0906-2981

N'-[(2-oxonaphthalen-1-ylidene)methyl]thiophene-2-carbohydrazide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_128
Screen concentration 13.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 6740226
SMILES C1=CC=C2C(=C1)C=CC(=O)C2=CNNC(=O)C3=CC=CS3
Standardized SMILES Oc1ccc2ccccc2c1C=NNC(=O)c3cccs3
Molecular weight 296.3437
ALogP 2.01
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.71
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6740226
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.01||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ASA1(YPR085C)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.1||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CDC25(YLR310C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.07||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC31(YOR257W)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.07||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DBF4(YDR052C)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.05||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DBP9(YLR276C)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:ERG29(YMR134W_p)|FD-Score:3.88|P-value:5.28E-5|Clearance:0.01||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:EXO70(YJL085W)|FD-Score:3.77|P-value:8.31E-5|Clearance:0.18||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCR1(YPL075W)|FD-Score:7.99|P-value:6.90E-16|Clearance:2.15||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:LIP1(YMR298W)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.03||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MCM7(YBR202W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.05||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED8(YBR193C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP9(YJL010C)|FD-Score:-3.35|P-value:4.03E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PRP3(YDR473C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.4||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP4(YPR178W)|FD-Score:-3.34|P-value:4.18E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP40(YKL012W)|FD-Score:-4.08|P-value:2.24E-5|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex Gene:RFA3(YJL173C)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPT6(YGL048C)|FD-Score:5.04|P-value:2.36E-7|Clearance:0.38||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:SOF1(YLL011W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPC34(YKR037C)|FD-Score:-3.09|P-value:9.90E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SRP102(YKL154W)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:TAD3(YLR316C)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.06||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF10(YDR167W)|FD-Score:3.88|P-value:5.16E-5|Clearance:0.01||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAP42(YMR028W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:TEL2(YGR099W)|FD-Score:4.26|P-value:1.02E-5|Clearance:0.38||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFC4(YGR047C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.12||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TOM40(YMR203W)|FD-Score:5.84|P-value:2.59E-9|Clearance:0.8||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:ACP1(YKL192C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.01||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ASA1(YPR085C)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.1||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CDC25(YLR310C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.07||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC31(YOR257W)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.07||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DBF4(YDR052C)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.05||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DBP9(YLR276C)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:ERG29(YMR134W_p)|FD-Score:3.88|P-value:5.28E-5|Clearance:0.01||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:EXO70(YJL085W)|FD-Score:3.77|P-value:8.31E-5|Clearance:0.18||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCR1(YPL075W)|FD-Score:7.99|P-value:6.90E-16|Clearance:2.15||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:LIP1(YMR298W)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.03||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MCM7(YBR202W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.05||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED8(YBR193C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP9(YJL010C)|FD-Score:-3.35|P-value:4.03E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PRP3(YDR473C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.4||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP4(YPR178W)|FD-Score:-3.34|P-value:4.18E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP40(YKL012W)|FD-Score:-4.08|P-value:2.24E-5|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex Gene:RFA3(YJL173C)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPT6(YGL048C)|FD-Score:5.04|P-value:2.36E-7|Clearance:0.38||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:SOF1(YLL011W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPC34(YKR037C)|FD-Score:-3.09|P-value:9.90E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SRP102(YKL154W)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:TAD3(YLR316C)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.06||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF10(YDR167W)|FD-Score:3.88|P-value:5.16E-5|Clearance:0.01||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAP42(YMR028W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:TEL2(YGR099W)|FD-Score:4.26|P-value:1.02E-5|Clearance:0.38||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TFC4(YGR047C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.12||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TOM40(YMR203W)|FD-Score:5.84|P-value:2.59E-9|Clearance:0.8||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6740226
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AFT1(YGL071W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARR2(YPR200C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATS1(YAL020C)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BSP1(YPR171W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD16(YEL029C)|FD-Score:-3.73|P-value:9.61E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD17(YNR027W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BUD31(YCR063W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:COG6(YNL041C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIG1(YPL049C)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DSF2(YBR007C_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DYN2(YDR424C)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:FLC2(YAL053W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GEA1(YJR031C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GPB2(YAL056W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:HAP4(YKL109W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HUL4(YJR036C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:HXT8(YJL214W)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IRC4(YDR540C)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JID1(YPR061C)|FD-Score:-3.73|P-value:9.42E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:JNM1(YMR294W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LIF1(YGL090W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:MAC1(YMR021C)|FD-Score:4.89|P-value:5.02E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEP2(YNL142W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET8(YBR213W)|FD-Score:-4.68|P-value:1.45E-6||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MOT2(YER068W)|FD-Score:-3.75|P-value:8.80E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPS8(YMR158W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAT5(YOR253W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NTH2(YBR001C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUP2(YLR335W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:ODC1(YPL134C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:OMS1(YDR316W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PEP4(YPL154C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEX12(YMR026C)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PEX3(YDR329C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PMP2(YEL017C-A)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:RAX2(YLR084C)|FD-Score:7.25|P-value:2.04E-13||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:ROG3(YFR022W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:ROM1(YGR070W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPA49(YNL248C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPL8B(YLL045C)|FD-Score:-3.79|P-value:7.41E-5||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RRP6(YOR001W)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SDH1(YKL148C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEA4(YBL104C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SEG1(YMR086W)|FD-Score:4.52|P-value:3.08E-6||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SKG6(YHR149C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SNG1(YGR197C)|FD-Score:-4.93|P-value:4.09E-7||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SPL2(YHR136C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPO71(YDR104C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:SPS4(YOR313C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:YBR259W(YBR259W_p)|FD-Score:-5.75|P-value:4.53E-9||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCL049C(YCL049C_p)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YER010C(YER010C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function Gene:YGP1(YNL160W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR250C(YGR250C)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHR210C(YHR210C_p)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL032C(YIL032C_d)|FD-Score:5.65|P-value:7.87E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR036C(YLR036C_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR374C(YLR374C_d)|FD-Score:-4.76|P-value:9.48E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML002W(YML002W_p)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR160W(YMR160W_p)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNL144C(YNL144C_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:ZUO1(YGR285C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:ACM1(YPL267W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AFT1(YGL071W)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARR2(YPR200C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATS1(YAL020C)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BSP1(YPR171W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD16(YEL029C)|FD-Score:-3.73|P-value:9.61E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD17(YNR027W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BUD31(YCR063W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:COG6(YNL041C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIG1(YPL049C)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DSF2(YBR007C_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DYN2(YDR424C)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:FLC2(YAL053W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GEA1(YJR031C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GPB2(YAL056W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:HAP4(YKL109W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HUL4(YJR036C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:HXT8(YJL214W)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IRC4(YDR540C)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JID1(YPR061C)|FD-Score:-3.73|P-value:9.42E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:JNM1(YMR294W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LIF1(YGL090W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:MAC1(YMR021C)|FD-Score:4.89|P-value:5.02E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEP2(YNL142W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET8(YBR213W)|FD-Score:-4.68|P-value:1.45E-6||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MOT2(YER068W)|FD-Score:-3.75|P-value:8.80E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPS8(YMR158W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAT5(YOR253W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NTH2(YBR001C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUP2(YLR335W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:ODC1(YPL134C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:OMS1(YDR316W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PEP4(YPL154C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEX12(YMR026C)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PEX3(YDR329C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PMP2(YEL017C-A)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:RAX2(YLR084C)|FD-Score:7.25|P-value:2.04E-13||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:ROG3(YFR022W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:ROM1(YGR070W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPA49(YNL248C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPL8B(YLL045C)|FD-Score:-3.79|P-value:7.41E-5||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RRP6(YOR001W)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SDH1(YKL148C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEA4(YBL104C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SEG1(YMR086W)|FD-Score:4.52|P-value:3.08E-6||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SKG6(YHR149C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SNG1(YGR197C)|FD-Score:-4.93|P-value:4.09E-7||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SPL2(YHR136C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPO71(YDR104C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:SPS4(YOR313C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:YBR259W(YBR259W_p)|FD-Score:-5.75|P-value:4.53E-9||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCL049C(YCL049C_p)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YER010C(YER010C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function Gene:YGP1(YNL160W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR250C(YGR250C)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHR210C(YHR210C_p)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL032C(YIL032C_d)|FD-Score:5.65|P-value:7.87E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR036C(YLR036C_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR374C(YLR374C_d)|FD-Score:-4.76|P-value:9.48E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML002W(YML002W_p)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR160W(YMR160W_p)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNL144C(YNL144C_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:ZUO1(YGR285C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPL075W7.996.90E-162.15GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YMR203W5.842.59E-90.80TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YGL048C5.042.36E-70.38RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YDR473C4.661.58E-60.40PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YGR099W4.261.02E-50.38TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YDR167W3.885.16E-50.01TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YMR134W_p3.885.28E-50.01ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YPR085C3.875.54E-50.10ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YJL085W3.778.31E-50.18EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBR193C3.591.67E-40.01MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YBR202W3.571.76E-40.05MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YLR316C3.532.10E-40.06TAD3Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
YJL173C3.472.59E-40.02RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YLL011W3.452.84E-40.03SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
YMR298W3.423.11E-40.03LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR084C7.252.04E-13RAX2N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
YIL032C_d5.657.87E-9YIL032C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR021C4.895.02E-7MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YPR171W4.731.10E-6BSP1Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton
YDR424C4.711.22E-6DYN2Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex
YPL267W4.711.25E-6ACM1Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein
YDR329C4.572.47E-6PEX3Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p
YMR086W4.523.08E-6SEG1Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes
YAL020C4.366.39E-6ATS1Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
YGR070W4.261.04E-5ROM1GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
YLR036C_p4.251.07E-5YLR036C_pPutative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein
YGL071W4.062.44E-5AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YMR026C4.052.51E-5PEX12C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
YBR007C_p4.042.65E-5DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBR001C4.042.67E-5NTH2Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_128
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1596.81E-35SGTC_1310141-0277 33.8 μMChemDiv (Drug-like library)15477710.142857
0.0903.85E-12SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.138889DNA intercalators
0.0871.70E-11SGTC_510ag-490 170.0 μMICCB bioactive library53287790.202899
0.0864.25E-11SGTC_162k030-0015 37.8 μMChemDiv (Drug-like library)18099200.047619iron homeostasis
0.0822.60E-10SGTC_1569carminic acid 40.6 μMTimTec (Pure natural product library)147490.0930233
0.0772.71E-9SGTC_2640pentamidine 10.4 μMMiscellaneous47350.0634921mitochondrial processes
0.0741.54E-8SGTC_1390099-0334 718.6 μMChemDiv (Drug-like library)976900.119403
0.0708.67E-8SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0493827heme biosynthesis & mitochondrial translocase
0.0654.95E-7SGTC_23985228213 48.8 μMMiscellaneous807710.047619iron homeostasis
0.0621.59E-6SGTC_20685226020 147.0 μMChembridge (Fragment library)18105090.0625
0.0597.07E-6SGTC_40960-0234 25.7 μMChemDiv (Drug-like library)68211120.179487Golgi
0.0571.10E-5SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.133333iron homeostasis
0.0561.50E-5SGTC_2586curcumin 80.0 μMICCB bioactive library66103320.146667iron homeostasis
0.0561.97E-5SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.142857iron homeostasis
0.0552.49E-5SGTC_24275545055 42.8 μMMiscellaneous53728580.151515

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8981000-084928.3 μM0.646751807ChemDiv (Drug-like library)356.374042.72334
SGTC_3740880-023182.3 μM0.524596833587ChemDiv (Drug-like library)382.43453.24523iron homeostasis
SGTC_12800880-025310.1 μM0.524596836607ChemDiv (Drug-like library)410.44462.91724
SGTC_11841269-1718139 μM0.4814815403821ChemDiv (Drug-like library)260.311582.6214
SGTC_6671498-104459 μM0.4629636739982ChemDiv (Drug-like library)291.300722.77924RPP1 & pyrimidine depletion
SGTC_7851270-011165.3 μM0.4464296741994ChemDiv (Drug-like library)307.300122.53735
SGTC_11413253-038952.6 μM0.4406785402031ChemDiv (Drug-like library)290.337562.60315
SGTC_6441498-119763.3 μM0.3859656741615ChemDiv (Drug-like library)291.343782.86623
SGTC_7181130-0073349 μM0.379316740020ChemDiv (Drug-like library)282.724343.20213
SGTC_12082323-234750.3 μM0.3787885412760ChemDiv (Drug-like library)346.405664.22724