0922-0125

2-[6-[4-(3-chlorophenyl)piperazin-1-yl]hexyl]isoindole-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1283
Screen concentration 16.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 3360884
SMILES C1CN(CCN1CCCCCCN2C(=O)C3=CC=CC=C3C2=O)C4=CC(=CC=C4)Cl
Standardized SMILES Clc1cccc(c1)N2CCN(CCCCCCN3C(=O)c4ccccc4C3=O)CC2
Molecular weight 425.951
ALogP 4.85
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 29.45
% growth inhibition (Hom. pool) 14.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3360884
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:-3.34|P-value:4.12E-4|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:CDC39(YCR093W)|FD-Score:5.64|P-value:8.45E-9|Clearance:1.21||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CKS1(YBR135W)|FD-Score:-3.13|P-value:8.71E-4|Clearance:0||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:CMD1(YBR109C)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:ESP1(YGR098C)|FD-Score:3.85|P-value:5.82E-5|Clearance:0.03||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FOL1(YNL256W)|FD-Score:-3.1|P-value:9.80E-4|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:OLE1(YGL055W)|FD-Score:3.82|P-value:6.59E-5|Clearance:0.63||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RPL25(YOL127W)|FD-Score:5.22|P-value:9.19E-8|Clearance:1.21||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-3.87|P-value:5.43E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN5(YDL147W)|FD-Score:3.1|P-value:9.55E-4|Clearance:0.17||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSC58(YLR033W)|FD-Score:-5.79|P-value:3.58E-9|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:-5|P-value:2.86E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPN1(YPR133C)|FD-Score:-3.67|P-value:1.21E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TFA1(YKL028W)|FD-Score:4|P-value:3.14E-5|Clearance:0.05||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFC1(YBR123C)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIF34(YMR146C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.09||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM54(YJL054W)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.1||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:ARB1(YER036C)|FD-Score:-3.34|P-value:4.12E-4|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:CDC39(YCR093W)|FD-Score:5.64|P-value:8.45E-9|Clearance:1.21||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CKS1(YBR135W)|FD-Score:-3.13|P-value:8.71E-4|Clearance:0||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:CMD1(YBR109C)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:ESP1(YGR098C)|FD-Score:3.85|P-value:5.82E-5|Clearance:0.03||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FOL1(YNL256W)|FD-Score:-3.1|P-value:9.80E-4|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:OLE1(YGL055W)|FD-Score:3.82|P-value:6.59E-5|Clearance:0.63||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RPL25(YOL127W)|FD-Score:5.22|P-value:9.19E-8|Clearance:1.21||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-3.87|P-value:5.43E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN5(YDL147W)|FD-Score:3.1|P-value:9.55E-4|Clearance:0.17||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSC58(YLR033W)|FD-Score:-5.79|P-value:3.58E-9|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:-5|P-value:2.86E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPN1(YPR133C)|FD-Score:-3.67|P-value:1.21E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TFA1(YKL028W)|FD-Score:4|P-value:3.14E-5|Clearance:0.05||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFC1(YBR123C)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIF34(YMR146C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.09||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM54(YJL054W)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.1||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3360884
Download HOP data (tab-delimited text)  (excel)
Gene:AGA1(YNR044W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds Gene:AHT1(YHR093W_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARF2(YDL137W)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARI1(YGL157W)|FD-Score:6.5|P-value:4.07E-11||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARR3(YPR201W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:AVL9(YLR114C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BUD16(YEL029C)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:COS111(YBR203W)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DSK2(YMR276W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:DSS1(YMR287C)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ERG2(YMR202W)|FD-Score:5.43|P-value:2.77E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FHN1(YGR131W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FRE4(YNR060W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FTR1(YER145C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FYV8(YGR196C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:5.81|P-value:3.19E-9||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPD1(YDL022W)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HMS2(YJR147W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HPC2(YBR215W)|FD-Score:7.93|P-value:1.07E-15||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:HSE1(YHL002W)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HSP104(YLL026W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:IRC14(YOR135C_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTR4(YBR199W)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LAT1(YNL071W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LSP1(YPL004C)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family Gene:MAL32(YBR299W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MFM1(YPL060W)|FD-Score:5.39|P-value:3.43E-8||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MMM1(YLL006W)|FD-Score:-3.76|P-value:8.63E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:OGG1(YML060W)|FD-Score:-3.36|P-value:3.97E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OM14(YBR230C)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPI8(YKR035C_d)|FD-Score:4.88|P-value:5.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OSH3(YHR073W)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR16(YNL231C)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:POX1(YGL205W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PPA2(YMR267W)|FD-Score:-4.42|P-value:4.86E-6||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PUS4(YNL292W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:PUS9(YDL036C)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:PUT2(YHR037W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RCY1(YJL204C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RPL6B(YLR448W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRT13(YER066W_p)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSM24(YDR175C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SEC22(YLR268W)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEH1(YGL100W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SLS1(YLR139C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SOD2(YHR008C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SYO1(YDL063C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMS1(YDR105C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TOS3(YGL179C)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:VBA4(YDR119W_p)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VBA5(YKR105C_p)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VMA16(YHR026W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS15(YBR097W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS25(YJR102C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YBR296C-A(YBR296C-A_p)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL073W(YDL073W_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL114W(YDL114W_p)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YDR374C(YDR374C_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function Gene:YGL117W(YGL117W_p)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative protein of unknown function Gene:YGR042W(YGR042W_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR137W(YGR137W_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL132W(YJL132W_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YJL169W(YJL169W_d)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR129C(YJR129C_p)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YML122C(YML122C_d)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR326C(YMR326C_d)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YOL114C(YOL114C_p)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YSP2(YDR326C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZRT1(YGL255W)|FD-Score:4.97|P-value:3.40E-7||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:AGA1(YNR044W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds Gene:AHT1(YHR093W_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARF2(YDL137W)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARI1(YGL157W)|FD-Score:6.5|P-value:4.07E-11||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARR3(YPR201W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:AVL9(YLR114C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BUD16(YEL029C)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:COS111(YBR203W)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DSK2(YMR276W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:DSS1(YMR287C)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ERG2(YMR202W)|FD-Score:5.43|P-value:2.77E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FHN1(YGR131W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FRE4(YNR060W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FTR1(YER145C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FYV8(YGR196C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:5.81|P-value:3.19E-9||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPD1(YDL022W)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HMS2(YJR147W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HPC2(YBR215W)|FD-Score:7.93|P-value:1.07E-15||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:HSE1(YHL002W)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HSP104(YLL026W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:IRC14(YOR135C_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTR4(YBR199W)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LAT1(YNL071W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LSP1(YPL004C)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family Gene:MAL32(YBR299W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MFM1(YPL060W)|FD-Score:5.39|P-value:3.43E-8||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MMM1(YLL006W)|FD-Score:-3.76|P-value:8.63E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:OGG1(YML060W)|FD-Score:-3.36|P-value:3.97E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OM14(YBR230C)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPI8(YKR035C_d)|FD-Score:4.88|P-value:5.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OSH3(YHR073W)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR16(YNL231C)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:POX1(YGL205W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PPA2(YMR267W)|FD-Score:-4.42|P-value:4.86E-6||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PUS4(YNL292W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:PUS9(YDL036C)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:PUT2(YHR037W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RCY1(YJL204C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RPL6B(YLR448W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRT13(YER066W_p)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSM24(YDR175C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SEC22(YLR268W)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEH1(YGL100W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SLS1(YLR139C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SOD2(YHR008C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SYO1(YDL063C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMS1(YDR105C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TOS3(YGL179C)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:VBA4(YDR119W_p)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VBA5(YKR105C_p)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VMA16(YHR026W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS15(YBR097W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS25(YJR102C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YBR296C-A(YBR296C-A_p)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL073W(YDL073W_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL114W(YDL114W_p)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YDR374C(YDR374C_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function Gene:YGL117W(YGL117W_p)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative protein of unknown function Gene:YGR042W(YGR042W_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR137W(YGR137W_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL132W(YJL132W_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YJL169W(YJL169W_d)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR129C(YJR129C_p)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YML122C(YML122C_d)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR326C(YMR326C_d)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YOL114C(YOL114C_p)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YSP2(YDR326C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZRT1(YGL255W)|FD-Score:4.97|P-value:3.40E-7||SGD DESC:High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCR093W5.648.45E-91.21CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YOL127W5.229.19E-81.21RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YKL028W4.003.14E-50.05TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YJL054W3.953.85E-50.10TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YGR098C3.855.82E-50.03ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YGL055W3.826.59E-50.63OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YMR146C3.197.13E-40.09TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YDL147W3.109.55E-40.17RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YBR089W_d2.930.001670.09YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YJL195C_d2.840.002230.19YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YOL078W2.660.003940.04AVO1Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
YOL130W2.620.004380.03ALR1Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
YDR429C2.600.004730.03TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YDR118W2.560.005182.91E-5APC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
YGR267C2.560.005180.01FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR215W7.931.07E-15HPC2Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes
YGL157W6.504.07E-11ARI1NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
YFR009W5.813.19E-9GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YMR202W5.432.77E-8ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YPL060W5.393.43E-8MFM1Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
YGL255W4.973.40E-7ZRT1High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor
YKR035C_d4.885.24E-7OPI8_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
YDL063C4.751.01E-6SYO1Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YDR326C4.741.05E-6YSP2Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
YGL179C4.691.37E-6TOS3Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
YGL205W4.641.76E-6POX1Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
YLR268W4.631.87E-6SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YDL036C4.562.60E-6PUS9Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence
YHL002W4.405.42E-6HSE1Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
YDL137W4.366.41E-6ARF2ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1283
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1102.82E-17SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0714286ERG2
0.1088.28E-17SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0675676
0.0895.97E-12SGTC_1937st074714 47.2 μMTimTec (Natural product derivative library)172509700.154762
0.0898.40E-12SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0869565
0.0889.70E-12SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0895522ERG2
0.0848.90E-11SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0641026
0.0831.54E-10SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0533333
0.0832.08E-10SGTC_12410364-0022 50.8 μMChemDiv (Drug-like library)45638950.084507ERG2
0.0822.31E-10SGTC_31429086498 49.5 μMChembridge (Drug-like library)252363670.0864198iron homeostasis
0.0806.54E-10SGTC_2724maprotiline 69.6 μMMiscellaneous40110.119403ERG2
0.0791.19E-9SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0983607ERG2
0.0764.50E-9SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0384615calcium & mitochondrial duress
0.0766.15E-9SGTC_6513253-0966 34.4 μMChemDiv (Drug-like library)7814620.295082calcium & mitochondrial duress
0.0758.44E-9SGTC_22867945697 125.0 μMChembridge (Fragment library)12457330.171429
0.0741.11E-8SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.0813954

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1504k297-003330 μM0.4920634388117ChemDiv (Drug-like library)413.966764.69123
SGTC_3324913875960.75 μM0.39344345595710Chembridge (Drug-like library)375.333123.75624
SGTC_1065prochlorperazine maleate2.48 μM0.3606566436057NIH Clinical Collection490.01482-1.33428
SGTC_12630774-120864.8 μM0.333333906585ChemDiv (Drug-like library)304.342543.51612
SGTC_2859902864345.46 μM0.33333317151580Chembridge (Drug-like library)328.405462.51503
SGTC_3241913342149.47 μM0.33333317018221Chembridge (Drug-like library)428.935043.25205PDR1
SGTC_5154092-1064102 μM0.326923717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_1075aripiprazole15.2 μM0.31506860795Miscellaneous448.385384.99814
SGTC_1784092-080687.51 μM0.3090912169044ChemDiv (Drug-like library)328.87895.05102ergosterol depletion effects on membrane
SGTC_20875276107200 μM0.309091144405Chembridge (Fragment library)238.670241.51713