0831-1170

6-[(2-benzoyl-4-bromoanilino)methylidene]cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1270
Screen concentration 17.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 6764716
SMILES C1=CC=C(C=C1)C(=O)C2=C(C=CC(=C2)Br)NC=C3C=CC=CC3=O
Standardized SMILES Oc1ccccc1C=Nc2ccc(Br)cc2C(=O)c3ccccc3
Molecular weight 380.2347
ALogP 4.25
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.7
% growth inhibition (Hom. pool) 6.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6764716
Download HIP data (tab-delimited text)  (excel)
Gene:CCT6(YDR188W)|FD-Score:-4.21|P-value:1.25E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CKS1(YBR135W)|FD-Score:4.49|P-value:3.59E-6|Clearance:1.21||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:KRS1(YDR037W)|FD-Score:6.5|P-value:3.92E-11|Clearance:2.02||SGD DESC:Lysyl-tRNA synthetase Gene:PCF11(YDR228C)|FD-Score:3.17|P-value:7.51E-4|Clearance:0.11||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PRE3(YJL001W)|FD-Score:-3.39|P-value:3.56E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPT4(YOR259C)|FD-Score:-3.52|P-value:2.16E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC58(YLR033W)|FD-Score:-3.98|P-value:3.51E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SPT5(YML010W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.1||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TTI1(YKL033W)|FD-Score:-4.55|P-value:2.70E-6|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP7(YER082C)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:CCT6(YDR188W)|FD-Score:-4.21|P-value:1.25E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CKS1(YBR135W)|FD-Score:4.49|P-value:3.59E-6|Clearance:1.21||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:KRS1(YDR037W)|FD-Score:6.5|P-value:3.92E-11|Clearance:2.02||SGD DESC:Lysyl-tRNA synthetase Gene:PCF11(YDR228C)|FD-Score:3.17|P-value:7.51E-4|Clearance:0.11||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PRE3(YJL001W)|FD-Score:-3.39|P-value:3.56E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPT4(YOR259C)|FD-Score:-3.52|P-value:2.16E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC58(YLR033W)|FD-Score:-3.98|P-value:3.51E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SPT5(YML010W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.1||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TTI1(YKL033W)|FD-Score:-4.55|P-value:2.70E-6|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP7(YER082C)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6764716
Download HOP data (tab-delimited text)  (excel)
Gene:ADD37(YMR184W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AGA1(YNR044W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds Gene:AIM39(YOL053W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIR1(YIL079C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COX10(YPL172C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CPT1(YNL130C)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN2(YDR424C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EAF3(YPR023C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:FTH1(YBR207W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:GCN4(YEL009C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLT1(YDL171C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GPA2(YER020W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GYP8(YFL027C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:IPT1(YDR072C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:MET13(YGL125W)|FD-Score:-5.13|P-value:1.48E-7||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MIH1(YMR036C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MOG1(YJR074W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL11(YDL202W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NSR1(YGR159C)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OSM1(YJR051W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PEX5(YDR244W)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PML1(YLR016C)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PPN1(YDR452W)|FD-Score:-3.8|P-value:7.36E-5||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PSP2(YML017W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:RAX2(YLR084C)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RBS1(YDL189W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:REC114(YMR133W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:RGL1(YPL066W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIM4(YHL024W)|FD-Score:-3.84|P-value:6.25E-5||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL24B(YGR148C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRI1(YDL216C)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SAF1(YBR280C)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SPO73(YER046W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SUB1(YMR039C)|FD-Score:5.22|P-value:8.98E-8||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TPA1(YER049W)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:YBR206W(YBR206W_d)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YFL040W(YFL040W_p)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR149W(YGR149W_p)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YIL089W(YIL089W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YLR042C(YLR042C_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YML119W(YML119W_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR111C(YMR111C_p)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR210W(YMR210W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YNL011C(YNL011C_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YNL042W-B(YNL042W-B_p)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Putative protein of unknown function Gene:YOL160W(YOL160W_d)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR300W(YOR300W_d)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YPL067C(YPL067C_p)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL071C(YPL071C_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YRA2(YKL214C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:ZRT3(YKL175W)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency Gene:ADD37(YMR184W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:AGA1(YNR044W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds Gene:AIM39(YOL053W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIR1(YIL079C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COX10(YPL172C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CPT1(YNL130C)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN2(YDR424C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EAF3(YPR023C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:FTH1(YBR207W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:GCN4(YEL009C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLT1(YDL171C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GPA2(YER020W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GYP8(YFL027C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:IPT1(YDR072C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:MET13(YGL125W)|FD-Score:-5.13|P-value:1.48E-7||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MIH1(YMR036C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MOG1(YJR074W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL11(YDL202W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NSR1(YGR159C)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OSM1(YJR051W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PEX5(YDR244W)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PML1(YLR016C)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PPN1(YDR452W)|FD-Score:-3.8|P-value:7.36E-5||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PSP2(YML017W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:RAX2(YLR084C)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RBS1(YDL189W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:REC114(YMR133W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:RGL1(YPL066W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIM4(YHL024W)|FD-Score:-3.84|P-value:6.25E-5||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL24B(YGR148C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRI1(YDL216C)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SAF1(YBR280C)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SPO73(YER046W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SUB1(YMR039C)|FD-Score:5.22|P-value:8.98E-8||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TPA1(YER049W)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:YBR206W(YBR206W_d)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YFL040W(YFL040W_p)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR149W(YGR149W_p)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YIL089W(YIL089W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YLR042C(YLR042C_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YML119W(YML119W_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR111C(YMR111C_p)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR210W(YMR210W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YNL011C(YNL011C_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YNL042W-B(YNL042W-B_p)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Putative protein of unknown function Gene:YOL160W(YOL160W_d)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR300W(YOR300W_d)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YPL067C(YPL067C_p)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL071C(YPL071C_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YRA2(YKL214C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:ZRT3(YKL175W)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR037W6.503.92E-112.02KRS1Lysyl-tRNA synthetase
YBR135W4.493.59E-61.21CKS1Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters
YML010W3.275.31E-40.10SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YDR228C3.177.51E-40.11PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YNL189W3.060.001100.12SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YML023C2.940.001620.09NSE5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YBR167C2.860.002140.04POP7Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YEL058W2.820.002410.08PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YGL048C2.740.003070.08RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YGL150C2.660.003870.07INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YNR038W2.590.004780.02DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YKL022C2.570.005100.02CDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
YOR020C2.550.005360.05HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YKL060C2.510.006110.02FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
YJL085W2.480.006550.01EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR039C5.228.98E-8SUB1Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress
YKL175W5.151.33E-7ZRT3Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency
YNL130C4.963.58E-7CPT1Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication
YLR016C4.602.08E-6PML1Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
YPR023C4.473.99E-6EAF3Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
YER049W4.424.97E-6TPA1Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate
YDL189W4.261.01E-5RBS1Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
YPL066W4.221.20E-5RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YBR207W4.201.31E-5FTH1Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
YPL172C4.112.01E-5COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YPL067C_p3.865.65E-5YPL067C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene
YBR206W_d3.826.70E-5YBR206W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3
YNR044W3.787.72E-5AGA1Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds
YMR111C_p3.778.17E-5YMR111C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
YER020W3.651.31E-4GPA2Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery

GO enrichment analysis for SGTC_1270
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1041.38E-15SGTC_33549151612 71.4 μMChembridge (Drug-like library)272619490.0759494
0.0985.15E-14SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.102941
0.0921.42E-12SGTC_2693st077581 40.7 μMTimTec (Natural product derivative library)252387610.0792079
0.0912.53E-12SGTC_33369143619 71.4 μMChembridge (Drug-like library)455957300.0793651
0.0857.11E-11SGTC_24605552655 174.2 μMMiscellaneous54019890.169231amide catabolism
0.0848.15E-11SGTC_2550chrysin dimethyl ether 71.9 μMMicrosource (Natural product library)888810.115942
0.0822.24E-10SGTC_486niguldipine 82.0 μMMiscellaneous12360.104167amide catabolism
0.0822.71E-10SGTC_33429147791 14.5 μMChembridge (Drug-like library)18381680.0595238
0.0823.13E-10SGTC_1924st058459 75.1 μMTimTec (Natural product derivative library)6886830.0704225
0.0791.30E-9SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.0869565amide catabolism
0.0772.52E-9SGTC_12840929-0043 16.0 μMChemDiv (Drug-like library)12398830.0793651
0.0741.31E-8SGTC_24355706476 10.6 μMMiscellaneous57253750.15942
0.0722.98E-8SGTC_2717st081029 62.3 μMTimTec (Natural product derivative library)52928460.131579
0.0715.07E-8SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.114286amide catabolism
0.0715.27E-8SGTC_21075363541 170.2 μMChembridge (Fragment library)2440520.111111

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_310508-197457.24 μM0.7708336810525ChemDiv (Drug-like library)414.679724.6213
SGTC_7560508-199928.3 μM0.7346946753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_608k048-0125159 μM0.56817652ChemDiv (Drug-like library)350.164082.16525
SGTC_130199-008765 μM0.4901964589825ChemDiv (Drug-like library)354.229326.15902ERG2
SGTC_350133-005464.07 μM0.47826199677ChemDiv (Drug-like library)276.12863.23512
SGTC_80354-000170 μM0.4716984993152ChemDiv (Drug-like library)354.229326.15902
SGTC_1642st00958277.7 μM0.450986739311TimTec (Natural product derivative library)257.241441.48735RPP1 & pyrimidine depletion
SGTC_30833-058795.58 μM0.448985382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_13531498-12068.11 μM0.4117656756808ChemDiv (Drug-like library)371.024062.76223mitochondrial response to ROS
SGTC_8700573-221357.6 μM0.3968253916321ChemDiv (Drug-like library)453.91636.78104ERG2