2323-2347

3-naphthalen-2-yl-N-[(Z)-thiophen-2-ylmethylideneamino]-1H-pyrazole-5-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1209
Screen concentration 50.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 5412760
SMILES C1=CC=C2C=C(C=CC2=C1)C3=NNC(=C3)C(=O)NN=CC4=CC=CS4
Standardized SMILES O=C(NN=Cc1cccs1)c2cc([nH]n2)c3ccc4ccccc4c3
Molecular weight 346.4057
ALogP 4.23
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.71
% growth inhibition (Hom. pool) 1.2


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5412760
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.05||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:DBP2(YNL112W)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.02||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DIP2(YLR129W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:EXO84(YBR102C)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.11||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:LSG1(YGL099W)|FD-Score:5.93|P-value:1.49E-9|Clearance:1.81||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM7(YBR202W)|FD-Score:-3.57|P-value:1.76E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NMD3(YHR170W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.07||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RER2(YBR002C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL18A(YOL120C)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN6(YDL097C)|FD-Score:-3.19|P-value:7.12E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.06|P-value:2.47E-5|Clearance:0.21||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:-4.03|P-value:2.74E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:-3.3|P-value:4.92E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-8.35|P-value:3.52E-17|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP46(YGR095C)|FD-Score:-3.46|P-value:2.65E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:-3.3|P-value:4.87E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC9(YML127W)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSM10(YDR041W)|FD-Score:3.85|P-value:6.03E-5|Clearance:0.29||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SEC31(YDL195W)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.19||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC39(YLR440C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.1||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SPB4(YFL002C)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC98(YNL126W)|FD-Score:-3.34|P-value:4.11E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:UTP6(YDR449C)|FD-Score:-5.13|P-value:1.46E-7|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL009W(YJL009W_d)|FD-Score:-4.19|P-value:1.37E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YLR198C(YLR198C_d)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YOR146W(YOR146W_d)|FD-Score:-3.21|P-value:6.74E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ALG7(YBR243C)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.05||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:DBP2(YNL112W)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.02||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DIP2(YLR129W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:EXO84(YBR102C)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.11||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:LSG1(YGL099W)|FD-Score:5.93|P-value:1.49E-9|Clearance:1.81||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM7(YBR202W)|FD-Score:-3.57|P-value:1.76E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NMD3(YHR170W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.07||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RER2(YBR002C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL18A(YOL120C)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN6(YDL097C)|FD-Score:-3.19|P-value:7.12E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.06|P-value:2.47E-5|Clearance:0.21||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:-4.03|P-value:2.74E-5|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:-3.3|P-value:4.92E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-8.35|P-value:3.52E-17|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP46(YGR095C)|FD-Score:-3.46|P-value:2.65E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:-3.3|P-value:4.87E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC9(YML127W)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSM10(YDR041W)|FD-Score:3.85|P-value:6.03E-5|Clearance:0.29||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SEC31(YDL195W)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.19||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC39(YLR440C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.1||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SPB4(YFL002C)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC98(YNL126W)|FD-Score:-3.34|P-value:4.11E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:UTP6(YDR449C)|FD-Score:-5.13|P-value:1.46E-7|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL009W(YJL009W_d)|FD-Score:-4.19|P-value:1.37E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YLR198C(YLR198C_d)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YOR146W(YOR146W_d)|FD-Score:-3.21|P-value:6.74E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5412760
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:5.25|P-value:7.47E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD2(YMR170C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ARG3(YJL088W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL1(YBR164C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG26(YLR189C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BUD32(YGR262C)|FD-Score:-4.99|P-value:3.02E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:COG6(YNL041C)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.47|P-value:2.21E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ELP6(YMR312W)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV14(YGL054C)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ESC1(YMR219W)|FD-Score:-5.5|P-value:1.88E-8||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:EST1(YLR233C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:FCY22(YER060W-A)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FMP27(YLR454W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRM2(YCL026C-A)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:FUB1(YCR076C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:GAT3(YLR013W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GET3(YDL100C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLK1(YCL040W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:IES3(YLR052W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:5.9|P-value:1.84E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JEM1(YJL073W)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LSB1(YGR136W)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress Gene:LTE1(YAL024C)|FD-Score:4.79|P-value:8.51E-7||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MFT1(YML062C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MHR1(YDR296W)|FD-Score:-3.74|P-value:9.36E-5||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:NDJ1(YOL104C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:PHO88(YBR106W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PPA2(YMR267W)|FD-Score:-4.58|P-value:2.36E-6||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:RPN4(YDL020C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS11A(YDR025W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RSM27(YGR215W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SNF6(YHL025W)|FD-Score:-12.8|P-value:1.53E-37||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SUC2(YIL162W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUT1(YGL162W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SYS1(YJL004C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TMA46(YOR091W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP2(YER090W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUB3(YML124C)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VMA7(YGR020C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VTC3(YPL019C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YBL071C(YBL071C_d)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR242W(YBR242W_p)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDR029W(YDR029W_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR094W(YDR094W_d)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YEL008W(YEL008W_d)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YGR228W(YGR228W_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YKR033C(YKR033C_d)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YMR099C(YMR099C)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YOL160W(YOL160W_d)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOP1(YPR028W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR283W(YOR283W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:AFT1(YGL071W)|FD-Score:5.25|P-value:7.47E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD2(YMR170C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ARG3(YJL088W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL1(YBR164C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG26(YLR189C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BUD32(YGR262C)|FD-Score:-4.99|P-value:3.02E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:COG6(YNL041C)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.47|P-value:2.21E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ELP6(YMR312W)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV14(YGL054C)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ESC1(YMR219W)|FD-Score:-5.5|P-value:1.88E-8||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:EST1(YLR233C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:FCY22(YER060W-A)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FMP27(YLR454W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRM2(YCL026C-A)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:FUB1(YCR076C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:GAT3(YLR013W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GET3(YDL100C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLK1(YCL040W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:IES3(YLR052W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:5.9|P-value:1.84E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JEM1(YJL073W)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LSB1(YGR136W)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress Gene:LTE1(YAL024C)|FD-Score:4.79|P-value:8.51E-7||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MFT1(YML062C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MHR1(YDR296W)|FD-Score:-3.74|P-value:9.36E-5||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:NDJ1(YOL104C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:PHO88(YBR106W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PPA2(YMR267W)|FD-Score:-4.58|P-value:2.36E-6||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:RPN4(YDL020C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS11A(YDR025W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RSM27(YGR215W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC6(YPL183W-A)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SNF6(YHL025W)|FD-Score:-12.8|P-value:1.53E-37||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SUC2(YIL162W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUT1(YGL162W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SYS1(YJL004C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TMA46(YOR091W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP2(YER090W)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUB3(YML124C)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBC7(YMR022W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VMA7(YGR020C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VTC3(YPL019C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YBL071C(YBL071C_d)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR242W(YBR242W_p)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDR029W(YDR029W_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR094W(YDR094W_d)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YEL008W(YEL008W_d)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YGR228W(YGR228W_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YKR033C(YKR033C_d)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YMR099C(YMR099C)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YOL160W(YOL160W_d)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOP1(YPR028W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR283W(YOR283W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W5.931.49E-91.81LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W4.131.83E-50.07NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YHR062C4.062.47E-50.21RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR041W3.856.03E-50.29RSM10Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
YDL195W3.551.92E-40.19SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YBR243C3.363.90E-40.05ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YBR102C3.314.60E-40.11EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YNL112W3.206.85E-40.02DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YLR440C3.187.42E-40.10SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YLR029C3.070.001050.07RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YPL151C3.000.001330.08PRP46Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
YER038C2.930.001700.01KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YFL008W2.920.001780.00SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YOR103C2.910.001790.01OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YIL115C2.900.001850.02NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR019C5.901.84E-9IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YMR099C5.813.11E-9YMR099CGlucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
YML071C5.472.21E-8COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL071W5.257.47E-8AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YER090W5.191.04E-7TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YML062C5.191.07E-7MFT1Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
YOL160W_d4.953.69E-7YOL160W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER060W-A4.885.30E-7FCY22Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
YAL024C4.798.51E-7LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YJL004C4.493.58E-6SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YJL073W4.395.63E-6JEM1DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
YNL041C4.385.86E-6COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YCL040W4.112.00E-5GLK1Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
YGL054C4.082.21E-5ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YBR164C4.082.27E-5ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor

GO enrichment analysis for SGTC_1209
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1917.76E-50SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.064516160S ribosome export
0.1822.93E-45SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.11538560S ribosome export
0.1701.29E-39SGTC_11861319-0216 26.0 μMChemDiv (Drug-like library)45651360.0957447
0.1696.46E-39SGTC_14043909-7820 48.3 μMChemDiv (Drug-like library)237413730.12195160S ribosome export
0.1598.90E-35SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.109589Golgi
0.1591.41E-34SGTC_21005377183 190.0 μMChembridge (Fragment library)28442020.076923160S ribosome export
0.1584.23E-34SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.147059
0.1574.88E-34SGTC_1090132-0036 33.7 μMChemDiv (Drug-like library)672740.197183
0.1521.05E-31SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.144928Golgi
0.1491.04E-30SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0886076PDR1
0.1446.11E-29SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.10256460S ribosome export
0.1399.50E-27SGTC_13631636-0063 10.4 μMChemDiv (Drug-like library)60721850.246377Golgi
0.1381.94E-26SGTC_2645abamectin 100.0 μMMicrosource (Natural product library)57022080.0206897heme requiring
0.1373.09E-26SGTC_31019120531 49.5 μMChembridge (Drug-like library)248249040.0941176
0.1373.87E-26SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.106667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12082323-234750.3 μM15412760ChemDiv (Drug-like library)346.405664.22724
SGTC_10513253-390378 μM0.4843755509239ChemDiv (Drug-like library)438.105445.44223
SGTC_10523253-3998198 μM0.3888895450317ChemDiv (Drug-like library)370.359322.71127
SGTC_1280906-298113.37 μM0.3787886740226ChemDiv (Drug-like library)296.343682.0124
SGTC_8304076-028657.8 μM0.3580256866659ChemDiv (Drug-like library)411.455844.26825translation
SGTC_13301469-005461.8 μM0.3333337102664ChemDiv (Drug-like library)382.80372.93536iron homeostasis
SGTC_8981000-084928.3 μM0.3283586751807ChemDiv (Drug-like library)356.374042.72334
SGTC_242556286817.85 μM0.3281255714958Miscellaneous259.349964.02715
SGTC_11841269-1718139 μM0.3181825403821ChemDiv (Drug-like library)260.311582.6214
SGTC_11413253-038952.6 μM0.3142865402031ChemDiv (Drug-like library)290.337562.60315