1348-1416

N-[(2-oxoindol-3-yl)amino]-N'-pyridin-2-ylpyridine-2-carboximidamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1218
Screen concentration 30.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 6787668
SMILES C1=CC=NC(=C1)C(=NC2=CC=CC=N2)NNC3=C4C=CC=CC4=NC3=O
Standardized SMILES Oc1[nH]c2ccccc2c1N=NC(=Nc3ccccn3)c4ccccn4
Molecular weight 342.3541
ALogP 1.62
H-bond donor count 2
H-bond acceptor count 5
Response signature SWF1 & branched chain AA biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.53
% growth inhibition (Hom. pool) 1.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6787668
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:CDC12(YHR107C)|FD-Score:3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CLP1(YOR250C)|FD-Score:4.27|P-value:9.59E-6|Clearance:0.36||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DBP10(YDL031W)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.13||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DPB11(YJL090C)|FD-Score:4.95|P-value:3.79E-7|Clearance:0.67||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ENP1(YBR247C)|FD-Score:-3.18|P-value:7.33E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:EPL1(YFL024C)|FD-Score:-3.8|P-value:7.23E-5|Clearance:0||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GLC7(YER133W)|FD-Score:3.28|P-value:5.11E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI16(YHR188C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:HEM4(YOR278W)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.02||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:LCB1(YMR296C)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.04||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MED4(YOR174W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MGE1(YOR232W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.01||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:REB1(YBR049C)|FD-Score:3.09|P-value:9.97E-4|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPS15(YOL040C)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC6(YCR052W)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC18(YBR080C)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Gene:SEC27(YGL137W)|FD-Score:3.79|P-value:7.62E-5|Clearance:0.48||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SNU114(YKL173W)|FD-Score:-3.11|P-value:9.33E-4|Clearance:0||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SPT15(YER148W)|FD-Score:-4.3|P-value:8.38E-6|Clearance:0||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SPT16(YGL207W)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.14||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TIM44(YIL022W)|FD-Score:-3.59|P-value:1.66E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:UTP10(YJL109C)|FD-Score:-7.21|P-value:2.79E-13|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:UTP9(YHR196W)|FD-Score:-3.45|P-value:2.77E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ACP1(YKL192C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:CDC12(YHR107C)|FD-Score:3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CLP1(YOR250C)|FD-Score:4.27|P-value:9.59E-6|Clearance:0.36||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DBP10(YDL031W)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.13||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DPB11(YJL090C)|FD-Score:4.95|P-value:3.79E-7|Clearance:0.67||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ENP1(YBR247C)|FD-Score:-3.18|P-value:7.33E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:EPL1(YFL024C)|FD-Score:-3.8|P-value:7.23E-5|Clearance:0||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GLC7(YER133W)|FD-Score:3.28|P-value:5.11E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI16(YHR188C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:HEM4(YOR278W)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.02||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:LCB1(YMR296C)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.04||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MED4(YOR174W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MGE1(YOR232W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.01||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:REB1(YBR049C)|FD-Score:3.09|P-value:9.97E-4|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPS15(YOL040C)|FD-Score:-3.17|P-value:7.58E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC6(YCR052W)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC18(YBR080C)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Gene:SEC27(YGL137W)|FD-Score:3.79|P-value:7.62E-5|Clearance:0.48||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SNU114(YKL173W)|FD-Score:-3.11|P-value:9.33E-4|Clearance:0||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SPT15(YER148W)|FD-Score:-4.3|P-value:8.38E-6|Clearance:0||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SPT16(YGL207W)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.14||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TIM44(YIL022W)|FD-Score:-3.59|P-value:1.66E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:UTP10(YJL109C)|FD-Score:-7.21|P-value:2.79E-13|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:UTP9(YHR196W)|FD-Score:-3.45|P-value:2.77E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6787668
Download HOP data (tab-delimited text)  (excel)
Gene:AIM44(YPL158C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:APE4(YHR113W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ARF3(YOR094W)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ARO1(YDR127W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP8(YOR141C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG18(YFR021W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP25(YMR098C)|FD-Score:4.33|P-value:7.41E-6||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BLM10(YFL007W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BTS1(YPL069C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD9(YGR041W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole Gene:CHS5(YLR330W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:DUG2(YBR281C)|FD-Score:3.81|P-value:7.09E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF6(YJR082C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM14(YHR132C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:FMP27(YLR454W_p)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FOB1(YDR110W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:GEP4(YHR100C)|FD-Score:4.49|P-value:3.51E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GYP1(YOR070C)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HUT1(YPL244C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:IRC6(YFR043C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LSC2(YGR244C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MDH3(YDL078C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MDS3(YGL197W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIP1(YOR330C)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MNN5(YJL186W)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:OSH6(YKR003W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PEP4(YPL154C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PHB1(YGR132C)|FD-Score:-3.85|P-value:5.85E-5||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PIG1(YLR273C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PSY2(YNL201C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RPL12A(YEL054C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL16B(YNL069C)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.27|P-value:1.76E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRF1(YHR038W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RTA1(YGR213C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SLM4(YBR077C)|FD-Score:4.85|P-value:6.23E-7||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SPF1(YEL031W)|FD-Score:7.81|P-value:2.95E-15||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPS100(YHR139C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSD1(YDR293C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB5(YHR178W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWF1(YDR126W)|FD-Score:9.24|P-value:1.20E-20||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRM44(YPL030W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VMA13(YPR036W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR219C(YBR219C_p)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDR015C(YDR015C_d)|FD-Score:-5.74|P-value:4.83E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YGL015C(YGL015C_p)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGP1(YNL160W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YIL025C(YIL025C_d)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL067C(YIL067C_p)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Uncharacterized protein of unknown function Gene:YJL027C(YJL027C_p)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function Gene:YJR011C(YJR011C_p)|FD-Score:-3.74|P-value:9.37E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YKL169C(YKL169C_d)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR232W(YLR232W_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YLR252W(YLR252W_d)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR446W(YLR446W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML037C(YML037C_p)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YMR114C(YMR114C)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL195C(YNL195C_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL131W(YOL131W_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YPL257W(YPL257W_p)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:AIM44(YPL158C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:APE4(YHR113W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ARF3(YOR094W)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ARO1(YDR127W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP8(YOR141C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG18(YFR021W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP25(YMR098C)|FD-Score:4.33|P-value:7.41E-6||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BLM10(YFL007W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BTS1(YPL069C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD9(YGR041W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole Gene:CHS5(YLR330W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:DUG2(YBR281C)|FD-Score:3.81|P-value:7.09E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF6(YJR082C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM14(YHR132C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:FMP27(YLR454W_p)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FOB1(YDR110W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:GEP4(YHR100C)|FD-Score:4.49|P-value:3.51E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GYP1(YOR070C)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HUT1(YPL244C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:IRC6(YFR043C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LSC2(YGR244C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MDH3(YDL078C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MDS3(YGL197W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIP1(YOR330C)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MNN5(YJL186W)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:OSH6(YKR003W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PEP4(YPL154C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PHB1(YGR132C)|FD-Score:-3.85|P-value:5.85E-5||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PIG1(YLR273C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PSY2(YNL201C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RPL12A(YEL054C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL16B(YNL069C)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.27|P-value:1.76E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRF1(YHR038W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RTA1(YGR213C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SLM4(YBR077C)|FD-Score:4.85|P-value:6.23E-7||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SPF1(YEL031W)|FD-Score:7.81|P-value:2.95E-15||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPS100(YHR139C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSD1(YDR293C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB5(YHR178W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWF1(YDR126W)|FD-Score:9.24|P-value:1.20E-20||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRM44(YPL030W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VMA13(YPR036W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR219C(YBR219C_p)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDR015C(YDR015C_d)|FD-Score:-5.74|P-value:4.83E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YGL015C(YGL015C_p)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGP1(YNL160W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YIL025C(YIL025C_d)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL067C(YIL067C_p)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Uncharacterized protein of unknown function Gene:YJL027C(YJL027C_p)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function Gene:YJR011C(YJR011C_p)|FD-Score:-3.74|P-value:9.37E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YKL169C(YKL169C_d)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR232W(YLR232W_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YLR252W(YLR252W_d)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR446W(YLR446W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML037C(YML037C_p)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YMR114C(YMR114C)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL195C(YNL195C_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL131W(YOL131W_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YPL257W(YPL257W_p)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YJL090C4.953.79E-70.67DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YOR250C4.279.59E-60.36CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YDL031W3.924.51E-50.13DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YGL137W3.797.62E-50.48SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YOR278W3.314.68E-40.02HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YOR232W3.294.98E-40.01MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YER133W3.285.11E-40.00GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YOR174W3.285.13E-40.01MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YGL207W3.275.32E-40.14SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YHR107C3.148.54E-40.00CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YMR296C3.138.62E-40.04LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YBR049C3.099.97E-40.02REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YGR186W3.070.001070.02TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YLR457C3.050.001130.13NBP1Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
YLR440C2.920.001750.09SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR126W9.241.20E-20SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YEL031W7.812.95E-15SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YDL020C6.271.76E-10RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR454W_p5.383.68E-8FMP27_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBR077C4.856.23E-7SLM4Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
YFR043C4.691.34E-6IRC6Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
YNL069C4.542.82E-6RPL16BRibosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication
YHR100C4.493.51E-6GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YBR174C_d4.464.18E-6YBR174C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective
YMR098C4.337.41E-6ATP25Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
YMR114C4.337.48E-6YMR114CProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
YDR293C4.032.76E-5SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YPR036W4.022.93E-5VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YGL015C_p3.944.14E-5YGL015C_pPutative protein of unknown function; null mutants accumulate cargo in the Golgi
YJL186W3.894.95E-5MNN5Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment

GO enrichment analysis for SGTC_1218
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1776.25E-43SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.105263SWF1 & branched chain AA biosynthesis
0.1561.93E-33SGTC_340469-0683 81.8 μMChemDiv (Drug-like library)36909230.0666667SWF1 & branched chain AA biosynthesis
0.1474.87E-30SGTC_20435101342 149.0 μMChembridge (Fragment library)6784510.0410959SWF1 & branched chain AA biosynthesis
0.1361.14E-25SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.109756
0.1345.60E-25SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.0810811SWF1 & branched chain AA biosynthesis
0.1313.97E-24SGTC_20345197015 53.1 μMChembridge (Fragment library)7806760.126761SWF1 & branched chain AA biosynthesis
0.1316.29E-24SGTC_9841492-0255 2.9 μMChemDiv (Drug-like library)15851980.0985916SWF1 & branched chain AA biosynthesis
0.1284.72E-23SGTC_7961234-0020 70.7 μMChemDiv (Drug-like library)464950340.0909091SWF1 & branched chain AA biosynthesis
0.1224.77E-21SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.0819672SWF1 & branched chain AA biosynthesis
0.1181.03E-19SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.163934heme biosynthesis & mitochondrial translocase
0.1095.86E-17SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.0886076
0.0984.81E-14SGTC_2659sanguinarine 24.2 μMMicrosource (Natural product library)133941490.0384615
0.0961.11E-13SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0972222
0.0897.16E-12SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.106061
0.0899.16E-12SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.106061

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6221348-156626.3 μM0.4482767340407ChemDiv (Drug-like library)370.235324.51514
SGTC_13231348-135111.2 μM0.4153856989654ChemDiv (Drug-like library)336.304882.617
SGTC_9771348-1378135 μM0.402985X977ChemDiv (Drug-like library)362.342162.83982
SGTC_10451348-1485165 μM0.3802826810614ChemDiv (Drug-like library)396.787221.62127
SGTC_289k017-0060526 μM0.3442625512909ChemDiv (Drug-like library)380.21651.56725
SGTC_9031134-0042114 μM0.314815701009ChemDiv (Drug-like library)229.300843.75522
SGTC_20565235370200 μM0.3114755511916Chembridge (Fragment library)237.301381.57125
SGTC_7491000-0129128 μM0.310345708470ChemDiv (Drug-like library)257.310943.15423amide catabolism
SGTC_9473447-0010211 μM0.295082748350ChemDiv (Drug-like library)238.24142.59813
SGTC_22717849410118.62 μM0.269841972430Chembridge (Fragment library)233.289542.66623