0929-0043

(5E)-5-[(4-fluorophenyl)methylidene]-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1284
Screen concentration 16.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1239883
SMILES C1=CC(=CC=C1C=C2C(=O)NC(=O)S2)F
Standardized SMILES Fc1ccc(C=C2SC(=O)NC2=O)cc1
Molecular weight 223.2235
ALogP 2.15
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.4
% growth inhibition (Hom. pool) 5.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1239883
Download HIP data (tab-delimited text)  (excel)
Gene:CKS1(YBR135W)|FD-Score:5.71|P-value:5.61E-9|Clearance:0.82||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:DAD2(YKR083C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FOL1(YNL256W)|FD-Score:-5.1|P-value:1.70E-7|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:IRR1(YIL026C)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.13||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KAR2(YJL034W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.14||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:NOG2(YNR053C)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NSL1(YPL233W)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.03||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUS1(YDL193W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PDS5(YMR076C)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.4||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:RER2(YBR002C)|FD-Score:5.04|P-value:2.31E-7|Clearance:0.82||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSC58(YLR033W)|FD-Score:-3.91|P-value:4.67E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:STU2(YLR045C)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.02||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TFC8(YPL007C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.36||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TIF6(YPR016C)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRS20(YBR254C)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.01||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:YPD1(YDL235C)|FD-Score:-3.39|P-value:3.48E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YSH1(YLR277C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.1||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:CKS1(YBR135W)|FD-Score:5.71|P-value:5.61E-9|Clearance:0.82||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:DAD2(YKR083C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FOL1(YNL256W)|FD-Score:-5.1|P-value:1.70E-7|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:IRR1(YIL026C)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.13||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KAR2(YJL034W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.14||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:NOG2(YNR053C)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NSL1(YPL233W)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.03||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUS1(YDL193W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PDS5(YMR076C)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.4||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:RER2(YBR002C)|FD-Score:5.04|P-value:2.31E-7|Clearance:0.82||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSC58(YLR033W)|FD-Score:-3.91|P-value:4.67E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:STU2(YLR045C)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.02||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TFC8(YPL007C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.36||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TIF6(YPR016C)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRS20(YBR254C)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.01||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:YPD1(YDL235C)|FD-Score:-3.39|P-value:3.48E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YSH1(YLR277C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.1||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1239883
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADE8(YDR408C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM9(YER080W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALT2(YDR111C_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:AMD1(YML035C)|FD-Score:6.61|P-value:1.90E-11||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ARO1(YDR127W)|FD-Score:6.76|P-value:6.77E-12||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG1(YGL180W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATG31(YDR022C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:BPH1(YCR032W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BRP1(YGL007W_d)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CAT5(YOR125C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CHO2(YGR157W)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CKB1(YGL019W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:DAL80(YKR034W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DSK2(YMR276W)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:DYN2(YDR424C)|FD-Score:5.41|P-value:3.21E-8||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ECM10(YEL030W)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ERG4(YGL012W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAT1(YBR041W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FHN1(YGR131W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FUN14(YAL008W)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GCN2(YDR283C)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:7.39|P-value:7.61E-14||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.26|P-value:7.06E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDS1(YOR355W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLE2(YER107C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPA2(YER020W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPB1(YOR371C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GRE3(YHR104W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HHT2(YNL031C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:IME2(YJL106W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:ISU1(YPL135W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KNS1(YLL019C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:KTR1(YOR099W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:LCB5(YLR260W)|FD-Score:4.54|P-value:2.84E-6||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MND2(YIR025W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRP10(YDL045W-A)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL17(YNL252C)|FD-Score:5.22|P-value:9.11E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:6.88|P-value:2.96E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OCA4(YCR095C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PEX5(YDR244W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PFK1(YGR240C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIM1(YBL022C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PPQ1(YPL179W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:RKM1(YPL208W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RMD8(YFR048W)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Cytosolic protein required for sporulation Gene:RPS29A(YLR388W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTR1(YER139C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SET3(YKR029C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SGF29(YCL010C)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SLA1(YBL007C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SPO23(YBR250W)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SRL4(YPL033C)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:TMA20(YER007C-A)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:6.72|P-value:8.94E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:6.11|P-value:4.83E-10||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBA4(YHR111W)|FD-Score:6.03|P-value:8.19E-10||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VMA7(YGR020C)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS17(YOR132W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YBR277C(YBR277C_d)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YDR008C(YDR008C_d)|FD-Score:10.8|P-value:1.16E-27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL101W(YGL101W_p)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YGL117W(YGL117W_p)|FD-Score:6.78|P-value:5.82E-12||SGD DESC:Putative protein of unknown function Gene:YIL012W(YIL012W_d)|FD-Score:4.83|P-value:6.81E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL169W(YJL169W_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKL151C(YKL151C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR118C(YLR118C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR363W-A(YLR363W-A_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YMR087W(YMR087W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL190W(YNL190W_p)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNR062C(YNR062C_p)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Putative membrane protein of unknown function Gene:ZIP2(YGL249W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:AAP1(YHR047C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADE8(YDR408C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM9(YER080W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALT2(YDR111C_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:AMD1(YML035C)|FD-Score:6.61|P-value:1.90E-11||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ARO1(YDR127W)|FD-Score:6.76|P-value:6.77E-12||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG1(YGL180W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATG31(YDR022C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:BPH1(YCR032W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BRP1(YGL007W_d)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CAT5(YOR125C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CHO2(YGR157W)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CKB1(YGL019W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:DAL80(YKR034W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DSK2(YMR276W)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:DYN2(YDR424C)|FD-Score:5.41|P-value:3.21E-8||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ECM10(YEL030W)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ERG4(YGL012W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAT1(YBR041W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FHN1(YGR131W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FUN14(YAL008W)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Mitochondrial protein of unknown function Gene:GCN2(YDR283C)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:7.39|P-value:7.61E-14||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.26|P-value:7.06E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDS1(YOR355W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLE2(YER107C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPA2(YER020W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPB1(YOR371C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GRE3(YHR104W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HHT2(YNL031C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:IME2(YJL106W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:ISU1(YPL135W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KNS1(YLL019C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:KTR1(YOR099W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:LCB5(YLR260W)|FD-Score:4.54|P-value:2.84E-6||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MND2(YIR025W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRP10(YDL045W-A)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL17(YNL252C)|FD-Score:5.22|P-value:9.11E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:6.88|P-value:2.96E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OCA4(YCR095C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PEX5(YDR244W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PFK1(YGR240C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIM1(YBL022C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PPQ1(YPL179W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:RKM1(YPL208W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RMD8(YFR048W)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Cytosolic protein required for sporulation Gene:RPS29A(YLR388W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTR1(YER139C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SET3(YKR029C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SGF29(YCL010C)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SLA1(YBL007C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SPO23(YBR250W)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SRL4(YPL033C)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:TMA20(YER007C-A)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:6.72|P-value:8.94E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:6.11|P-value:4.83E-10||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBA4(YHR111W)|FD-Score:6.03|P-value:8.19E-10||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VMA7(YGR020C)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS17(YOR132W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YBR277C(YBR277C_d)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YDR008C(YDR008C_d)|FD-Score:10.8|P-value:1.16E-27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL101W(YGL101W_p)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YGL117W(YGL117W_p)|FD-Score:6.78|P-value:5.82E-12||SGD DESC:Putative protein of unknown function Gene:YIL012W(YIL012W_d)|FD-Score:4.83|P-value:6.81E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL169W(YJL169W_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKL151C(YKL151C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR118C(YLR118C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR363W-A(YLR363W-A_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YMR087W(YMR087W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL190W(YNL190W_p)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNR062C(YNR062C_p)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Putative membrane protein of unknown function Gene:ZIP2(YGL249W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YBR135W5.715.61E-90.82CKS1Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters
YBR002C5.042.31E-70.82RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YPL007C4.221.20E-50.36TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YMR076C3.875.54E-50.40PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YKR083C3.462.69E-40.06DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YLR277C3.403.32E-40.10YSH1Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
YDL193W3.304.85E-40.00NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YBR254C3.304.87E-40.01TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
YPL233W3.294.99E-40.03NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YLR045C3.265.61E-40.02STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YJL034W3.246.03E-40.14KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YIL026C3.109.66E-40.13IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YPL153C2.970.001470.03RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YCR072C2.940.001630.05RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YHR074W2.890.001930.03QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d10.801.16E-27YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR026C7.397.61E-14GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YJL063C6.882.96E-12MRPL8Mitochondrial ribosomal protein of the large subunit
YGL117W_p6.785.82E-12YGL117W_pPutative protein of unknown function
YDR127W6.766.77E-12ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR007W6.728.94E-12TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YML035C6.611.90E-11AMD1AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
YGL026C6.114.83E-10TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YHR111W6.038.19E-10UBA4Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
YDR424C5.413.21E-8DYN2Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex
YEL009C5.267.06E-8GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YNL252C5.229.11E-8MRPL17Mitochondrial ribosomal protein of the large subunit
YIL012W_d4.836.81E-7YIL012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR276W4.562.59E-6DSK2Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
YLR260W4.542.84E-6LCB5Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules

GO enrichment analysis for SGTC_1284
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
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Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4111.80E-239SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0645161
0.4072.00E-234SGTC_18825615643 20.0 μMMiscellaneous22530750.322034TRP & mitochondrial translation
0.3679.03E-188SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.473684TRP & mitochondrial translation
0.3542.17E-174SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0701754
0.3514.54E-171SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.536585
0.3463.07E-165SGTC_18815652484 16.0 μMMiscellaneous22551290.184615TRP & mitochondrial translation
0.3388.18E-158SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.104478
0.3378.50E-157SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.55
0.3364.49E-156SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0645161
0.3323.32E-152SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0769231
0.3317.11E-151SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0540541
0.3265.28E-146SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0704225
0.3225.75E-142SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0253165
0.3216.59E-142SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.0615385
0.3182.09E-138SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.047619

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_205252651827.14 μM11239884Chembridge (Fragment library)223.2235432.14714
SGTC_8920929-007629 μM0.696975751038ChemDiv (Drug-like library)219.259662.42813TRP & mitochondrial translation
SGTC_380929-005158.27 μM0.6666671379719ChemDiv (Drug-like library)223.2235432.14714
SGTC_2165572719732.14 μM0.5675681361712Chembridge (Fragment library)211.26081.60414
SGTC_6770929-0063136 μM0.551539583ChemDiv (Drug-like library)265.285041.90915
SGTC_245553777129.75 μM0.5384622243908Miscellaneous219.259662.42813
SGTC_1697st02555427.74 μM0.5365851269519TimTec (Natural product derivative library)249.242581.7115
SGTC_440929-005561.21 μM0.4871791379590ChemDiv (Drug-like library)239.678142.60613
SGTC_2102537781672.76 μM0.4871791379702Chembridge (Fragment library)195.19521.33714
SGTC_18835-benzylidenerhodanine20 μM0.4736841273210Miscellaneous221.298682.84113TRP & mitochondrial translation