Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YKL083W_d | 5.20 | 9.72E-8 | 0.37 | YKL083W_d | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 |
YAR008W | 5.14 | 1.41E-7 | 0.37 | SEN34 | Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease |
YJL033W | 4.92 | 4.24E-7 | 0.37 | HCA4 | DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis |
YOR102W_d | 4.78 | 8.72E-7 | 0.37 | YOR102W_d | Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex |
YMR296C | 4.74 | 1.07E-6 | 0.37 | LCB1 | Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine |
YNL118C | 4.37 | 6.26E-6 | 0.30 | DCP2 | Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress |
YKL180W | 4.06 | 2.41E-5 | 0.29 | RPL17A | Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
YIL026C | 3.77 | 8.11E-5 | 0.01 | IRR1 | Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability |
YDR208W | 3.76 | 8.56E-5 | 0.06 | MSS4 | Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation |
YDR367W | 3.69 | 1.10E-4 | 0.08 | KEI1 | Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene |
YDR082W | 3.61 | 1.53E-4 | 0.09 | STN1 | Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping |
YGR175C | 3.52 | 2.17E-4 | 0.08 | ERG1 | Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine |
YJL011C | 3.44 | 2.87E-4 | 0.03 | RPC17 | RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress |
YMR213W | 3.41 | 3.23E-4 | 0.05 | CEF1 | Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p |
YOL038W | 3.36 | 3.86E-4 | 0.31 | PRE6 | Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress |