0867-0008

8-methoxy-4-phenyl-1,3-dihydro-1,5-benzodiazepine-2-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1275
Screen concentration 21.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 3422836
SMILES COC1=CC2=C(C=C1)N=C(CC(=S)N2)C3=CC=CC=C3
Standardized SMILES COc1ccc2N=C(CC(=Nc2c1)S)c3ccccc3
Molecular weight 282.3602
ALogP 3.9
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.3
% growth inhibition (Hom. pool) 14.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3422836
Download HIP data (tab-delimited text)  (excel)
Gene:IPP1(YBR011C)|FD-Score:-3.79|P-value:7.42E-5|Clearance:0||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:KRE29(YER038C)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.11||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:NRD1(YNL251C)|FD-Score:4.01|P-value:3.06E-5|Clearance:0.3||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PRI1(YIR008C)|FD-Score:3.17|P-value:7.54E-4|Clearance:0.16||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RLP24(YLR009W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.17||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL10(YLR075W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL3(YOR063W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RRP42(YDL111C)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.3||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP46(YGR095C)|FD-Score:3.75|P-value:8.72E-5|Clearance:0.3||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:WBP1(YEL002C)|FD-Score:4.46|P-value:4.06E-6|Clearance:0.3||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:IPP1(YBR011C)|FD-Score:-3.79|P-value:7.42E-5|Clearance:0||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:KRE29(YER038C)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.11||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:NRD1(YNL251C)|FD-Score:4.01|P-value:3.06E-5|Clearance:0.3||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PRI1(YIR008C)|FD-Score:3.17|P-value:7.54E-4|Clearance:0.16||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RLP24(YLR009W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.17||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL10(YLR075W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL3(YOR063W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RRP42(YDL111C)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.3||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP46(YGR095C)|FD-Score:3.75|P-value:8.72E-5|Clearance:0.3||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:WBP1(YEL002C)|FD-Score:4.46|P-value:4.06E-6|Clearance:0.3||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3422836
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:5.77|P-value:4.06E-9||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AGE2(YIL044C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM29(YKR074W)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:APL6(YGR261C)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARC18(YLR370C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATF1(YOR377W)|FD-Score:4.77|P-value:9.39E-7||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:CTS1(YLR286C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DAL1(YIR027C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIN7(YDR263C)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:FYV5(YCL058C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAL1(YBR020W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:IAH1(YOR126C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRS4(YKR019C)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MLH1(YMR167W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MSY1(YPL097W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:OPI8(YKR035C_d)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PAH1(YMR165C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PEP3(YLR148W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PKH3(YDR466W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRK1(YIL095W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:4|P-value:3.10E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSA1(YPL193W)|FD-Score:4.93|P-value:4.16E-7||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SNX4(YJL036W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SRN2(YLR119W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:STD1(YOR047C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TLG2(YOL018C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA7(YLR262C-A)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:VAC8(YEL013W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS24(YKL041W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:XRS2(YDR369C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YCK1(YHR135C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCR099C(YCR099C_p)|FD-Score:4.36|P-value:6.36E-6||SGD DESC:Putative protein of unknown function Gene:YDL199C(YDL199C_p)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Putative transporter, member of the sugar porter family Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL108C(YGL108C_p)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YHR080C(YHR080C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIM2(YMR151W_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YKE2(YLR200W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR111W(YLR111W_d)|FD-Score:4.77|P-value:9.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function Gene:YLR358C(YLR358C_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YLR374C(YLR374C_d)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMR090W(YMR090W_p)|FD-Score:6.69|P-value:1.08E-11||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function Gene:ZWF1(YNL241C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:ADE4(YMR300C)|FD-Score:5.77|P-value:4.06E-9||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AGE2(YIL044C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM29(YKR074W)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:APL6(YGR261C)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARC18(YLR370C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATF1(YOR377W)|FD-Score:4.77|P-value:9.39E-7||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:CTS1(YLR286C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DAL1(YIR027C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIN7(YDR263C)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:FYV5(YCL058C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAL1(YBR020W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:IAH1(YOR126C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRS4(YKR019C)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MLH1(YMR167W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MSY1(YPL097W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:OPI8(YKR035C_d)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PAH1(YMR165C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PEP3(YLR148W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PKH3(YDR466W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRK1(YIL095W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:4|P-value:3.10E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSA1(YPL193W)|FD-Score:4.93|P-value:4.16E-7||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SNX4(YJL036W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SRN2(YLR119W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:STD1(YOR047C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TLG2(YOL018C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA7(YLR262C-A)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:VAC8(YEL013W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS24(YKL041W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:XRS2(YDR369C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YCK1(YHR135C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCR099C(YCR099C_p)|FD-Score:4.36|P-value:6.36E-6||SGD DESC:Putative protein of unknown function Gene:YDL199C(YDL199C_p)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Putative transporter, member of the sugar porter family Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL108C(YGL108C_p)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YHR080C(YHR080C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIM2(YMR151W_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YKE2(YLR200W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR111W(YLR111W_d)|FD-Score:4.77|P-value:9.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function Gene:YLR358C(YLR358C_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YLR374C(YLR374C_d)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMR090W(YMR090W_p)|FD-Score:6.69|P-value:1.08E-11||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function Gene:ZWF1(YNL241C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL111C4.562.53E-60.30RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YEL002C4.464.06E-60.30WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YLR075W4.231.15E-50.30RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YOR063W4.171.54E-50.30RPL3Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus
YNL251C4.013.06E-50.30NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YGR095C3.758.72E-50.30RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YLR009W3.452.77E-40.17RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YER038C3.285.10E-40.11KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YIR008C3.177.54E-40.16PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YHR190W3.010.001300.02ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YDL164C2.990.001400.12CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YER013W2.870.002080.03PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YJR076C2.830.002310.02CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YML092C2.810.002470.00PRE8Alpha 2 subunit of the 20S proteasome
YLL036C2.810.002500.18PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR090W_p6.691.08E-11YMR090W_pPutative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
YMR300C5.774.06E-9ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YLR262C-A5.042.37E-7TMA7Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
YPL193W4.934.16E-7RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YLR111W_d4.779.34E-7YLR111W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR377W4.779.39E-7ATF1Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
YDR466W4.661.57E-6PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
YPL091W4.444.56E-6GLR1Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
YCR099C_p4.366.36E-6YCR099C_pPutative protein of unknown function
YJL101C4.221.22E-5GSH1Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
YJL036W4.151.65E-5SNX4Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p
YNL241C4.062.44E-5ZWF1Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress
YER056C-A4.003.10E-5RPL34ARibosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication
YLR370C4.003.11E-5ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YGL108C_p3.963.71E-5YGL108C_pProtein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1275
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1095.38E-17SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.0892857exosome
0.1088.44E-17SGTC_2580diallyl disulphide 81.4 μMMicrosource (Natural product library)16590NA
0.1024.24E-15SGTC_2235salicylic acid 500.0 μMMiscellaneous3380.0943396
0.0872.10E-11SGTC_29589077254 16.7 μMChembridge (Drug-like library)164573510.150685
0.0706.44E-8SGTC_1791273-0063 6.8 μMChemDiv (Drug-like library)31081390.0273973
0.0663.37E-7SGTC_28027925815 54.5 μMChembridge (Drug-like library)12629230.075
0.0655.06E-7SGTC_31259127534 49.5 μMChembridge (Drug-like library)16392350.119403
0.0655.55E-7SGTC_1920st075728 45.7 μMTimTec (Natural product derivative library)34003060.0588235
0.0649.33E-7SGTC_10775-fluorocytosine 377.0 nMNIH Clinical Collection33660.0892857exosome
0.0631.35E-6SGTC_24215690543 90.7 μMChembridge (Fragment library)33979930.04
0.0631.35E-6SGTC_31629099653 49.5 μMChembridge (Drug-like library)252375110.102941
0.0612.36E-6SGTC_4235-fluorouracil 457.0 μMMiscellaneous33850.0740741exosome
0.0603.77E-6SGTC_23589037157 4.0 μMChembridge (Fragment library)23889250.216667
0.0595.98E-6SGTC_20194016004 108.0 μMChembridge (Fragment library)455953360.0655738
0.0588.86E-6SGTC_8010866-0028 10.1 μMChemDiv (Drug-like library)68497710.0833333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13833049-003220.7 μM0.293103712864ChemDiv (Drug-like library)291.347084.0261460S ribosome export
SGTC_18693',7'-dimethoxyflavone40.3 μM0.293103688664TimTec (Natural product derivative library)282.290662.80804
SGTC_25623',7'-dimethoxyflavone64.92 μM0.293103688664Microsource (Natural product library)282.290662.80804
SGTC_9521185-0122232 μM0.285714678600ChemDiv (Drug-like library)282.360163.8214
SGTC_21986589944200 μM0.2833335290268Chembridge (Fragment library)292.333521.73925
SGTC_328k915-012063.5 μM0.2727276624543ChemDiv (Drug-like library)388.458964.48825TSC3-RPN4
SGTC_3148909789349.47 μM0.27272717174100Chembridge (Drug-like library)322.357823.63114
SGTC_14504112-426037.6 μM0.2686571633175ChemDiv (Drug-like library)335.44274.06714calcium & mitochondrial duress
SGTC_20214019055173 μM0.26415144827708Chembridge (Fragment library)163.216321.26112endomembrane recycling
SGTC_5433844-1095191 μM0.2615384230703ChemDiv (Drug-like library)354.399682.70205ERG2
SGTC_26222-methoxyxanthone59.94 μM0.25454571034Microsource (Natural product library)226.22742.94803