0871-0068

6-octylpyrrolo[3,4-b]pyrazine-5,7-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1276
Screen concentration 94.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 3414906
SMILES CCCCCCCCN1C(=O)C2=NC=CN=C2C1=O
Standardized SMILES CCCCCCCCN1C(=O)c2nccnc2C1=O
Molecular weight 261.3196
ALogP 2.57
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 36.16
% growth inhibition (Hom. pool) 9.73


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3414906
Download HIP data (tab-delimited text)  (excel)
Gene:ERG9(YHR190W)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.02||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:EXO84(YBR102C)|FD-Score:3.9|P-value:4.72E-5|Clearance:0.1||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GCD2(YGR083C)|FD-Score:3.97|P-value:3.55E-5|Clearance:0.07||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HCA4(YJL033W)|FD-Score:4.53|P-value:2.94E-6|Clearance:0.14||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:HEM2(YGL040C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.06||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:KRS1(YDR037W)|FD-Score:3.8|P-value:7.23E-5|Clearance:0.23||SGD DESC:Lysyl-tRNA synthetase Gene:MCD4(YKL165C)|FD-Score:5.25|P-value:7.59E-8|Clearance:0.72||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM3(YEL032W)|FD-Score:-4.2|P-value:1.34E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MTR3(YGR158C)|FD-Score:4.27|P-value:9.75E-6|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NUP49(YGL172W)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.23||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:QNS1(YHR074W)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RRP36(YOR287C)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:SMC5(YOL034W)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SMT3(YDR510W)|FD-Score:4.39|P-value:5.70E-6|Clearance:0.12||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPN1(YPR133C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.09||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:YDR187C(YDR187C_d)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.34||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR114C(YGR114C_d)|FD-Score:3.22|P-value:6.47E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YML6(YML025C)|FD-Score:-5.84|P-value:2.66E-9|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:ERG9(YHR190W)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.02||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:EXO84(YBR102C)|FD-Score:3.9|P-value:4.72E-5|Clearance:0.1||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GCD2(YGR083C)|FD-Score:3.97|P-value:3.55E-5|Clearance:0.07||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HCA4(YJL033W)|FD-Score:4.53|P-value:2.94E-6|Clearance:0.14||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:HEM2(YGL040C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.06||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:KRS1(YDR037W)|FD-Score:3.8|P-value:7.23E-5|Clearance:0.23||SGD DESC:Lysyl-tRNA synthetase Gene:MCD4(YKL165C)|FD-Score:5.25|P-value:7.59E-8|Clearance:0.72||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM3(YEL032W)|FD-Score:-4.2|P-value:1.34E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MTR3(YGR158C)|FD-Score:4.27|P-value:9.75E-6|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NUP49(YGL172W)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.23||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:QNS1(YHR074W)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RRP36(YOR287C)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:SMC5(YOL034W)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SMT3(YDR510W)|FD-Score:4.39|P-value:5.70E-6|Clearance:0.12||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPN1(YPR133C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.09||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:YDR187C(YDR187C_d)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.34||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR114C(YGR114C_d)|FD-Score:3.22|P-value:6.47E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YML6(YML025C)|FD-Score:-5.84|P-value:2.66E-9|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3414906
Download HOP data (tab-delimited text)  (excel)
Gene:AIM36(YMR157C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ARO1(YDR127W)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP5(YNL059C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ASR1(YPR093C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG11(YPR049C)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BUD16(YEL029C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:COG5(YNL051W)|FD-Score:5.77|P-value:3.94E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ6(YGR255C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:CPR4(YCR069W)|FD-Score:4.78|P-value:8.63E-7||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CTK3(YML112W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCS2(YOR173W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:DIA3(YDL024C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ERG28(YER044C)|FD-Score:-7.52|P-value:2.75E-14||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:ESC1(YMR219W)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FCY1(YPR062W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FHN1(YGR131W)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FLO1(YAR050W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FRA1(YLL029W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GRR1(YJR090C)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTT3(YEL017W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:IPT1(YDR072C)|FD-Score:4.38|P-value:6.04E-6||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:JIP3(YLR331C_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LNP1(YHR192W_p)|FD-Score:-4.36|P-value:6.52E-6||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LYS1(YIR034C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MCK1(YNL307C)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MON2(YNL297C)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRP10(YDL045W-A)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRP4(YHL004W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL51(YPR100W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSY1(YPL097W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NVJ1(YHR195W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PEP7(YDR323C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PTC7(YHR076W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PUT2(YHR037W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD18(YCR066W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RDL1(YOR285W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RPL22B(YFL034C-A)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPL39(YJL189W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPP2A(YOL039W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS23B(YPR132W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RSA1(YPL193W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RTF1(YGL244W)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT107(YHR154W)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SHH3(YMR118C_p)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SNC1(YAL030W)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNO2(YNL334C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SOV1(YMR066W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SPT2(YER161C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:TSC3(YBR058C-A)|FD-Score:9.22|P-value:1.46E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUM1(YOR251C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:URA1(YKL216W)|FD-Score:4.94|P-value:3.84E-7||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VTH1(YIL173W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YAR029W(YAR029W_p)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YAR1(YPL239W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR178W(YBR178W_d)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR023C(YCR023C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:7.04|P-value:9.47E-13||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR274C(YDR274C_d)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function Gene:YJR011C(YJR011C_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YLR361C-A(YLR361C-A_p)|FD-Score:6.45|P-value:5.72E-11||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function Gene:YOL107W(YOL107W_p)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:AIM36(YMR157C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ARO1(YDR127W)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP5(YNL059C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ASR1(YPR093C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG11(YPR049C)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BUD16(YEL029C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:COG5(YNL051W)|FD-Score:5.77|P-value:3.94E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ6(YGR255C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:CPR4(YCR069W)|FD-Score:4.78|P-value:8.63E-7||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CTK3(YML112W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCS2(YOR173W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:DIA3(YDL024C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ERG28(YER044C)|FD-Score:-7.52|P-value:2.75E-14||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:ESC1(YMR219W)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FCY1(YPR062W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FHN1(YGR131W)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FLO1(YAR050W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FRA1(YLL029W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GRR1(YJR090C)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTT3(YEL017W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:IPT1(YDR072C)|FD-Score:4.38|P-value:6.04E-6||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:JIP3(YLR331C_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LNP1(YHR192W_p)|FD-Score:-4.36|P-value:6.52E-6||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LYS1(YIR034C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MCK1(YNL307C)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MON2(YNL297C)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRP10(YDL045W-A)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRP4(YHL004W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL51(YPR100W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSY1(YPL097W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NVJ1(YHR195W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PEP7(YDR323C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PTC7(YHR076W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PUT2(YHR037W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD18(YCR066W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RDL1(YOR285W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RPL22B(YFL034C-A)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPL39(YJL189W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPP2A(YOL039W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS23B(YPR132W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RSA1(YPL193W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RTF1(YGL244W)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT107(YHR154W)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SHH3(YMR118C_p)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SNC1(YAL030W)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNO2(YNL334C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SOV1(YMR066W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SPT2(YER161C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:TSC3(YBR058C-A)|FD-Score:9.22|P-value:1.46E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUM1(YOR251C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:URA1(YKL216W)|FD-Score:4.94|P-value:3.84E-7||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VTH1(YIL173W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YAR029W(YAR029W_p)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YAR1(YPL239W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR178W(YBR178W_d)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR023C(YCR023C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:7.04|P-value:9.47E-13||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR274C(YDR274C_d)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function Gene:YJR011C(YJR011C_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YLR361C-A(YLR361C-A_p)|FD-Score:6.45|P-value:5.72E-11||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function Gene:YOL107W(YOL107W_p)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL165C5.257.59E-80.72MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YJL033W4.532.94E-60.14HCA4DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
YDR510W4.395.70E-60.12SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YGR158C4.279.75E-60.07MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YGL172W4.201.33E-50.23NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YGR083C3.973.55E-50.07GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YBR102C3.904.72E-50.10EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YDR037W3.807.23E-50.23KRS1Lysyl-tRNA synthetase
YDR187C_d3.571.76E-40.34YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YHR190W3.236.12E-40.02ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YGR114C_d3.226.47E-40.01YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YPR133C3.216.71E-40.09SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YGL040C3.119.30E-40.06HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YML092C3.050.001130.12PRE8Alpha 2 subunit of the 20S proteasome
YMR028W2.930.001700.00TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A9.221.46E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR003W-A_p7.049.47E-13YDR003W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YLR361C-A_p6.455.72E-11YLR361C-A_pPutative protein of unknown function
YNL051W5.773.94E-9COG5Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YJL189W5.012.72E-7RPL39Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
YLR434C_d4.963.61E-7YLR434C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W
YKL216W4.943.84E-7URA1Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
YCR069W4.788.63E-7CPR4Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
YPL193W4.731.12E-6RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YGR255C4.671.48E-6COQ6Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
YHR154W4.671.53E-6RTT107Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
YNL059C4.631.85E-6ARP5Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YHR076W4.444.42E-6PTC7Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope
YDR072C4.386.04E-6IPT1Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
YIR034C4.151.66E-5LYS1Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity

GO enrichment analysis for SGTC_1276
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2021.67E-55SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0357143TSC3-RPN4
0.1976.49E-53SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0526316TSC3-RPN4
0.1942.58E-51SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0819672TSC3-RPN4
0.1887.61E-48SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0869565TSC3-RPN4
0.1862.36E-47SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.114754TSC3-RPN4
0.1851.96E-46SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0862069TSC3-RPN4
0.1797.64E-44SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0526316TSC3-RPN4
0.1783.67E-43SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0980392TSC3-RPN4
0.1734.45E-41SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0535714TSC3-RPN4
0.1701.91E-39SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.130435TSC3-RPN4
0.1692.80E-39SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.0634921TSC3-RPN4
0.1672.58E-38SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0701754TSC3-RPN4
0.1627.02E-36SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.137255TSC3-RPN4
0.1605.55E-35SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.1TSC3-RPN4
0.1581.90E-34SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0740741TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1675st01848524.5 μM0.4444443323024TimTec (Natural product derivative library)306.403243.5090360S ribosome export
SGTC_5931203-021861.7 μM0.3863643666956ChemDiv (Drug-like library)244.33213.65302
SGTC_1000086-012849.59 μM0.35555693297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress
SGTC_1676st01848923.4 μM0.3404263696235TimTec (Natural product derivative library)306.403243.5090360S ribosome export
SGTC_711k292-0785179 μM0.31254146315ChemDiv (Drug-like library)240.345083.42413DNA intercalators
SGTC_24465162541200 μM0.2830191549321Miscellaneous267.367123.77104
SGTC_6660272-0154136 μM0.2777786870312ChemDiv (Drug-like library)276.357342.41523cell wall
SGTC_7313966-0296387 μM0.272727660956ChemDiv (Drug-like library)318.367582.44314
SGTC_13141222-001041.1 μM0.2653063526027ChemDiv (Drug-like library)251.321443.98514
SGTC_392N-ethylmaleimide8.33 μM0.2647064362Miscellaneous125.125280.1602RNA pol III & RNase P/MRP