0330-0013

1-ethyl-4-hydroxy-4,6,6-trimethyl-3-phenyl-1,3-diazinan-2-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1236
Screen concentration 258.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4617066
SMILES CCN1C(=O)N(C(CC1(C)C)(C)O)C2=CC=CC=C2
Standardized SMILES CCN1C(=O)N(c2ccccc2)C(C)(O)CC1(C)C
Molecular weight 262.3474
ALogP 1.96
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.83
% growth inhibition (Hom. pool) 4.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4617066
Download HIP data (tab-delimited text)  (excel)
Gene:CDS1(YBR029C)|FD-Score:-3.3|P-value:4.80E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:GPI12(YMR281W)|FD-Score:3.79|P-value:7.55E-5|Clearance:0.53||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:HEM3(YDL205C)|FD-Score:-3.11|P-value:9.21E-4|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HRR25(YPL204W)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.53||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:NIP1(YMR309C)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.53||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:PDC2(YDR081C)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.53||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PDI1(YCL043C)|FD-Score:3.83|P-value:6.32E-5|Clearance:0.53||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:RLP7(YNL002C)|FD-Score:-3.89|P-value:5.01E-5|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:SUI3(YPL237W)|FD-Score:3.55|P-value:1.89E-4|Clearance:0.53||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TSR4(YOL022C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.53||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:YPI1(YFR003C)|FD-Score:-5.29|P-value:6.26E-8|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:CDS1(YBR029C)|FD-Score:-3.3|P-value:4.80E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:GPI12(YMR281W)|FD-Score:3.79|P-value:7.55E-5|Clearance:0.53||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:HEM3(YDL205C)|FD-Score:-3.11|P-value:9.21E-4|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HRR25(YPL204W)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.53||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:NIP1(YMR309C)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.53||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:PDC2(YDR081C)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.53||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PDI1(YCL043C)|FD-Score:3.83|P-value:6.32E-5|Clearance:0.53||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:RLP7(YNL002C)|FD-Score:-3.89|P-value:5.01E-5|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:SUI3(YPL237W)|FD-Score:3.55|P-value:1.89E-4|Clearance:0.53||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TSR4(YOL022C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.53||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:YPI1(YFR003C)|FD-Score:-5.29|P-value:6.26E-8|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4617066
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AIM22(YJL046W)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ANP1(YEL036C)|FD-Score:7.68|P-value:7.77E-15||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:CBP1(YJL209W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:DPH2(YKL191W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p Gene:FMP33(YJL161W_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HSC82(YMR186W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:MIP1(YOR330C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MKC7(YDR144C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MPM1(YJL066C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRH4(YGL064C)|FD-Score:-4.17|P-value:1.49E-5||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MTF2(YDL044C)|FD-Score:-4.64|P-value:1.75E-6||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NGL2(YMR285C)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NPC2(YDL046W)|FD-Score:-3.82|P-value:6.72E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:NRK1(YNL129W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis Gene:NTH1(YDR001C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:NYV1(YLR093C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OPI11(YPR044C_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PES4(YFR023W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET112(YBL080C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX11(YOL147C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PEX19(YDL065C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PMP1(YCR024C-A)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PMT7(YDR307W_p)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PRK1(YIL095W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:RIM13(YMR154C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM4(YHL024W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:SAC6(YDR129C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDL1(YIL167W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SEH1(YGL100W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SPA2(YLL021W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:STB4(YMR019W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:TPM2(YIL138C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TSC3(YBR058C-A)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UIP4(YPL186C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA1(YDL185W)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS45(YGL095C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS55(YJR044C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBR219C(YBR219C_p)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.73|P-value:9.40E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YIL055C(YIL055C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function Gene:YKL023W(YKL023W_p)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YMD8(YML038C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR114C(YMR114C)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YPL185W(YPL185W_d)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:AAT2(YLR027C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AIM22(YJL046W)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ANP1(YEL036C)|FD-Score:7.68|P-value:7.77E-15||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:CBP1(YJL209W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:DPH2(YKL191W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p Gene:FMP33(YJL161W_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HSC82(YMR186W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:MIP1(YOR330C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MKC7(YDR144C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MPM1(YJL066C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRH4(YGL064C)|FD-Score:-4.17|P-value:1.49E-5||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MTF2(YDL044C)|FD-Score:-4.64|P-value:1.75E-6||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NGL2(YMR285C)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NPC2(YDL046W)|FD-Score:-3.82|P-value:6.72E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:NRK1(YNL129W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis Gene:NTH1(YDR001C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:NYV1(YLR093C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OPI11(YPR044C_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PES4(YFR023W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET112(YBL080C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX11(YOL147C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PEX19(YDL065C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PMP1(YCR024C-A)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PMT7(YDR307W_p)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PRK1(YIL095W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:RIM13(YMR154C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM4(YHL024W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:SAC6(YDR129C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDL1(YIL167W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SEH1(YGL100W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SPA2(YLL021W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:STB4(YMR019W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:TPM2(YIL138C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TSC3(YBR058C-A)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UIP4(YPL186C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA1(YDL185W)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS45(YGL095C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS55(YJR044C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBR219C(YBR219C_p)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.73|P-value:9.40E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YIL055C(YIL055C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function Gene:YKL023W(YKL023W_p)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YMD8(YML038C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR114C(YMR114C)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YPL185W(YPL185W_d)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR309C3.963.80E-50.53NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YCL043C3.836.32E-50.53PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YMR281W3.797.55E-50.53GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YPL204W3.758.80E-50.53HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YPL237W3.551.89E-40.53SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YOL022C3.393.45E-40.53TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
YDR081C3.314.64E-40.53PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YDL220C2.790.002670.15CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
YHR068W2.630.004270.13DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YDR327W_d2.500.006140.06YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
YDR232W2.440.007300.00HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YLR051C2.440.007349.70E-4FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YHR019C2.440.007360.03DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YGR082W2.410.008020.00TOM20Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
YGR119C2.410.008060.05NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL036C7.687.77E-15ANP1Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
YKL023W_p5.161.25E-7YKL023W_pPutative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YMR019W4.651.69E-6STB4Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
YOL147C4.425.02E-6PEX11Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p
YLR027C4.181.46E-5AAT2Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
YML038C3.944.00E-5YMD8Putative nucleotide sugar transporter, has similarity to Vrg4p
YDL185W3.944.11E-5VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YIL167W3.895.00E-5SDL1Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase
YDL065C3.865.60E-5PEX19Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
YFR023W3.846.13E-5PES4Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
YMR114C3.836.31E-5YMR114CProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
YMR285C3.836.43E-5NGL2Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
YPL185W_d3.778.23E-5YPL185W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C
YDR034W-B_p3.739.40E-5YDR034W-B_pPredicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YBL080C3.739.47E-5PET112Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog

GO enrichment analysis for SGTC_1236
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1111.29E-17SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.148148heat shock/prefoldin
0.1071.21E-16SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0483871TSC3-RPN4
0.1001.16E-14SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.09375TSC3-RPN4
0.0936.96E-13SGTC_524r073-0010 27.8 μMChemDiv (Drug-like library)27721060.0746269
0.0921.47E-12SGTC_2405hr heat shock (37°C) + Streptovitacin 300.0 μMMiscellaneous914670.16129heat shock/prefoldin
0.0921.55E-12SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.171429TSC3-RPN4
0.0898.76E-12SGTC_2395hr heat shock (37°C) + mitomycin C 819.4 μMMiscellaneous57460.103896heat shock/prefoldin
0.0862.85E-11SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0735294TSC3-RPN4
0.0841.28E-10SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.119403TSC3-RPN4
0.0814.21E-10SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.112676TSC3-RPN4
0.0814.58E-10SGTC_12220366-0074 1.2 μMChemDiv (Drug-like library)2865200.185185TSC3-RPN4
0.0809.17E-10SGTC_2740felodipine 17.1 μMMiscellaneous33330.115942
0.0791.07E-9SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0983607TSC3-RPN4
0.0791.24E-9SGTC_2425hr heat shock (37°C) + 5-fluorouracil 4.6 μMMiscellaneous33850.0816327heat shock/prefoldin
0.0772.52E-9SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0645161TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_511blebbistatin171 μM0.2622953476986ICCB bioactive library292.331842.42613DNA intercalators
SGTC_20985376439882.5 nM0.252844116Chembridge (Fragment library)255.268621.04903
SGTC_5643555-0129330 μM0.241379563260ChemDiv (Drug-like library)297.414563.66803
SGTC_2147554593289.72 μM0.24797404Chembridge (Fragment library)232.32142.33802ergosterol biosynthesis
SGTC_20505263056171 μM0.235294763468Chembridge (Fragment library)268.35353.29113fatty acid desaturase (OLE1)
SGTC_2474532157043.32 μM0.2321432841384Miscellaneous297.37153.55303
SGTC_5891227-007034.1 μM0.2321432841384ChemDiv (Drug-like library)297.37153.55303
SGTC_9113456-4043236 μM0.229508914281ChemDiv (Drug-like library)280.321143.06312mitochondrial processes
SGTC_1101ebselen5.29 μM0.2291673194NIH Clinical Collection274.176663.22701
SGTC_21215257820149.64 μM0.229167791256Chembridge (Fragment library)204.225180.85313