2323-2347

3-naphthalen-2-yl-N-[(Z)-thiophen-2-ylmethylideneamino]-1H-pyrazole-5-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1208
Screen concentration 50.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 5412760
SMILES C1=CC=C2C=C(C=CC2=C1)C3=NNC(=C3)C(=O)NN=CC4=CC=CS4
Standardized SMILES O=C(NN=Cc1cccs1)c2cc([nH]n2)c3ccc4ccccc4c3
Molecular weight 346.4057
ALogP 4.23
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.53
% growth inhibition (Hom. pool) 9.38


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5412760
Download HIP data (tab-delimited text)  (excel)
Gene:CDC12(YHR107C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.22||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:FAP7(YDL166C)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:HEM4(YOR278W)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:NTF2(YER009W)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.22||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:PMI40(YER003C)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRE8(YML092C)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PTI1(YGR156W)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:SEC8(YPR055W)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SPC34(YKR037C)|FD-Score:-4.32|P-value:7.68E-6|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:CDC12(YHR107C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.22||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:FAP7(YDL166C)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:HEM4(YOR278W)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:NTF2(YER009W)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.22||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:PMI40(YER003C)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRE8(YML092C)|FD-Score:-4.11|P-value:1.99E-5|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PTI1(YGR156W)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:SEC8(YPR055W)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SPC34(YKR037C)|FD-Score:-4.32|P-value:7.68E-6|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5412760
Download HOP data (tab-delimited text)  (excel)
Gene:ALD4(YOR374W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:BUD32(YGR262C)|FD-Score:-5.19|P-value:1.06E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CCS1(YMR038C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTP1(YBR291C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CWH43(YCR017C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DOS2(YDR068W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:DPP1(YDR284C)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:FCY22(YER060W-A)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FRE8(YLR047C)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FUR4(YBR021W)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN4(YEL009C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDH3(YAL062W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:HIR2(YOR038C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:ILV1(YER086W)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ITR2(YOL103W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:MDM34(YGL219C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MMT1(YMR177W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MRM2(YGL136C)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:NCS2(YNL119W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NPR3(YHL023C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PGD1(YGL025C)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMT3(YOR321W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RFX1(YLR176C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIF1(YBR275C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RNH201(YNL072W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:SGO1(YOR073W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SLX5(YDL013W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNQ2(YDR011W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUR4(YLR372W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAE1(YBR261C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TMA23(YMR269W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TOK1(YJL093C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TRP2(YER090W)|FD-Score:-5.13|P-value:1.48E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUB3(YML124C)|FD-Score:-3.93|P-value:4.22E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS35(YJL154C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS4(YPR173C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VTC3(YPL019C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:VTS1(YOR359W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YGL176C(YGL176C_p)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR139W(YGR139W_d)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR180W(YHR180W_d)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR146W(YJR146W_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YKR073C(YKR073C_d)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL059C(YLL059C_d)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR097C(YOR097C_p)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR131C(YOR131C_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YSC84(YHR016C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ALD4(YOR374W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:BUD32(YGR262C)|FD-Score:-5.19|P-value:1.06E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CCS1(YMR038C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTP1(YBR291C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CWH43(YCR017C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DOS2(YDR068W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:DPP1(YDR284C)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:FCY22(YER060W-A)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FRE8(YLR047C)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FUR4(YBR021W)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN4(YEL009C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDH3(YAL062W)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:HIR2(YOR038C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:ILV1(YER086W)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ITR2(YOL103W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:MDM34(YGL219C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MMT1(YMR177W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MRM2(YGL136C)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:NCS2(YNL119W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NPR3(YHL023C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PGD1(YGL025C)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMT3(YOR321W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RFX1(YLR176C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIF1(YBR275C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RNH201(YNL072W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:SGO1(YOR073W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SLX5(YDL013W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNQ2(YDR011W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUR4(YLR372W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAE1(YBR261C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TMA23(YMR269W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TOK1(YJL093C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TRP2(YER090W)|FD-Score:-5.13|P-value:1.48E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUB3(YML124C)|FD-Score:-3.93|P-value:4.22E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS35(YJL154C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS4(YPR173C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VTC3(YPL019C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:VTS1(YOR359W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YGL176C(YGL176C_p)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR139W(YGR139W_d)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR180W(YHR180W_d)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR146W(YJR146W_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YKR073C(YKR073C_d)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL059C(YLL059C_d)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR097C(YOR097C_p)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR131C(YOR131C_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YSC84(YHR016C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR107C3.334.27E-40.22CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YER009W3.265.57E-40.22NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YGR115C_d3.070.001070.22YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YIR010W2.930.001710.22DSN1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
YHR122W2.920.001770.22CIA2Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
YDR267C2.700.003510.09CIA1Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
YPR165W2.610.004560.02RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YDL008W2.590.004860.00APC11Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
YJL143W2.580.004920.02TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YPL143W2.560.005230.07RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
YPR110C2.490.006420.05RPC40RNA polymerase subunit AC40, common to RNA polymerase I and III
YIL022W2.440.007370.00TIM44Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
YKL089W2.440.007410.06MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YPR142C_d2.380.008630.03YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YOR232W2.360.009240.01MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR011W5.042.35E-7SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YGL219C4.631.82E-6MDM34Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth
YOR374W4.211.30E-5ALD4Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed
YIL012W_d4.072.38E-5YIL012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR038C4.072.39E-5HIR2Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p
YPR173C4.022.89E-5VPS4AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
YOR097C_p3.855.80E-5YOR097C_pPutative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene
YGL136C3.787.94E-5MRM2Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ
YLR047C3.739.75E-5FRE8Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p
YOR321W3.721.01E-4PMT3Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication
YJR146W_p3.671.23E-4YJR146W_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
YNL119W3.641.36E-4NCS2Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
YDR068W3.532.10E-4DOS2Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YBR275C3.532.10E-4RIF1Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YCR017C3.492.43E-4CWH43Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion

GO enrichment analysis for SGTC_1208
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1492.85E-30SGTC_20422-0095 29.0 μMChemDiv (Drug-like library)67908400.140845redox potentiating
0.1095.82E-17SGTC_8590439-0102 54.6 μMChemDiv (Drug-like library)6101430.0972222redox potentiating
0.1016.78E-15SGTC_12111056-0033 32.3 μMChemDiv (Drug-like library)67419220.25
0.1001.67E-14SGTC_12072099-0101 141.0 μMChemDiv (Drug-like library)58373810.197531
0.0831.56E-10SGTC_1615st002656 86.5 μMTimTec (Natural product derivative library)15844580.0886076redox potentiating
0.0741.21E-8SGTC_1097itavastatin 7.2 μMNIH Clinical Collection52824510.121212azole & statin
0.0612.97E-6SGTC_11740739-0097 51.1 μMChemDiv (Drug-like library)7284470.0886076redox potentiating
0.0603.91E-6SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.0909091RNA pol III & RNase P/MRP
0.0561.46E-5SGTC_11383761-0006 1.0 μMChemDiv (Drug-like library)120610870.135135
0.0561.94E-5SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)54979110.0909091calcium & mitochondrial duress
0.0552.15E-5SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.109589superoxide
0.0552.43E-5SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.083333360S ribosome export
0.0552.57E-5SGTC_30349090421 49.5 μMChembridge (Drug-like library)171737610.14634160S ribosome export
0.0542.89E-5SGTC_6704204-0025 119.0 μMChemDiv (Drug-like library)7190390.126582copper-dependent oxidative stress
0.0534.27E-5SGTC_1619st003326 88.7 μMTimTec (Natural product derivative library)28490320.093333360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12092323-234750.3 μM15412760ChemDiv (Drug-like library)346.405664.22724
SGTC_10513253-390378 μM0.4843755509239ChemDiv (Drug-like library)438.105445.44223
SGTC_10523253-3998198 μM0.3888895450317ChemDiv (Drug-like library)370.359322.71127
SGTC_1280906-298113.37 μM0.3787886740226ChemDiv (Drug-like library)296.343682.0124
SGTC_8304076-028657.8 μM0.3580256866659ChemDiv (Drug-like library)411.455844.26825translation
SGTC_13301469-005461.8 μM0.3333337102664ChemDiv (Drug-like library)382.80372.93536iron homeostasis
SGTC_8981000-084928.3 μM0.3283586751807ChemDiv (Drug-like library)356.374042.72334
SGTC_242556286817.85 μM0.3281255714958Miscellaneous259.349964.02715
SGTC_11841269-1718139 μM0.3181825403821ChemDiv (Drug-like library)260.311582.6214
SGTC_11413253-038952.6 μM0.3142865402031ChemDiv (Drug-like library)290.337562.60315