0325-0408

4-[(4-methylphenyl)diazenyl]aniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1233
Screen concentration 14.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 12874
SMILES CC1=CC=C(C=C1)N=NC2=CC=C(C=C2)N
Standardized SMILES Cc1ccc(cc1)N=Nc2ccc(N)cc2
Molecular weight 211.2624
ALogP 3.94
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.06
% growth inhibition (Hom. pool) 7.21


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 12874
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:4.83|P-value:6.90E-7|Clearance:0.34||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:CDC53(YDL132W)|FD-Score:-4.3|P-value:8.56E-6|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:ERD2(YBL040C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.26||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:LSM2(YBL026W)|FD-Score:4.48|P-value:3.65E-6|Clearance:0.31||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NIP1(YMR309C)|FD-Score:5.99|P-value:1.05E-9|Clearance:1.16||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP1(YDL014W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.19||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:POL5(YEL055C)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.11||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRE8(YML092C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.01||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RLP24(YLR009W)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.08||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RNA15(YGL044C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.19||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB11(YOL005C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.21||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPC19(YNL113W)|FD-Score:3.72|P-value:9.97E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPC37(YKR025W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.18||SGD DESC:RNA polymerase III subunit C37 Gene:SEN2(YLR105C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.06||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:TOM40(YMR203W)|FD-Score:-3.21|P-value:6.69E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP15(YMR093W)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ACP1(YKL192C)|FD-Score:4.83|P-value:6.90E-7|Clearance:0.34||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:CDC53(YDL132W)|FD-Score:-4.3|P-value:8.56E-6|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:ERD2(YBL040C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.26||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:LSM2(YBL026W)|FD-Score:4.48|P-value:3.65E-6|Clearance:0.31||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NIP1(YMR309C)|FD-Score:5.99|P-value:1.05E-9|Clearance:1.16||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP1(YDL014W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.19||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:POL5(YEL055C)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.11||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRE8(YML092C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.01||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RLP24(YLR009W)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.08||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RNA15(YGL044C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.19||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB11(YOL005C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.21||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPC19(YNL113W)|FD-Score:3.72|P-value:9.97E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPC37(YKR025W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.18||SGD DESC:RNA polymerase III subunit C37 Gene:SEN2(YLR105C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.06||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:TOM40(YMR203W)|FD-Score:-3.21|P-value:6.69E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP15(YMR093W)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 12874
Download HOP data (tab-delimited text)  (excel)
Gene:ALF1(YNL148C)|FD-Score:5.09|P-value:1.81E-7||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL2(YKL135C)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:BRP1(YGL007W_d)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CBS2(YDR197W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CKB1(YGL019W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLA4(YNL298W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLN3(YAL040C)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CST6(YIL036W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DJP1(YIR004W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:EAP1(YKL204W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM25(YJL201W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:FRD1(YEL047C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:4.84|P-value:6.34E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLG2(YJL137C)|FD-Score:-3.9|P-value:4.77E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HOM2(YDR158W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC10(YOL015W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC6(YFR043C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KIN2(YLR096W)|FD-Score:5.36|P-value:4.15E-8||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LTE1(YAL024C)|FD-Score:4.88|P-value:5.33E-7||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MDY2(YOL111C)|FD-Score:3.72|P-value:9.85E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MNR2(YKL064W)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MPC2(YHR162W)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MSH5(YDL154W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NNF2(YGR089W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUP170(YBL079W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PEX14(YGL153W)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PHO88(YBR106W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAV1(YJR033C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RGP1(YDR137W)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPN4(YDL020C)|FD-Score:7.85|P-value:2.10E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SNT1(YCR033W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPF1(YEL031W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO14(YKR031C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPS2(YDR522C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRO7(YPR032W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:6.4|P-value:7.61E-11||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TOF2(YKR010C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPO3(YPR156C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UIP3(YAR027W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:VMA13(YPR036W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:XPT1(YJR133W)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YBR013C(YBR013C_p)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR056W(YBR056W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR174C(YBR174C_d)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR238C(YBR238C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR064C(YCR064C_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR179W-A(YDR179W-A_p)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Putative protein of unknown function Gene:YDR336W(YDR336W_p)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YJL022W(YJL022W_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YMD8(YML038C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML122C(YML122C_d)|FD-Score:-5.7|P-value:6.06E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR082C(YOR082C_d)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPK1(YKL126W)|FD-Score:12.3|P-value:2.44E-35||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL136W(YPL136W_d)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR078C(YPR078C_p)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ALF1(YNL148C)|FD-Score:5.09|P-value:1.81E-7||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL2(YKL135C)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:BRP1(YGL007W_d)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CBS2(YDR197W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CKB1(YGL019W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLA4(YNL298W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLN3(YAL040C)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CST6(YIL036W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DJP1(YIR004W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:EAP1(YKL204W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM25(YJL201W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:FRD1(YEL047C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:4.84|P-value:6.34E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLG2(YJL137C)|FD-Score:-3.9|P-value:4.77E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HOM2(YDR158W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC10(YOL015W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC6(YFR043C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KIN2(YLR096W)|FD-Score:5.36|P-value:4.15E-8||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LTE1(YAL024C)|FD-Score:4.88|P-value:5.33E-7||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MDY2(YOL111C)|FD-Score:3.72|P-value:9.85E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MNR2(YKL064W)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MPC2(YHR162W)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MSH5(YDL154W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NNF2(YGR089W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUP170(YBL079W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PEX14(YGL153W)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PHO88(YBR106W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAV1(YJR033C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RGP1(YDR137W)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPN4(YDL020C)|FD-Score:7.85|P-value:2.10E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SNT1(YCR033W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPF1(YEL031W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO14(YKR031C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPS2(YDR522C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRO7(YPR032W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:6.4|P-value:7.61E-11||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TOF2(YKR010C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPO3(YPR156C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UIP3(YAR027W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:VMA13(YPR036W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:XPT1(YJR133W)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YBR013C(YBR013C_p)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR056W(YBR056W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR174C(YBR174C_d)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR238C(YBR238C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR064C(YCR064C_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR179W-A(YDR179W-A_p)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Putative protein of unknown function Gene:YDR336W(YDR336W_p)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YJL022W(YJL022W_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YMD8(YML038C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML122C(YML122C_d)|FD-Score:-5.7|P-value:6.06E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR082C(YOR082C_d)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPK1(YKL126W)|FD-Score:12.3|P-value:2.44E-35||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL136W(YPL136W_d)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR078C(YPR078C_p)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR309C5.991.05E-91.16NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YKL192C4.836.90E-70.34ACP1Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
YBL026W4.483.65E-60.31LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YGL044C4.181.48E-50.19RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YDL014W3.983.39E-50.18NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YLR009W3.807.27E-50.08RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YNL113W3.729.97E-50.03RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YML092C3.691.12E-40.01PRE8Alpha 2 subunit of the 20S proteasome
YLR105C3.681.18E-40.06SEN2Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
YOL005C3.621.48E-40.21RPB11RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
YKR025W3.413.28E-40.18RPC37RNA polymerase III subunit C37
YEL055C3.236.28E-40.11POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YBL040C3.119.29E-40.26ERD2HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
YFR050C2.850.002160.06PRE4Beta 7 subunit of the 20S proteasome
YOR250C2.790.002600.00CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W12.302.44E-35YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDL020C7.852.10E-15RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YOR047C6.407.61E-11STD1Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]
YGL007W_d5.581.18E-8BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YDR137W5.383.82E-8RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YLR096W5.364.15E-8KIN2Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p
YNL148C5.091.81E-7ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YAL024C4.885.33E-7LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YHR100C4.846.34E-7GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YKL135C4.711.27E-6APL2Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
YOR082C_d4.641.78E-6YOR082C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W
YEL031W4.631.82E-6SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YDL154W4.621.88E-6MSH5Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
YJR133W4.386.05E-6XPT1Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine
YFR043C4.221.24E-5IRC6Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci

GO enrichment analysis for SGTC_1233
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2144.21E-62SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.175
0.1939.38E-51SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0434783
0.1653.28E-37SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.0508475
0.1591.01E-34SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.056338
0.1571.13E-33SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0892857ERG2
0.1549.55E-33SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0666667
0.1511.33E-31SGTC_5941487-1584 10.8 μMChemDiv (Drug-like library)50133320.05
0.1478.48E-30SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0666667
0.1433.34E-28SGTC_21575649910 96.4 μMChembridge (Fragment library)28580910.0909091
0.1425.06E-28SGTC_2740felodipine 17.1 μMMiscellaneous33330.0877193
0.1366.94E-26SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.0819672
0.1345.88E-25SGTC_29187977171 22.1 μMChembridge (Drug-like library)22364830.0714286
0.1323.50E-24SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0655738calcium & mitochondrial duress
0.1314.38E-24SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.0666667
0.1286.69E-23SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.102041fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1169aniline yellow25.8 μM0.6086966051ChemDiv (Drug-like library)197.235843.45213
SGTC_7690302-023176 μM0.571429571869ChemDiv (Drug-like library)225.2894.42513
SGTC_6140462-0418107 μM0.3513515188303ChemDiv (Drug-like library)256.303043.17625
SGTC_21605660466132.93 μM0.3225812859134Chembridge (Fragment library)209.672082.5242260S ribosome export
SGTC_2236dapsone200 μM0.2962962955Miscellaneous248.300881.43924
SGTC_9242046-004421.3 μM0.2820513623589ChemDiv (Drug-like library)312.40765.83202
SGTC_21826325192200 μM0.282051736248Chembridge (Fragment library)226.273762.35422Golgi
SGTC_23859074692200 μM0.2812517046906Chembridge (Fragment library)201.693142.6422
SGTC_8370091-026512.5 μM0.266667759367ChemDiv (Drug-like library)264.11793.39212ubiquinone biosynthesis & proteasome
SGTC_6690108-002126.2 μM0.25806517201ChemDiv (Drug-like library)292.331843.45724fatty acid desaturase (OLE1)