1079-1278

4-(5,7-dinitroquinolin-8-yl)morpholine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1298
Screen concentration 263.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2840950
SMILES C1COCCN1C2=C(C=C(C3=C2N=CC=C3)[N+](=O)[O-])[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1cc(c2cccnc2c1N3CCOCC3)[N+](=O)[O-]
Molecular weight 304.2582
ALogP 1.66
H-bond donor count 0
H-bond acceptor count 7
Response signature DNA damage response

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.42
% growth inhibition (Hom. pool) 3.21


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2840950
Download HIP data (tab-delimited text)  (excel)
Gene:CDC15(YAR019C)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:-3.25|P-value:5.82E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DHR2(YKL078W)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.18||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:EMG1(YLR186W)|FD-Score:3.13|P-value:8.67E-4|Clearance:0.21||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GPN2(YOR262W)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.19||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:LCP5(YER127W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.29||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LIP1(YMR298W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.19||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MIF2(YKL089W)|FD-Score:5.01|P-value:2.71E-7|Clearance:0.58||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:RPP1(YHR062C)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SDA1(YGR245C)|FD-Score:3.46|P-value:2.71E-4|Clearance:0.14||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SEC11(YIR022W)|FD-Score:-3.19|P-value:7.02E-4|Clearance:0||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SPC3(YLR066W)|FD-Score:4.43|P-value:4.77E-6|Clearance:0.16||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SRP68(YPL243W)|FD-Score:3.14|P-value:8.48E-4|Clearance:0.01||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TOR2(YKL203C)|FD-Score:4.27|P-value:9.92E-6|Clearance:0.01||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:YLR317W(YLR317W_d)|FD-Score:-4.57|P-value:2.47E-6|Clearance:0||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YOR146W(YOR146W_d)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:CDC15(YAR019C)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:-3.25|P-value:5.82E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DHR2(YKL078W)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.18||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:EMG1(YLR186W)|FD-Score:3.13|P-value:8.67E-4|Clearance:0.21||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GPN2(YOR262W)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.19||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:LCP5(YER127W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.29||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LIP1(YMR298W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.19||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MIF2(YKL089W)|FD-Score:5.01|P-value:2.71E-7|Clearance:0.58||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:RPP1(YHR062C)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SDA1(YGR245C)|FD-Score:3.46|P-value:2.71E-4|Clearance:0.14||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:SEC11(YIR022W)|FD-Score:-3.19|P-value:7.02E-4|Clearance:0||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SPC3(YLR066W)|FD-Score:4.43|P-value:4.77E-6|Clearance:0.16||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SRP68(YPL243W)|FD-Score:3.14|P-value:8.48E-4|Clearance:0.01||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TOR2(YKL203C)|FD-Score:4.27|P-value:9.92E-6|Clearance:0.01||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:YLR317W(YLR317W_d)|FD-Score:-4.57|P-value:2.47E-6|Clearance:0||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YOR146W(YOR146W_d)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2840950
Download HOP data (tab-delimited text)  (excel)
Gene:ANB1(YJR047C)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ARA1(YBR149W)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARE1(YCR048W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:ARP8(YOR141C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:BDF2(YDL070W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BUD25(YER014C-A)|FD-Score:-4.5|P-value:3.47E-6||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CCP1(YKR066C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CEM1(YER061C)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CTR1(YPR124W)|FD-Score:-6.27|P-value:1.84E-10||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DBR1(YKL149C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:FKH1(YIL131C)|FD-Score:4|P-value:3.10E-5||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FLC1(YPL221W)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP48(YGR052W_p)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GEM1(YAL048C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GEP5(YLR091W)|FD-Score:4.88|P-value:5.23E-7||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HAL1(YPR005C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HSL7(YBR133C)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HUG1(YML058W-A)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IWR1(YDL115C)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAC1(YMR021C)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEP1(YGR121C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET31(YPL038W)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MIG2(YGL209W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MRPL22(YNL177C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSE1(YOL033W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NIF3(YGL221C)|FD-Score:-4.39|P-value:5.63E-6||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NUP60(YAR002W)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:OCT1(YKL134C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PCP1(YGR101W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PFD1(YJL179W)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PRO2(YOR323C)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD18(YCR066W)|FD-Score:4.8|P-value:7.89E-7||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:6.13|P-value:4.33E-10||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD57(YDR004W)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RHB1(YCR027C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL23A(YBL087C)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS12(YOR369C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RRF1(YHR038W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RTT101(YJL047C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAE3(YHR079C-A)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SPO74(YGL170C)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SUR4(YLR372W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUV3(YPL029W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWS2(YNL081C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA3(YHR009C_p)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:THP1(YOL072W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TRM732(YMR259C_p)|FD-Score:4.85|P-value:6.05E-7||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UME6(YDR207C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VMS1(YDR049W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS4(YPR173C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBR099C(YBR099C_d)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YDR282C(YDR282C_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YDR379C-A(YDR379C-A)|FD-Score:-4.07|P-value:2.35E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YER135C(YER135C_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function Gene:YIR020W-A(YIR020W-A_d)|FD-Score:5.83|P-value:2.73E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL215C(YJL215C_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL222C(YKL222C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR171W(YLR171W_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:-4.32|P-value:7.65E-6||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YOL114C(YOL114C_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR050C(YOR050C_d)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YPL014W(YPL014W_p)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPR123C(YPR123C_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT7(YML001W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:ANB1(YJR047C)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ARA1(YBR149W)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARE1(YCR048W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:ARP8(YOR141C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:BDF2(YDL070W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BUD25(YER014C-A)|FD-Score:-4.5|P-value:3.47E-6||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CCP1(YKR066C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CEM1(YER061C)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CTR1(YPR124W)|FD-Score:-6.27|P-value:1.84E-10||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DBR1(YKL149C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:FKH1(YIL131C)|FD-Score:4|P-value:3.10E-5||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FLC1(YPL221W)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP48(YGR052W_p)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GEM1(YAL048C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GEP5(YLR091W)|FD-Score:4.88|P-value:5.23E-7||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:HAL1(YPR005C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HSL7(YBR133C)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HUG1(YML058W-A)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IWR1(YDL115C)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAC1(YMR021C)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEP1(YGR121C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET31(YPL038W)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MIG2(YGL209W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MRPL22(YNL177C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSE1(YOL033W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NIF3(YGL221C)|FD-Score:-4.39|P-value:5.63E-6||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NUP60(YAR002W)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:OCT1(YKL134C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PCP1(YGR101W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PFD1(YJL179W)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PRO2(YOR323C)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD18(YCR066W)|FD-Score:4.8|P-value:7.89E-7||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:6.13|P-value:4.33E-10||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD54(YGL163C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD57(YDR004W)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RHB1(YCR027C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL23A(YBL087C)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS12(YOR369C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RRF1(YHR038W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RTT101(YJL047C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAE3(YHR079C-A)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SPO74(YGL170C)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SUR4(YLR372W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUV3(YPL029W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWS2(YNL081C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TDA3(YHR009C_p)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:THP1(YOL072W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TRM732(YMR259C_p)|FD-Score:4.85|P-value:6.05E-7||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UME6(YDR207C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VMS1(YDR049W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS4(YPR173C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBR099C(YBR099C_d)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YDR282C(YDR282C_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YDR379C-A(YDR379C-A)|FD-Score:-4.07|P-value:2.35E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YER135C(YER135C_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function Gene:YIR020W-A(YIR020W-A_d)|FD-Score:5.83|P-value:2.73E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL215C(YJL215C_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL222C(YKL222C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR171W(YLR171W_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:-4.32|P-value:7.65E-6||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YOL114C(YOL114C_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR050C(YOR050C_d)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YPL014W(YPL014W_p)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPR123C(YPR123C_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT7(YML001W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL089W5.012.71E-70.58MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YLR066W4.434.77E-60.16SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YKL203C4.279.92E-60.01TOR2PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
YER127W4.251.06E-50.29LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YOR262W3.963.72E-50.18GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YOR146W_d3.787.96E-50.13YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YMR298W3.651.33E-40.19LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YGR245C3.462.71E-40.14SDA1Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
YKL078W3.324.46E-40.18DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YPL243W3.148.48E-40.01SRP68Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YLR186W3.138.67E-40.21EMG1Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
YKL018W2.920.001720.05SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YOL040C2.870.002040.04RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YGL130W2.830.002290.06CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YDL143W2.780.002730.09CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR032W6.134.33E-10RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YIR020W-A_d5.832.73E-9YIR020W-A_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YDR004W5.181.13E-7RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YJR047C5.171.19E-7ANB1Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication
YLR091W4.885.23E-7GEP5Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
YMR259C_p4.856.05E-7TRM732_pInteracts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene
YCR066W4.807.89E-7RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YDL115C4.798.32E-7IWR1RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
YHR009C_p4.552.69E-6TDA3_pPutative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
YLR171W_d4.241.12E-5YLR171W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL163C4.231.15E-5RAD54DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
YGR101W4.151.65E-5PCP1Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
YLR372W4.082.29E-5SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YPL221W4.013.04E-5FLC1Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance
YIL131C4.003.10E-5FKH1Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1298
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1223.59E-21SGTC_2677mitomycin C 100.0 μMMiscellaneous57460.045977DNA damage response
0.1226.45E-21SGTC_244nsc-207895 1.3 μMMiscellaneous426400.285714DNA damage response
0.0992.12E-14SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.0704225
0.0953.46E-13SGTC_1936st074407 74.3 μMTimTec (Natural product derivative library)8098130.15942DNA damage response
0.0937.68E-13SGTC_251melphalan 456.5 μMMiscellaneous4606120.057971DNA damage response
0.0856.15E-11SGTC_248streptozotocin 5.2 mMMiscellaneous53000.027027DNA damage response
0.0857.54E-11SGTC_32549136651 49.5 μMChembridge (Drug-like library)164540300.0634921DNA damage response
0.0831.42E-10SGTC_2674-Nitroquinoline-1-oxide (4-NQO) 44.4 nMMiscellaneous59550.264151DNA damage response
0.0831.76E-10SGTC_4291112-0049 25.5 μMChemDiv (Drug-like library)28361700.0810811DNA damage response
0.0766.17E-9SGTC_12460422-0085 105.0 μMChemDiv (Drug-like library)2642950.105263
0.0723.75E-8SGTC_2759disulfiram 3.4 μMMiscellaneous3117NA
0.0706.43E-8SGTC_5504123-0385 25.7 μMChemDiv (Drug-like library)57311230.175676TSC3-RPN4
0.0691.29E-7SGTC_1082camptothecin 424.9 nMTimTec (Natural product library)25380.117647DNA damage response
0.0682.15E-7SGTC_915methyl methanesulfonate (MMS) 110.0 mMMiscellaneous41560.0204082DNA damage response
0.0663.94E-7SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0571429mitochondrial stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1832st05411355 μM0.4590161251267TimTec (Natural product derivative library)364.39783.37116
SGTC_1640st00907866.4 μM0.396825690618TimTec (Natural product derivative library)301.340362.91115
SGTC_2784756767372.73 μM0.3928573471247Chembridge (Drug-like library)343.42011.29616iron homeostasis
SGTC_524r073-001027.8 μM0.3684212772106ChemDiv (Drug-like library)284.655861.80807
SGTC_20104012422159 μM0.307692952393Chembridge (Fragment library)212.676021.59713
SGTC_7930488-0545106 μM0.301887292548ChemDiv (Drug-like library)237.212042.24105
SGTC_2816798270658.44 μM0.2985072981853Chembridge (Drug-like library)326.392883.50115azole & statin
SGTC_1824st05372368.6 μM0.298246675225TimTec (Natural product derivative library)291.347083.39704
SGTC_244nsc-2078951.3 μM0.28571442640Miscellaneous279.25202-1.27707DNA damage response
SGTC_1360097-0015404.48 μM0.2830194861337ChemDiv (Drug-like library)494.448642.62701160S ribosome export