylazanium

Rhodamine 6G is a noncompetitive inhibitor of F0F1-ATPase

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_120
Screen concentration 52.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 65211
SMILES CCNC1=C(C=C2C(=C1)OC3=CC(=[NH+]CC)C(=CC3=C2C4=CC=CC=C4C(=O)OCC)C)C
Standardized SMILES CCNc1cc2OC3=CC(=[NH+]CC)C(=CC3=C(c4ccccc4C(=O)OCC)c2cc1C)C
Molecular weight 443.5573
ALogP 5.47
H-bond donor count 2
H-bond acceptor count 4
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.55
% growth inhibition (Hom. pool) 8.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 65211
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.07||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ARC15(YIL062C)|FD-Score:-4.14|P-value:1.72E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:ATP16(YDL004W)|FD-Score:4.56|P-value:2.52E-6|Clearance:0.05||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CDC53(YDL132W)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.03||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DAD1(YDR016C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP10(YDL031W)|FD-Score:3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DPM1(YPR183W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ECM9(YKR004C)|FD-Score:4.38|P-value:5.99E-6|Clearance:0.27||SGD DESC:Non-essential protein of unknown function Gene:EMG1(YLR186W)|FD-Score:-4.2|P-value:1.34E-5|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GAA1(YLR088W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.07||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GLC7(YER133W)|FD-Score:7.04|P-value:9.61E-13|Clearance:0.84||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.06||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSP60(YLR259C)|FD-Score:6.2|P-value:2.79E-10|Clearance:0.11||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:HYP2(YEL034W)|FD-Score:11.7|P-value:5.54E-32|Clearance:3.36||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:ILV5(YLR355C)|FD-Score:5.07|P-value:2.04E-7|Clearance:0.37||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:KIN28(YDL108W)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.02||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:KRE9(YJL174W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.07||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:MCM2(YBL023C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MIA40(YKL195W)|FD-Score:8.35|P-value:3.44E-17|Clearance:1.31||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAM9(YNL137C)|FD-Score:5.45|P-value:2.52E-8|Clearance:0.22||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NOP8(YOL144W)|FD-Score:5.74|P-value:4.77E-9|Clearance:0.29||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NRD1(YNL251C)|FD-Score:4.01|P-value:3.05E-5|Clearance:0.09||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:NUP192(YJL039C)|FD-Score:3.19|P-value:7.10E-4|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PIS1(YPR113W)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.01||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP5(YAL033W)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RER2(YBR002C)|FD-Score:4.51|P-value:3.24E-6|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPN2(YIL075C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.01||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RSC3(YDR303C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:RSC6(YCR052W)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.23|P-value:8.56E-8|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:3.81|P-value:6.83E-5|Clearance:0.25||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSE1(YML049C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.04||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SAD1(YFR005C)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.01||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SEC21(YNL287W)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPT6(YGR116W)|FD-Score:4.62|P-value:1.92E-6|Clearance:0.06||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SRP1(YNL189W)|FD-Score:-4.56|P-value:2.52E-6|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:STH1(YIL126W)|FD-Score:3.19|P-value:7.18E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TAF10(YDR167W)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.03||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF9(YMR236W)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.1||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TAH11(YJR046W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.04||SGD DESC:DNA replication licensing factor, required for pre-replication complex assembly Gene:TIM10(YHR005C-A)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOA2(YKL058W)|FD-Score:6.1|P-value:5.45E-10|Clearance:0.36||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:UTP15(YMR093W)|FD-Score:4.45|P-value:4.24E-6|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR114C(YGR114C_d)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:3.92|P-value:4.40E-5|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YTH1(YPR107C)|FD-Score:3.82|P-value:6.77E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACP1(YKL192C)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.07||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ARC15(YIL062C)|FD-Score:-4.14|P-value:1.72E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:ATP16(YDL004W)|FD-Score:4.56|P-value:2.52E-6|Clearance:0.05||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CDC53(YDL132W)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.03||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DAD1(YDR016C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP10(YDL031W)|FD-Score:3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DPM1(YPR183W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ECM9(YKR004C)|FD-Score:4.38|P-value:5.99E-6|Clearance:0.27||SGD DESC:Non-essential protein of unknown function Gene:EMG1(YLR186W)|FD-Score:-4.2|P-value:1.34E-5|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GAA1(YLR088W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.07||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GLC7(YER133W)|FD-Score:7.04|P-value:9.61E-13|Clearance:0.84||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.06||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSP60(YLR259C)|FD-Score:6.2|P-value:2.79E-10|Clearance:0.11||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:HYP2(YEL034W)|FD-Score:11.7|P-value:5.54E-32|Clearance:3.36||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:ILV5(YLR355C)|FD-Score:5.07|P-value:2.04E-7|Clearance:0.37||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:KIN28(YDL108W)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.02||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:KRE9(YJL174W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.07||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:MCM2(YBL023C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MIA40(YKL195W)|FD-Score:8.35|P-value:3.44E-17|Clearance:1.31||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAM9(YNL137C)|FD-Score:5.45|P-value:2.52E-8|Clearance:0.22||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NOP8(YOL144W)|FD-Score:5.74|P-value:4.77E-9|Clearance:0.29||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NRD1(YNL251C)|FD-Score:4.01|P-value:3.05E-5|Clearance:0.09||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:NUP192(YJL039C)|FD-Score:3.19|P-value:7.10E-4|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PIS1(YPR113W)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.01||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP5(YAL033W)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RER2(YBR002C)|FD-Score:4.51|P-value:3.24E-6|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPN2(YIL075C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.01||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RSC3(YDR303C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:RSC6(YCR052W)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.23|P-value:8.56E-8|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:3.81|P-value:6.83E-5|Clearance:0.25||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSE1(YML049C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.04||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SAD1(YFR005C)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.01||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SEC21(YNL287W)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPT6(YGR116W)|FD-Score:4.62|P-value:1.92E-6|Clearance:0.06||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SRP1(YNL189W)|FD-Score:-4.56|P-value:2.52E-6|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:STH1(YIL126W)|FD-Score:3.19|P-value:7.18E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TAF10(YDR167W)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.03||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF9(YMR236W)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.1||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TAH11(YJR046W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.04||SGD DESC:DNA replication licensing factor, required for pre-replication complex assembly Gene:TIM10(YHR005C-A)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOA2(YKL058W)|FD-Score:6.1|P-value:5.45E-10|Clearance:0.36||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:UTP15(YMR093W)|FD-Score:4.45|P-value:4.24E-6|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR114C(YGR114C_d)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:3.92|P-value:4.40E-5|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YTH1(YPR107C)|FD-Score:3.82|P-value:6.77E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 65211
Download HOP data (tab-delimited text)  (excel)
Gene:AEP3(YPL005W)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AGA2(YGL032C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:APL6(YGR261C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:CAN1(YEL063C)|FD-Score:12.9|P-value:1.27E-38||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.87|P-value:5.39E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.51|P-value:1.80E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCI1(YOR180C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DIE2(YGR227W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DNF1(YER166W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EDC3(YEL015W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:18.8|P-value:2.95E-79||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FUR4(YBR021W)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCV3(YAL044C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GEP3(YOR205C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GEP4(YHR100C)|FD-Score:7.68|P-value:7.72E-15||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIN4(YDR507C)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GNT1(YOR320C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:HOM6(YJR139C)|FD-Score:12.2|P-value:1.53E-34||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOS4(YIL112W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:HTD2(YHR067W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HUL4(YJR036C)|FD-Score:4.31|P-value:8.09E-6||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IMP2'(YIL154C)|FD-Score:6.99|P-value:1.40E-12||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INM1(YHR046C)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:KAR4(YCL055W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:LCB5(YLR260W)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LYP1(YNL268W)|FD-Score:4.78|P-value:8.56E-7||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MBP1(YDL056W)|FD-Score:5.73|P-value:5.03E-9||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MRH4(YGL064C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP7(YNL005C)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL13(YKR006C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL20(YKR085C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL40(YPL173W)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS12(YNR036C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSA2(YKR077W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSF1(YPR047W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSK1(YNL073W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:OM45(YIL136W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OPT2(YPR194C)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PDC1(YLR044C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PET123(YOR158W)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PIF1(YML061C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIP2(YOR363C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRM2(YIL037C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PRO2(YOR323C)|FD-Score:7.92|P-value:1.16E-15||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS5(YOL061W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:RAD16(YBR114W)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:REC102(YLR329W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RIM1(YCR028C-A)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RML2(YEL050C)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL4A(YBR031W)|FD-Score:4.27|P-value:9.82E-6||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RSM25(YIL093C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAT4(YCR008W)|FD-Score:6.02|P-value:8.60E-10||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SCT1(YBL011W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SDS3(YIL084C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SHE9(YDR393W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SLM5(YCR024C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SNF4(YGL115W)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SOD2(YHR008C)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOV1(YMR066W)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Mitochondrial protein of unknown function Gene:SVF1(YDR346C)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:THI3(YDL080C)|FD-Score:6.05|P-value:7.22E-10||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:THR1(YHR025W)|FD-Score:6.82|P-value:4.45E-12||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIP1(YBR067C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TPN1(YGL186C)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO4(YOR273C)|FD-Score:5.31|P-value:5.54E-8||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRK1(YJL129C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UBA3(YPR066W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBX2(YML013W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:ULS1(YOR191W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VAN1(YML115C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VEL1(YGL258W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS61(YDR136C_d)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VTS1(YOR359W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YDL009C(YDL009C_p)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER053C-A(YER053C-A_p)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER156C(YER156C_p)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR107W(YGR107W_d)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHM2(YMR241W)|FD-Score:4.9|P-value:4.82E-7||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKL027W(YKL027W)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL033W-A(YKL033W-A_p)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YME2(YMR302C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML007C-A(YML007C-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR052C-A(YMR052C-A_d)|FD-Score:-3.95|P-value:3.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR187C(YMR187C_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YMR326C(YMR326C_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YOR200W(YOR200W_d)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPL062W(YPL062W_d)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR071W(YPR071W_p)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR089W(YPR089W_p)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPS3(YLR121C)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:AEP3(YPL005W)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AGA2(YGL032C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:APL6(YGR261C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:CAN1(YEL063C)|FD-Score:12.9|P-value:1.27E-38||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.87|P-value:5.39E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.51|P-value:1.80E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCI1(YOR180C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DIE2(YGR227W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DNF1(YER166W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EDC3(YEL015W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:18.8|P-value:2.95E-79||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FUR4(YBR021W)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCV3(YAL044C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GEP3(YOR205C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GEP4(YHR100C)|FD-Score:7.68|P-value:7.72E-15||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIN4(YDR507C)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GNT1(YOR320C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:HOM6(YJR139C)|FD-Score:12.2|P-value:1.53E-34||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOS4(YIL112W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:HTD2(YHR067W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HUL4(YJR036C)|FD-Score:4.31|P-value:8.09E-6||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IMP2'(YIL154C)|FD-Score:6.99|P-value:1.40E-12||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INM1(YHR046C)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:KAR4(YCL055W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:LCB5(YLR260W)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LYP1(YNL268W)|FD-Score:4.78|P-value:8.56E-7||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MBP1(YDL056W)|FD-Score:5.73|P-value:5.03E-9||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MRH4(YGL064C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP7(YNL005C)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL13(YKR006C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL20(YKR085C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL40(YPL173W)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS12(YNR036C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSA2(YKR077W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSF1(YPR047W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSK1(YNL073W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:OM45(YIL136W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OPT2(YPR194C)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PDC1(YLR044C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PET123(YOR158W)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PIF1(YML061C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIP2(YOR363C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRM2(YIL037C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PRO2(YOR323C)|FD-Score:7.92|P-value:1.16E-15||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS5(YOL061W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:RAD16(YBR114W)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:REC102(YLR329W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RIM1(YCR028C-A)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RML2(YEL050C)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL4A(YBR031W)|FD-Score:4.27|P-value:9.82E-6||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RSM25(YIL093C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAT4(YCR008W)|FD-Score:6.02|P-value:8.60E-10||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SCT1(YBL011W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SDS3(YIL084C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SHE9(YDR393W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SLM5(YCR024C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SNF4(YGL115W)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SOD2(YHR008C)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOV1(YMR066W)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Mitochondrial protein of unknown function Gene:SVF1(YDR346C)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:THI3(YDL080C)|FD-Score:6.05|P-value:7.22E-10||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:THR1(YHR025W)|FD-Score:6.82|P-value:4.45E-12||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIP1(YBR067C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TPN1(YGL186C)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO4(YOR273C)|FD-Score:5.31|P-value:5.54E-8||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRK1(YJL129C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UBA3(YPR066W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBX2(YML013W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:ULS1(YOR191W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VAN1(YML115C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VEL1(YGL258W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS61(YDR136C_d)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VTS1(YOR359W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YDL009C(YDL009C_p)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YER053C-A(YER053C-A_p)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER156C(YER156C_p)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR107W(YGR107W_d)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHM2(YMR241W)|FD-Score:4.9|P-value:4.82E-7||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKL027W(YKL027W)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKL033W-A(YKL033W-A_p)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YME2(YMR302C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML007C-A(YML007C-A_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR052C-A(YMR052C-A_d)|FD-Score:-3.95|P-value:3.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR187C(YMR187C_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YMR326C(YMR326C_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YOR200W(YOR200W_d)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPL062W(YPL062W_d)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR071W(YPR071W_p)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR089W(YPR089W_p)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPS3(YLR121C)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YEL034W11.705.54E-323.36HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YKL195W8.353.44E-171.31MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YER133W7.049.61E-130.84GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YLR259C6.202.79E-100.11HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YKL058W6.105.45E-100.36TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YOL144W5.744.77E-90.29NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YNL137C5.452.52E-80.22NAM9Mitochondrial ribosomal component of the small subunit
YFR037C5.238.56E-80.16RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YLR355C5.072.04E-70.37ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YKL192C4.691.35E-60.07ACP1Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
YGR116W4.621.92E-60.06SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YDL004W4.562.52E-60.05ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YBR002C4.513.24E-60.06RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YMR093W4.454.24E-60.07UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YKR004C4.385.99E-60.27ECM9Non-essential protein of unknown function

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL012W18.802.95E-79ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YEL063C12.901.27E-38CAN1Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
YJR139C12.201.53E-34HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YOR323C7.921.16E-15PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YHR100C7.687.72E-15GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YIL154C6.991.40E-12IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YHR025W6.824.45E-12THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YDL080C6.057.22E-10THI3Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis
YCR008W6.028.60E-10SAT4Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
YDL056W5.735.03E-9MBP1Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
YOR158W5.462.35E-8PET123Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
YOR273C5.315.54E-8TPO4Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
YGL115W5.081.87E-7SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YMR241W4.904.82E-7YHM2Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
YNL268W4.788.56E-7LYP1Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids

GO enrichment analysis for SGTC_120
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2582.36E-90SGTC_13712421-0008 32.7 μMChemDiv (Drug-like library)28275520.0989011mitochondrial processes
0.2061.89E-57SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)54979110.0909091calcium & mitochondrial duress
0.1942.53E-51SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.0989011mitochondrial processes
0.1763.19E-42SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.0990991calcium & mitochondrial duress
0.1681.56E-38SGTC_467ro 31-8220 72.8 μMICCB bioactive library50830.11calcium & mitochondrial duress
0.1648.72E-37SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.0776699heme biosynthesis & mitochondrial translocase
0.1603.53E-35SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.0550459calcium & mitochondrial duress
0.1532.76E-32SGTC_1954234-0266 16.7 μMChemDiv (Drug-like library)23033540.105882
0.1505.46E-31SGTC_630099-0242 26.7 μMChemDiv (Drug-like library)38985020.0481928mitochondrial stress
0.1491.13E-30SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.106667
0.1477.70E-30SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0736842ERG2
0.1424.17E-28SGTC_23447990960 77.0 μMChembridge (Fragment library)1408130.106667
0.1411.80E-27SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.137931mitochondrial processes
0.1402.86E-27SGTC_400oxethazaine 37.5 μMMiscellaneous46210.114943calcium & mitochondrial duress
0.1381.89E-26SGTC_2566crystal violet carbinol 226.0 nMMiscellaneous680500.0526316

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1871st05744867.3 μM0.253165691747TimTec (Natural product derivative library)297.101462.81204
SGTC_3008907891271.43 μM0.2516648731Chembridge (Drug-like library)287.310482.21314
SGTC_1917st06650728.5 μM0.2446813156428TimTec (Natural product derivative library)399.3443063.33218Golgi
SGTC_3047909212249.47 μM0.23809517173831Chembridge (Drug-like library)274.271961.84715
SGTC_1904st06022578 μM0.23684266595TimTec (Natural product derivative library)204.221882.67803
SGTC_22137173844200 μM0.234568892728Chembridge (Fragment library)259.300382.62204
SGTC_14944358-1428177 μM0.2333331564571ChemDiv (Drug-like library)336.813243.11115
SGTC_2626ethyl orsellinate100 μM0.23287775653Microsource (Natural product library)196.199882.03624
SGTC_800159-0065512.24 μM0.2307691548079ChemDiv (Drug-like library)390.431784.49814
SGTC_530973-002339.98 μM0.227848674768ChemDiv (Drug-like library)261.33943.3714RNA processing & uracil transport