0485-0349

6-[(2-adamantylamino)methylidene]-4-bromocyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1250
Screen concentration 40.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 6842794
SMILES C1C2CC3CC1CC(C2)C3NC=C4C=C(C=CC4=O)Br
Standardized SMILES Oc1ccc(Br)cc1C=NC2C3CC4CC(CC2C4)C3
Molecular weight 334.2508
ALogP 2.95
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.34
% growth inhibition (Hom. pool) 5.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6842794
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:-3.23|P-value:6.24E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATM1(YMR301C)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.02||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:BMS1(YPL217C)|FD-Score:4.27|P-value:9.87E-6|Clearance:0.12||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:COG4(YPR105C)|FD-Score:5.68|P-value:6.91E-9|Clearance:0.49||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERG11(YHR007C)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.08||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GCD11(YER025W)|FD-Score:3.83|P-value:6.40E-5|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.16||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:-3.64|P-value:1.39E-4|Clearance:0||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPI19(YDR437W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.13||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:GPN2(YOR262W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.04||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HSH49(YOR319W)|FD-Score:5.02|P-value:2.57E-7|Clearance:0.04||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NAF1(YNL124W)|FD-Score:-3.48|P-value:2.49E-4|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NMD3(YHR170W)|FD-Score:-5.9|P-value:1.80E-9|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP56(YLR197W)|FD-Score:5.18|P-value:1.08E-7|Clearance:0.16||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PUP1(YOR157C)|FD-Score:3.26|P-value:5.56E-4|Clearance:0.02||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RHO1(YPR165W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:ROK1(YGL171W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.06||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPC19(YNL113W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.02||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL18A(YOL120C)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN2(YIL075C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RRN3(YKL125W)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.1||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEC13(YLR208W)|FD-Score:3.27|P-value:5.34E-4|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:13.9|P-value:6.00E-44|Clearance:8.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SUI3(YPL237W)|FD-Score:3.79|P-value:7.49E-5|Clearance:0.14||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF11(YMR260C)|FD-Score:4.62|P-value:1.94E-6|Clearance:0.35||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF34(YMR146C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.19||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.02||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP20(YBL004W)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.01||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YGR115C(YGR115C_d)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR076C(YLR076C_d)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPL238C(YPL238C_d)|FD-Score:4.98|P-value:3.17E-7|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ARC40(YBR234C)|FD-Score:-3.23|P-value:6.24E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATM1(YMR301C)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.02||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:BMS1(YPL217C)|FD-Score:4.27|P-value:9.87E-6|Clearance:0.12||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:COG4(YPR105C)|FD-Score:5.68|P-value:6.91E-9|Clearance:0.49||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERG11(YHR007C)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.08||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GCD11(YER025W)|FD-Score:3.83|P-value:6.40E-5|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.16||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:-3.64|P-value:1.39E-4|Clearance:0||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPI19(YDR437W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.13||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:GPN2(YOR262W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.04||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HSH49(YOR319W)|FD-Score:5.02|P-value:2.57E-7|Clearance:0.04||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NAF1(YNL124W)|FD-Score:-3.48|P-value:2.49E-4|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NMD3(YHR170W)|FD-Score:-5.9|P-value:1.80E-9|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP56(YLR197W)|FD-Score:5.18|P-value:1.08E-7|Clearance:0.16||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PUP1(YOR157C)|FD-Score:3.26|P-value:5.56E-4|Clearance:0.02||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RHO1(YPR165W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:ROK1(YGL171W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.06||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPC19(YNL113W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.02||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL18A(YOL120C)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN2(YIL075C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RRN3(YKL125W)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.1||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEC13(YLR208W)|FD-Score:3.27|P-value:5.34E-4|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:13.9|P-value:6.00E-44|Clearance:8.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SUI3(YPL237W)|FD-Score:3.79|P-value:7.49E-5|Clearance:0.14||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF11(YMR260C)|FD-Score:4.62|P-value:1.94E-6|Clearance:0.35||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF34(YMR146C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.19||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.02||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP20(YBL004W)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.01||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YGR115C(YGR115C_d)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR076C(YLR076C_d)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPL238C(YPL238C_d)|FD-Score:4.98|P-value:3.17E-7|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6842794
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT2(YPL202C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AGA2(YGL032C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM34(YMR003W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARE2(YNR019W)|FD-Score:-4.38|P-value:6.06E-6||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARG2(YJL071W)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARO2(YGL148W)|FD-Score:4.33|P-value:7.29E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BNI1(YNL271C)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRE5(YNR051C)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BSC1(YDL037C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BSC5(YNR069C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BST1(YFL025C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD14(YAR014C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:12.5|P-value:5.53E-36||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.02|P-value:2.56E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ENV10(YLR065C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERC1(YHR032W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FAR7(YFR008W)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FCJ1(YKR016W)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FET4(YMR319C)|FD-Score:5.33|P-value:5.05E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE3(YOR381W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE8(YLR047C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GAL1(YBR020W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCN4(YEL009C)|FD-Score:5.34|P-value:4.57E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPD1(YDL022W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GUP1(YGL084C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HPT1(YDR399W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:HXT4(YHR092C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:IMA1(YGR287C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:INO4(YOL108C)|FD-Score:4.36|P-value:6.37E-6||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IPK1(YDR315C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRS4(YKR019C)|FD-Score:5.21|P-value:9.26E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:KES1(YPL145C)|FD-Score:-5.36|P-value:4.19E-8||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LGE1(YPL055C)|FD-Score:-4.83|P-value:6.69E-7||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP5(YOR196C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MAK3(YPR051W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MCK1(YNL307C)|FD-Score:-4.37|P-value:6.25E-6||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM32(YOR147W)|FD-Score:-7.63|P-value:1.22E-14||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MDM36(YPR083W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MNN2(YBR015C)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRF1(YGL143C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPS5(YBR251W)|FD-Score:-6.45|P-value:5.74E-11||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC2(YDR205W)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSH1(YHR120W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSN4(YKL062W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MTD1(YKR080W)|FD-Score:7.62|P-value:1.26E-14||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NHP10(YDL002C)|FD-Score:4.9|P-value:4.76E-7||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NUM1(YDR150W)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCA2(YNL056W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPI8(YKR035C_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPI9(YLR338W_d)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU23(YLR037C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX13(YLR191W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PYK2(YOR347C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RCF2(YNR018W)|FD-Score:-5.31|P-value:5.38E-8||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:REC107(YJR021C)|FD-Score:-4.43|P-value:4.74E-6||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:REV7(YIL139C)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHB1(YCR027C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIM101(YHL027W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPL2B(YIL018W)|FD-Score:-3.74|P-value:9.04E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL40B(YKR094C)|FD-Score:-5.11|P-value:1.60E-7||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT10(YPL183C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAP1(YER047C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SKN7(YHR206W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLC1(YDL052C)|FD-Score:4.36|P-value:6.57E-6||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SNF6(YHL025W)|FD-Score:-4.27|P-value:9.63E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SOD1(YJR104C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO77(YLR341W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:TCB2(YNL087W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TIF2(YJL138C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TMS1(YDR105C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TRS33(YOR115C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSC3(YBR058C-A)|FD-Score:-6.16|P-value:3.54E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX2(YML013W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VAC8(YEL013W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VID27(YNL212W)|FD-Score:7.6|P-value:1.52E-14||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VPS27(YNR006W)|FD-Score:-3.76|P-value:8.44E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YCR015C(YCR015C_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL144C(YDL144C_p)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR250C(YDR250C_d)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.94|P-value:4.04E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YEA6(YEL006W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGR290W(YGR290W_d)|FD-Score:7.13|P-value:5.06E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJL070C(YJL070C_p)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YJR011C(YJR011C_p)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YJR039W(YJR039W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL096C-B(YKL096C-B_p)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL115C(YKL115C_d)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:-5.68|P-value:6.80E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR078W(YKR078W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR030W(YLR030W_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative protein of unknown function Gene:YLR269C(YLR269C_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML079W(YML079W_p)|FD-Score:5.95|P-value:1.35E-9||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-3.73|P-value:9.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR015W(YOR015W_d)|FD-Score:4.31|P-value:8.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR059C(YOR059C_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YPL199C(YPL199C_p)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR027C(YPR027C_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative protein of unknown function Gene:YPR159C-A(YPR159C-A_p)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR172W(YPR172W_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:AFG3(YER017C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT2(YPL202C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AGA2(YGL032C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM34(YMR003W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARE2(YNR019W)|FD-Score:-4.38|P-value:6.06E-6||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARG2(YJL071W)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARO2(YGL148W)|FD-Score:4.33|P-value:7.29E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BNI1(YNL271C)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRE5(YNR051C)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BSC1(YDL037C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BSC5(YNR069C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BST1(YFL025C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD14(YAR014C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:12.5|P-value:5.53E-36||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.02|P-value:2.56E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ENV10(YLR065C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERC1(YHR032W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FAR7(YFR008W)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FCJ1(YKR016W)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FET4(YMR319C)|FD-Score:5.33|P-value:5.05E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE3(YOR381W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE8(YLR047C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GAL1(YBR020W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCN4(YEL009C)|FD-Score:5.34|P-value:4.57E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPD1(YDL022W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GUP1(YGL084C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HPT1(YDR399W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:HXT4(YHR092C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:IMA1(YGR287C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:INO4(YOL108C)|FD-Score:4.36|P-value:6.37E-6||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IPK1(YDR315C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRS4(YKR019C)|FD-Score:5.21|P-value:9.26E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:KES1(YPL145C)|FD-Score:-5.36|P-value:4.19E-8||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LGE1(YPL055C)|FD-Score:-4.83|P-value:6.69E-7||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP5(YOR196C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MAK3(YPR051W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MCK1(YNL307C)|FD-Score:-4.37|P-value:6.25E-6||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM32(YOR147W)|FD-Score:-7.63|P-value:1.22E-14||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MDM36(YPR083W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MNN2(YBR015C)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRF1(YGL143C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPS5(YBR251W)|FD-Score:-6.45|P-value:5.74E-11||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC2(YDR205W)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSH1(YHR120W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSN4(YKL062W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MTD1(YKR080W)|FD-Score:7.62|P-value:1.26E-14||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NHP10(YDL002C)|FD-Score:4.9|P-value:4.76E-7||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NUM1(YDR150W)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCA2(YNL056W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPI8(YKR035C_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPI9(YLR338W_d)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU23(YLR037C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX13(YLR191W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PYK2(YOR347C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RCF2(YNR018W)|FD-Score:-5.31|P-value:5.38E-8||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:REC107(YJR021C)|FD-Score:-4.43|P-value:4.74E-6||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:REV7(YIL139C)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHB1(YCR027C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIM101(YHL027W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPL2B(YIL018W)|FD-Score:-3.74|P-value:9.04E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL40B(YKR094C)|FD-Score:-5.11|P-value:1.60E-7||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT10(YPL183C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAP1(YER047C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SKN7(YHR206W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLC1(YDL052C)|FD-Score:4.36|P-value:6.57E-6||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SNF6(YHL025W)|FD-Score:-4.27|P-value:9.63E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SOD1(YJR104C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO77(YLR341W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:TCB2(YNL087W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TIF2(YJL138C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TMS1(YDR105C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TRS33(YOR115C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSC3(YBR058C-A)|FD-Score:-6.16|P-value:3.54E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX2(YML013W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VAC8(YEL013W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VID27(YNL212W)|FD-Score:7.6|P-value:1.52E-14||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VPS27(YNR006W)|FD-Score:-3.76|P-value:8.44E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YCR015C(YCR015C_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL144C(YDL144C_p)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR250C(YDR250C_d)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.94|P-value:4.04E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YEA6(YEL006W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGR290W(YGR290W_d)|FD-Score:7.13|P-value:5.06E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJL070C(YJL070C_p)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YJR011C(YJR011C_p)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YJR039W(YJR039W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL096C-B(YKL096C-B_p)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YKL115C(YKL115C_d)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:-5.68|P-value:6.80E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR078W(YKR078W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR030W(YLR030W_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative protein of unknown function Gene:YLR269C(YLR269C_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML079W(YML079W_p)|FD-Score:5.95|P-value:1.35E-9||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-3.73|P-value:9.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR015W(YOR015W_d)|FD-Score:4.31|P-value:8.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR059C(YOR059C_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YPL199C(YPL199C_p)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR027C(YPR027C_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative protein of unknown function Gene:YPR159C-A(YPR159C-A_p)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR172W(YPR172W_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W13.906.00E-448.18SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPR105C5.686.91E-90.49COG4Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR197W5.181.08E-70.16NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YOR319W5.022.57E-70.04HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YPL238C_d4.983.17E-70.27YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YKL125W4.721.20E-60.10RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YMR260C4.621.94E-60.35TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YPL217C4.279.87E-60.12BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YMR146C4.151.66E-50.19TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YDR437W3.963.73E-50.13GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
YER025W3.836.40E-50.02GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YMR301C3.816.94E-50.02ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YPL237W3.797.49E-50.14SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YPR165W3.651.31E-45.47E-4RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YGL171W3.651.31E-40.06ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL005C12.505.53E-36COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YKR080W7.621.26E-14MTD1NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
YNL212W7.601.52E-14VID27Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
YGR290W_d7.135.06E-13YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YML079W_p5.951.35E-9YML079W_pNon-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm
YFR008W5.783.70E-9FAR7Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
YEL009C5.344.57E-8GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YMR319C5.335.05E-8FET4Low-affinity Fe(II) transporter of the plasma membrane
YKR019C5.219.26E-8IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YHR032W5.091.82E-7ERC1Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine
YML071C5.022.56E-7COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDL002C4.904.76E-7NHP10Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
YKL115C_d4.651.64E-6YKL115C_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
YGL084C4.651.70E-6GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YOR002W4.631.85E-6ALG6Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease

GO enrichment analysis for SGTC_1250
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2864.77E-111SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.202899
0.2265.54E-69SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.0657895
0.1982.38E-53SGTC_8550428-0020 25.0 μMChemDiv (Drug-like library)68124140.24Golgi
0.1931.49E-50SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.0571429
0.1862.67E-47SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.078125
0.1851.42E-46SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.259259Golgi
0.1755.68E-42SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.114754
0.1716.19E-40SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.171429Golgi
0.1576.63E-34SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0422535
0.1561.45E-33SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0491803Golgi
0.1432.64E-28SGTC_1751st044839 27.2 μMTimTec (Natural product derivative library)52913260.0958904Golgi
0.1412.13E-27SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.225806Golgi
0.1381.23E-26SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0625
0.1381.79E-26SGTC_12670828-0272 9.5 μMChemDiv (Drug-like library)7453340.0625
0.1361.20E-25SGTC_5660958-0068 16.5 μMChemDiv (Drug-like library)68153650.202899RSC & ERG11

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_6420335-0876158 μM0.4807696812350ChemDiv (Drug-like library)424.373345.41712ubiquinone biosynthesis & proteasome
SGTC_12450418-007617.2 μM0.4545456790114ChemDiv (Drug-like library)417.362544.38714ERG2
SGTC_9611252-1571372 μM0.4081636813498ChemDiv (Drug-like library)288.16421.05124
SGTC_3031348-166834.2 μM0.3921575380509ChemDiv (Drug-like library)292.13131.61324
SGTC_1320417-171525.81 μM0.3389836769098ChemDiv (Drug-like library)409.299024.56114RPP1 & pyrimidine depletion
SGTC_3301488-031980.1 μM0.3389836846614ChemDiv (Drug-like library)392.248663.99223RPP1 & pyrimidine depletion
SGTC_7560508-199928.3 μM0.3389836753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_910417-175158.38 μM0.3278696795624ChemDiv (Drug-like library)409.299024.56114Golgi
SGTC_13221348-107012 μM0.3225816758089ChemDiv (Drug-like library)402.308143.54224
SGTC_310508-197457.24 μM0.3166676810525ChemDiv (Drug-like library)414.679724.6213