0124-0032

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1228
Screen concentration 52.5 μM
Source ChemDiv (Drug-like library)
PubChem CID
SMILES N#CC(=C1[CH-]C(=C(C#N)C#N)c2ccccc12)C#N
Standardized SMILES N#CC(=C1[CH-]C(=C(C#N)C#N)c2ccccc12)C#N
Molecular weight 241.227
ALogP 2.54
H-bond donor count 4
H-bond acceptor count 0
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.42
% growth inhibition (Hom. pool) 6.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for X1228
Download HIP data (tab-delimited text)  (excel)
Gene:FAS2(YPL231W)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.01||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GDI1(YER136W)|FD-Score:4.1|P-value:2.09E-5|Clearance:0.16||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:KEI1(YDR367W)|FD-Score:3.79|P-value:7.47E-5|Clearance:0.45||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LCB2(YDR062W)|FD-Score:4.61|P-value:2.04E-6|Clearance:0.51||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NIP1(YMR309C)|FD-Score:3.94|P-value:4.07E-5|Clearance:0.15||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.37||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:ORC4(YPR162C)|FD-Score:-3.39|P-value:3.44E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RIB3(YDR487C)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RRP5(YMR229C)|FD-Score:-3.9|P-value:4.75E-5|Clearance:0||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:SPC3(YLR066W)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.02||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:UTP10(YJL109C)|FD-Score:-4.27|P-value:9.69E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:FAS2(YPL231W)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.01||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GDI1(YER136W)|FD-Score:4.1|P-value:2.09E-5|Clearance:0.16||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:KEI1(YDR367W)|FD-Score:3.79|P-value:7.47E-5|Clearance:0.45||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LCB2(YDR062W)|FD-Score:4.61|P-value:2.04E-6|Clearance:0.51||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NIP1(YMR309C)|FD-Score:3.94|P-value:4.07E-5|Clearance:0.15||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.37||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:ORC4(YPR162C)|FD-Score:-3.39|P-value:3.44E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RIB3(YDR487C)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RRP5(YMR229C)|FD-Score:-3.9|P-value:4.75E-5|Clearance:0||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:SPC3(YLR066W)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.02||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:UTP10(YJL109C)|FD-Score:-4.27|P-value:9.69E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for X1228
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ARF1(YDL192W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARG7(YMR062C)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARG82(YDR173C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BUD28(YLR062C_d)|FD-Score:5.84|P-value:2.62E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CEX1(YOR112W)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLC1(YGR167W)|FD-Score:5.47|P-value:2.19E-8||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX10(YPL172C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CPR4(YCR069W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSI2(YOL007C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:CSM4(YPL200W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DAL2(YIR029W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DEP1(YAL013W)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DGR1(YNL130C-A_p)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DOA4(YDR069C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DPH5(YLR172C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EPS1(YIL005W)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:EXO5(YBR163W)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FRE8(YLR047C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FYV6(YNL133C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:HUR1(YGL168W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IES3(YLR052W)|FD-Score:5.58|P-value:1.21E-8||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IPK1(YDR315C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IPT1(YDR072C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KEX1(YGL203C)|FD-Score:5.81|P-value:3.18E-9||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:LCB5(YLR260W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MRPL10(YNL284C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MSB1(YOR188W)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSS2(YDL107W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:MTF1(YMR228W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:PCL5(YHR071W)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PEP7(YDR323C)|FD-Score:5.62|P-value:9.29E-9||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PIL1(YGR086C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:RDL1(YOR285W)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:REI1(YBR267W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RNR3(YIL066C)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPL7A(YGL076C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS29B(YDL061C)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:SAP190(YKR028W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SHM2(YLR058C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIM1(YIL123W)|FD-Score:-4.39|P-value:5.78E-6||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SLM6(YBR266C_d)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNF6(YHL025W)|FD-Score:-5.43|P-value:2.84E-8||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPT7(YBR081C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:STV1(YMR054W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUS1(YBR111W-A)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWF1(YDR126W)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TCM62(YBR044C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TRM9(YML014W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:VMA13(YPR036W)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Putative protein of unknown function Gene:YDR203W(YDR203W_d)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR391C(YDR391C_p)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR426C(YDR426C_d)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YER137C(YER137C_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Putative protein of unknown function Gene:YHC3(YJL059W)|FD-Score:3.74|P-value:9.08E-5||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKL077W(YKL077W_p)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKR011C(YKR011C_p)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YML122C(YML122C_d)|FD-Score:-3.13|P-value:8.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Putative protein of unknown function Gene:YOL038C-A(YOL038C-A_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YPL185W(YPL185W_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR022C(YPR022C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR130C(YPR130C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACO1(YLR304C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ARF1(YDL192W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARG7(YMR062C)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ARG82(YDR173C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BUD28(YLR062C_d)|FD-Score:5.84|P-value:2.62E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CEX1(YOR112W)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLC1(YGR167W)|FD-Score:5.47|P-value:2.19E-8||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX10(YPL172C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CPR4(YCR069W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSI2(YOL007C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:CSM4(YPL200W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DAL2(YIR029W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DEP1(YAL013W)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DGR1(YNL130C-A_p)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DOA4(YDR069C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DPH5(YLR172C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EPS1(YIL005W)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:EXO5(YBR163W)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FRE8(YLR047C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FYV6(YNL133C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:HUR1(YGL168W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IES3(YLR052W)|FD-Score:5.58|P-value:1.21E-8||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IPK1(YDR315C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IPT1(YDR072C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KEX1(YGL203C)|FD-Score:5.81|P-value:3.18E-9||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:LCB5(YLR260W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MRPL10(YNL284C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MSB1(YOR188W)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSS2(YDL107W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:MTF1(YMR228W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:PCL5(YHR071W)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PEP7(YDR323C)|FD-Score:5.62|P-value:9.29E-9||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PIL1(YGR086C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:RDL1(YOR285W)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:REI1(YBR267W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RNR3(YIL066C)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPL7A(YGL076C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS29B(YDL061C)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:SAP190(YKR028W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SHM2(YLR058C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIM1(YIL123W)|FD-Score:-4.39|P-value:5.78E-6||SGD DESC:Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated Gene:SLM6(YBR266C_d)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNF6(YHL025W)|FD-Score:-5.43|P-value:2.84E-8||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPT7(YBR081C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:STV1(YMR054W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUS1(YBR111W-A)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWF1(YDR126W)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TCM62(YBR044C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TRM9(YML014W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:VMA13(YPR036W)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Putative protein of unknown function Gene:YDR203W(YDR203W_d)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR391C(YDR391C_p)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR426C(YDR426C_d)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YER137C(YER137C_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Putative protein of unknown function Gene:YHC3(YJL059W)|FD-Score:3.74|P-value:9.08E-5||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKL077W(YKL077W_p)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKR011C(YKR011C_p)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YML122C(YML122C_d)|FD-Score:-3.13|P-value:8.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Putative protein of unknown function Gene:YOL038C-A(YOL038C-A_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YPL185W(YPL185W_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR022C(YPR022C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR130C(YPR130C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR062W4.612.04E-60.51LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YER136W4.102.09E-50.16GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YMR309C3.944.07E-50.15NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YDR367W3.797.47E-50.45KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YLR066W3.344.19E-40.02SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YPL231W3.324.46E-40.01FAS2Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
YHR170W3.324.58E-40.37NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR404C2.940.001630.03RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YLR002C2.910.001810.04NOC3Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
YLR340W2.870.002030.00RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YOL021C2.870.002050.13DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDR170C2.740.003050.05SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
YGL102C_d2.700.003500.07YGL102C_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
YPL210C2.630.004320.01SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YOR181W2.610.004470.03LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR062C_d5.842.62E-9BUD28_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YGL203C5.813.18E-9KEX1Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
YDR069C5.803.27E-9DOA4Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
YDR323C5.629.29E-9PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YLR052W5.581.21E-8IES3Subunit of the INO80 chromatin remodeling complex
YGR167W5.472.19E-8CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YDR126W5.032.40E-7SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YOR188W4.532.90E-6MSB1Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
YHR071W4.385.81E-6PCL5Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity
YIL066C4.376.11E-6RNR3Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits
YLR304C4.141.77E-5ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YDL061C4.082.25E-5RPS29BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
YPL172C4.072.34E-5COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YMR054W4.032.74E-5STV1Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
YLR172C4.022.86E-5DPH5Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm

GO enrichment analysis for SGTC_1228
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1032.07E-15SGTC_2615purpurogallin-4-carboxylic acid 100.0 μMMicrosource (Natural product library)2693150.0612245redox potentiating
0.1023.13E-15SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634300.05
0.0807.24E-10SGTC_2144373-3496 70.0 μMChemDiv (Drug-like library)37791660.0675676
0.0791.08E-9SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.05
0.0781.70E-9SGTC_340469-0683 81.8 μMChemDiv (Drug-like library)36909230.0555556SWF1 & branched chain AA biosynthesis
0.0781.81E-9SGTC_12660828-0227 32.9 μMChemDiv (Drug-like library)39138200.155556
0.0756.49E-9SGTC_20435101342 149.0 μMChembridge (Fragment library)6784510.12766SWF1 & branched chain AA biosynthesis
0.0732.35E-8SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0186335Golgi
0.0723.29E-8SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0810811
0.0723.40E-8SGTC_508roscovitine 141.0 μMICCB bioactive library1603550.0757576
0.0691.05E-7SGTC_1711st032263 62.8 μMTimTec (Natural product derivative library)39589790.0806452
0.0691.12E-7SGTC_20345197015 53.1 μMChembridge (Fragment library)7806760.117647SWF1 & branched chain AA biosynthesis
0.0691.14E-7SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.0434783
0.0691.19E-7SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.109091SWF1 & branched chain AA biosynthesis
0.0681.95E-7SGTC_12181348-1416 30.7 μMChemDiv (Drug-like library)67876680.140351SWF1 & branched chain AA biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20585236698133 μM0.238095766665Chembridge (Fragment library)214.263061.36503
SGTC_370566-00701.56 μM0.2307691626686ChemDiv (Drug-like library)189.233682.13203
SGTC_503tyrphostin 8294 μM0.22857192970ICCB bioactive library170.167441.77313TSC3-RPN4
SGTC_11554088-039448.2 μM0.2272732326248ChemDiv (Drug-like library)256.365943.70112
SGTC_1693xanthone101.9 μM0.218757020TimTec (Natural product derivative library)196.201422.96402DNA damage response
SGTC_2532xanthone78.9 μM0.218757020Microsource (Natural product library)196.201422.96402
SGTC_1923455-060960.9 μM0.2105263769639ChemDiv (Drug-like library)193.652763.52602
SGTC_1915st06710530.6 μM0.20588269751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_20865274770176.62 μM0.205128689278Chembridge (Fragment library)254.31031.46325
SGTC_90092-000210.45 μM0.2759368ChemDiv (Drug-like library)276.286082.92603
SGTC_1350933-000426.35 μM0.221230617ChemDiv (Drug-like library)235.240762.9721360S ribosome export