0929-0070

(5E)-5-[(2-hydroxy-3-methoxyphenyl)methylidene]-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1286
Screen concentration 131.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1420408
SMILES COC1=CC=CC(=C1O)C=C2C(=O)NC(=O)S2
Standardized SMILES COc1cccc(C=C2SC(=O)NC2=O)c1O
Molecular weight 251.2585
ALogP 1.68
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.67
% growth inhibition (Hom. pool) 5.07


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1420408
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:CRM1(YGR218W)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:ERG11(YHR007C)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.89||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:IQG1(YPL242C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.15||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAR2(YJL034W)|FD-Score:4.08|P-value:2.27E-5|Clearance:0.39||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KRE29(YER038C)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.13||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MCD4(YKL165C)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.05||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MTR10(YOR160W)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.03||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NMT1(YLR195C)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NOG2(YNR053C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NRD1(YNL251C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.3||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:POP6(YGR030C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPN12(YFR052W)|FD-Score:-3.11|P-value:9.29E-4|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RRB1(YMR131C)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RTT105(YER104W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC21(YNL287W)|FD-Score:5.17|P-value:1.18E-7|Clearance:0.89||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC4(YFL005W)|FD-Score:-3.89|P-value:4.96E-5|Clearance:0||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC5(YDR166C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.06||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SRP54(YPR088C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.04||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TFG1(YGR186W)|FD-Score:4.28|P-value:9.38E-6|Clearance:0.05||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:AAR2(YBL074C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:CRM1(YGR218W)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:ERG11(YHR007C)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.89||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:IQG1(YPL242C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.15||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAR2(YJL034W)|FD-Score:4.08|P-value:2.27E-5|Clearance:0.39||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KRE29(YER038C)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.13||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MCD4(YKL165C)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.05||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MTR10(YOR160W)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.03||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NMT1(YLR195C)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NOG2(YNR053C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NRD1(YNL251C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.3||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:POP6(YGR030C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPN12(YFR052W)|FD-Score:-3.11|P-value:9.29E-4|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RRB1(YMR131C)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RTT105(YER104W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC21(YNL287W)|FD-Score:5.17|P-value:1.18E-7|Clearance:0.89||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC4(YFL005W)|FD-Score:-3.89|P-value:4.96E-5|Clearance:0||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC5(YDR166C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.06||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SRP54(YPR088C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.04||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TFG1(YGR186W)|FD-Score:4.28|P-value:9.38E-6|Clearance:0.05||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1420408
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:4.92|P-value:4.42E-7||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:AFT1(YGL071W)|FD-Score:5.75|P-value:4.47E-9||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM6(YDL237W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APL6(YGR261C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APN1(YKL114C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARL1(YBR164C)|FD-Score:-4.11|P-value:2.01E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG29(YPL166W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:CHO1(YER026C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS3(YBR023C)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CPA1(YOR303W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:CTK3(YML112W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:FMP25(YLR077W)|FD-Score:-4.86|P-value:5.98E-7||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FMP40(YPL222W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCS1(YDL226C)|FD-Score:4.36|P-value:6.45E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GPT2(YKR067W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GUP1(YGL084C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HNT1(YDL125C)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:IRC24(YIR036C_p)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IXR1(YKL032C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LEU9(YOR108W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAF1(YDR005C)|FD-Score:5.53|P-value:1.65E-8||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MDJ1(YFL016C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MET13(YGL125W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MLS1(YNL117W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MON2(YNL297C)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NRT1(YOR071C)|FD-Score:5.31|P-value:5.60E-8||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:PAH1(YMR165C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PAU2(YEL049W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PHO4(YFR034C)|FD-Score:-4.67|P-value:1.54E-6||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO86(YJL117W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PKH3(YDR466W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMP1(YCR024C-A)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RAV2(YDR202C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPL24B(YGR148C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:6.76|P-value:7.08E-12||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:SGM1(YJR134C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLF1(YDR515W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLM2(YNL047C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SNL1(YIL016W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:STD1(YOR047C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STP3(YLR375W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:UBX6(YJL048C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VTC3(YPL019C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YBR242W(YBR242W_p)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDL041W(YDL041W_d)|FD-Score:6.34|P-value:1.18E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL185C(YGL185C_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR250C(YGR250C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHR097C(YHR097C_p)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YHR125W(YHR125W_d)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR140W(YHR140W_p)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YKR047W(YKR047W_d)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR125W(YLR125W_p)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR171W(YLR171W_d)|FD-Score:-3.74|P-value:9.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YLR428C(YLR428C_d)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR144W(YMR144W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL146C-A(YNL146C-A_p)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Putative protein of unknown function Gene:YNR061C(YNR061C_p)|FD-Score:-3.12|P-value:9.20E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOL037C(YOL037C_d)|FD-Score:6.84|P-value:4.02E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOL163W(YOL163W_p)|FD-Score:-4.62|P-value:1.91E-6||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YSP3(YOR003W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:AAC1(YMR056C)|FD-Score:4.92|P-value:4.42E-7||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:AFT1(YGL071W)|FD-Score:5.75|P-value:4.47E-9||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM6(YDL237W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APL6(YGR261C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APN1(YKL114C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARL1(YBR164C)|FD-Score:-4.11|P-value:2.01E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG29(YPL166W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:CHO1(YER026C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS3(YBR023C)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CPA1(YOR303W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:CTK3(YML112W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:FMP25(YLR077W)|FD-Score:-4.86|P-value:5.98E-7||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FMP40(YPL222W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCS1(YDL226C)|FD-Score:4.36|P-value:6.45E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GPT2(YKR067W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GUP1(YGL084C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HNT1(YDL125C)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:IRC24(YIR036C_p)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:IXR1(YKL032C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LEU9(YOR108W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAF1(YDR005C)|FD-Score:5.53|P-value:1.65E-8||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MDJ1(YFL016C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MET13(YGL125W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MLS1(YNL117W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MON2(YNL297C)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NRT1(YOR071C)|FD-Score:5.31|P-value:5.60E-8||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:PAH1(YMR165C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PAU2(YEL049W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PHO4(YFR034C)|FD-Score:-4.67|P-value:1.54E-6||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO86(YJL117W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PKH3(YDR466W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMP1(YCR024C-A)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RAV2(YDR202C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPL24B(YGR148C)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:6.76|P-value:7.08E-12||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:SGM1(YJR134C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLF1(YDR515W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLM2(YNL047C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SNL1(YIL016W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:STD1(YOR047C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STP3(YLR375W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:UBX6(YJL048C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VTC3(YPL019C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YBR242W(YBR242W_p)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDL041W(YDL041W_d)|FD-Score:6.34|P-value:1.18E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL185C(YGL185C_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR250C(YGR250C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHR097C(YHR097C_p)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YHR125W(YHR125W_d)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR140W(YHR140W_p)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YKR047W(YKR047W_d)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR125W(YLR125W_p)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR171W(YLR171W_d)|FD-Score:-3.74|P-value:9.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YLR428C(YLR428C_d)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR144W(YMR144W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL146C-A(YNL146C-A_p)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Putative protein of unknown function Gene:YNR061C(YNR061C_p)|FD-Score:-3.12|P-value:9.20E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOL037C(YOL037C_d)|FD-Score:6.84|P-value:4.02E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOL163W(YOL163W_p)|FD-Score:-4.62|P-value:1.91E-6||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YSP3(YOR003W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative precursor to the subtilisin-like protease III

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR007C5.181.09E-70.89ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YNL287W5.171.18E-70.89SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YGR186W4.289.38E-60.05TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YPL242C4.231.19E-50.15IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YJL034W4.082.27E-50.39KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YNL251C3.691.12E-40.29NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YER038C3.393.44E-40.13KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YER104W3.275.47E-40.00RTT105Protein with a role in regulation of Ty1 transposition
YKL165C3.265.51E-40.05MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YOR160W3.216.55E-40.03MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YDR166C3.197.23E-40.06SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YPR088C3.128.97E-40.04SRP54Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
YGL044C3.080.001040.07RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YBR202W3.010.001320.04MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YOR341W2.970.001510.14RPA190RNA polymerase I largest subunit A190

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL037C_d6.844.02E-12YOL037C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W
YJL191W6.767.08E-12RPS14BProtein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication
YDL041W_d6.341.18E-10YDL041W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
YBR023C5.981.10E-9CHS3Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
YOR106W5.971.20E-9VAM3Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
YGL071W5.754.47E-9AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YDR005C5.531.65E-8MAF1Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
YOR071C5.315.60E-8NRT1High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity
YLR375W5.052.22E-7STP3Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids
YMR056C4.924.42E-7AAC1Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
YHR125W_d4.731.13E-6YHR125W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR108W4.661.55E-6LEU9Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
YPL166W4.621.94E-6ATG29Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDL226C4.366.45E-6GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YGL084C4.171.55E-5GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport

GO enrichment analysis for SGTC_1286
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1195.16E-20SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.0675676fatty acid desaturase (OLE1)
0.0977.20E-14SGTC_577r071-0003 38.6 μMChemDiv (Drug-like library)45685170.107143fatty acid desaturase (OLE1)
0.0961.68E-13SGTC_14790549-0158 134.0 μMChemDiv (Drug-like library)54402020.138462
0.0945.91E-13SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.0895522fatty acid desaturase (OLE1)
0.0772.66E-9SGTC_13081187-1617 41.4 μMChemDiv (Drug-like library)28825700.119403fatty acid desaturase (OLE1)
0.0741.47E-8SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.0610687
0.0731.60E-8SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.0833333ERAD & cell cycle
0.0732.41E-8SGTC_245methotrexate 289.6 μMMiscellaneous1269410.0666667
0.0723.25E-8SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.1fatty acid desaturase (OLE1)
0.0707.45E-8SGTC_31559099265 49.5 μMChembridge (Drug-like library)252364990.0657895
0.0691.31E-7SGTC_2675theaflavin digallate 100.0 μMMicrosource (Natural product library)4673200.0909091iron homeostasis
0.0681.52E-7SGTC_7680384-0033 77.3 μMChemDiv (Drug-like library)39653050.0952381fatty acid desaturase (OLE1)
0.0672.37E-7SGTC_3730844-0104 25.9 μMChemDiv (Drug-like library)53758040.114754ERAD & cell cycle
0.0663.71E-7SGTC_2734clemastine 41.6 μMMiscellaneous269870.0821918ERG2
0.0664.49E-7SGTC_1550gossypolone 36.6 μMTimTec (Pure natural product library)54786220.131148

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8900929-000647.2 μM0.7674421378169ChemDiv (Drug-like library)267.324062.58325
SGTC_6770929-0063136 μM0.5434781539583ChemDiv (Drug-like library)265.285041.90915
SGTC_440929-005561.21 μM0.5227271379590ChemDiv (Drug-like library)239.678142.60613
SGTC_12850929-006965.5 μM0.4791671206237ChemDiv (Drug-like library)379.02463.19724
SGTC_245553777129.75 μM0.4680852243908Miscellaneous219.259662.42813
SGTC_8920929-007629 μM0.4444445751038ChemDiv (Drug-like library)219.259662.42813TRP & mitochondrial translation
SGTC_8910929-0073133 μM0.445603632ChemDiv (Drug-like library)244.269122.23523
SGTC_380929-005158.27 μM0.43751379719ChemDiv (Drug-like library)223.2235432.14714
SGTC_2102537781672.76 μM0.4255321379702Chembridge (Fragment library)195.19521.33714
SGTC_712r015-000980.6 μM0.4166676813872ChemDiv (Drug-like library)342.38898-0.25426