1988-0068

1-(5-methyl-2,3-diphenylindol-1-yl)-3-morpholin-4-ylpropan-2-ol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1202
Screen concentration 29.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 2850480
SMILES CC1=CC2=C(C=C1)N(C(=C2C3=CC=CC=C3)C4=CC=CC=C4)CC(CN5CCOCC5)O
Standardized SMILES Cc1ccc2c(c1)c(c3ccccc3)c(c4ccccc4)n2CC(O)CN5CCOCC5
Molecular weight 426.55
ALogP 5.46
H-bond donor count 1
H-bond acceptor count 3
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.51
% growth inhibition (Hom. pool) 8.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2850480
Download HIP data (tab-delimited text)  (excel)
Gene:DBP6(YNR038W)|FD-Score:3.19|P-value:7.22E-4|Clearance:0.14||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:DPB11(YJL090C)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DYS1(YHR068W)|FD-Score:4.5|P-value:3.36E-6|Clearance:0.54||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:GLC7(YER133W)|FD-Score:6.64|P-value:1.60E-11|Clearance:0.76||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KAP95(YLR347C)|FD-Score:5.43|P-value:2.81E-8|Clearance:0.16||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KOG1(YHR186C)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.4||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MRD1(YPR112C)|FD-Score:5.12|P-value:1.51E-7|Clearance:0.4||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NEO1(YIL048W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.07||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PCF11(YDR228C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.22||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PKC1(YBL105C)|FD-Score:3.34|P-value:4.26E-4|Clearance:0.04||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RBA50(YDR527W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.15||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPL15A(YLR029C)|FD-Score:6.19|P-value:3.03E-10|Clearance:0.76||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPT5(YOR117W)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SEC61(YLR378C)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.11||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:TRS130(YMR218C)|FD-Score:5.27|P-value:6.72E-8|Clearance:0.15||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:DBP6(YNR038W)|FD-Score:3.19|P-value:7.22E-4|Clearance:0.14||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:DPB11(YJL090C)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DYS1(YHR068W)|FD-Score:4.5|P-value:3.36E-6|Clearance:0.54||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:GLC7(YER133W)|FD-Score:6.64|P-value:1.60E-11|Clearance:0.76||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KAP95(YLR347C)|FD-Score:5.43|P-value:2.81E-8|Clearance:0.16||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KOG1(YHR186C)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.4||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MRD1(YPR112C)|FD-Score:5.12|P-value:1.51E-7|Clearance:0.4||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NEO1(YIL048W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.07||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PCF11(YDR228C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.22||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PKC1(YBL105C)|FD-Score:3.34|P-value:4.26E-4|Clearance:0.04||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RBA50(YDR527W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.15||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPL15A(YLR029C)|FD-Score:6.19|P-value:3.03E-10|Clearance:0.76||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPT5(YOR117W)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SEC61(YLR378C)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.11||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:TRS130(YMR218C)|FD-Score:5.27|P-value:6.72E-8|Clearance:0.15||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2850480
Download HOP data (tab-delimited text)  (excel)
Gene:AGA2(YGL032C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AQY2(YLL052C)|FD-Score:-4.83|P-value:6.99E-7||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARG3(YJL088W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL1(YBR164C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:BST1(YFL025C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAT8(YMR280C)|FD-Score:4.79|P-value:8.37E-7||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CHO1(YER026C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CLU1(YMR012W)|FD-Score:-6.19|P-value:3.09E-10||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX10(YPL172C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CWH43(YCR017C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYB5(YNL111C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL7(YIR031C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAN1(YJR150C)|FD-Score:4.78|P-value:8.93E-7||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DMC1(YER179W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DSF2(YBR007C_p)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSS1(YMR287C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM30(YLR436C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ECM31(YBR176W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EMC1(YCL045C)|FD-Score:5.67|P-value:7.30E-9||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FUR4(YBR021W)|FD-Score:9.34|P-value:4.79E-21||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:HHY1(YEL059W_d)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:IMP2'(YIL154C)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP54(YOL065C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:JNM1(YMR294W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:MAC1(YMR021C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAM1(YER106W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MAM3(YOL060C)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MIH1(YMR036C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MRM2(YGL136C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MSI1(YBR195C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MTC3(YGL226W)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NIT1(YIL164C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:OPI9(YLR338W_d)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAA1(YDR071C)|FD-Score:-3.72|P-value:9.77E-5||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PDR1(YGL013C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEA2(YER149C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PIB1(YDR313C)|FD-Score:3.75|P-value:8.67E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIM1(YBL022C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN4(YBL051C)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PMS1(YNL082W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:QRI5(YLR204W)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REC104(YHR157W)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RIM15(YFL033C)|FD-Score:-3.74|P-value:9.04E-5||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPL20A(YMR242C)|FD-Score:5.89|P-value:1.92E-9||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAS5(YOR213C)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCP160(YJL080C)|FD-Score:4.58|P-value:2.27E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SGO1(YOR073W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHU2(YDR078C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNF11(YDR073W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SOL3(YHR163W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPE3(YPR069C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SRY1(YKL218C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSD1(YDR293C)|FD-Score:8.69|P-value:1.82E-18||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWD1(YAR003W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:TDH1(YJL052W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIP41(YPR040W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:6.91|P-value:2.38E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TYW3(YGL050W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBI4(YLL039C)|FD-Score:-5.21|P-value:9.62E-8||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBP13(YBL067C)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:6.26|P-value:1.91E-10||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR062C(YBR062C_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR087W(YCR087W_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL242W(YDL242W_d)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGR064W(YGR064W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YKR073C(YKR073C_d)|FD-Score:6.69|P-value:1.11E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Putative protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YPK1(YKL126W)|FD-Score:12.5|P-value:2.08E-36||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR071W(YPR071W_p)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR117W(YPR117W_p)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Putative protein of unknown function Gene:ZPS1(YOL154W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH Gene:AGA2(YGL032C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AQY2(YLL052C)|FD-Score:-4.83|P-value:6.99E-7||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARG3(YJL088W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL1(YBR164C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:BST1(YFL025C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAT8(YMR280C)|FD-Score:4.79|P-value:8.37E-7||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CHO1(YER026C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CLU1(YMR012W)|FD-Score:-6.19|P-value:3.09E-10||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX10(YPL172C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CWH43(YCR017C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYB5(YNL111C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL7(YIR031C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAN1(YJR150C)|FD-Score:4.78|P-value:8.93E-7||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DMC1(YER179W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DSF2(YBR007C_p)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSS1(YMR287C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM30(YLR436C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ECM31(YBR176W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EMC1(YCL045C)|FD-Score:5.67|P-value:7.30E-9||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FUR4(YBR021W)|FD-Score:9.34|P-value:4.79E-21||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:HHY1(YEL059W_d)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:IMP2'(YIL154C)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP54(YOL065C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:JNM1(YMR294W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:MAC1(YMR021C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAM1(YER106W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MAM3(YOL060C)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MIH1(YMR036C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MRM2(YGL136C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MSI1(YBR195C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MTC3(YGL226W)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NIT1(YIL164C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:OPI9(YLR338W_d)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAA1(YDR071C)|FD-Score:-3.72|P-value:9.77E-5||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PDR1(YGL013C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEA2(YER149C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PIB1(YDR313C)|FD-Score:3.75|P-value:8.67E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIM1(YBL022C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN4(YBL051C)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PMS1(YNL082W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:QRI5(YLR204W)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REC104(YHR157W)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RIM15(YFL033C)|FD-Score:-3.74|P-value:9.04E-5||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPL20A(YMR242C)|FD-Score:5.89|P-value:1.92E-9||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAS5(YOR213C)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCP160(YJL080C)|FD-Score:4.58|P-value:2.27E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SGO1(YOR073W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHU2(YDR078C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNF11(YDR073W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SOL3(YHR163W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPE3(YPR069C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SRY1(YKL218C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSD1(YDR293C)|FD-Score:8.69|P-value:1.82E-18||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWD1(YAR003W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:TDH1(YJL052W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIP41(YPR040W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:6.91|P-value:2.38E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TYW3(YGL050W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBI4(YLL039C)|FD-Score:-5.21|P-value:9.62E-8||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBP13(YBL067C)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:6.26|P-value:1.91E-10||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR062C(YBR062C_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR087W(YCR087W_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL242W(YDL242W_d)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGR064W(YGR064W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YKR073C(YKR073C_d)|FD-Score:6.69|P-value:1.11E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Putative protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YPK1(YKL126W)|FD-Score:12.5|P-value:2.08E-36||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR071W(YPR071W_p)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:YPR117W(YPR117W_p)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Putative protein of unknown function Gene:ZPS1(YOL154W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W6.641.60E-110.76GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YLR029C6.193.03E-100.76RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YLR347C5.432.81E-80.16KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YMR218C5.276.72E-80.15TRS130One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
YPR112C5.121.51E-70.40MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YDR228C4.721.17E-60.22PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YHR068W4.503.36E-60.54DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YIL048W3.963.73E-50.07NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YHR186C3.894.98E-50.40KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YDR527W3.492.42E-40.15RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YBL105C3.344.26E-40.04PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YLR378C3.304.88E-40.11SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YNR038W3.197.22E-40.14DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YHR197W3.040.001180.07RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YBR140C2.980.001460.00IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W12.502.08E-36YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YBR021W9.344.79E-21FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YDR293C8.691.82E-18SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YKL211C6.912.38E-12TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YKR073C_d6.691.11E-11YKR073C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR001C6.261.91E-10VPS1Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
YMR242C5.891.92E-9RPL20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
YCL045C5.677.30E-9EMC1Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090
YMR280C4.798.37E-7CAT8Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
YJR150C4.788.93E-7DAN1Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
YJL080C4.582.27E-6SCP160Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
YGL226W4.532.94E-6MTC3Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
YHR163W4.532.98E-6SOL36-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
YIR031C4.473.94E-6DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YMR001C-A_p4.434.63E-6YMR001C-A_pPutative protein of unknown function

GO enrichment analysis for SGTC_1202
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2203.91E-65SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.115789
0.1858.71E-47SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.119565
0.1782.36E-43SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.136364
0.1661.31E-37SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.0864198
0.1641.32E-36SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.1PDR1
0.1624.71E-36SGTC_400oxethazaine 37.5 μMMiscellaneous46210.116883calcium & mitochondrial duress
0.1629.19E-36SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.108108RNA processing & uracil transport
0.1621.01E-35SGTC_1067clotrimazole 1.4 μMNIH Clinical Collection28120.0933333azole & statin
0.1603.04E-35SGTC_813k007-0158 173.0 μMChemDiv (Drug-like library)65239160.0897436fatty acid desaturase (OLE1)
0.1603.43E-35SGTC_1778st046819 58.9 μMTimTec (Natural product derivative library)6717150.0769231
0.1605.60E-35SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0714286
0.1561.58E-33SGTC_2778tioconazole 65.1 nMMiscellaneous54820.0888889ergosterol depletion effects on membrane
0.1561.57E-33SGTC_2620irigenin 100.0 μMMicrosource (Natural product library)54641700.097561
0.1512.18E-31SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.121212calcium & mitochondrial duress
0.1503.15E-31SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.151899

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_10084106-00376 μM0.4328362905394ChemDiv (Drug-like library)443.580525.23214PDR1
SGTC_9001112-0045141 μM0.43103456211ChemDiv (Drug-like library)322.443944.5312NEO1
SGTC_1721112-00462.64 μM0.4032262802135ChemDiv (Drug-like library)391.334065.85912NEO1
SGTC_8880916-00026.97 μM0.4032262802138ChemDiv (Drug-like library)377.307485.40312NEO1
SGTC_8870885-00079.62 μM0.3939395719458ChemDiv (Drug-like library)425.565245.16513endomembrane recycling
SGTC_14414106-004013 μM0.3787882904180ChemDiv (Drug-like library)433.973025.36213
SGTC_10094106-00579.87 μM0.3714292928005ChemDiv (Drug-like library)443.580525.0314
SGTC_14801070-004441.2 μM0.3442623124987ChemDiv (Drug-like library)294.390783.41702
SGTC_3319913845016.76 μM0.3333332029690Chembridge (Drug-like library)379.498563.4915
SGTC_3334914281221.92 μM0.32857145283678Chembridge (Drug-like library)385.968444.31224