0550-0035

3-(1-phenylethenyl)-1H-benzimidazole-2-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1254
Screen concentration 264.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 15121435
SMILES C=C(C1=CC=CC=C1)N2C3=CC=CC=C3NC2=S
Standardized SMILES Sc1nc2ccccc2n1C(=C)c3ccccc3
Molecular weight 252.3342
ALogP 4.72
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.14
% growth inhibition (Hom. pool) 9.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 15121435
Download HIP data (tab-delimited text)  (excel)
Gene:CEF1(YMR213W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.05||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:DCP2(YNL118C)|FD-Score:4.37|P-value:6.26E-6|Clearance:0.3||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:ERG1(YGR175C)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.08||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:HCA4(YJL033W)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.37||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:IRR1(YIL026C)|FD-Score:3.77|P-value:8.11E-5|Clearance:0.01||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.08||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LCB1(YMR296C)|FD-Score:4.74|P-value:1.07E-6|Clearance:0.37||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MSS4(YDR208W)|FD-Score:3.76|P-value:8.56E-5|Clearance:0.06||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:PRE6(YOL038W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.31||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP38(YGR075C)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPC17(YJL011C)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL17A(YKL180W)|FD-Score:4.06|P-value:2.41E-5|Clearance:0.29||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPT5(YOR117W)|FD-Score:-3.7|P-value:1.10E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SEN34(YAR008W)|FD-Score:5.14|P-value:1.41E-7|Clearance:0.37||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:STN1(YDR082W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.09||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TFB4(YPR056W)|FD-Score:-4.05|P-value:2.55E-5|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC3(YAL001C)|FD-Score:-3.12|P-value:9.18E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UTP14(YML093W)|FD-Score:-3.31|P-value:4.67E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YKL083W(YKL083W_d)|FD-Score:5.2|P-value:9.72E-8|Clearance:0.37||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YML6(YML025C)|FD-Score:-4.78|P-value:8.88E-7|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YOR102W(YOR102W_d)|FD-Score:4.78|P-value:8.72E-7|Clearance:0.37||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:CEF1(YMR213W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.05||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:DCP2(YNL118C)|FD-Score:4.37|P-value:6.26E-6|Clearance:0.3||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:ERG1(YGR175C)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.08||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:HCA4(YJL033W)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.37||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:IRR1(YIL026C)|FD-Score:3.77|P-value:8.11E-5|Clearance:0.01||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.08||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LCB1(YMR296C)|FD-Score:4.74|P-value:1.07E-6|Clearance:0.37||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MSS4(YDR208W)|FD-Score:3.76|P-value:8.56E-5|Clearance:0.06||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:PRE6(YOL038W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.31||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP38(YGR075C)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPC17(YJL011C)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL17A(YKL180W)|FD-Score:4.06|P-value:2.41E-5|Clearance:0.29||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPT5(YOR117W)|FD-Score:-3.7|P-value:1.10E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SEN34(YAR008W)|FD-Score:5.14|P-value:1.41E-7|Clearance:0.37||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:STN1(YDR082W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.09||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TFB4(YPR056W)|FD-Score:-4.05|P-value:2.55E-5|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC3(YAL001C)|FD-Score:-3.12|P-value:9.18E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UTP14(YML093W)|FD-Score:-3.31|P-value:4.67E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YKL083W(YKL083W_d)|FD-Score:5.2|P-value:9.72E-8|Clearance:0.37||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YML6(YML025C)|FD-Score:-4.78|P-value:8.88E-7|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YOR102W(YOR102W_d)|FD-Score:4.78|P-value:8.72E-7|Clearance:0.37||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 15121435
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AEP2(YMR282C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ALT2(YDR111C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:BIT61(YJL058C)|FD-Score:-4.31|P-value:8.31E-6||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:CIN4(YMR138W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COG6(YNL041C)|FD-Score:-3.77|P-value:8.00E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.48|P-value:3.71E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSN9(YDR179C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:DNF2(YDR093W)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ECM10(YEL030W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ECM22(YLR228C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:EDS1(YBR033W_p)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EHD3(YDR036C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:EMC5(YIL027C)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENT5(YDR153C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:FCY21(YER060W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FES1(YBR101C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GDH1(YOR375C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GLO4(YOR040W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:INM2(YDR287W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:INP53(YOR109W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:MEC3(YLR288C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MIC23(YBL107C_p)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MRT4(YKL009W)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSS11(YMR164C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:MSS2(YDL107W)|FD-Score:-4.5|P-value:3.34E-6||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:MTG2(YHR168W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NAM2(YLR382C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NTH2(YBR001C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAH1(YMR165C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PEP7(YDR323C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PRM7(YDL039C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:PRM9(YAR031W)|FD-Score:7.16|P-value:3.90E-13||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:PTC2(YER089C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RGA2(YDR379W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIM101(YHL027W)|FD-Score:6.08|P-value:6.04E-10||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPA14(YDR156W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL16B(YNL069C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RRT7(YLL030C_d)|FD-Score:6.72|P-value:9.26E-12||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RTS1(YOR014W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT107(YHR154W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SKT5(YBL061C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SNF8(YPL002C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOV1(YMR066W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SPF1(YEL031W)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO11(YHL022C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SRF1(YDL133W)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SWH1(YAR042W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWI4(YER111C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYG1(YIL047C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:THI7(YLR237W)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TPP1(YMR156C)|FD-Score:5.49|P-value:1.99E-8||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:UBX5(YDR330W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:VOA1(YGR106C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS1(YKR001C)|FD-Score:-3.72|P-value:9.93E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS28(YPL065W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS75(YNL246W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WWM1(YFL010C)|FD-Score:4.87|P-value:5.62E-7||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YCL012C(YCL012C)|FD-Score:-4.29|P-value:8.93E-6||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL121C(YDL121C_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein Gene:YDL211C(YDL211C_p)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR455C(YDR455C_d)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL165C(YGL165C_d)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGR130C(YGR130C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHL044W(YHL044W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YHR035W(YHR035W_p)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL202W(YKL202W_d)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR041W(YKR041W)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YLR030W(YLR030W_p)|FD-Score:4.76|P-value:9.57E-7||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:-4.39|P-value:5.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YTA6(YPL074W)|FD-Score:5.66|P-value:7.53E-9||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:AAT2(YLR027C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AEP2(YMR282C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ALT2(YDR111C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:BIT61(YJL058C)|FD-Score:-4.31|P-value:8.31E-6||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:CIN4(YMR138W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COG6(YNL041C)|FD-Score:-3.77|P-value:8.00E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.48|P-value:3.71E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSN9(YDR179C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:DNF2(YDR093W)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ECM10(YEL030W)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ECM22(YLR228C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:EDS1(YBR033W_p)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EHD3(YDR036C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:EMC5(YIL027C)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENT5(YDR153C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:FCY21(YER060W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FES1(YBR101C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GDH1(YOR375C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GLO4(YOR040W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:INM2(YDR287W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:INP53(YOR109W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:MEC3(YLR288C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MIC23(YBL107C_p)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MRT4(YKL009W)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSS11(YMR164C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:MSS2(YDL107W)|FD-Score:-4.5|P-value:3.34E-6||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:MTG2(YHR168W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NAM2(YLR382C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NTH2(YBR001C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAH1(YMR165C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PEP7(YDR323C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PRM7(YDL039C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:PRM9(YAR031W)|FD-Score:7.16|P-value:3.90E-13||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:PTC2(YER089C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RGA2(YDR379W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIM101(YHL027W)|FD-Score:6.08|P-value:6.04E-10||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPA14(YDR156W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL16B(YNL069C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RRT7(YLL030C_d)|FD-Score:6.72|P-value:9.26E-12||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RTS1(YOR014W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT107(YHR154W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SKT5(YBL061C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SNF8(YPL002C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOV1(YMR066W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SPF1(YEL031W)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO11(YHL022C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SRF1(YDL133W)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SWH1(YAR042W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWI4(YER111C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYG1(YIL047C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:THI7(YLR237W)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TPP1(YMR156C)|FD-Score:5.49|P-value:1.99E-8||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:UBX5(YDR330W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:VOA1(YGR106C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS1(YKR001C)|FD-Score:-3.72|P-value:9.93E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS28(YPL065W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS75(YNL246W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WWM1(YFL010C)|FD-Score:4.87|P-value:5.62E-7||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YCL012C(YCL012C)|FD-Score:-4.29|P-value:8.93E-6||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL121C(YDL121C_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein Gene:YDL211C(YDL211C_p)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR455C(YDR455C_d)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL165C(YGL165C_d)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGR130C(YGR130C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHL044W(YHL044W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YHR035W(YHR035W_p)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL202W(YKL202W_d)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR041W(YKR041W)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YLR030W(YLR030W_p)|FD-Score:4.76|P-value:9.57E-7||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:-4.39|P-value:5.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YTA6(YPL074W)|FD-Score:5.66|P-value:7.53E-9||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL083W_d5.209.72E-80.37YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YAR008W5.141.41E-70.37SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
YJL033W4.924.24E-70.37HCA4DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
YOR102W_d4.788.72E-70.37YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YMR296C4.741.07E-60.37LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YNL118C4.376.26E-60.30DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
YKL180W4.062.41E-50.29RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YIL026C3.778.11E-50.01IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDR208W3.768.56E-50.06MSS4Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
YDR367W3.691.10E-40.08KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YDR082W3.611.53E-40.09STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YGR175C3.522.17E-40.08ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YJL011C3.442.87E-40.03RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YMR213W3.413.23E-40.05CEF1Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
YOL038W3.363.86E-40.31PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YAR031W7.163.90E-13PRM9Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family
YLL030C_d6.729.26E-12RRT7_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YHL027W6.086.04E-10RIM101Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
YPL074W5.667.53E-9YTA6Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
YMR156C5.491.99E-8TPP1DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase
YFL010C4.875.62E-7WWM1WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1
YLR030W_p4.769.57E-7YLR030W_pPutative protein of unknown function
YPL247C_p4.711.21E-6YPL247C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
YDR323C4.592.22E-6PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YMR282C4.572.47E-6AEP2Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YLR237W4.513.25E-6THI7Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
YGR106C4.299.00E-6VOA1Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
YER060W4.221.23E-5FCY21Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
YHR154W4.211.28E-5RTT107Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
YNL292W4.171.52E-5PUS4Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)

GO enrichment analysis for SGTC_1254
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0872.08E-11SGTC_12560570-0116 285.0 μMChemDiv (Drug-like library)4106380.0535714
0.0699.56E-8SGTC_11911487-1166 10.6 μMChemDiv (Drug-like library)39557710.0655738azole & statin
0.0691.08E-7SGTC_12590671-0132 43.0 μMChemDiv (Drug-like library)X12590.046875
0.0631.42E-6SGTC_6710560-0003 407.0 μMChemDiv (Drug-like library)32386780.0645161
0.0622.23E-6SGTC_23276189656 200.0 μMChembridge (Fragment library)53490880.125
0.0613.13E-6SGTC_11770983-0204 23.6 μMChemDiv (Drug-like library)7668180.0806452
0.0588.27E-6SGTC_3464513-0042 7.2 μMChemDiv (Drug-like library)28273720.114286azole & statin
0.0571.35E-5SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.0754717
0.0561.79E-5SGTC_13903413-2614 145.0 μMChemDiv (Drug-like library)34214320.0175439fatty acid desaturase (OLE1)
0.0552.34E-5SGTC_5761073-0113 22.7 μMChemDiv (Drug-like library)54395630.0921053redox potentiating
0.0525.43E-5SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.0857143NEO1-PIK1
0.0518.06E-5SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.0416667NEO1
0.0518.90E-5SGTC_24855633444 17.8 μMMiscellaneous57242390.09375redox potentiating
0.0518.99E-5SGTC_13651672-3266 55.4 μMChemDiv (Drug-like library)64158730.0759494
0.0501.21E-4SGTC_32309131665 49.5 μMChembridge (Drug-like library)159437200.05

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21055350118200 μM0.3617022133313Chembridge (Fragment library)249.3322.69913
SGTC_2701st07776714.72 μM0.274515721188TimTec (Natural product derivative library)281.329044.46903
SGTC_1038k292-0204154 μM0.2592594454212ChemDiv (Drug-like library)288.38788413
SGTC_112phenothiazine287.22 μM0.2368427108ChemDiv (Drug-like library)199.271563.66812unfolded protein response
SGTC_6001495-041841.6 μM0.2352945377768ChemDiv (Drug-like library)267.345524.36603RPP1 & pyrimidine depletion
SGTC_2989907364971.43 μM0.22580621367202Chembridge (Drug-like library)352.426863.34613
SGTC_14153966-007178.1 μM0.2173912893185ChemDiv (Drug-like library)251.279923.53202DNA intercalators
SGTC_13321480-065317.5 μM0.2115385749438ChemDiv (Drug-like library)219.259662.79503TSC3-RPN4
SGTC_18835-benzylidenerhodanine20 μM0.2083331273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24545-benzylidenerhodanine10.36 μM0.2083331273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_2706st07823982.5 μM0.20754715434492TimTec (Natural product derivative library)242.273161.08712