0136-0217

[4-chloro-2-[(2-chloro-4-nitrophenyl)carbamoyl]phenyl] 4-nitrobenzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_124
Screen concentration 7.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 3976905
SMILES C1=CC(=CC=C1C(=O)OC2=C(C=C(C=C2)Cl)C(=O)NC3=C(C=C(C=C3)[N+](=O)[O-])Cl)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(cc1)C(=O)Oc2ccc(Cl)cc2C(=O)Nc3ccc(cc3Cl)[N+](=O)[O-]
Molecular weight 476.2232
ALogP 5.16
H-bond donor count 1
H-bond acceptor count 7
Response signature mitochondrial stress

Pool Growth Kinetics
% growth inhibition (Het. pool) 53.51
% growth inhibition (Hom. pool) 18.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3976905
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:3.33|P-value:4.38E-4|Clearance:0.01||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:CAB1(YDR531W)|FD-Score:5.74|P-value:4.74E-9|Clearance:0.14||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CCT3(YJL014W)|FD-Score:6.42|P-value:6.66E-11|Clearance:0.37||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:-3.13|P-value:8.86E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC24(YLR323C)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.08||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DIM1(YPL266W)|FD-Score:5.75|P-value:4.55E-9|Clearance:0.01||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:6.83|P-value:4.13E-12|Clearance:0.41||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DUT1(YBR252W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.03||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GCD11(YER025W)|FD-Score:-5.1|P-value:1.71E-7|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HRT1(YOL133W)|FD-Score:4.4|P-value:5.37E-6|Clearance:0.08||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:ILV5(YLR355C)|FD-Score:4.21|P-value:1.30E-5|Clearance:0.04||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:LSM3(YLR438C-A)|FD-Score:5.22|P-value:9.00E-8|Clearance:0.14||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:LST8(YNL006W)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.16||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MCM1(YMR043W)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.13||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MED6(YHR058C)|FD-Score:3.87|P-value:5.46E-5|Clearance:0.1||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MEX67(YPL169C)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MGE1(YOR232W)|FD-Score:8.76|P-value:9.62E-19|Clearance:1.6||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:MIA40(YKL195W)|FD-Score:7.16|P-value:4.11E-13|Clearance:0.32||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MNP1(YGL068W)|FD-Score:5.08|P-value:1.87E-7|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NIC96(YFR002W)|FD-Score:-3.78|P-value:7.71E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP56(YLR197W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.08||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:9|P-value:1.09E-19|Clearance:0.24||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP85(YJR042W)|FD-Score:-3.18|P-value:7.35E-4|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PDS5(YMR076C)|FD-Score:4.92|P-value:4.31E-7|Clearance:0.12||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PMA1(YGL008C)|FD-Score:4.8|P-value:7.95E-7|Clearance:0.22||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RER2(YBR002C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIM2(YBR192W)|FD-Score:5.93|P-value:1.51E-9|Clearance:0.18||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:ROK1(YGL171W)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.06||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPC82(YPR190C)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPF1(YHR088W)|FD-Score:5.6|P-value:1.06E-8|Clearance:0.38||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL32(YBL092W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPM2(YML091C)|FD-Score:6.05|P-value:7.19E-10|Clearance:0.12||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPT4(YOR259C)|FD-Score:-3.97|P-value:3.59E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRP43(YCR035C)|FD-Score:4|P-value:3.18E-5|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SME1(YOR159C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.15||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:SNP1(YIL061C)|FD-Score:3.77|P-value:8.10E-5|Clearance:0.08||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPT5(YML010W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.02||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SQT1(YIR012W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.07||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SUP35(YDR172W)|FD-Score:12.1|P-value:8.90E-34|Clearance:3.05||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TAO3(YIL129C)|FD-Score:4.02|P-value:2.94E-5|Clearance:0.02||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TCP1(YDR212W)|FD-Score:4.32|P-value:7.91E-6|Clearance:0.11||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOM40(YMR203W)|FD-Score:5.06|P-value:2.06E-7|Clearance:0.14||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDR396W(YDR396W_d)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YJL086C(YJL086C_d)|FD-Score:3.12|P-value:9.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:4.5|P-value:3.33E-6|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YOL134C(YOL134C_d)|FD-Score:3.11|P-value:9.31E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:ABD1(YBR236C)|FD-Score:3.33|P-value:4.38E-4|Clearance:0.01||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:CAB1(YDR531W)|FD-Score:5.74|P-value:4.74E-9|Clearance:0.14||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CCT3(YJL014W)|FD-Score:6.42|P-value:6.66E-11|Clearance:0.37||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:-3.13|P-value:8.86E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC24(YLR323C)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.08||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DIM1(YPL266W)|FD-Score:5.75|P-value:4.55E-9|Clearance:0.01||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:6.83|P-value:4.13E-12|Clearance:0.41||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DUT1(YBR252W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.03||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GCD11(YER025W)|FD-Score:-5.1|P-value:1.71E-7|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HRT1(YOL133W)|FD-Score:4.4|P-value:5.37E-6|Clearance:0.08||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:ILV5(YLR355C)|FD-Score:4.21|P-value:1.30E-5|Clearance:0.04||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:LSM3(YLR438C-A)|FD-Score:5.22|P-value:9.00E-8|Clearance:0.14||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:LST8(YNL006W)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.16||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MCM1(YMR043W)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.13||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MED6(YHR058C)|FD-Score:3.87|P-value:5.46E-5|Clearance:0.1||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MEX67(YPL169C)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MGE1(YOR232W)|FD-Score:8.76|P-value:9.62E-19|Clearance:1.6||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:MIA40(YKL195W)|FD-Score:7.16|P-value:4.11E-13|Clearance:0.32||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MNP1(YGL068W)|FD-Score:5.08|P-value:1.87E-7|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NIC96(YFR002W)|FD-Score:-3.78|P-value:7.71E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP56(YLR197W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.08||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:9|P-value:1.09E-19|Clearance:0.24||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP85(YJR042W)|FD-Score:-3.18|P-value:7.35E-4|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PDS5(YMR076C)|FD-Score:4.92|P-value:4.31E-7|Clearance:0.12||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PMA1(YGL008C)|FD-Score:4.8|P-value:7.95E-7|Clearance:0.22||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RER2(YBR002C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIM2(YBR192W)|FD-Score:5.93|P-value:1.51E-9|Clearance:0.18||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:ROK1(YGL171W)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.06||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPC82(YPR190C)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPF1(YHR088W)|FD-Score:5.6|P-value:1.06E-8|Clearance:0.38||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL32(YBL092W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPM2(YML091C)|FD-Score:6.05|P-value:7.19E-10|Clearance:0.12||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPT4(YOR259C)|FD-Score:-3.97|P-value:3.59E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRP43(YCR035C)|FD-Score:4|P-value:3.18E-5|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SME1(YOR159C)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.15||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:SNP1(YIL061C)|FD-Score:3.77|P-value:8.10E-5|Clearance:0.08||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPT5(YML010W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.02||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SQT1(YIR012W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.07||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SUP35(YDR172W)|FD-Score:12.1|P-value:8.90E-34|Clearance:3.05||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TAO3(YIL129C)|FD-Score:4.02|P-value:2.94E-5|Clearance:0.02||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TCP1(YDR212W)|FD-Score:4.32|P-value:7.91E-6|Clearance:0.11||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOM40(YMR203W)|FD-Score:5.06|P-value:2.06E-7|Clearance:0.14||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDR396W(YDR396W_d)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YJL086C(YJL086C_d)|FD-Score:3.12|P-value:9.19E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:4.5|P-value:3.33E-6|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YOL134C(YOL134C_d)|FD-Score:3.11|P-value:9.31E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3976905
Download HOP data (tab-delimited text)  (excel)
Gene:AFT2(YPL202C)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AGE2(YIL044C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ARF1(YDL192W)|FD-Score:8.44|P-value:1.59E-17||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:5.08|P-value:1.91E-7||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BOI2(YER114C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BRE5(YNR051C)|FD-Score:6.86|P-value:3.42E-12||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BRO1(YPL084W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BTS1(YPL069C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD14(YAR014C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CBF1(YJR060W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:5.15|P-value:1.27E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:5.39|P-value:3.44E-8||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNB1(YKL190W)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:6.4|P-value:7.69E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DFG5(YMR238W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DID4(YKL002W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:EEB1(YPL095C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ERD1(YDR414C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERV14(YGL054C)|FD-Score:6.93|P-value:2.10E-12||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMO1(YHR176W)|FD-Score:4.79|P-value:8.37E-7||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FPR1(YNL135C)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GCN20(YFR009W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCS1(YDL226C)|FD-Score:8.35|P-value:3.40E-17||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIP3(YPL137C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GOS1(YHL031C)|FD-Score:6|P-value:9.77E-10||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GTT1(YIR038C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:GYP1(YOR070C)|FD-Score:18.7|P-value:2.64E-78||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HES1(YOR237W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:ILM1(YJR118C)|FD-Score:5.64|P-value:8.42E-9||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INP53(YOR109W)|FD-Score:14.4|P-value:1.29E-47||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:IRC24(YIR036C_p)|FD-Score:-4.37|P-value:6.11E-6||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:13.3|P-value:1.48E-40||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KRE1(YNL322C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KRE2(YDR483W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAK10(YEL053C)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAK3(YPR051W)|FD-Score:8.34|P-value:3.58E-17||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MNN11(YJL183W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRP7(YNL005C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSH2(YOL090W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NOT3(YIL038C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPR3(YHL023C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PDR3(YBL005W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:9.16|P-value:2.50E-20||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:7.28|P-value:1.61E-13||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PET123(YOR158W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PKR1(YMR123W)|FD-Score:5.25|P-value:7.71E-8||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMP1(YCR024C-A)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PSY4(YBL046W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:PTC1(YDL006W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RCY1(YJL204C)|FD-Score:6.78|P-value:6.11E-12||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM1(YCR028C-A)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RMD5(YDR255C)|FD-Score:-4.45|P-value:4.24E-6||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:ROT2(YBR229C)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPN4(YDL020C)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RUD3(YOR216C)|FD-Score:8|P-value:6.39E-16||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC6(YDR129C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:6.18|P-value:3.15E-10||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SFB3(YHR098C)|FD-Score:7.09|P-value:6.48E-13||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHE4(YOR035C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIN4(YNL236W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKY1(YMR216C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLM4(YBR077C)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SLM5(YCR024C)|FD-Score:5.59|P-value:1.11E-8||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLT2(YHR030C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SSO1(YPL232W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWA2(YDR320C)|FD-Score:4.54|P-value:2.75E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWF1(YDR126W)|FD-Score:4.94|P-value:3.81E-7||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SYS1(YJL004C)|FD-Score:7.58|P-value:1.67E-14||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TED1(YIL039W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TMA7(YLR262C-A)|FD-Score:3.76|P-value:8.44E-5||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRP5(YGL026C)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS85(YDR108W)|FD-Score:6.96|P-value:1.74E-12||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP3(YER151C)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBX5(YDR330W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:VAM10(YOR068C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS1(YKR001C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS13(YLL040C)|FD-Score:13.1|P-value:2.84E-39||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS17(YOR132W)|FD-Score:5.43|P-value:2.75E-8||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS21(YOR089C)|FD-Score:7.22|P-value:2.58E-13||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS24(YKL041W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS3(YDR495C)|FD-Score:6.25|P-value:2.08E-10||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:7.14|P-value:4.62E-13||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS36(YLR417W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS5(YOR069W)|FD-Score:4.92|P-value:4.40E-7||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS61(YDR136C_d)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:4.79|P-value:8.46E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:VPS9(YML097C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDL041W(YDL041W_d)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR008C(YDR008C_d)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Putative protein of unknown function Gene:YMR010W(YMR010W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR155W(YMR155W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YPL041C(YPL041C_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPR050C(YPR050C_d)|FD-Score:12.5|P-value:2.10E-36||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT6(YLR262C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRR1(YOR162C)|FD-Score:7.4|P-value:6.92E-14||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRT3(YKL175W)|FD-Score:4.37|P-value:6.33E-6||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency Gene:AFT2(YPL202C)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AGE2(YIL044C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ARF1(YDL192W)|FD-Score:8.44|P-value:1.59E-17||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:5.08|P-value:1.91E-7||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BOI2(YER114C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BRE5(YNR051C)|FD-Score:6.86|P-value:3.42E-12||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BRO1(YPL084W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BTS1(YPL069C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD14(YAR014C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CBF1(YJR060W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:5.15|P-value:1.27E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:5.39|P-value:3.44E-8||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNB1(YKL190W)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:6.4|P-value:7.69E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DFG5(YMR238W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DID4(YKL002W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:EEB1(YPL095C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ERD1(YDR414C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERV14(YGL054C)|FD-Score:6.93|P-value:2.10E-12||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMO1(YHR176W)|FD-Score:4.79|P-value:8.37E-7||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FPR1(YNL135C)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GCN20(YFR009W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCS1(YDL226C)|FD-Score:8.35|P-value:3.40E-17||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIP3(YPL137C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GOS1(YHL031C)|FD-Score:6|P-value:9.77E-10||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GTT1(YIR038C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:GYP1(YOR070C)|FD-Score:18.7|P-value:2.64E-78||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HES1(YOR237W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:ILM1(YJR118C)|FD-Score:5.64|P-value:8.42E-9||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INP53(YOR109W)|FD-Score:14.4|P-value:1.29E-47||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:IRC24(YIR036C_p)|FD-Score:-4.37|P-value:6.11E-6||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:13.3|P-value:1.48E-40||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KRE1(YNL322C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KRE2(YDR483W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAK10(YEL053C)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAK3(YPR051W)|FD-Score:8.34|P-value:3.58E-17||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MNN11(YJL183W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRP7(YNL005C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSH2(YOL090W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NOT3(YIL038C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPR3(YHL023C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PDR3(YBL005W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:9.16|P-value:2.50E-20||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:7.28|P-value:1.61E-13||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PET123(YOR158W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PKR1(YMR123W)|FD-Score:5.25|P-value:7.71E-8||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMP1(YCR024C-A)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PSY4(YBL046W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:PTC1(YDL006W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RCY1(YJL204C)|FD-Score:6.78|P-value:6.11E-12||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM1(YCR028C-A)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RMD5(YDR255C)|FD-Score:-4.45|P-value:4.24E-6||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:ROT2(YBR229C)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPN4(YDL020C)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RUD3(YOR216C)|FD-Score:8|P-value:6.39E-16||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC6(YDR129C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:6.18|P-value:3.15E-10||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SFB3(YHR098C)|FD-Score:7.09|P-value:6.48E-13||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHE4(YOR035C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIN4(YNL236W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKY1(YMR216C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLM4(YBR077C)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SLM5(YCR024C)|FD-Score:5.59|P-value:1.11E-8||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLT2(YHR030C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SSO1(YPL232W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWA2(YDR320C)|FD-Score:4.54|P-value:2.75E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWF1(YDR126W)|FD-Score:4.94|P-value:3.81E-7||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SYS1(YJL004C)|FD-Score:7.58|P-value:1.67E-14||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TED1(YIL039W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TMA7(YLR262C-A)|FD-Score:3.76|P-value:8.44E-5||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRP5(YGL026C)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS85(YDR108W)|FD-Score:6.96|P-value:1.74E-12||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP3(YER151C)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBX5(YDR330W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:VAM10(YOR068C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS1(YKR001C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS13(YLL040C)|FD-Score:13.1|P-value:2.84E-39||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS17(YOR132W)|FD-Score:5.43|P-value:2.75E-8||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS21(YOR089C)|FD-Score:7.22|P-value:2.58E-13||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS24(YKL041W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS3(YDR495C)|FD-Score:6.25|P-value:2.08E-10||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:7.14|P-value:4.62E-13||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS36(YLR417W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS5(YOR069W)|FD-Score:4.92|P-value:4.40E-7||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS61(YDR136C_d)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:4.79|P-value:8.46E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:VPS9(YML097C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDL041W(YDL041W_d)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR008C(YDR008C_d)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Putative protein of unknown function Gene:YMR010W(YMR010W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR155W(YMR155W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YPL041C(YPL041C_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPR050C(YPR050C_d)|FD-Score:12.5|P-value:2.10E-36||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT6(YLR262C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRR1(YOR162C)|FD-Score:7.4|P-value:6.92E-14||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRT3(YKL175W)|FD-Score:4.37|P-value:6.33E-6||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR172W12.108.90E-343.05SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YOL144W9.001.09E-190.24NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YOR232W8.769.62E-191.60MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YKL195W7.164.11E-130.32MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YMR211W6.834.13E-120.41DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YJL014W6.426.66E-110.37CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YML091C6.057.19E-100.12RPM2Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
YBR192W5.931.51E-90.18RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YPL266W5.754.55E-90.01DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YDR531W5.744.74E-90.14CAB1Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
YHR088W5.601.06E-80.38RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YLR438C-A5.229.00E-80.14LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YGL068W5.081.87E-70.02MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YMR203W5.062.06E-70.14TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YMR076C4.924.31E-70.12PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR070C18.702.64E-78GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YOR109W14.401.29E-47INP53Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
YKR019C13.301.48E-40IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YLL040C13.102.84E-39VPS13Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
YPR050C_d12.502.10E-36YPR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
YOR036W9.162.50E-20PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YDL192W8.441.59E-17ARF1ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
YDL226C8.353.40E-17GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YPR051W8.343.58E-17MAK3Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus
YOR216C8.006.39E-16RUD3Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
YJL004C7.581.67E-14SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YOR162C7.406.92E-14YRR1Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
YDR323C7.281.61E-13PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YOR089C7.222.58E-13VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YJL154C7.144.62E-13VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p

GO enrichment analysis for SGTC_124
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5160SGTC_1190121-0043 24.8 μMChemDiv (Drug-like library)10923780.235294mitochondrial stress
0.3151.83E-136SGTC_700141-0289 43.3 μMChemDiv (Drug-like library)3524080.246154mitochondrial stress
0.2958.94E-119SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.09375redox potentiating
0.2581.23E-90SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0394737Golgi
0.2462.72E-82SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.043956Golgi
0.2165.32E-63SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.0684932Golgi
0.2055.14E-57SGTC_459nigericin 15.3 μMICCB bioactive library342300.0322581
0.1992.36E-53SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.25
0.1865.33E-47SGTC_7680384-0033 77.3 μMChemDiv (Drug-like library)39653050.274194fatty acid desaturase (OLE1)
0.1851.96E-46SGTC_5283492-0019 172.0 μMChemDiv (Drug-like library)28692620.0352941calcium & mitochondrial duress
0.1797.80E-44SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.0543478
0.1782.74E-43SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.021978Golgi
0.1777.36E-43SGTC_232clozapine 119.0 μMMiscellaneous28180.113924NEO1
0.1742.39E-41SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634300.0314961
0.1689.76E-39SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.0689655NEO1-PIK1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3650121-005213.6 μM0.4655173101739ChemDiv (Drug-like library)361.564684.2624mitochondrial response to ROS
SGTC_7251497-096319 μM0.4642863549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress
SGTC_12510490-5699265 μM0.389833883818ChemDiv (Drug-like library)301.555783.66612
SGTC_7600568-072582.9 μM0.3833333916171ChemDiv (Drug-like library)380.010345.16613
SGTC_14780453-065635.7 μM0.3770495258496ChemDiv (Drug-like library)347.749762.81415
SGTC_1828st05581149 μM0.3571435877589TimTec (Natural product derivative library)407.803265.69205
SGTC_990109-0120622.8 μM0.344828759414ChemDiv (Drug-like library)321.126163.25713
SGTC_615k087-0205167 μM0.3448289642773ChemDiv (Drug-like library)539.964245.8418
SGTC_22436634756200 μM0.344828885676Chembridge (Fragment library)234.079262.01512
SGTC_6260214-000933 μM0.3442623437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress