1013-0063

6-oxo-3-[[3-(trifluoromethyl)phenyl]hydrazinylidene]cyclohexa-1,4-diene-1-carbaldehyde

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1295
Screen concentration 9.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 6826038
SMILES C1=CC(=CC(=C1)NN=C2C=CC(=O)C(=C2)C=O)C(F)(F)F
Standardized SMILES Oc1ccc(cc1C=O)N=Nc2cccc(c2)C(F)(F)F
Molecular weight 294.2287
ALogP 3.1
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.92
% growth inhibition (Hom. pool) 6.04


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6826038
Download HIP data (tab-delimited text)  (excel)
Gene:ERG12(YMR208W)|FD-Score:-3.13|P-value:8.88E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:SEC3(YER008C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.7||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:-3.77|P-value:8.32E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ERG12(YMR208W)|FD-Score:-3.13|P-value:8.88E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:SEC3(YER008C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.7||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:-3.77|P-value:8.32E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6826038
Download HOP data (tab-delimited text)  (excel)
Gene:AAT1(YKL106W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ADE1(YAR015W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:APL1(YJR005W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATG14(YBR128C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:BST1(YFL025C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CLU1(YMR012W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX5A(YNL052W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:DIG1(YPL049C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DJP1(YIR004W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DYN3(YMR299C)|FD-Score:4.32|P-value:7.90E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FAR8(YMR029C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FET3(YMR058W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:GET2(YER083C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GOS1(YHL031C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HES1(YOR237W)|FD-Score:7.8|P-value:3.02E-15||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:IRC24(YIR036C_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:LYS14(YDR034C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MCR1(YKL150W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MDM36(YPR083W)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:NCL1(YBL024W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:PGC1(YPL206C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO81(YGR233C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL26B(YGR034W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:SAP1(YER047C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SAP30(YMR263W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAP4(YGL229C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SSF2(YDR312W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:SVP26(YHR181W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:TAT2(YOL020W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TUF1(YOR187W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TUM1(YOR251C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VBA1(YMR088C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA1(YDL185W)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS15(YBR097W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS24(YKL041W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS73(YGL104C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBL055C(YBL055C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR027C(YBR027C_d)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YER076C(YER076C_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function Gene:YJL171C(YJL171C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress Gene:YJR142W(YJR142W_p)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL147C(YKL147C_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YLR202C(YLR202C_d)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YPR011C(YPR011C_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ZRT2(YLR130C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:AAT1(YKL106W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ADE1(YAR015W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:APL1(YJR005W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATG14(YBR128C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:BST1(YFL025C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CLU1(YMR012W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX5A(YNL052W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:DIG1(YPL049C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DJP1(YIR004W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DYN3(YMR299C)|FD-Score:4.32|P-value:7.90E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FAR8(YMR029C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FET3(YMR058W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:GET2(YER083C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GOS1(YHL031C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HES1(YOR237W)|FD-Score:7.8|P-value:3.02E-15||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:IRC24(YIR036C_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:LYS14(YDR034C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MCR1(YKL150W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MDM36(YPR083W)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:NCL1(YBL024W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:PGC1(YPL206C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO81(YGR233C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL26B(YGR034W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:SAP1(YER047C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SAP30(YMR263W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAP4(YGL229C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SSF2(YDR312W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:SVP26(YHR181W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:TAT2(YOL020W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TUF1(YOR187W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TUM1(YOR251C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VBA1(YMR088C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA1(YDL185W)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS15(YBR097W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS24(YKL041W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS73(YGL104C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBL055C(YBL055C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR027C(YBR027C_d)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YER076C(YER076C_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function Gene:YJL171C(YJL171C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress Gene:YJR142W(YJR142W_p)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL147C(YKL147C_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YLR202C(YLR202C_d)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YPR011C(YPR011C_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ZRT2(YLR130C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER008C3.721.00E-40.70SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YIL126W3.020.001270.02STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YEL058W3.000.001360.01PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YOL021C2.990.001400.01DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YNL245C2.980.001450.15CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YLR430W2.830.002330.04SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YFL005W2.790.002640.00SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YOR370C2.790.002660.00MRS6Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
YDL145C2.790.002680.02COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YNR011C2.770.002830.02PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YNL126W2.740.003040.09SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YPL093W2.650.004000.05NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YNL062C2.600.004690.00GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YNL310C2.590.004750.07ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YGR113W2.530.005770.04DAM1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR237W7.803.02E-15HES1Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication
YAR015W4.751.02E-6ADE1N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
YMR299C4.327.90E-6DYN3Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
YHR181W4.241.10E-5SVP26Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment
YIR004W4.171.50E-5DJP1Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ
YDR312W4.121.88E-5SSF2Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
YKL106W4.111.95E-5AAT1Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
YPL206C4.052.54E-5PGC1Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs
YHL031C3.973.53E-5GOS1v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28
YGL104C3.953.89E-5VPS73Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family
YNL052W3.953.97E-5COX5ASubunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication
YER076C_p3.934.26E-5YER076C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
YMR158C-A_p3.924.40E-5YMR158C-A_pPutative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene
YMR263W3.904.81E-5SAP30Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
YPR011C_p3.855.92E-5YPR011C_pPutative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_1295
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0764.89E-9SGTC_2170morpholine 200.0 μMChembridge (Fragment library)1211710.112903
0.0756.39E-9SGTC_22917917025 104.3 μMChembridge (Fragment library)29646120.0704225iron homeostasis
0.0641.02E-6SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0526316RPP1 & pyrimidine depletion
0.0631.15E-6SGTC_33269140064 16.4 μMChembridge (Drug-like library)18684340.0740741
0.0571.10E-5SGTC_7791360-0071 233.0 μMChemDiv (Drug-like library)75660.0727273fatty acid desaturase (OLE1)
0.0552.41E-5SGTC_28699037858 32.5 μMChembridge (Drug-like library)271870420.0684932
0.0526.40E-5SGTC_12961068-0102 11.9 μMChemDiv (Drug-like library)7670530.0923077
0.0472.82E-4SGTC_437s-farnesyl-l-cysteine methyl ester 6.7 μMICCB bioactive library64392250.0285714
0.0473.37E-4SGTC_1840st055363 71.0 μMTimTec (Natural product derivative library)6760330.09375
0.0464.61E-4SGTC_30869117350 49.5 μMChembridge (Drug-like library)26792650.0972222
0.0456.10E-4SGTC_1850st056238 17.0 μMTimTec (Natural product derivative library)53931770.126984RPP1 & pyrimidine depletion
0.0439.61E-4SGTC_12570669-0121 16.0 μMChemDiv (Drug-like library)43078550.166667
0.0430.00109SGTC_28257998094 58.4 μMChembridge (Drug-like library)29878150.0617284fatty acid desaturase (OLE1)
0.0420.00131SGTC_6800417-1641 114.0 μMChemDiv (Drug-like library)28359900.0547945RPP1 & pyrimidine depletion
0.0420.00133SGTC_14863970-0793 4.5 μMChemDiv (Drug-like library)28443300.0677966

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8550428-002025 μM0.6578956812414ChemDiv (Drug-like library)226.23072.15914Golgi
SGTC_4271354-005347.3 μM0.4107146813657ChemDiv (Drug-like library)407.892684.19625
SGTC_2304744301691.4 μM0.365385939854Chembridge (Fragment library)260.235592.82415
SGTC_8690564-008044.1 μM0.326843321ChemDiv (Drug-like library)317.34133.93125
SGTC_166k072-0058166.42 μM0.3157895846229ChemDiv (Drug-like library)308.255254.0227DNA intercalators
SGTC_165k038-002364.38 μM0.31034546368543ChemDiv (Drug-like library)383.449276.77804ubiquinone biosynthesis & proteasome
SGTC_5720960-008717.7 μM0.3098596796365ChemDiv (Drug-like library)444.869784.8426
SGTC_1642st00958277.7 μM0.3018876739311TimTec (Natural product derivative library)257.241441.48735RPP1 & pyrimidine depletion
SGTC_432NS 161961.3 μM0.2962964552ICCB bioactive library362.2266394.11728
SGTC_1349flufenamic acid69 μM0.2962963371ChemDiv (Drug-like library)281.229913.95226