0873-0036

5-[(Z)-N-[[4,6-di(piperidin-1-yl)-1,3,5-triazin-2-yl]amino]-C-methylcarbonimidoyl]benzene-1,3-diol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1277
Screen concentration 22.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5965735
SMILES CC(=NNC1=NC(=NC(=N1)N2CCCCC2)N3CCCCC3)C4=CC(=CC(=C4)O)O
Standardized SMILES CC(=NNc1nc(nc(n1)N2CCCCC2)N3CCCCC3)c4cc(O)cc(O)c4
Molecular weight 411.5007
ALogP 4.78
H-bond donor count 3
H-bond acceptor count 9
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.63
% growth inhibition (Hom. pool) 9.73


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5965735
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:3.93|P-value:4.23E-5|Clearance:0.31||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FAS1(YKL182W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.36||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GCR1(YPL075W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.18||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:NAM9(YNL137C)|FD-Score:-3.19|P-value:7.11E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:SHQ1(YIL104C)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.21||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:YGR073C(YGR073C_d)|FD-Score:5.62|P-value:9.80E-9|Clearance:1.44||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YJR023C(YJR023C_d)|FD-Score:3.97|P-value:3.54E-5|Clearance:0.04||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:CCT2(YIL142W)|FD-Score:3.93|P-value:4.23E-5|Clearance:0.31||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FAS1(YKL182W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.36||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GCR1(YPL075W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.18||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:NAM9(YNL137C)|FD-Score:-3.19|P-value:7.11E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:SHQ1(YIL104C)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.21||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:YGR073C(YGR073C_d)|FD-Score:5.62|P-value:9.80E-9|Clearance:1.44||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YJR023C(YJR023C_d)|FD-Score:3.97|P-value:3.54E-5|Clearance:0.04||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5965735
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM26(YKL037W)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM9(YER080W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARG1(YOL058W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:CHS3(YBR023C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COS111(YBR203W)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSF1(YLR087C)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DBF2(YGR092W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DOA1(YKL213C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DTR1(YBR180W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EFT1(YOR133W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HOF1(YMR032W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IAH1(YOR126C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IDP2(YLR174W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IML3(YBR107C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:KIP2(YPL155C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:LCL1(YPL056C_p)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB7(YBL006C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LEE1(YPL054W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LEO1(YOR123C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LYS4(YDR234W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MCX1(YBR227C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MOG1(YJR074W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSB2(YGR014W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:NUP170(YBL079W)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:17.1|P-value:6.07E-66||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP3(YLR148W)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX7(YDR142C)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PFA3(YNL326C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PGD1(YGL025C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PTR2(YKR093W)|FD-Score:4.32|P-value:7.70E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QRI5(YLR204W)|FD-Score:6.93|P-value:2.10E-12||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RNH1(YMR234W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RNY1(YPL123C)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:ROD1(YOR018W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAK1(YER129W)|FD-Score:5.77|P-value:3.86E-9||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SGE1(YPR198W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHE1(YBL031W)|FD-Score:4.88|P-value:5.37E-7||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIF2(YBR103W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SPC1(YJR010C-A)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPS2(YDR522C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SPT3(YDR392W)|FD-Score:6.61|P-value:1.96E-11||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:3.85|P-value:6.03E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SUR4(YLR372W)|FD-Score:6.64|P-value:1.57E-11||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SXM1(YDR395W)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:THI73(YLR004C)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:TRM1(YDR120C)|FD-Score:5.23|P-value:8.26E-8||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM7(YBR061C)|FD-Score:3.09|P-value:0.00100||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSA2(YDR453C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:5.82|P-value:2.86E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA2(YBR006W)|FD-Score:4.83|P-value:6.73E-7||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VAC7(YNL054W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS21(YOR089C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAL016C-B(YAL016C-B_d)|FD-Score:6.54|P-value:3.08E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDC1(YPL087W)|FD-Score:5.62|P-value:9.81E-9||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDR391C(YDR391C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER085C(YER085C_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function Gene:YMR018W(YMR018W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR031W-A(YMR031W-A_d)|FD-Score:4.34|P-value:7.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Putative protein of unknown function Gene:YNR068C(YNR068C_p)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YPR123C(YPR123C_d)|FD-Score:4.84|P-value:6.64E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP1(YHR155W)|FD-Score:4.38|P-value:5.93E-6||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YSP2(YDR326C)|FD-Score:6.68|P-value:1.16E-11||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADO1(YJR105W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM26(YKL037W)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM9(YER080W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARG1(YOL058W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:CHS3(YBR023C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COS111(YBR203W)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSF1(YLR087C)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DBF2(YGR092W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DOA1(YKL213C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DTR1(YBR180W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EFT1(YOR133W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HOF1(YMR032W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IAH1(YOR126C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IDP2(YLR174W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IML3(YBR107C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:KIP2(YPL155C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:LCL1(YPL056C_p)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB7(YBL006C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LEE1(YPL054W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LEO1(YOR123C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LYS4(YDR234W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MCX1(YBR227C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MOG1(YJR074W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSB2(YGR014W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:NUP170(YBL079W)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:17.1|P-value:6.07E-66||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP3(YLR148W)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX7(YDR142C)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PFA3(YNL326C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PGD1(YGL025C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PTR2(YKR093W)|FD-Score:4.32|P-value:7.70E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QRI5(YLR204W)|FD-Score:6.93|P-value:2.10E-12||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RNH1(YMR234W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RNY1(YPL123C)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:ROD1(YOR018W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAK1(YER129W)|FD-Score:5.77|P-value:3.86E-9||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SGE1(YPR198W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHE1(YBL031W)|FD-Score:4.88|P-value:5.37E-7||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIF2(YBR103W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SPC1(YJR010C-A)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPS2(YDR522C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SPT3(YDR392W)|FD-Score:6.61|P-value:1.96E-11||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:3.85|P-value:6.03E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SUR4(YLR372W)|FD-Score:6.64|P-value:1.57E-11||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SXM1(YDR395W)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:THI73(YLR004C)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:TRM1(YDR120C)|FD-Score:5.23|P-value:8.26E-8||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM7(YBR061C)|FD-Score:3.09|P-value:0.00100||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSA2(YDR453C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:5.82|P-value:2.86E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA2(YBR006W)|FD-Score:4.83|P-value:6.73E-7||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VAC7(YNL054W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS21(YOR089C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAL016C-B(YAL016C-B_d)|FD-Score:6.54|P-value:3.08E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDC1(YPL087W)|FD-Score:5.62|P-value:9.81E-9||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDR391C(YDR391C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER085C(YER085C_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function Gene:YMR018W(YMR018W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR031W-A(YMR031W-A_d)|FD-Score:4.34|P-value:7.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Putative protein of unknown function Gene:YNR068C(YNR068C_p)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YPR123C(YPR123C_d)|FD-Score:4.84|P-value:6.64E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP1(YHR155W)|FD-Score:4.38|P-value:5.93E-6||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YSP2(YDR326C)|FD-Score:6.68|P-value:1.16E-11||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR073C_d5.629.80E-91.44YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YIL104C4.181.47E-50.20SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YJR023C_d3.973.54E-50.04YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YIL142W3.934.23E-50.31CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YKL182W3.621.48E-40.36FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YPL075W3.255.69E-40.18GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YJR112W3.070.001050.09NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YGR218W2.990.001410.07CRM1Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
YOR250C2.920.001780.18CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YDL196W_d2.740.003080.06YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YHR197W2.680.003710.08RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YGL120C2.600.004650.00PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YJL061W2.600.004670.02NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YJL173C2.580.005010.10RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YIL004C2.480.006570.04BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C17.106.07E-66PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YLR204W6.932.10E-12QRI5Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
YDR326C6.681.16E-11YSP2Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
YLR372W6.641.57E-11SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YDR392W6.611.96E-11SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YAL016C-B_d6.543.08E-11YAL016C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YBR058C-A5.822.86E-9TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YER129W5.773.86E-9SAK1Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
YPL087W5.629.81E-9YDC1Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication
YLR148W5.511.81E-8PEP3Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
YBR203W5.501.88E-8COS111Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR120C5.238.26E-8TRM1tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
YBL031W4.885.37E-7SHE1Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
YPR123C_d4.846.64E-7YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YBR006W4.836.73E-7UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm

GO enrichment analysis for SGTC_1277
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3557.17E-175SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0786517PDR1
0.3325.90E-152SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0645161PDR1
0.3161.22E-136SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0684932PDR1
0.3109.04E-132SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.0735294sphingolipid biosynthesis & PDR1
0.2965.01E-120SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0985916sphingolipid biosynthesis & PDR1
0.2922.60E-116SGTC_1941st076221 61.8 μMTimTec (Natural product derivative library)28285680.0857143sphingolipid biosynthesis & PDR1
0.2801.87E-106SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.1PDR1
0.2594.27E-91SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.142857PDR1
0.2576.20E-90SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.119048PDR1
0.2571.53E-89SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0694444
0.2462.67E-82SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0810811PDR1
0.2331.93E-73SGTC_683panaxydol 45.0 μMMiscellaneous52832800.0266667sphingolipid biosynthesis & PDR1
0.2311.60E-72SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.0641026
0.2247.09E-68SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0895522TSC3-RPN4
0.2231.23E-67SGTC_8041595-0054 20.9 μMChemDiv (Drug-like library)7615230.0724638sphingolipid biosynthesis & PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_11961588-0294184 μM0.3859656144269ChemDiv (Drug-like library)355.4125834.57918
SGTC_8840851-0618334 μM0.359642473ChemDiv (Drug-like library)479.318054.17818
SGTC_553k007-025645.7 μM0.2545456287000ChemDiv (Drug-like library)229.2528832.48414heme biosynthesis & mitochondrial translocase
SGTC_1025k007-073170 μM0.2456146256118ChemDiv (Drug-like library)272.2743233.26425
SGTC_384k007-01445.16 μM0.2424246824886ChemDiv (Drug-like library)303.743561.91234ERAD & cell cycle
SGTC_1026k007-110530.6 μM0.2258067066794ChemDiv (Drug-like library)280.152543.60713ERAD & cell cycle
SGTC_1161k007-0610110 μM0.2258065843850ChemDiv (Drug-like library)296.3139664.74814ERAD & cell cycle
SGTC_3005907649671.43 μM0.22580621368410Chembridge (Drug-like library)310.390183.67713
SGTC_3268913768349.47 μM0.2258061953228Chembridge (Drug-like library)295.382143.15404
SGTC_3010908008971.43 μM0.21739117027233Chembridge (Drug-like library)378.2387833.49714