0469-0687

3-(4-nitrobenzoyl)-1,3-benzoxazol-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1248
Screen concentration 58.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 4017482
SMILES C1=CC=C2C(=C1)N(C(=O)O2)C(=O)C3=CC=C(C=C3)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(cc1)C(=O)N2C(=O)Oc3ccccc23
Molecular weight 284.2237
ALogP 2.89
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.59
% growth inhibition (Hom. pool) 5.13


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4017482
Download HIP data (tab-delimited text)  (excel)
Gene:DCP2(YNL118C)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.01||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:EMG1(YLR186W)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:FRS1(YLR060W)|FD-Score:12.4|P-value:1.48E-35|Clearance:3.59||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:FRS2(YFL022C)|FD-Score:9.55|P-value:6.57E-22|Clearance:3.59||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GLN1(YPR035W)|FD-Score:4.3|P-value:8.40E-6|Clearance:0.11||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPI18(YBR004C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.37||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HSP60(YLR259C)|FD-Score:4.96|P-value:3.51E-7|Clearance:0.28||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:ILV5(YLR355C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.18||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:NAN1(YPL126W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.1||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:OLE1(YGL055W)|FD-Score:3.21|P-value:6.70E-4|Clearance:0.08||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PWP1(YLR196W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.47||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RPL32(YBL092W)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPM2(YML091C)|FD-Score:-3.77|P-value:8.07E-5|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RRN3(YKL125W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:TAH18(YPR048W)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TRE2(YOR256C)|FD-Score:3.13|P-value:8.78E-4|Clearance:0.01||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YAH1(YPL252C)|FD-Score:5.96|P-value:1.24E-9|Clearance:0.18||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YDR396W(YDR396W_d)|FD-Score:-3.89|P-value:4.94E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YFH1(YDL120W)|FD-Score:3.11|P-value:9.34E-4|Clearance:0.1||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YGR115C(YGR115C_d)|FD-Score:3.72|P-value:9.95E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJR141W(YJR141W_p)|FD-Score:5.78|P-value:3.68E-9|Clearance:0.82||SGD DESC:Essential protein of unknown function Gene:DCP2(YNL118C)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.01||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:EMG1(YLR186W)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:FRS1(YLR060W)|FD-Score:12.4|P-value:1.48E-35|Clearance:3.59||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:FRS2(YFL022C)|FD-Score:9.55|P-value:6.57E-22|Clearance:3.59||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GLN1(YPR035W)|FD-Score:4.3|P-value:8.40E-6|Clearance:0.11||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPI18(YBR004C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.37||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HSP60(YLR259C)|FD-Score:4.96|P-value:3.51E-7|Clearance:0.28||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:ILV5(YLR355C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.18||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:NAN1(YPL126W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.1||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:OLE1(YGL055W)|FD-Score:3.21|P-value:6.70E-4|Clearance:0.08||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PWP1(YLR196W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.47||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RPL32(YBL092W)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPM2(YML091C)|FD-Score:-3.77|P-value:8.07E-5|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RRN3(YKL125W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:TAH18(YPR048W)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TRE2(YOR256C)|FD-Score:3.13|P-value:8.78E-4|Clearance:0.01||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YAH1(YPL252C)|FD-Score:5.96|P-value:1.24E-9|Clearance:0.18||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YDR396W(YDR396W_d)|FD-Score:-3.89|P-value:4.94E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YFH1(YDL120W)|FD-Score:3.11|P-value:9.34E-4|Clearance:0.1||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YGR115C(YGR115C_d)|FD-Score:3.72|P-value:9.95E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJR141W(YJR141W_p)|FD-Score:5.78|P-value:3.68E-9|Clearance:0.82||SGD DESC:Essential protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4017482
Download HOP data (tab-delimited text)  (excel)
Gene:AGX1(YFL030W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:ALG8(YOR067C)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ALK2(YBL009W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:APM3(YBR288C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARN1(YHL040C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:ATG7(YHR171W)|FD-Score:4.77|P-value:9.17E-7||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP10(YLR393W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BIO3(YNR058W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BNI1(YNL271C)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BSC4(YNL269W)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BST1(YFL025C)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CHS5(YLR330W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COG6(YNL041C)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPT1(YNL130C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAK1(YML070W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL1(YIR027C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DDR48(YMR173W)|FD-Score:-4.64|P-value:1.78E-6||SGD DESC:DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress Gene:DSE2(YHR143W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUR3(YHL016C)|FD-Score:5.34|P-value:4.58E-8||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:DYN1(YKR054C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ELP4(YPL101W)|FD-Score:4.28|P-value:9.15E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EOS1(YNL080C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERT1(YBR239C_p)|FD-Score:-4.48|P-value:3.80E-6||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:EXO1(YOR033C)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FMO1(YHR176W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FUB1(YCR076C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV10(YIL097W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAL1(YBR020W)|FD-Score:7.99|P-value:6.65E-16||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCV1(YDR019C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GEA1(YJR031C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GRH1(YDR517W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress Gene:GUT2(YIL155C)|FD-Score:4.39|P-value:5.75E-6||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HEM14(YER014W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IDP2(YLR174W)|FD-Score:6.9|P-value:2.63E-12||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IGD1(YFR017C)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IPT1(YDR072C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KIN3(YAR018C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:KTR6(YPL053C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:-4.66|P-value:1.57E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM21(YDR318W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MET3(YJR010W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MRL1(YPR079W)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress Gene:NAT3(YPR131C)|FD-Score:-4.79|P-value:8.19E-7||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NEW1(YPL226W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NTG1(YAL015C)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:NTO1(YPR031W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OGG1(YML060W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OSW2(YLR054C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PDR18(YNR070W_p)|FD-Score:5.25|P-value:7.61E-8||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX29(YDR479C)|FD-Score:-4.64|P-value:1.71E-6||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PGM3(YMR278W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIL1(YGR086C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:POF1(YCL047C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PPT2(YPL148C)|FD-Score:-9.45|P-value:1.66E-21||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PSY2(YNL201C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PXL1(YKR090W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RDI1(YDL135C)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:REC114(YMR133W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:RGC1(YPR115W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS0A(YGR214W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:7.18|P-value:3.39E-13||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SDP1(YIL113W)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SIZ1(YDR409W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SOD2(YHR008C)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOK2(YMR016C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SPG1(YGR236C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SRL2(YLR082C)|FD-Score:8.19|P-value:1.25E-16||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SWI3(YJL176C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TFB6(YOR352W)|FD-Score:-4.44|P-value:4.60E-6||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TPO5(YKL174C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:TUF1(YOR187W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBX6(YJL048C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:UFD4(YKL010C)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URM1(YIL008W)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:YBR063C(YBR063C_p)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YBR139W(YBR139W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YCR050C(YCR050C_p)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YCR051W(YCR051W_p)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR095W-A(YCR095W-A_p)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Putative protein of unknown function Gene:YDR210W(YDR210W_p)|FD-Score:5.38|P-value:3.81E-8||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR250C(YDR250C_d)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YER010C(YER010C)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YET2(YMR040W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIP4(YGL198W)|FD-Score:5.1|P-value:1.68E-7||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-3.98|P-value:3.52E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL218W(YJL218W_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YJR154W(YJR154W_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKE4(YIL023C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL031W(YKL031W_d)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL118W(YKL118W_d)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR040C(YKR040C_d)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YLR297W(YLR297W_p)|FD-Score:-4.8|P-value:7.75E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YLR422W(YLR422W_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YML057C-A(YML057C-A_d)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR265C(YMR265C_p)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Putative protein of unknown function Gene:YMR310C(YMR310C_p)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL037C(YOL037C_d)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOL050C(YOL050C_d)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR223W(YOR223W)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPK3(YBR028C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPR108W-A(YPR108W-A_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZEO1(YOL109W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:AGX1(YFL030W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:ALG8(YOR067C)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ALK2(YBL009W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:APM3(YBR288C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARN1(YHL040C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:ATG7(YHR171W)|FD-Score:4.77|P-value:9.17E-7||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP10(YLR393W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BIO3(YNR058W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BNI1(YNL271C)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BSC4(YNL269W)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BST1(YFL025C)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CHS5(YLR330W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COG6(YNL041C)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPT1(YNL130C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAK1(YML070W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DAL1(YIR027C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DDR48(YMR173W)|FD-Score:-4.64|P-value:1.78E-6||SGD DESC:DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress Gene:DSE2(YHR143W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUR3(YHL016C)|FD-Score:5.34|P-value:4.58E-8||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:DYN1(YKR054C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ELP4(YPL101W)|FD-Score:4.28|P-value:9.15E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EOS1(YNL080C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERT1(YBR239C_p)|FD-Score:-4.48|P-value:3.80E-6||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:EXO1(YOR033C)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FMO1(YHR176W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FUB1(YCR076C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV10(YIL097W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAL1(YBR020W)|FD-Score:7.99|P-value:6.65E-16||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCV1(YDR019C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GEA1(YJR031C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GRH1(YDR517W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress Gene:GUT2(YIL155C)|FD-Score:4.39|P-value:5.75E-6||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HEM14(YER014W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IDP2(YLR174W)|FD-Score:6.9|P-value:2.63E-12||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IGD1(YFR017C)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IPT1(YDR072C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KIN3(YAR018C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:KTR6(YPL053C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:-4.66|P-value:1.57E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM21(YDR318W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MET3(YJR010W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MRL1(YPR079W)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress Gene:NAT3(YPR131C)|FD-Score:-4.79|P-value:8.19E-7||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NEW1(YPL226W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NTG1(YAL015C)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:NTO1(YPR031W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OGG1(YML060W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OSW2(YLR054C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PDR18(YNR070W_p)|FD-Score:5.25|P-value:7.61E-8||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX29(YDR479C)|FD-Score:-4.64|P-value:1.71E-6||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PGM3(YMR278W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIL1(YGR086C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:POF1(YCL047C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PPT2(YPL148C)|FD-Score:-9.45|P-value:1.66E-21||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PSY2(YNL201C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PXL1(YKR090W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RDI1(YDL135C)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:REC114(YMR133W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:RGC1(YPR115W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS0A(YGR214W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:7.18|P-value:3.39E-13||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SDP1(YIL113W)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SIZ1(YDR409W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SOD2(YHR008C)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOK2(YMR016C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SPG1(YGR236C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SRL2(YLR082C)|FD-Score:8.19|P-value:1.25E-16||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SWI3(YJL176C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TFB6(YOR352W)|FD-Score:-4.44|P-value:4.60E-6||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TPO5(YKL174C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:TUF1(YOR187W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBX6(YJL048C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:UFD4(YKL010C)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URM1(YIL008W)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:YBR063C(YBR063C_p)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YBR139W(YBR139W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YCR050C(YCR050C_p)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YCR051W(YCR051W_p)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR095W-A(YCR095W-A_p)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Putative protein of unknown function Gene:YDR210W(YDR210W_p)|FD-Score:5.38|P-value:3.81E-8||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR250C(YDR250C_d)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YER010C(YER010C)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YET2(YMR040W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIP4(YGL198W)|FD-Score:5.1|P-value:1.68E-7||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-3.98|P-value:3.52E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL218W(YJL218W_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YJR154W(YJR154W_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKE4(YIL023C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL031W(YKL031W_d)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL118W(YKL118W_d)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR040C(YKR040C_d)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YLR297W(YLR297W_p)|FD-Score:-4.8|P-value:7.75E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YLR422W(YLR422W_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YML057C-A(YML057C-A_d)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR265C(YMR265C_p)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Putative protein of unknown function Gene:YMR310C(YMR310C_p)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL037C(YOL037C_d)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOL050C(YOL050C_d)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR223W(YOR223W)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPK3(YBR028C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPR108W-A(YPR108W-A_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZEO1(YOL109W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YLR060W12.401.48E-353.59FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YFL022C9.556.57E-223.59FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YPL252C5.961.24E-90.18YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YJR141W_p5.783.68E-90.82YJR141W_pEssential protein of unknown function
YLR259C4.963.51E-70.28HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YBR004C4.681.46E-60.37GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YPR035W4.308.40E-60.11GLN1Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YLR196W4.191.40E-50.47PWP1Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
YGR115C_d3.729.95E-50.14YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YPL126W3.581.73E-40.10NAN1U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
YKL125W3.482.51E-40.00RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YBL092W3.482.53E-40.09RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YLR355C3.393.53E-40.18ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YGL055W3.216.70E-40.08OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YOR256C3.138.78E-40.01TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR082C8.191.25E-16SRL2Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
YBR020W7.996.65E-16GAL1Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
YPL183C7.183.39E-13RTT10WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
YLR174W6.902.63E-12IDP2Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication
YDR210W_p5.383.81E-8YDR210W_pPredicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YHL016C5.344.58E-8DUR3Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
YNR070W_p5.257.61E-8PDR18_pPutative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOR033C5.151.30E-7EXO15'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress
YGL198W5.101.68E-7YIP4Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
YCR050C_p5.012.69E-7YCR050C_pNon-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein
YPR079W4.934.10E-7MRL1Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
YHR171W4.779.17E-7ATG7Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation
YMR016C4.671.47E-6SOK2Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
YKR054C4.592.22E-6DYN1Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p
YIL155C4.395.75E-6GUT2Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner

GO enrichment analysis for SGTC_1248
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1918.88E-50SGTC_608k048-0125 159.0 μMChemDiv (Drug-like library)68176520.0945946
0.1851.13E-46SGTC_712r015-0009 80.6 μMChemDiv (Drug-like library)68138720.0625
0.0594.78E-6SGTC_1799st045822 46.1 μMTimTec (Natural product derivative library)36749920.0941176
0.0594.87E-6SGTC_29147968218 45.3 μMChembridge (Drug-like library)29771110.0649351
0.0588.14E-6SGTC_12460422-0085 105.0 μMChemDiv (Drug-like library)2642950.2
0.0588.93E-6SGTC_30519093778 49.5 μMChembridge (Drug-like library)172173780.125
0.0481.93E-4SGTC_1470k295-0292 231.0 μMChemDiv (Drug-like library)50456120.135135
0.0473.31E-4SGTC_12910993-0025 5.8 μMChemDiv (Drug-like library)43533560.078125
0.0464.02E-4SGTC_14934348-0038 111.0 μMChemDiv (Drug-like library)8004240.0735294RSC complex & mRNA processing
0.0447.30E-4SGTC_22629014885 67.5 μMChembridge (Fragment library)64574240.129032
0.0439.31E-4SGTC_9551216-0110 33.0 μMChemDiv (Drug-like library)36197190.0869565sphingolipid biosynthesis & PDR1
0.0420.00120SGTC_13441488-0097 29.1 μMChemDiv (Drug-like library)7479660.0746269
0.0400.00217SGTC_11961588-0294 184.0 μMChemDiv (Drug-like library)61442690.0694444
0.0400.00239SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.212766RNA pol III & RNase P/MRP
0.0390.00246SGTC_2576guaiol 23.1 μMMicrosource (Natural product library)2278290.016129

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7450469-0705296 μM0.53486736ChemDiv (Drug-like library)238.153741.86706mitochondrial processes
SGTC_1813st0512468.6 μM0.3877555291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_12320279-0259161 μM0.3442621560000ChemDiv (Drug-like library)298.27192.63607
SGTC_8360330-01041.35 μM0.3446494820ChemDiv (Drug-like library)294.518543.6103
SGTC_990109-0120622.8 μM0.333333759414ChemDiv (Drug-like library)321.126163.25713
SGTC_850868-0259160.06 μM0.32692383173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_3710195-00067.36 μM0.3191493928593ChemDiv (Drug-like library)253.209682.70204
SGTC_8470342-02745.54 μM0.312518097ChemDiv (Drug-like library)218.165641.28204
SGTC_9262268-140159.4 μM0.3114752931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_2701st07776714.72 μM0.3090915721188TimTec (Natural product derivative library)281.329044.46903