0493-0875

N-[4-[6-(4-acetamidophenyl)-2-(4-bromophenyl)pyrimidin-4-yl]phenyl]acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1252
Screen concentration 34.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 3960963
SMILES CC(=O)NC1=CC=C(C=C1)C2=CC(=NC(=N2)C3=CC=C(C=C3)Br)C4=CC=C(C=C4)NC(=O)C
Standardized SMILES CC(=O)Nc1ccc(cc1)c2cc(nc(n2)c3ccc(Br)cc3)c4ccc(NC(=O)C)cc4
Molecular weight 501.3745
ALogP 4.55
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.66
% growth inhibition (Hom. pool) 5.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3960963
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.06||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCL1(YPR025C)|FD-Score:-3.82|P-value:6.66E-5|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC20(YGL116W)|FD-Score:4.22|P-value:1.21E-5|Clearance:0.01||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DBP2(YNL112W)|FD-Score:-3.11|P-value:9.43E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GPM1(YKL152C)|FD-Score:-3.53|P-value:2.05E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:KAP95(YLR347C)|FD-Score:-4.2|P-value:1.31E-5|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LAS17(YOR181W)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:NDD1(YOR372C)|FD-Score:-3.31|P-value:4.68E-4|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NMD3(YHR170W)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.13||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.03||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP15(YNL110C)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:QNS1(YHR074W)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RFC1(YOR217W)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.24||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLP24(YLR009W)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.06||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB10(YOR210W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL18A(YOL120C)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS15(YOL040C)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:4.61|P-value:2.02E-6|Clearance:0.31||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC1(YDR164C)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.26||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SMT3(YDR510W)|FD-Score:-3.14|P-value:8.46E-4|Clearance:0||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPP382(YLR424W)|FD-Score:3.75|P-value:8.82E-5|Clearance:0.09||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SRB4(YER022W)|FD-Score:5.39|P-value:3.60E-8|Clearance:0.51||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:SRB6(YBR253W)|FD-Score:6.41|P-value:7.15E-11|Clearance:1.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:TOA2(YKL058W)|FD-Score:3.16|P-value:7.90E-4|Clearance:0.06||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:ULP1(YPL020C)|FD-Score:-4.39|P-value:5.54E-6|Clearance:0||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:YDR187C(YDR187C_d)|FD-Score:3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YPL142C(YPL142C_d)|FD-Score:4.3|P-value:8.61E-6|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:ACT1(YFL039C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.06||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCL1(YPR025C)|FD-Score:-3.82|P-value:6.66E-5|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC20(YGL116W)|FD-Score:4.22|P-value:1.21E-5|Clearance:0.01||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DBP2(YNL112W)|FD-Score:-3.11|P-value:9.43E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GPM1(YKL152C)|FD-Score:-3.53|P-value:2.05E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:KAP95(YLR347C)|FD-Score:-4.2|P-value:1.31E-5|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LAS17(YOR181W)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:NDD1(YOR372C)|FD-Score:-3.31|P-value:4.68E-4|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NMD3(YHR170W)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.13||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.03||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP15(YNL110C)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:QNS1(YHR074W)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RFC1(YOR217W)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.24||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLP24(YLR009W)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.06||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB10(YOR210W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL18A(YOL120C)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS15(YOL040C)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:4.61|P-value:2.02E-6|Clearance:0.31||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC1(YDR164C)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.26||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SMT3(YDR510W)|FD-Score:-3.14|P-value:8.46E-4|Clearance:0||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPP382(YLR424W)|FD-Score:3.75|P-value:8.82E-5|Clearance:0.09||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SRB4(YER022W)|FD-Score:5.39|P-value:3.60E-8|Clearance:0.51||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:SRB6(YBR253W)|FD-Score:6.41|P-value:7.15E-11|Clearance:1.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:TOA2(YKL058W)|FD-Score:3.16|P-value:7.90E-4|Clearance:0.06||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:ULP1(YPL020C)|FD-Score:-4.39|P-value:5.54E-6|Clearance:0||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:YDR187C(YDR187C_d)|FD-Score:3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YPL142C(YPL142C_d)|FD-Score:4.3|P-value:8.61E-6|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3960963
Download HOP data (tab-delimited text)  (excel)
Gene:ACH1(YBL015W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADO1(YJR105W)|FD-Score:5.62|P-value:9.80E-9||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ALD3(YMR169C)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ALG8(YOR067C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ALK2(YBL009W)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ARG2(YJL071W)|FD-Score:-5.75|P-value:4.44E-9||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARO3(YDR035W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASN2(YGR124W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATF1(YOR377W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:BIO5(YNR056C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CHA1(YCL064C)|FD-Score:-4.96|P-value:3.58E-7||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CKA1(YIL035C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CTS2(YDR371W_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DST1(YGL043W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:DUR3(YHL016C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:EFT2(YDR385W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:FAR7(YFR008W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FOB1(YDR110W)|FD-Score:-5.21|P-value:9.36E-8||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FRE4(YNR060W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUN14(YAL008W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GDB1(YPR184W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GIS2(YNL255C)|FD-Score:6.06|P-value:6.79E-10||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLO1(YML004C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GUP1(YGL084C)|FD-Score:4.36|P-value:6.40E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAP4(YKL109W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HMT1(YBR034C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOP1(YIL072W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:ISU2(YOR226C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:LSM6(YDR378C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCH4(YOL119C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM32(YOR147W)|FD-Score:-7.06|P-value:8.25E-13||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MLS1(YNL117W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MNE1(YOR350C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRN1(YPL184C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MUP3(YHL036W)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NCE101(YJL205C)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:ORM1(YGR038W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH7(YHR001W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEA2(YER149C)|FD-Score:11.1|P-value:1.03E-28||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PEX34(YCL056C)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHO85(YPL031C)|FD-Score:-6.62|P-value:1.84E-11||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PMT7(YDR307W_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PPT2(YPL148C)|FD-Score:-4.43|P-value:4.65E-6||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PSY2(YNL201C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PTC4(YBR125C)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RDI1(YDL135C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RPL14B(YHL001W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL23B(YER117W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL39(YJL189W)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPS0B(YLR048W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS14A(YCR031C)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:4.5|P-value:3.45E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:5.91|P-value:1.70E-9||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SCP1(YOR367W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SGE1(YPR198W)|FD-Score:6.47|P-value:5.04E-11||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SOD1(YJR104C)|FD-Score:5.45|P-value:2.50E-8||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPS100(YHR139C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SPT10(YJL127C)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRB5(YGR104C)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SYF2(YGR129W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TOD6(YBL054W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP5(YGL026C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBX4(YMR067C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:YAL042C-A(YAL042C-A_d)|FD-Score:6.05|P-value:7.18E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBL055C(YBL055C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.36|P-value:6.44E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR141C(YBR141C_p)|FD-Score:5.51|P-value:1.78E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR094W(YDR094W_d)|FD-Score:-5.26|P-value:7.29E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR095C(YDR095C_d)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR186C(YDR186C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.96|P-value:3.45E-7||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGL117W(YGL117W_p)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Putative protein of unknown function Gene:YGR283C(YGR283C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YHR138C(YHR138C_p)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YKL071W(YKL071W_p)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR236C(YLR236C_d)|FD-Score:-4.26|P-value:1.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR241W(YLR241W_p)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR264C-A(YLR264C-A_p)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Putative protein of unknown function Gene:YMR310C(YMR310C_p)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YPK3(YBR028C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL062W(YPL062W_d)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL260W(YPL260W_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR012W(YPR012W_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACH1(YBL015W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADO1(YJR105W)|FD-Score:5.62|P-value:9.80E-9||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ALD3(YMR169C)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ALG8(YOR067C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ALK2(YBL009W)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ARG2(YJL071W)|FD-Score:-5.75|P-value:4.44E-9||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARO3(YDR035W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASN2(YGR124W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATF1(YOR377W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:BIO5(YNR056C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CHA1(YCL064C)|FD-Score:-4.96|P-value:3.58E-7||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CKA1(YIL035C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CTS2(YDR371W_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DST1(YGL043W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:DUR3(YHL016C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:EFT2(YDR385W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:FAR7(YFR008W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FOB1(YDR110W)|FD-Score:-5.21|P-value:9.36E-8||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FRE4(YNR060W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUN14(YAL008W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GDB1(YPR184W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GIS2(YNL255C)|FD-Score:6.06|P-value:6.79E-10||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLO1(YML004C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GUP1(YGL084C)|FD-Score:4.36|P-value:6.40E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAP4(YKL109W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HMT1(YBR034C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOP1(YIL072W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:ISU2(YOR226C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:LSM6(YDR378C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCH4(YOL119C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM32(YOR147W)|FD-Score:-7.06|P-value:8.25E-13||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MLS1(YNL117W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MNE1(YOR350C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRN1(YPL184C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MUP3(YHL036W)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NCE101(YJL205C)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:ORM1(YGR038W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH7(YHR001W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEA2(YER149C)|FD-Score:11.1|P-value:1.03E-28||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PEX34(YCL056C)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHO85(YPL031C)|FD-Score:-6.62|P-value:1.84E-11||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PMT7(YDR307W_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PPT2(YPL148C)|FD-Score:-4.43|P-value:4.65E-6||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PSY2(YNL201C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PTC4(YBR125C)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RDI1(YDL135C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RPL14B(YHL001W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL23B(YER117W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL39(YJL189W)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPS0B(YLR048W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS14A(YCR031C)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:4.5|P-value:3.45E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:5.91|P-value:1.70E-9||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SCP1(YOR367W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SGE1(YPR198W)|FD-Score:6.47|P-value:5.04E-11||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SOD1(YJR104C)|FD-Score:5.45|P-value:2.50E-8||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPS100(YHR139C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SPT10(YJL127C)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRB5(YGR104C)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SYF2(YGR129W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TOD6(YBL054W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP5(YGL026C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBX4(YMR067C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:YAL042C-A(YAL042C-A_d)|FD-Score:6.05|P-value:7.18E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBL055C(YBL055C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.36|P-value:6.44E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR141C(YBR141C_p)|FD-Score:5.51|P-value:1.78E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR094W(YDR094W_d)|FD-Score:-5.26|P-value:7.29E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR095C(YDR095C_d)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR186C(YDR186C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.96|P-value:3.45E-7||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGL117W(YGL117W_p)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Putative protein of unknown function Gene:YGR283C(YGR283C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YHR138C(YHR138C_p)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YKL071W(YKL071W_p)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR236C(YLR236C_d)|FD-Score:-4.26|P-value:1.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR241W(YLR241W_p)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR264C-A(YLR264C-A_p)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Putative protein of unknown function Gene:YMR310C(YMR310C_p)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YPK3(YBR028C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL062W(YPL062W_d)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL260W(YPL260W_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR012W(YPR012W_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR253W6.417.15E-111.03SRB6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YER022W5.393.60E-80.51SRB4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
YDR164C4.875.49E-70.26SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
YJR123W4.612.02E-60.31RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YPL142C_d4.308.61E-60.08YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YGL116W4.221.21E-50.01CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YOL040C4.221.24E-50.10RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YHR170W4.121.90E-50.13NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YOR217W3.993.36E-50.23RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YLR424W3.758.82E-50.09SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YGL123W3.661.25E-40.02RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YOR206W3.641.34E-40.03NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YFL039C3.621.48E-40.06ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YOR210W3.551.90E-40.10RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YOL120C3.452.82E-40.14RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER149C11.101.03E-28PEA2Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth
YPR198W6.475.04E-11SGE1Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
YNL255C6.066.79E-10GIS2Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
YAL042C-A_d6.057.18E-10YAL042C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene
YGL244W5.911.70E-9RTF1Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
YJR105W5.629.80E-9ADO1Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
YML004C5.571.24E-8GLO1Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
YBR141C_p5.511.78E-8YBR141C_pPutative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
YCL056C5.462.36E-8PEX34Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
YJR104C5.452.50E-8SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YLR264C-A_p5.101.73E-7YLR264C-A_pPutative protein of unknown function
YGL117W_p4.621.91E-6YGL117W_pPutative protein of unknown function
YPL062W_d4.621.92E-6YPL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
YGL026C4.621.95E-6TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YJL127C4.532.99E-6SPT10Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box

GO enrichment analysis for SGTC_1252
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0841.06E-10SGTC_12340326-0494 15.5 μMChemDiv (Drug-like library)15478130.267857
0.0814.41E-10SGTC_5831488-1091 88.9 μMChemDiv (Drug-like library)43032730.129032
0.0809.47E-10SGTC_32459134821 49.5 μMChembridge (Drug-like library)170149010.151515
0.0741.03E-8SGTC_2851486-1325 46.0 μMChemDiv (Drug-like library)223102300.190476
0.0741.41E-8SGTC_1941486-1323 56.9 μMChemDiv (Drug-like library)51787540.126761
0.0732.42E-8SGTC_1076itraconazole 331.0 nMMiscellaneous37930.0648148
0.0723.18E-8SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.138889translation
0.0715.30E-8SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.109375TRP & mitochondrial translation
0.0663.22E-7SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.126761
0.0655.15E-7SGTC_2549cedrol 20.9 μMMicrosource (Natural product library)67086650.0166667
0.0648.50E-7SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.15625
0.0648.44E-7SGTC_11604092-0570 10.5 μMChemDiv (Drug-like library)49949750.0985916plasma membrane duress
0.0649.96E-7SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0877193
0.0631.38E-6SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.078125
0.0631.44E-6SGTC_11544088-0362 150.0 μMChemDiv (Drug-like library)66183040.16129

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2845900302442.97 μM0.3773582990173Chembridge (Drug-like library)348.191263.13713amide catabolism
SGTC_22077258803200 μM0.3695653666799Chembridge (Fragment library)244.08521.68212
SGTC_29087930301100 μM0.3636362223178Chembridge (Drug-like library)387.431143.00533
SGTC_12131315-0166115 μM0.339286768401ChemDiv (Drug-like library)266.294562.88713
SGTC_3297911750271.43 μM0.3392867453720Chembridge (Drug-like library)355.210341.93624
SGTC_1080509-0257621.13 μM0.333333536624ChemDiv (Drug-like library)269.381323.3611
SGTC_990109-0120622.8 μM0.326531759414ChemDiv (Drug-like library)321.126163.25713
SGTC_6281636-025316 μM0.324456143ChemDiv (Drug-like library)331.230245.07213sphingolipid biosynthesis & PDR1
SGTC_7950685-026578 μM0.313726302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid
SGTC_2230bromochlorosalicylanilide954.5 nM0.31372677254Miscellaneous326.573063.78522