0973-0013

N-(4-nitrophenyl)-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1288
Screen concentration 65.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 4315430
SMILES C1CCC2=C(C1)C(=CS2)C(=O)NC3=CC=C(C=C3)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(NC(=O)c2csc3CCCCc23)cc1
Molecular weight 302.3483
ALogP 3.55
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.63
% growth inhibition (Hom. pool) 11.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4315430
Download HIP data (tab-delimited text)  (excel)
Gene:COF1(YLL050C)|FD-Score:-3.33|P-value:4.34E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:MAK5(YBR142W)|FD-Score:3.82|P-value:6.72E-5|Clearance:0.39||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:RIM2(YBR192W)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.7||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SUP35(YDR172W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.34||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:COF1(YLL050C)|FD-Score:-3.33|P-value:4.34E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:MAK5(YBR142W)|FD-Score:3.82|P-value:6.72E-5|Clearance:0.39||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:RIM2(YBR192W)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.7||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SUP35(YDR172W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.34||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4315430
Download HOP data (tab-delimited text)  (excel)
Gene:AIM29(YKR074W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIP1(YMR092C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ATG29(YPL166W)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BPH1(YCR032W)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BTS1(YPL069C)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CNL1(YDR357C_p)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:CPA2(YJR109C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:DDI1(YER143W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:FRA2(YGL220W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUR4(YBR021W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:6.03|P-value:8.42E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDT1(YBR187W_p)|FD-Score:4.8|P-value:7.77E-7||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GFD1(YMR255W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GRX2(YDR513W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HOL1(YNR055C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:IMP2'(YIL154C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LSC2(YGR244C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LTE1(YAL024C)|FD-Score:5.25|P-value:7.80E-8||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAF1(YDR005C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MCA1(YOR197W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MFM1(YPL060W)|FD-Score:5.47|P-value:2.31E-8||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MNN5(YJL186W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOG1(YJR074W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NCE101(YJL205C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NOT5(YPR072W)|FD-Score:-5.27|P-value:6.75E-8||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPC2(YDL046W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:NUM1(YDR150W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI9(YLR338W_d)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OST5(YGL226C-A)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAC10(YGR078C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAM17(YKR065C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PUS4(YNL292W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:PUS9(YDL036C)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:QRI7(YDL104C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RPS16B(YDL083C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTK1(YDL025C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SDL1(YIL167W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SGM1(YJR134C)|FD-Score:5.7|P-value:5.83E-9||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLM3(YDL033C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SSF1(YHR066W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:5.8|P-value:3.23E-9||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SUR4(YLR372W)|FD-Score:4.77|P-value:9.40E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TPN1(YGL186C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBA4(YHR111W)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:XBP1(YIL101C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAP1801(YHR161C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCK2(YNL154C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YER121W(YER121W_p)|FD-Score:3.78|P-value:7.77E-5||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YJL055W(YJL055W_p)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKR078W(YKR078W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR374C(YLR374C_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMC1(YPR058W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YMR001C-A(YMR001C-A_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL108C(YNL108C_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOR019W(YOR019W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPL071C(YPL071C_p)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:AIM29(YKR074W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIP1(YMR092C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ATG29(YPL166W)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BPH1(YCR032W)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BTS1(YPL069C)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CNL1(YDR357C_p)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:CPA2(YJR109C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:DDI1(YER143W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:FRA2(YGL220W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUR4(YBR021W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL11(YOL051W)|FD-Score:6.03|P-value:8.42E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDT1(YBR187W_p)|FD-Score:4.8|P-value:7.77E-7||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GFD1(YMR255W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GRX2(YDR513W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HOL1(YNR055C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:IMP2'(YIL154C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LSC2(YGR244C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LTE1(YAL024C)|FD-Score:5.25|P-value:7.80E-8||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAF1(YDR005C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MCA1(YOR197W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MFM1(YPL060W)|FD-Score:5.47|P-value:2.31E-8||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MNN5(YJL186W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOG1(YJR074W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NCE101(YJL205C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NOT5(YPR072W)|FD-Score:-5.27|P-value:6.75E-8||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPC2(YDL046W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:NUM1(YDR150W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI9(YLR338W_d)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OST5(YGL226C-A)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAC10(YGR078C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAM17(YKR065C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PUS4(YNL292W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:PUS9(YDL036C)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:QRI7(YDL104C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RPS16B(YDL083C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTK1(YDL025C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SDL1(YIL167W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SGM1(YJR134C)|FD-Score:5.7|P-value:5.83E-9||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLM3(YDL033C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SSF1(YHR066W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:5.8|P-value:3.23E-9||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SUR4(YLR372W)|FD-Score:4.77|P-value:9.40E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TPN1(YGL186C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBA4(YHR111W)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:XBP1(YIL101C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAP1801(YHR161C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCK2(YNL154C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YER121W(YER121W_p)|FD-Score:3.78|P-value:7.77E-5||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YJL055W(YJL055W_p)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKR078W(YKR078W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR374C(YLR374C_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMC1(YPR058W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YMR001C-A(YMR001C-A_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL108C(YNL108C_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOR019W(YOR019W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YPL071C(YPL071C_p)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR192W4.523.16E-60.70RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YBR142W3.826.72E-50.39MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YDR172W3.423.08E-40.34SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YNL131W3.080.001030.03TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YJL034W3.050.001150.01KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YOR257W3.040.001180.02CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YDR037W3.020.001250.02KRS1Lysyl-tRNA synthetase
YBR121C3.000.001340.07GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YHR072W2.940.001660.03ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YLR088W2.910.001810.04GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YBL105C2.870.002060.04PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YNR016C2.830.002340.03ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YNL262W2.800.002570.01POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YGL048C2.790.002630.14RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YPR085C2.650.004000.13ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL051W6.038.42E-10GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YNR031C5.803.23E-9SSK2MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
YJR134C5.705.83E-9SGM1Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus
YPL060W5.472.31E-8MFM1Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
YAL024C5.257.80E-8LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YMR001C-A_p5.151.31E-7YMR001C-A_pPutative protein of unknown function
YBR187W_p4.807.77E-7GDT1_pPutative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis
YLR372W4.779.40E-7SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YJR074W4.651.65E-6MOG1Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
YPL166W4.592.24E-6ATG29Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YIL167W4.221.25E-5SDL1Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase
YDL083C4.141.74E-5RPS16BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
YKR074W4.082.27E-5AIM29Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
YER074W4.032.76E-5RPS24AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
YGR244C3.904.89E-5LSC2Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate

GO enrichment analysis for SGTC_1288
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0814.31E-10SGTC_13071187-1599 12.2 μMChemDiv (Drug-like library)15384710.0757576PDR1
0.0806.19E-10SGTC_2512diallyl trisulfide 49.2 μMMicrosource (Natural product library)16315NA
0.0807.10E-10SGTC_5941487-1584 10.8 μMChemDiv (Drug-like library)50133320.235294
0.0808.78E-10SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.0547945RNA processing & uracil transport
0.0791.53E-9SGTC_1621st003705 32.4 μMTimTec (Natural product derivative library)242070320.0777778calcium & mitochondrial duress
0.0765.37E-9SGTC_2251lansoprazole 556.0 μMNIH Clinical Collection38830.0465116
0.0759.81E-9SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.0588235RNA processing & uracil transport
0.0741.12E-8SGTC_28699037858 32.5 μMChembridge (Drug-like library)271870420.185714
0.0723.17E-8SGTC_6010986-0029 62.9 μMChemDiv (Drug-like library)247479810.0857143calcium & mitochondrial duress
0.0714.31E-8SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.0895522RNA processing & uracil transport
0.0699.58E-8SGTC_7353076-0345 636.0 μMChemDiv (Drug-like library)28889900.0945946
0.0681.76E-7SGTC_2405ticlatone 3.5 μMMiscellaneous62580.0655738
0.0672.57E-7SGTC_1817st052116 64.4 μMTimTec (Natural product derivative library)43613980.0526316RNA processing & uracil transport
0.0663.37E-7SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0454545calcium & mitochondrial duress
0.0664.71E-7SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0192308calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_990109-0120622.8 μM0.44898759414ChemDiv (Drug-like library)321.126163.25713
SGTC_6260214-000933 μM0.3888893437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_850868-0259160.06 μM0.38461583173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_13281441-002569.2 μM0.3508774562601ChemDiv (Drug-like library)287.270722.2724
SGTC_7150868-0130184 μM0.343752876258ChemDiv (Drug-like library)439.441182.73627
SGTC_22727805186180 μM0.3333332956062Chembridge (Fragment library)268.335342.54524cell wall signaling
SGTC_12600700-16177.75 μM0.3207552853078ChemDiv (Drug-like library)235.235922.80914calcium & mitochondrial duress
SGTC_625k213-0058121 μM0.3188413655420ChemDiv (Drug-like library)476.868584.50426translation
SGTC_9133487-1245167 μM0.317464016153ChemDiv (Drug-like library)349.831844.53424
SGTC_21836420625130.51 μM0.3166672897619Chembridge (Fragment library)277.299043.17124mitochondrial processes