3013-0140

1,6-dimethyl-3-thiophen-2-ylpyrimido[5,4-e][1,2,4]triazine-5,7-dione

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1210
Screen concentration 750.0 nM
Source ChemDiv (Drug-like library)
PubChem CID 905973
SMILES CN1C2=NC(=O)N(C(=O)C2=NC(=N1)C3=CC=CS3)C
Standardized SMILES CN1N=C(N=C2C(=O)N(C)C(=O)N=C12)c3cccs3
Molecular weight 275.2865
ALogP 1.18
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.72
% growth inhibition (Hom. pool) 1.2


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 905973
Download HIP data (tab-delimited text)  (excel)
Gene:CDC33(YOL139C)|FD-Score:3.1|P-value:9.79E-4|Clearance:0.08||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:DPB2(YPR175W)|FD-Score:3.26|P-value:5.59E-4|Clearance:0.16||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ERG7(YHR072W)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.7||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:ERG8(YMR220W)|FD-Score:-4.33|P-value:7.31E-6|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LCP5(YER127W)|FD-Score:6.41|P-value:7.26E-11|Clearance:2.25||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MCM3(YEL032W)|FD-Score:-3.4|P-value:3.37E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MTR10(YOR160W)|FD-Score:4.16|P-value:1.59E-5|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NSA1(YGL111W)|FD-Score:-3.45|P-value:2.79E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:RKI1(YOR095C)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.12||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:SPC98(YNL126W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.08||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:YDR396W(YDR396W_d)|FD-Score:-3.82|P-value:6.79E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:CDC33(YOL139C)|FD-Score:3.1|P-value:9.79E-4|Clearance:0.08||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:DPB2(YPR175W)|FD-Score:3.26|P-value:5.59E-4|Clearance:0.16||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ERG7(YHR072W)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.7||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:ERG8(YMR220W)|FD-Score:-4.33|P-value:7.31E-6|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LCP5(YER127W)|FD-Score:6.41|P-value:7.26E-11|Clearance:2.25||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MCM3(YEL032W)|FD-Score:-3.4|P-value:3.37E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MTR10(YOR160W)|FD-Score:4.16|P-value:1.59E-5|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NSA1(YGL111W)|FD-Score:-3.45|P-value:2.79E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:RKI1(YOR095C)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.12||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:SPC98(YNL126W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.08||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:YDR396W(YDR396W_d)|FD-Score:-3.82|P-value:6.79E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 905973
Download HOP data (tab-delimited text)  (excel)
Gene:DIT2(YDR402C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EFT2(YDR385W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:EHT1(YBR177C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FRE8(YLR047C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FUR4(YBR021W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HSP42(YDR171W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HXT10(YFL011W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IRC21(YMR073C)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KTR2(YKR061W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LCB4(YOR171C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LTE1(YAL024C)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MET18(YIL128W)|FD-Score:-3.89|P-value:4.97E-5||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MRPL51(YPR100W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN1(YOL116W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MUS81(YDR386W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PET100(YDR079W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PIG2(YIL045W)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:RAD61(YDR014W)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RGS2(YOR107W)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RPS17B(YDR447C)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPR6(YER115C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SSD1(YDR293C)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP22(YCL008C)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TEX1(YNL253W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:VAN1(YML115C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VTC2(YFL004W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YAL066W(YAL066W_d)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL065W(YBL065W_d)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YDL218W(YDL218W_p)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR102C(YDR102C_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR269C(YDR269C_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR431W(YDR431W_d)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR137W(YGR137W_d)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR281C(YLR281C_p)|FD-Score:4.45|P-value:4.24E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YML122C(YML122C_d)|FD-Score:-5.21|P-value:9.39E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR187C(YMR187C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YOP1(YPR028W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR316C-A(YOR316C-A_p)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL257W(YPL257W_p)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:DIT2(YDR402C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EFT2(YDR385W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:EHT1(YBR177C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FRE8(YLR047C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FUR4(YBR021W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HSP42(YDR171W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HXT10(YFL011W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IRC21(YMR073C)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KTR2(YKR061W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LCB4(YOR171C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LTE1(YAL024C)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MET18(YIL128W)|FD-Score:-3.89|P-value:4.97E-5||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MRPL51(YPR100W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN1(YOL116W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MUS81(YDR386W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PET100(YDR079W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PIG2(YIL045W)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:RAD61(YDR014W)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RGS2(YOR107W)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RPS17B(YDR447C)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPR6(YER115C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SSD1(YDR293C)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP22(YCL008C)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TEX1(YNL253W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:VAN1(YML115C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VTC2(YFL004W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YAL066W(YAL066W_d)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL065W(YBL065W_d)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YDL218W(YDL218W_p)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR102C(YDR102C_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR269C(YDR269C_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR431W(YDR431W_d)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR137W(YGR137W_d)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR281C(YLR281C_p)|FD-Score:4.45|P-value:4.24E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YML122C(YML122C_d)|FD-Score:-5.21|P-value:9.39E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR187C(YMR187C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YOP1(YPR028W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR316C-A(YOR316C-A_p)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL257W(YPL257W_p)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER127W6.417.26E-112.25LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YOR160W4.161.59E-50.00MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YHR072W4.161.60E-50.69ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YNL126W3.462.67E-40.08SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YOR095C3.383.64E-40.12RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YPR175W3.265.59E-40.16DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YOL139C3.109.79E-40.08CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YNL310C3.020.001260.04ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YFL024C2.990.001420.08EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YPR107C2.900.001840.01YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YFR003C2.890.001940.03YPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
YER012W2.860.002150.08PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YOL066C2.780.002730.10RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YDR328C2.680.003680.05SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YKL060C2.630.004250.01FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR281C_p4.454.24E-6YLR281C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene
YDR431W_d4.211.27E-5YDR431W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR402C4.121.93E-5DIT2N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s
YBR021W4.022.94E-5FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YOL116W3.973.62E-5MSN1Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YDR014W3.768.34E-5RAD61Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin
YOR008C-A_p3.701.06E-4YOR008C-A_pPutative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
YPR100W3.701.09E-4MRPL51Mitochondrial ribosomal protein of the large subunit
YBR177C3.671.21E-4EHT1Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication
YDR102C_d3.601.59E-4YDR102C_dDubious open reading frame; homozygous diploid deletion strain exhibits high budding index
YLR047C3.591.67E-4FRE8Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p
YOR171C3.591.67E-4LCB4Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
YFL011W3.571.79E-4HXT10Putative hexose transporter, expressed at low levels and expression is repressed by glucose
YML115C3.551.96E-4VAN1Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
YDR433W_d3.472.58E-4YDR433W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1210
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0952.15E-13SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0724638ergosterol biosynthesis
0.0912.89E-12SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.111111RNA processing & uracil transport
0.0758.40E-9SGTC_5554296-0685 37.0 μMChemDiv (Drug-like library)29085400.164384ergosterol biosynthesis
0.0714.45E-8SGTC_12072099-0101 141.0 μMChemDiv (Drug-like library)58373810.0617284
0.0706.77E-8SGTC_12111056-0033 32.3 μMChemDiv (Drug-like library)67419220.0595238
0.0699.59E-8SGTC_12370330-0133 46.1 μMChemDiv (Drug-like library)X12370.0597015ergosterol biosynthesis
0.0681.38E-7SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.0769231ergosterol biosynthesis
0.0672.36E-7SGTC_1033k072-0102 21.3 μMChemDiv (Drug-like library)59659310.13043560S ribosome export
0.0672.67E-7SGTC_1167k781-0610 148.0 μMChemDiv (Drug-like library)45251690.1RNA processing & uracil transport
0.0641.03E-6SGTC_14984478-3555 97.6 μMChemDiv (Drug-like library)34379190.0972222
0.0631.43E-6SGTC_21956536160 144.2 μMChembridge (Fragment library)38343860.086206960S ribosome export
0.0631.46E-6SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.0869565RNA processing & uracil transport
0.0612.38E-6SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.111111RNA processing & uracil transport
0.0604.09E-6SGTC_23459052315 157.4 μMChembridge (Fragment library)64692650.111111
0.0561.89E-5SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.0923077RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1513013-01442.29 μM0.403509906558ChemDiv (Drug-like library)313.31131.56304iron homeostasis
SGTC_6721441-001853.7 μM0.25251141ChemDiv (Drug-like library)212.270323.19403
SGTC_21465545642200 μM0.25825326Chembridge (Fragment library)202.232441.21713
SGTC_21685727979200 μM0.240741695210Chembridge (Fragment library)206.26422.23603
SGTC_1799st04582246.14 μM0.2368423674992TimTec (Natural product derivative library)349.42792.42606
SGTC_22087283366200 μM0.236364898663Chembridge (Fragment library)224.302621.34324
SGTC_3354077-025627.6 μM0.2318843121989ChemDiv (Drug-like library)363.27213.69404
SGTC_1982st07246771.9 μM0.2295083163533TimTec (Natural product derivative library)278.32513.99803
SGTC_1780st03844824.54 μM0.225806728868TimTec (Natural product derivative library)278.710883.21614iron homeostasis
SGTC_3306912767659.79 μM0.22580641905287Chembridge (Drug-like library)264.369783.04205