0823-0416

[4-(5-methylsulfanyl-1,3,4-oxadiazol-2-yl)phenyl] thiocyanate

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1224
Screen concentration 294.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3598164
SMILES CSC1=NN=C(O1)C2=CC=C(C=C2)SC#N
Standardized SMILES CSc1oc(nn1)c2ccc(SC#N)cc2
Molecular weight 249.3121
ALogP 2.69
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -2.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3598164
Download HIP data (tab-delimited text)  (excel)
Gene:CDC5(YMR001C)|FD-Score:-3.93|P-value:4.26E-5|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:MOB1(YIL106W)|FD-Score:3.24|P-value:5.99E-4|Clearance:0.08||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:-3.21|P-value:6.72E-4|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:NUT2(YPR168W)|FD-Score:-3.15|P-value:8.21E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:PRP19(YLL036C)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.2||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPB3(YIL021W)|FD-Score:4.1|P-value:2.10E-5|Clearance:0.6||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RRP5(YMR229C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.17||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:RVB2(YPL235W)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.6||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:TAF3(YPL011C)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.6||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TIF11(YMR260C)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:YTM1(YOR272W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:CDC5(YMR001C)|FD-Score:-3.93|P-value:4.26E-5|Clearance:0||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:MOB1(YIL106W)|FD-Score:3.24|P-value:5.99E-4|Clearance:0.08||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:-3.21|P-value:6.72E-4|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:NUT2(YPR168W)|FD-Score:-3.15|P-value:8.21E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:PRP19(YLL036C)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.2||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPB3(YIL021W)|FD-Score:4.1|P-value:2.10E-5|Clearance:0.6||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RRP5(YMR229C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.17||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:RVB2(YPL235W)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.6||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:TAF3(YPL011C)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.6||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TIF11(YMR260C)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:YTM1(YOR272W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3598164
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM26(YKL037W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ARP8(YOR141C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ASN1(YPR145W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BBC1(YJL020C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:CWC27(YPL064C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DIT1(YDR403W)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DMA1(YHR115C)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS4(YPR017C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EAP1(YKL204W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM1(YAL059W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm Gene:GND2(YGR256W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HIM1(YDR317W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HUG1(YML058W-A)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:HXT4(YHR092C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:HYR1(YIR037W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ITC1(YGL133W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KIN2(YLR096W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LPP1(YDR503C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:MEH1(YKR007W)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MTF2(YDL044C)|FD-Score:4.36|P-value:6.65E-6||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:MTG1(YMR097C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NNF2(YGR089W)|FD-Score:4.39|P-value:5.59E-6||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUR1(YDL089W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OSH7(YHR001W)|FD-Score:-4|P-value:3.20E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PIM1(YBL022C)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:RAD1(YPL022W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RIM13(YMR154C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:ROT2(YBR229C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RRP8(YDR083W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SAS3(YBL052C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SBH1(YER087C-B)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC66(YBR171W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPO16(YHR153C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:VMA13(YPR036W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAP1801(YHR161C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCR099C(YCR099C_p)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function Gene:YDL050C(YDL050C_d)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR535C(YDR535C_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR174W-A(YGR174W-A_p)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YHP1(YDR451C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL108W(YIL108W_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIL161W(YIL161W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YIL168W(YIL168W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJR154W(YJR154W_p)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR047W(YKR047W_d)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR030W(YLR030W_p)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Putative protein of unknown function Gene:YNL195C(YNL195C_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL162W(YOL162W_p)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPR027C(YPR027C_p)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Putative protein of unknown function Gene:YPR147C(YPR147C_p)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YPS6(YIR039C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:ADK1(YDR226W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM26(YKL037W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ARP8(YOR141C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ASN1(YPR145W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BBC1(YJL020C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:CWC27(YPL064C)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DIT1(YDR403W)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DMA1(YHR115C)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS4(YPR017C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EAP1(YKL204W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM1(YAL059W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm Gene:GND2(YGR256W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HIM1(YDR317W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HUG1(YML058W-A)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:HXT4(YHR092C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:HYR1(YIR037W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ITC1(YGL133W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KIN2(YLR096W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LPP1(YDR503C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:MEH1(YKR007W)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MTF2(YDL044C)|FD-Score:4.36|P-value:6.65E-6||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:MTG1(YMR097C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NNF2(YGR089W)|FD-Score:4.39|P-value:5.59E-6||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUR1(YDL089W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OSH7(YHR001W)|FD-Score:-4|P-value:3.20E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PIM1(YBL022C)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:RAD1(YPL022W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RIM13(YMR154C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:ROT2(YBR229C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RRP8(YDR083W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SAS3(YBL052C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SBH1(YER087C-B)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC66(YBR171W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPO16(YHR153C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:VMA13(YPR036W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAP1801(YHR161C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCR099C(YCR099C_p)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function Gene:YDL050C(YDL050C_d)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR535C(YDR535C_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR174W-A(YGR174W-A_p)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YHP1(YDR451C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL108W(YIL108W_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIL161W(YIL161W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YIL168W(YIL168W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJR154W(YJR154W_p)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR047W(YKR047W_d)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR030W(YLR030W_p)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Putative protein of unknown function Gene:YNL195C(YNL195C_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL162W(YOL162W_p)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPR027C(YPR027C_p)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Putative protein of unknown function Gene:YPR147C(YPR147C_p)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YPS6(YIR039C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL235W4.231.16E-50.60RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YIL021W4.102.10E-50.60RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YPL011C4.042.68E-50.60TAF3TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
YMR229C3.442.89E-40.17RRP5RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
YOR272W3.275.31E-40.03YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YIL106W3.245.99E-40.08MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YLL036C3.167.87E-40.20PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YGL061C2.960.001550.09DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YML077W2.860.002090.02BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YDR355C_d2.850.002190.04YDR355C_dDubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
YLR378C2.810.002450.05SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YHR062C2.760.002890.01RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR487C2.750.003020.02RIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
YPL211W2.730.003190.05NIP7Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
YCL059C2.680.003730.07KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR089W4.395.59E-6NNF2Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
YDL044C4.366.65E-6MTF2Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
YPR017C4.201.35E-5DSS4Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
YPL064C4.052.56E-5CWC27Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
YIL168W4.012.97E-5YIL168WOpen reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase
YDR451C4.013.00E-5YHP1Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
YIR039C3.944.05E-5YPS6Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance
YBL022C3.894.97E-5PIM1ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
YGR174W-A_p3.816.97E-5YGR174W-A_pPutative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis
YKR007W3.807.33E-5MEH1Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
YHR115C3.787.76E-5DMA1Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger
YDL089W3.749.21E-5NUR1Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate
YJR154W_p3.739.75E-5YJR154W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YPR145W3.701.10E-4ASN1Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
YLR096W3.681.16E-4KIN2Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p

GO enrichment analysis for SGTC_1224
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0691.02E-7SGTC_28849058502 52.0 μMChembridge (Drug-like library)64715540.0769231
0.0596.07E-6SGTC_8360330-0104 1.4 μMChemDiv (Drug-like library)464948200.0508475
0.0561.95E-5SGTC_13161272-0050 65.9 μMChemDiv (Drug-like library)73403920.0491803
0.0533.98E-5SGTC_22176934799 96.1 μMChembridge (Fragment library)6756060.0322581TSC3-RPN4
0.0526.88E-5SGTC_1084voriconazole 435.0 nMNIH Clinical Collection716160.0657895azole & statin
0.0501.09E-4SGTC_23256158266 200.0 μMChembridge (Fragment library)36232400.0338983
0.0472.84E-4SGTC_12340326-0494 15.5 μMChemDiv (Drug-like library)15478130.078125
0.0472.99E-4SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.0689655azole & statin
0.0473.36E-4SGTC_530973-0023 40.0 μMChemDiv (Drug-like library)6747680.0793651RNA processing & uracil transport
0.0464.36E-4SGTC_1701st028638 40.4 μMTimTec (Natural product derivative library)34908780.0941176
0.0447.72E-4SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.0617284ERAD & cell cycle
0.0448.26E-4SGTC_1037k284-1091 78.7 μMChemDiv (Drug-like library)33347110.0615385
0.0439.16E-4SGTC_1770st043884 66.3 μMTimTec (Natural product derivative library)6701490.057971
0.0420.00139SGTC_29167875993 68.8 μMChembridge (Drug-like library)12992500.0833333
0.0420.00142SGTC_31769106582 49.5 μMChembridge (Drug-like library)170180270.0606061

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2852902385451.95 μM0.24137919798528Chembridge (Drug-like library)312.386144.26505
SGTC_2982901940425.97 μM0.23728822333232Chembridge (Drug-like library)316.3500233.98506
SGTC_13823002-2117298 μM0.2264151497314ChemDiv (Drug-like library)246.35122.7903
SGTC_930109-004559.23 μM0.20408288733ChemDiv (Drug-like library)210.23132.53413DNA damage response
SGTC_2343799071614.84 μM0.26456862Chembridge (Fragment library)238.284462.87213
SGTC_21525568786200 μM0.1886795399883Chembridge (Fragment library)226.23071.76424
SGTC_21605660466132.93 μM0.1739132859134Chembridge (Fragment library)209.672082.5242260S ribosome export
SGTC_15130337-055919 μM0.173077775073ChemDiv (Drug-like library)211.216063.03813
SGTC_3252810-4341181 μM0.166667774970ChemDiv (Drug-like library)252.311043.96213amide catabolism
SGTC_2084530607860.64 μM0.166667679340Chembridge (Fragment library)258.2990.79326