0199-0087

[5-chloro-2-[(4-chlorophenyl)methylideneamino]phenyl]-phenylmethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_13
Screen concentration 65.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4589825
SMILES C1=CC=C(C=C1)C(=O)C2=C(C=CC(=C2)Cl)N=CC3=CC=C(C=C3)Cl
Standardized SMILES Clc1ccc(C=Nc2ccc(Cl)cc2C(=O)c3ccccc3)cc1
Molecular weight 354.2293
ALogP 6.16
H-bond donor count 0
H-bond acceptor count 2
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 34.85
% growth inhibition (Hom. pool) 22.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4589825
Download HIP data (tab-delimited text)  (excel)
Gene:CET1(YPL228W)|FD-Score:5.35|P-value:4.42E-8|Clearance:0.73||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:DFR1(YOR236W)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.05||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FAS1(YKL182W)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HEM15(YOR176W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HRR25(YPL204W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.04||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:IRR1(YIL026C)|FD-Score:3.27|P-value:5.41E-4|Clearance:0.04||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NSE4(YDL105W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.05||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RPL32(YBL092W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SAD1(YFR005C)|FD-Score:3.5|P-value:2.32E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SPP41(YDR464W)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.05||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:YBR124W(YBR124W_d)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.12||SGD DESC:Putative protein of unknown function Gene:YCG1(YDR325W)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.42||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YLR458W(YLR458W_d)|FD-Score:4.69|P-value:1.33E-6|Clearance:0.73||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YRB1(YDR002W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.05||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:CET1(YPL228W)|FD-Score:5.35|P-value:4.42E-8|Clearance:0.73||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:DFR1(YOR236W)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.05||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FAS1(YKL182W)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HEM15(YOR176W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HRR25(YPL204W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.04||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:IRR1(YIL026C)|FD-Score:3.27|P-value:5.41E-4|Clearance:0.04||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NSE4(YDL105W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.05||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RPL32(YBL092W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SAD1(YFR005C)|FD-Score:3.5|P-value:2.32E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SPP41(YDR464W)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.05||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:YBR124W(YBR124W_d)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.12||SGD DESC:Putative protein of unknown function Gene:YCG1(YDR325W)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.42||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YLR458W(YLR458W_d)|FD-Score:4.69|P-value:1.33E-6|Clearance:0.73||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YRB1(YDR002W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.05||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4589825
Download HOP data (tab-delimited text)  (excel)
Gene:AGA2(YGL032C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:APN1(YKL114C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG3(YJL088W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO7(YPR060C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:CTF8(YHR191C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CTT1(YGR088W)|FD-Score:-6.45|P-value:5.53E-11||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DAL81(YIR023W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ELA1(YNL230C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERG2(YMR202W)|FD-Score:8.65|P-value:2.56E-18||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:HIS6(YIL020C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IES2(YNL215W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:MTC4(YBR255W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MUP1(YGR055W)|FD-Score:7.64|P-value:1.12E-14||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NIP100(YPL174C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NPL3(YDR432W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NRP1(YDL167C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:PAM1(YDR251W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:QCR9(YGR183C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RGL1(YPL066W)|FD-Score:6.93|P-value:2.06E-12||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL37B(YDR500C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:TUB3(YML124C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:4.91|P-value:4.55E-7||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YBR296C-A(YBR296C-A_p)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YEL023C(YEL023C_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YMR099C(YMR099C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YOL118C(YOL118C_d)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:6.15|P-value:3.79E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR325W(YOR325W_d)|FD-Score:5.4|P-value:3.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:AGA2(YGL032C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:APN1(YKL114C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG3(YJL088W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO7(YPR060C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:CTF8(YHR191C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CTT1(YGR088W)|FD-Score:-6.45|P-value:5.53E-11||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DAL81(YIR023W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ELA1(YNL230C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERG2(YMR202W)|FD-Score:8.65|P-value:2.56E-18||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:HIS6(YIL020C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IES2(YNL215W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:MTC4(YBR255W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MUP1(YGR055W)|FD-Score:7.64|P-value:1.12E-14||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NIP100(YPL174C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NPL3(YDR432W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NRP1(YDL167C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:PAM1(YDR251W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:QCR9(YGR183C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RGL1(YPL066W)|FD-Score:6.93|P-value:2.06E-12||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL37B(YDR500C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:TUB3(YML124C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:4.91|P-value:4.55E-7||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YBR296C-A(YBR296C-A_p)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YEL023C(YEL023C_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YMR099C(YMR099C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YOL118C(YOL118C_d)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:6.15|P-value:3.79E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR325W(YOR325W_d)|FD-Score:5.4|P-value:3.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL228W5.354.42E-80.73CET1Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YLR458W_d4.691.33E-60.73YLR458W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis
YDR325W3.963.70E-50.42YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YPL204W3.541.97E-40.04HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YFR005C3.502.32E-40.00SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YDL105W3.502.33E-40.05NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YOR236W3.452.83E-40.05DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YBL092W3.403.38E-40.08RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YDR464W3.324.55E-40.05SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YIL026C3.275.41E-40.04IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YOR176W3.226.31E-40.00HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YBR124W_d3.226.38E-40.12YBR124W_dPutative protein of unknown function
YDR002W3.109.69E-40.05YRB1Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
YGL044C3.050.001140.03RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YKR083C3.020.001260.01DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W8.652.56E-18ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YGR055W7.641.12E-14MUP1High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
YPL066W6.932.06E-12RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YOR072W_d6.153.79E-10YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YOR325W_d5.403.26E-8YOR325W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1
YNL229C4.914.55E-7URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YDR251W4.318.19E-6PAM1Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype
YGL032C4.092.18E-5AGA2Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
YBR296C-A_p4.062.41E-5YBR296C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YKL114C3.924.34E-5APN1Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine
YMR099C3.661.27E-4YMR099CGlucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
YJL088W3.621.46E-4ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YGR183C3.561.87E-4QCR9Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
YIR023W3.522.19E-4DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YOL118C_d3.482.48E-4YOL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_13
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2201.57E-65SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0793651ERG2
0.2126.55E-61SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.114754ERG2
0.2054.09E-57SGTC_140354-0009 295.0 μMChemDiv (Drug-like library)17135070.44
0.1842.59E-46SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0576923ERG2
0.1805.01E-44SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0508475ERG2
0.1784.59E-43SGTC_9741326-0144 138.0 μMChemDiv (Drug-like library)54118560.0847458ERG2
0.1694.94E-39SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.109589ERG2
0.1632.08E-36SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.12987ERG2
0.1483.17E-30SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.1ERG2
0.1461.15E-29SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.220339
0.1412.02E-27SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.147059ERG2
0.1381.41E-26SGTC_2734clemastine 41.6 μMMiscellaneous269870.164179ERG2
0.1381.56E-26SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.126761ERG2
0.1373.49E-26SGTC_2523spermine 47.7 μMMicrosource (Natural product library)1103NAcalcium & mitochondrial duress
0.1366.15E-26SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.242424ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_80354-000170 μM0.8048784993152ChemDiv (Drug-like library)354.229326.15902
SGTC_8700573-221357.6 μM0.7142863916321ChemDiv (Drug-like library)453.91636.78104ERG2
SGTC_310508-197457.24 μM0.5882356810525ChemDiv (Drug-like library)414.679724.6213
SGTC_12700831-117017.3 μM0.4901966764716ChemDiv (Drug-like library)380.234664.25113
SGTC_7560508-199928.3 μM0.4464296753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_140354-0009295 μM0.441713507ChemDiv (Drug-like library)338.626824.51612
SGTC_1608st00147340.5 μM0.4318187292267TimTec (Natural product derivative library)242.700244.36601
SGTC_7051124-0212310 μM0.429554485ChemDiv (Drug-like library)293.1484.01212
SGTC_10154130-452582 μM0.41363237ChemDiv (Drug-like library)392.834983.8032360S ribosome export
SGTC_5810958-0271211 μM0.389834591145ChemDiv (Drug-like library)379.836226.25314