0358-0031

4-amino-3-methyl-1-phenylthieno[2,3-c]pyrazole-5-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1214
Screen concentration 63.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 4301366
SMILES CC1=NN(C2=C1C(=C(S2)C#N)N)C3=CC=CC=C3
Standardized SMILES Cc1nn(c2ccccc2)c3sc(C#N)c(N)c13
Molecular weight 254.3103
ALogP 2.5
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 30.31
% growth inhibition (Hom. pool) 16.8


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4301366
Download HIP data (tab-delimited text)  (excel)
Gene:AOS1(YPR180W)|FD-Score:-11.9|P-value:3.56E-33|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:BMS1(YPL217C)|FD-Score:3.74|P-value:9.22E-5|Clearance:0.28||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC39(YCR093W)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.06||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:COF1(YLL050C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.01||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:DYS1(YHR068W)|FD-Score:-3.14|P-value:8.51E-4|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:KOG1(YHR186C)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.09||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:PCF11(YDR228C)|FD-Score:4.01|P-value:2.98E-5|Clearance:0.03||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PGI1(YBR196C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.18||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PSF2(YJL072C)|FD-Score:-3.33|P-value:4.41E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PTI1(YGR156W)|FD-Score:4.54|P-value:2.86E-6|Clearance:0.35||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RAD53(YPL153C)|FD-Score:-3.17|P-value:7.53E-4|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RPC11(YDR045C)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL15A(YLR029C)|FD-Score:5.68|P-value:6.69E-9|Clearance:1.14||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:TAF1(YGR274C)|FD-Score:4.14|P-value:1.71E-5|Clearance:0.1||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TIM23(YNR017W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.19||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:YDL163W(YDL163W_d)|FD-Score:4.04|P-value:2.64E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGL069C(YGL069C_d)|FD-Score:-4.44|P-value:4.45E-6|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR115C(YGR115C_d)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YKL036C(YKL036C_d)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YNL181W(YNL181W_p)|FD-Score:-3.76|P-value:8.50E-5|Clearance:0||SGD DESC:Putative oxidoreductase; required for cell viability Gene:YNL247W(YNL247W)|FD-Score:3.93|P-value:4.24E-5|Clearance:0.1||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:AOS1(YPR180W)|FD-Score:-11.9|P-value:3.56E-33|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:BMS1(YPL217C)|FD-Score:3.74|P-value:9.22E-5|Clearance:0.28||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC39(YCR093W)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.06||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:COF1(YLL050C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.01||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:DYS1(YHR068W)|FD-Score:-3.14|P-value:8.51E-4|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:KOG1(YHR186C)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.09||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:PCF11(YDR228C)|FD-Score:4.01|P-value:2.98E-5|Clearance:0.03||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PGI1(YBR196C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.18||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PSF2(YJL072C)|FD-Score:-3.33|P-value:4.41E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PTI1(YGR156W)|FD-Score:4.54|P-value:2.86E-6|Clearance:0.35||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RAD53(YPL153C)|FD-Score:-3.17|P-value:7.53E-4|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RPC11(YDR045C)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL15A(YLR029C)|FD-Score:5.68|P-value:6.69E-9|Clearance:1.14||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:TAF1(YGR274C)|FD-Score:4.14|P-value:1.71E-5|Clearance:0.1||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TIM23(YNR017W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.19||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:YDL163W(YDL163W_d)|FD-Score:4.04|P-value:2.64E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGL069C(YGL069C_d)|FD-Score:-4.44|P-value:4.45E-6|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR115C(YGR115C_d)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YKL036C(YKL036C_d)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YNL181W(YNL181W_p)|FD-Score:-3.76|P-value:8.50E-5|Clearance:0||SGD DESC:Putative oxidoreductase; required for cell viability Gene:YNL247W(YNL247W)|FD-Score:3.93|P-value:4.24E-5|Clearance:0.1||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4301366
Download HOP data (tab-delimited text)  (excel)
Gene:ACK1(YDL203C)|FD-Score:7.16|P-value:4.17E-13||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ADE1(YAR015W)|FD-Score:4.76|P-value:9.46E-7||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AKR1(YDR264C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:APE3(YBR286W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARP8(YOR141C)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:AVO2(YMR068W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BAS1(YKR099W)|FD-Score:-12.8|P-value:8.80E-38||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:CHA4(YLR098C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:COA2(YPL189C-A)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CTA1(YDR256C)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CTR1(YPR124W)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CWH41(YGL027C)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DIA3(YDL024C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DOA1(YKL213C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DSD1(YGL196W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:DXO1(YDR370C_p)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:EAF6(YJR082C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM7(YLR443W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:FMP46(YKR049C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAL7(YBR018C)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GLY1(YEL046C)|FD-Score:-3.59|P-value:1.69E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GYP1(YOR070C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HIS7(YBR248C)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HOS1(YPR068C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:ICY2(YPL250C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:LSC1(YOR142W)|FD-Score:-6.87|P-value:3.15E-12||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MKT1(YNL085W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:NBP2(YDR162C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NIT3(YLR351C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:PDR11(YIL013C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PKH1(YDR490C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PMT7(YDR307W_p)|FD-Score:-5.38|P-value:3.73E-8||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PRB1(YEL060C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRS2(YER099C)|FD-Score:5.15|P-value:1.29E-7||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTP3(YER075C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:RAD57(YDR004W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAV1(YJR033C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCN1(YKL159C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REF2(YDR195W)|FD-Score:-4.03|P-value:2.75E-5||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RPL22B(YFL034C-A)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAS5(YOR213C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SEC22(YLR268W)|FD-Score:-4.33|P-value:7.51E-6||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHS1(YDL225W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SPO21(YOL091W)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SSA2(YLL024C)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STE13(YOR219C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SYS1(YJL004C)|FD-Score:8.83|P-value:5.43E-19||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TES1(YJR019C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TFB6(YOR352W)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP2(YER090W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBC5(YDR059C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VBA1(YMR088C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS1(YKR001C)|FD-Score:-5.63|P-value:9.25E-9||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YAR028W(YAR028W_p)|FD-Score:-4.92|P-value:4.26E-7||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YCK3(YER123W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR023C(YCR023C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR029W(YDR029W_d)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL262W(YGL262W_p)|FD-Score:4.43|P-value:4.77E-6||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YGR210C(YGR210C_p)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR130C(YHR130C_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM2(YMR151W_d)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YJR003C(YJR003C_p)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YLR040C(YLR040C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR202C(YLR202C_d)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR181C(YMR181C_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YMR320W(YMR320W_d)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL170W(YNL170W_d)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL198C(YNL198C_d)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Putative protein of unknown function Gene:YNL295W(YNL295W_p)|FD-Score:-5.57|P-value:1.24E-8||SGD DESC:Putative protein of unknown function Gene:YOL159C-A(YOL159C-A)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YPL168W(YPL168W_p)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPL185W(YPL185W_d)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR064W(YPR064W_d)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACK1(YDL203C)|FD-Score:7.16|P-value:4.17E-13||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ADE1(YAR015W)|FD-Score:4.76|P-value:9.46E-7||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AKR1(YDR264C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:APE3(YBR286W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARP8(YOR141C)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:AVO2(YMR068W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BAS1(YKR099W)|FD-Score:-12.8|P-value:8.80E-38||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:CHA4(YLR098C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:COA2(YPL189C-A)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CTA1(YDR256C)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CTR1(YPR124W)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CWH41(YGL027C)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DIA3(YDL024C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DOA1(YKL213C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DSD1(YGL196W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:DXO1(YDR370C_p)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:EAF6(YJR082C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM7(YLR443W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:FMP46(YKR049C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAL7(YBR018C)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GLY1(YEL046C)|FD-Score:-3.59|P-value:1.69E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GYP1(YOR070C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HIS7(YBR248C)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HOS1(YPR068C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:ICY2(YPL250C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:LSC1(YOR142W)|FD-Score:-6.87|P-value:3.15E-12||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MKT1(YNL085W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:NBP2(YDR162C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NIT3(YLR351C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:PDR11(YIL013C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PKH1(YDR490C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PMT7(YDR307W_p)|FD-Score:-5.38|P-value:3.73E-8||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PRB1(YEL060C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRS2(YER099C)|FD-Score:5.15|P-value:1.29E-7||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTP3(YER075C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:RAD57(YDR004W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAV1(YJR033C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCN1(YKL159C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REF2(YDR195W)|FD-Score:-4.03|P-value:2.75E-5||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RPL22B(YFL034C-A)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAS5(YOR213C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SEC22(YLR268W)|FD-Score:-4.33|P-value:7.51E-6||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHS1(YDL225W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SPO21(YOL091W)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SSA2(YLL024C)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STE13(YOR219C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SYS1(YJL004C)|FD-Score:8.83|P-value:5.43E-19||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TES1(YJR019C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TFB6(YOR352W)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP2(YER090W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBC5(YDR059C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VBA1(YMR088C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS1(YKR001C)|FD-Score:-5.63|P-value:9.25E-9||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YAR028W(YAR028W_p)|FD-Score:-4.92|P-value:4.26E-7||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YCK3(YER123W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR023C(YCR023C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR029W(YDR029W_d)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL262W(YGL262W_p)|FD-Score:4.43|P-value:4.77E-6||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YGR210C(YGR210C_p)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR130C(YHR130C_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM2(YMR151W_d)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YJR003C(YJR003C_p)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YLR040C(YLR040C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR202C(YLR202C_d)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR181C(YMR181C_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YMR320W(YMR320W_d)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL170W(YNL170W_d)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL198C(YNL198C_d)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Putative protein of unknown function Gene:YNL295W(YNL295W_p)|FD-Score:-5.57|P-value:1.24E-8||SGD DESC:Putative protein of unknown function Gene:YOL159C-A(YOL159C-A)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YPL168W(YPL168W_p)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPL185W(YPL185W_d)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR064W(YPR064W_d)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR029C5.686.69E-91.14RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YGR156W4.542.86E-60.35PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YGR115C_d4.191.39E-50.04YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YLL050C4.151.66E-50.01COF1Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
YGR274C4.141.71E-50.10TAF1TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
YDL163W_d4.042.64E-50.03YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YDR228C4.012.98E-50.03PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YCR093W3.993.34E-50.06CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YNL247W3.934.24E-50.10YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YHR186C3.836.46E-50.09KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YPL217C3.749.22E-50.28BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YNR017W3.462.66E-40.19TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
YBR196C3.285.26E-40.18PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YKL036C_d3.109.71E-40.12YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YJR112W2.980.001440.01NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL004C8.835.43E-19SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YDL203C7.164.17E-13ACK1Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
YOL091W5.601.10E-8SPO21Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
YER099C5.151.29E-7PRS25-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
YAR015W4.769.46E-7ADE1N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
YFL034C-A4.542.79E-6RPL22BRibosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
YLL024C4.464.09E-6SSA2ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes
YGL262W_p4.434.77E-6YGL262W_pPutative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene
YJR082C4.191.37E-5EAF6Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
YMR181C_p4.151.66E-5YMR181C_pProtein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication
YNL085W4.141.77E-5MKT1Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress
YGR210C_p4.092.11E-5YGR210C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YLR351C4.072.34E-5NIT3Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
YNL170W_d4.072.36E-5YNL170W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL041C-B_p4.062.47E-5YGL041C-B_pPutative protein of unknown function; identified by fungal homology and RT-PCR

GO enrichment analysis for SGTC_1214
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1323.16E-24SGTC_12131315-0166 115.0 μMChemDiv (Drug-like library)7684010.0985916
0.1048.89E-16SGTC_12120520-0165 585.0 μMChemDiv (Drug-like library)54032580.0606061
0.0822.64E-10SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.0923077
0.0723.85E-8SGTC_1461k072-0203 53.8 μMChemDiv (Drug-like library)54187140.129032
0.0713.81E-8SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.0322581
0.0657.70E-7SGTC_1463k081-0032 34.1 μMChemDiv (Drug-like library)9777550.0704225
0.0631.38E-6SGTC_7273032-0951 202.0 μMChemDiv (Drug-like library)36528360.115942
0.0603.85E-6SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0705882cell wall
0.0595.34E-6SGTC_30679117431 49.5 μMChembridge (Drug-like library)171508850.0895522
0.0596.39E-6SGTC_1905st060207 84.5 μMTimTec (Natural product derivative library)19485470.0724638
0.0571.01E-5SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.125cell wall
0.0571.05E-5SGTC_30039075612 71.4 μMChembridge (Drug-like library)65019930.0933333
0.0571.29E-5SGTC_23739071805 200.0 μMChembridge (Fragment library)101490090.16
0.0552.09E-5SGTC_31019120531 49.5 μMChembridge (Drug-like library)248249040.140845
0.0552.16E-5SGTC_8780828-0289 13.7 μMChemDiv (Drug-like library)7453500.1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21635671233125 μM0.339286701023Chembridge (Fragment library)233.289541.2515
SGTC_250118-022335.66 μM0.3090915333628ChemDiv (Drug-like library)294.307960.9225
SGTC_13802291-0730164 μM0.2698412999191ChemDiv (Drug-like library)334.394964.25814
SGTC_3274913829849.47 μM0.26666717015756Chembridge (Drug-like library)293.32322.66624
SGTC_1923455-060960.9 μM0.2653063769639ChemDiv (Drug-like library)193.652763.52602
SGTC_270485-000132.62 μM0.2407412054479ChemDiv (Drug-like library)266.360764.32313RPP1 & pyrimidine depletion
SGTC_13521496-099199 μM0.2407413000039ChemDiv (Drug-like library)215.274261.98603tubulin folding & SWR complex
SGTC_2092530369480.67 μM0.2363641963699Chembridge (Fragment library)274.36461.35126TSC3-RPN4
SGTC_20865274770176.62 μM0.235294689278Chembridge (Fragment library)254.31031.46325
SGTC_3225913081949.47 μM0.2343754547121Chembridge (Drug-like library)293.32322.66624amide catabolism