0849-0096

1-methyl-3-[(E)-2-nitroprop-1-enyl]indole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1273
Screen concentration 14.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 5286025
SMILES CC(=CC1=CN(C2=CC=CC=C21)C)[N+](=O)[O-]
Standardized SMILES CC(=Cc1cn(C)c2ccccc12)[N+](=O)[O-]
Molecular weight 216.2359
ALogP 2.82
H-bond donor count 0
H-bond acceptor count 2
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.63
% growth inhibition (Hom. pool) 7.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5286025
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:11.6|P-value:1.31E-31|Clearance:5.39||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAK1(YFL029C)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.13||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT4(YDL143W)|FD-Score:3.78|P-value:7.73E-5|Clearance:0.23||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DAD2(YKR083C)|FD-Score:6.25|P-value:2.04E-10|Clearance:0.03||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:GCD2(YGR083C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.02||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:KRE29(YER038C)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.11||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LSM5(YER146W)|FD-Score:3.16|P-value:7.88E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NRD1(YNL251C)|FD-Score:3.9|P-value:4.85E-5|Clearance:0.11||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:REB1(YBR049C)|FD-Score:4|P-value:3.15E-5|Clearance:0.1||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.22|P-value:2.48E-10|Clearance:1.69||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RTS2(YOR077W)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.08||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:TFC4(YGR047C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.08||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TRS31(YDR472W)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TTI1(YKL033W)|FD-Score:4.53|P-value:3.01E-6|Clearance:0.38||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YDL152W(YDL152W_d)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:ASA1(YPR085C)|FD-Score:11.6|P-value:1.31E-31|Clearance:5.39||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAK1(YFL029C)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.13||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT4(YDL143W)|FD-Score:3.78|P-value:7.73E-5|Clearance:0.23||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DAD2(YKR083C)|FD-Score:6.25|P-value:2.04E-10|Clearance:0.03||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:GCD2(YGR083C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.02||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:KRE29(YER038C)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.11||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LSM5(YER146W)|FD-Score:3.16|P-value:7.88E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NRD1(YNL251C)|FD-Score:3.9|P-value:4.85E-5|Clearance:0.11||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:REB1(YBR049C)|FD-Score:4|P-value:3.15E-5|Clearance:0.1||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.22|P-value:2.48E-10|Clearance:1.69||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RTS2(YOR077W)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.08||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:TFC4(YGR047C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.08||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TRS31(YDR472W)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TTI1(YKL033W)|FD-Score:4.53|P-value:3.01E-6|Clearance:0.38||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YDL152W(YDL152W_d)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5286025
Download HOP data (tab-delimited text)  (excel)
Gene:ADP1(YCR011C)|FD-Score:4.96|P-value:3.52E-7||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AIM36(YMR157C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALF1(YNL148C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APN1(YKL114C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:BDF1(YLR399C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:COQ6(YGR255C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:CPR7(YJR032W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTK3(YML112W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DAL82(YNL314W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:EMC2(YJR088C)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:GAL2(YLR081W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GDS1(YOR355W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIP4(YAL031C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:GLE2(YER107C)|FD-Score:6.97|P-value:1.55E-12||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRR1(YJR090C)|FD-Score:-4.97|P-value:3.31E-7||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GYL1(YMR192W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HMG1(YML075C)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOR7(YMR251W-A)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:IKI1(YHR187W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:ILM1(YJR118C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:KRE1(YNL322C)|FD-Score:6.53|P-value:3.33E-11||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KRE28(YDR532C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:MEP3(YPR138C)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MKK2(YPL140C)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:3.75|P-value:8.77E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MOT3(YMR070W)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPD2(YOL088C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPL8(YJL063C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NOT5(YPR072W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAC1(YKL120W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OTU2(YHL013C)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PTC7(YHR076W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RNY1(YPL123C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPA12(YJR063W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPN4(YDL020C)|FD-Score:4.34|P-value:7.05E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAM1(YLR180W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SGM1(YJR134C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SKI3(YPR189W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLT2(YHR030C)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF6(YHL025W)|FD-Score:-8.64|P-value:2.84E-18||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNF7(YLR025W)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOL3(YHR163W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:7.45|P-value:4.81E-14||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA16(YHR026W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:XBP1(YIL101C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL083C(YBL083C_d)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBL094C(YBL094C_d)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR259W(YBR259W_p)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCR101C(YCR101C_p)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR290W(YDR290W_d)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER137C(YER137C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative protein of unknown function Gene:YGL072C(YGL072C_d)|FD-Score:5.36|P-value:4.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR250C(YGR250C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YGR291C(YGR291C_d)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJR020W(YJR020W_d)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLL007C(YLL007C_p)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR125W(YLR125W_p)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR171W(YLR171W_d)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR358C(YLR358C_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YOL163W(YOL163W_p)|FD-Score:5.22|P-value:9.15E-8||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:ADP1(YCR011C)|FD-Score:4.96|P-value:3.52E-7||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AIM36(YMR157C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALF1(YNL148C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APN1(YKL114C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:BDF1(YLR399C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:COQ6(YGR255C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:CPR7(YJR032W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTK3(YML112W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DAL82(YNL314W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:EMC2(YJR088C)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:GAL2(YLR081W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GDS1(YOR355W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIP4(YAL031C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:GLE2(YER107C)|FD-Score:6.97|P-value:1.55E-12||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRR1(YJR090C)|FD-Score:-4.97|P-value:3.31E-7||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GYL1(YMR192W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HMG1(YML075C)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOR7(YMR251W-A)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:IKI1(YHR187W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:ILM1(YJR118C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:KRE1(YNL322C)|FD-Score:6.53|P-value:3.33E-11||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KRE28(YDR532C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:MEP3(YPR138C)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MKK2(YPL140C)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:3.75|P-value:8.77E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MOT3(YMR070W)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPD2(YOL088C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPL8(YJL063C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NOT5(YPR072W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OAC1(YKL120W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OTU2(YHL013C)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PTC7(YHR076W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RNY1(YPL123C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:RPA12(YJR063W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPN4(YDL020C)|FD-Score:4.34|P-value:7.05E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAM1(YLR180W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SGM1(YJR134C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SKI3(YPR189W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLT2(YHR030C)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF6(YHL025W)|FD-Score:-8.64|P-value:2.84E-18||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNF7(YLR025W)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOL3(YHR163W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:7.45|P-value:4.81E-14||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA16(YHR026W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:XBP1(YIL101C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL083C(YBL083C_d)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBL094C(YBL094C_d)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR259W(YBR259W_p)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCR101C(YCR101C_p)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR290W(YDR290W_d)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER137C(YER137C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative protein of unknown function Gene:YGL072C(YGL072C_d)|FD-Score:5.36|P-value:4.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR250C(YGR250C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YGR291C(YGR291C_d)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJR020W(YJR020W_d)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLL007C(YLL007C_p)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR125W(YLR125W_p)|FD-Score:4.31|P-value:8.18E-6||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR171W(YLR171W_d)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR358C(YLR358C_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YOL163W(YOL163W_p)|FD-Score:5.22|P-value:9.15E-8||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR085C11.601.31E-315.39ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YKR083C6.252.04E-100.03DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YHR062C6.222.48E-101.69RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL033W4.533.01E-60.38TTI1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
YGR083C4.151.67E-50.02GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YFL029C4.131.80E-50.13CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YBR049C4.003.15E-50.10REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YNL251C3.904.85E-50.11NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YDL143W3.787.73E-50.23CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR047C3.551.93E-40.08TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YER038C3.472.65E-40.11KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YDL152W_d3.363.91E-40.20YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YER146W3.167.88E-40.01LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YDR472W3.158.12E-40.06TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YOR077W3.099.91E-40.08RTS2Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A7.454.81E-14TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YER107C6.971.55E-12GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YNL322C6.533.33E-11KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YGL072C_d5.364.26E-8YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YOL163W_p5.229.15E-8YOL163W_pPutative protein of unknown function; member of the Dal5p subfamily of the major facilitator family
YCR011C4.963.52E-7ADP1Putative ATP-dependent permease of the ABC transporter family of proteins
YJL016W_p4.885.42E-7YJL016W_pPutative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species
YPR138C4.366.39E-6MEP3Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease
YGR255C4.356.86E-6COQ6Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
YDL020C4.347.05E-6RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR125W_p4.318.18E-6YLR125W_pPutative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
YER137C_p4.261.03E-5YER137C_pPutative protein of unknown function
YNL148C4.191.40E-5ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YLR025W4.062.42E-5SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YLR399C4.062.46E-5BDF1Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p

GO enrichment analysis for SGTC_1273
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2436.32E-80SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.140625
0.2411.87E-78SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.122807
0.2312.66E-72SGTC_25732',4'-dihydroxychalcone 15.0 μMTimTec (Pure natural product library)53572180.145455
0.2182.28E-64SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.031746TSC3-RPN4
0.2161.64E-63SGTC_25742',4'-dihydroxychalcone 14.6 μMTimTec (Pure natural product library)53572180.145455
0.2131.81E-61SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.0731707TSC3-RPN4
0.2112.30E-60SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.108108TSC3-RPN4
0.2104.46E-60SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.131148
0.2086.53E-59SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.145455
0.2072.06E-58SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.175439TSC3-RPN4
0.2062.27E-57SGTC_6390833-0042 114.0 μMChemDiv (Drug-like library)59789110.129032
0.2021.49E-55SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.166667TSC3-RPN4
0.2004.63E-54SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.145455
0.1964.49E-52SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0897436TSC3-RPN4
0.1944.83E-51SGTC_720r052-1644 49.9 μMChemDiv (Drug-like library)14228630.121212TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8350312-001067.2 μM0.3673471626196ChemDiv (Drug-like library)213.231942.23302
SGTC_1845st0567533.01 μM0.345375839TimTec (Natural product derivative library)207.182762.09104TSC3-RPN4
SGTC_22567954252200 μM0.322972920Chembridge (Fragment library)213.27832.26802mitochondrial processes
SGTC_10503232-115669.3 μM0.288462700529ChemDiv (Drug-like library)237.276523.0311RSC complex & mRNA processing
SGTC_12941000-1494117 μM0.285714712797ChemDiv (Drug-like library)281.266143.25604
SGTC_210849-011322.99 μM0.281560156ChemDiv (Drug-like library)188.182722.10312
SGTC_467ro 31-822072.8 μM0.2727275083ICCB bioactive library457.547423.86634calcium & mitochondrial duress
SGTC_8860866-0150153 μM0.254902271463ChemDiv (Drug-like library)199.248423.14201
SGTC_120849-00999.27 μM0.256071388ChemDiv (Drug-like library)246.21881.95814
SGTC_490717-096532.13 μM0.2459025889349ChemDiv (Drug-like library)279.293324.0791360S ribosome export