0874-0585

1-cyclohexyl-3-phenylthiourea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1278
Screen concentration 172.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 766306
SMILES C1CCC(CC1)NC(=S)NC2=CC=CC=C2
Standardized SMILES S=C(NC1CCCCC1)Nc2ccccc2
Molecular weight 234.3604
ALogP 4.37
H-bond donor count 2
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.83
% growth inhibition (Hom. pool) 5.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 766306
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:-3.23|P-value:6.08E-4|Clearance:0||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ATM1(YMR301C)|FD-Score:3.8|P-value:7.19E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:ATP16(YDL004W)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.03||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CAK1(YFL029C)|FD-Score:3.11|P-value:9.41E-4|Clearance:0.15||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:GUS1(YGL245W)|FD-Score:3.76|P-value:8.34E-5|Clearance:0||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:HSP10(YOR020C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.12||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:IRA1(YBR140C)|FD-Score:3.76|P-value:8.40E-5|Clearance:0.09||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NMT1(YLR195C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.01||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NRD1(YNL251C)|FD-Score:3.52|P-value:2.16E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PTI1(YGR156W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.06||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RFA3(YJL173C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.15||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RIO2(YNL207W)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.02||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RSC4(YKR008W)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC23(YPR181C)|FD-Score:3.82|P-value:6.64E-5|Clearance:0.02||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SPT16(YGL207W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.02||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:SRP1(YNL189W)|FD-Score:4.36|P-value:6.51E-6|Clearance:0.5||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TIM10(YHR005C-A)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.1||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:3.86|P-value:5.64E-5|Clearance:0.04||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:VTI1(YMR197C)|FD-Score:5.1|P-value:1.66E-7|Clearance:0.74||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:AAR2(YBL074C)|FD-Score:-3.23|P-value:6.08E-4|Clearance:0||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ATM1(YMR301C)|FD-Score:3.8|P-value:7.19E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:ATP16(YDL004W)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.03||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CAK1(YFL029C)|FD-Score:3.11|P-value:9.41E-4|Clearance:0.15||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:GUS1(YGL245W)|FD-Score:3.76|P-value:8.34E-5|Clearance:0||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:HSP10(YOR020C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.12||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:IRA1(YBR140C)|FD-Score:3.76|P-value:8.40E-5|Clearance:0.09||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NMT1(YLR195C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.01||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NRD1(YNL251C)|FD-Score:3.52|P-value:2.16E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PTI1(YGR156W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.06||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RFA3(YJL173C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.15||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RIO2(YNL207W)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.02||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RSC4(YKR008W)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SEC23(YPR181C)|FD-Score:3.82|P-value:6.64E-5|Clearance:0.02||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SPT16(YGL207W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.02||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:SRP1(YNL189W)|FD-Score:4.36|P-value:6.51E-6|Clearance:0.5||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TIM10(YHR005C-A)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.1||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:3.86|P-value:5.64E-5|Clearance:0.04||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:VTI1(YMR197C)|FD-Score:5.1|P-value:1.66E-7|Clearance:0.74||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 766306
Download HOP data (tab-delimited text)  (excel)
Gene:AIM9(YER080W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AKL1(YBR059C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:ALT1(YLR089C)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ARP8(YOR141C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG15(YCR068W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG33(YLR356W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:BSD2(YBR290W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CCC2(YDR270W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDC50(YCR094W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:FAR11(YNL127W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GPT2(YKR067W)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HOM2(YDR158W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HOR2(YER062C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication Gene:IDP1(YDL066W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC15(YPL017C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISY1(YJR050W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:LTE1(YAL024C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCH4(YOL119C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MIR1(YJR077C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MNN5(YJL186W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MZM1(YDR493W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NOT5(YPR072W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PET112(YBL080C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX19(YDL065C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:RCN2(YOR220W_p)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RDL1(YOR285W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RNR3(YIL066C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPL35B(YDL136W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSM24(YDR175C)|FD-Score:6.24|P-value:2.24E-10||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAS4(YDR181C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SER2(YGR208W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SIP4(YJL089W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SOD1(YJR104C)|FD-Score:4.96|P-value:3.44E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STF1(YDL130W-A)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SUR4(YLR372W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI4(YER111C)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAT1(YBR069C)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRM44(YPL030W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:UBA3(YPR066W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP6(YFR010W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VNX1(YNL321W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YBR178W(YBR178W_d)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function Gene:YDL124W(YDL124W)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YER079W(YER079W_p)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Putative protein of unknown function Gene:YGL149W(YGL149W_d)|FD-Score:-4.42|P-value:4.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR210C(YGR210C_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJL043W(YJL043W_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR062C(YNR062C_p)|FD-Score:5.2|P-value:9.77E-8||SGD DESC:Putative membrane protein of unknown function Gene:YOL106W(YOL106W_d)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR019W(YOR019W)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR053W(YOR053W_d)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR121C(YOR121C_d)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YVC1(YOR087W)|FD-Score:-4|P-value:3.14E-5||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:AIM9(YER080W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AKL1(YBR059C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:ALT1(YLR089C)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ARP8(YOR141C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG15(YCR068W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG33(YLR356W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:BSD2(YBR290W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CCC2(YDR270W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDC50(YCR094W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:FAR11(YNL127W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GPT2(YKR067W)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HOM2(YDR158W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HOR2(YER062C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication Gene:IDP1(YDL066W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC15(YPL017C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISY1(YJR050W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:LTE1(YAL024C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCH4(YOL119C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MIR1(YJR077C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MNN5(YJL186W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MZM1(YDR493W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NOT5(YPR072W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PET112(YBL080C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX19(YDL065C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:RCN2(YOR220W_p)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RDL1(YOR285W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RNR3(YIL066C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPL35B(YDL136W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSM24(YDR175C)|FD-Score:6.24|P-value:2.24E-10||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAS4(YDR181C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SER2(YGR208W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SIP4(YJL089W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SOD1(YJR104C)|FD-Score:4.96|P-value:3.44E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STF1(YDL130W-A)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SUR4(YLR372W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI4(YER111C)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAT1(YBR069C)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRM44(YPL030W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:UBA3(YPR066W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP6(YFR010W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VNX1(YNL321W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YBR178W(YBR178W_d)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR095W-A(YCR095W-A_p)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function Gene:YDL124W(YDL124W)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YER079W(YER079W_p)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Putative protein of unknown function Gene:YGL149W(YGL149W_d)|FD-Score:-4.42|P-value:4.89E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR210C(YGR210C_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJL043W(YJL043W_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR062C(YNR062C_p)|FD-Score:5.2|P-value:9.77E-8||SGD DESC:Putative membrane protein of unknown function Gene:YOL106W(YOL106W_d)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR019W(YOR019W)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR053W(YOR053W_d)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR121C(YOR121C_d)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YVC1(YOR087W)|FD-Score:-4|P-value:3.14E-5||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR197C5.101.66E-70.74VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YNL189W4.366.51E-60.50SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YJL143W3.865.64E-50.04TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YPR181C3.826.64E-50.02SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YMR301C3.807.19E-50.00ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YDL004W3.807.25E-50.03ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YGL245W3.768.34E-50.00GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YBR140C3.768.40E-50.09IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YLR195C3.671.19E-40.01NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YGL207W3.661.25E-40.02SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YOR020C3.641.35E-40.12HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YNL251C3.522.16E-40.00NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YJL173C3.522.18E-40.15RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YGR156W3.373.75E-40.06PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YKR008W3.314.60E-40.08RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR175C6.242.24E-10RSM24Mitochondrial ribosomal protein of the small subunit
YPL193W5.472.30E-8RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YNR062C_p5.209.77E-8YNR062C_pPutative membrane protein of unknown function
YBR069C5.171.20E-7TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YJR104C4.963.44E-7SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YER079W_p4.741.09E-6YER079W_pPutative protein of unknown function
YAL024C4.651.70E-6LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YER062C4.552.74E-6HOR2Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication
YER111C4.444.55E-6SWI4DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p
YBR290W4.251.06E-5BSD2Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
YDL136W4.211.27E-5RPL35BRibosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
YCR068W4.141.73E-5ATG15Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway
YNL321W4.102.06E-5VNX1Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate
YLR089C4.022.97E-5ALT1Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive
YOL106W_d3.826.57E-5YOL106W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1278
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0863.44E-11SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.0677966
0.0758.52E-9SGTC_20635246145 200.0 μMChembridge (Fragment library)7112400.16
0.0681.89E-7SGTC_31719103192 49.5 μMChembridge (Drug-like library)130184340.2
0.0647.55E-7SGTC_2636apigenin dimethyl ether 100.0 μMMicrosource (Natural product library)52816010.0483871
0.0622.01E-6SGTC_32539135987 49.5 μMChembridge (Drug-like library)41824300.12121260S ribosome export
0.0603.36E-6SGTC_29107948405 26.0 μMChembridge (Drug-like library)29713280.0833333plasma membrane duress
0.0552.60E-5SGTC_1025k007-0731 70.0 μMChemDiv (Drug-like library)62561180.111111
0.0535.05E-5SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.0545455
0.0535.27E-5SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.232143
0.0525.53E-5SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.138889TSC3-RPN4
0.0525.90E-5SGTC_1747st037496 66.6 μMTimTec (Natural product derivative library)7233050.111111
0.0526.63E-5SGTC_14103909-8106 77.9 μMChemDiv (Drug-like library)10392100.0821918
0.0527.19E-5SGTC_32649137442 49.5 μMChembridge (Drug-like library)170157260.309091
0.0518.28E-5SGTC_21145491910 81.2 μMChembridge (Fragment library)28492280.0677966
0.0519.97E-5SGTC_23819035592 200.0 μMChembridge (Fragment library)47496570.156863

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9031134-0042114 μM0.410256701009ChemDiv (Drug-like library)229.300843.75522
SGTC_23175986382200 μM0.333333877883Chembridge (Fragment library)229.27442.07913
SGTC_2955907625412.21 μM0.33333316459619Chembridge (Drug-like library)316.394762.72414
SGTC_1628st00425277.7 μM0.326531692968TimTec (Natural product derivative library)257.28452.4713
SGTC_13351486-0900202 μM0.315789X1335ChemDiv (Drug-like library)395.516254.1542
SGTC_1167k781-0610148 μM0.3137264525169ChemDiv (Drug-like library)276.314282.7934RNA processing & uracil transport
SGTC_3129912725749.47 μM0.3125837894Chembridge (Drug-like library)249.371763.61812
SGTC_11493909-796123.3 μM0.311111735261ChemDiv (Drug-like library)241.331464.0513
SGTC_22117117706200 μM0.309524822771Chembridge (Fragment library)177.199841.28522
SGTC_3264913744249.47 μM0.30909117015726Chembridge (Drug-like library)299.370842.94824