0136-0219

2-(4-chlorophenoxy)-N-(2-chlorophenyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_130
Screen concentration 337.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 234244
SMILES C1=CC=C(C(=C1)NC(=O)COC2=CC=C(C=C2)Cl)Cl
Standardized SMILES Clc1ccc(OCC(=O)Nc2ccccc2Cl)cc1
Molecular weight 296.1486
ALogP 3.78
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.3
% growth inhibition (Hom. pool) 11.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 234244
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:-3.2|P-value:6.92E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT3(YJL014W)|FD-Score:3.33|P-value:4.38E-4|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.04||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC15(YAR019C)|FD-Score:-3.94|P-value:4.12E-5|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CWC25(YNL245C)|FD-Score:4.43|P-value:4.63E-6|Clearance:0.17||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DAD2(YKR083C)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.08||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DSL1(YNL258C)|FD-Score:3.2|P-value:6.93E-4|Clearance:0.04||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:ERG29(YMR134W_p)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GCD2(YGR083C)|FD-Score:5.66|P-value:7.76E-9|Clearance:0.66||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ILV5(YLR355C)|FD-Score:-3.1|P-value:9.65E-4|Clearance:0||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:LIP1(YMR298W)|FD-Score:4.89|P-value:5.09E-7|Clearance:0.04||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MCD1(YDL003W)|FD-Score:-3.25|P-value:5.83E-4|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MPE1(YKL059C)|FD-Score:4.45|P-value:4.31E-6|Clearance:0.02||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NUF2(YOL069W)|FD-Score:3.22|P-value:6.44E-4|Clearance:0.02||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:ORC6(YHR118C)|FD-Score:3.76|P-value:8.61E-5|Clearance:0.12||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PDS5(YMR076C)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.1||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE1(YER012W)|FD-Score:4.85|P-value:6.16E-7|Clearance:0.09||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRP31(YGR091W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.27||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP45(YDR280W)|FD-Score:5.54|P-value:1.47E-8|Clearance:0.66||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC13(YLR208W)|FD-Score:4.16|P-value:1.56E-5|Clearance:0.34||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YBR124W(YBR124W_d)|FD-Score:4.76|P-value:9.85E-7|Clearance:0.31||SGD DESC:Putative protein of unknown function Gene:YOR146W(YOR146W_d)|FD-Score:3.83|P-value:6.47E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ARP9(YMR033W)|FD-Score:-3.2|P-value:6.92E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT3(YJL014W)|FD-Score:3.33|P-value:4.38E-4|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.04||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC15(YAR019C)|FD-Score:-3.94|P-value:4.12E-5|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CWC25(YNL245C)|FD-Score:4.43|P-value:4.63E-6|Clearance:0.17||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DAD2(YKR083C)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.08||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DSL1(YNL258C)|FD-Score:3.2|P-value:6.93E-4|Clearance:0.04||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:ERG29(YMR134W_p)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GCD2(YGR083C)|FD-Score:5.66|P-value:7.76E-9|Clearance:0.66||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ILV5(YLR355C)|FD-Score:-3.1|P-value:9.65E-4|Clearance:0||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:LIP1(YMR298W)|FD-Score:4.89|P-value:5.09E-7|Clearance:0.04||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MCD1(YDL003W)|FD-Score:-3.25|P-value:5.83E-4|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MPE1(YKL059C)|FD-Score:4.45|P-value:4.31E-6|Clearance:0.02||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NUF2(YOL069W)|FD-Score:3.22|P-value:6.44E-4|Clearance:0.02||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:ORC6(YHR118C)|FD-Score:3.76|P-value:8.61E-5|Clearance:0.12||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PDS5(YMR076C)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.1||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE1(YER012W)|FD-Score:4.85|P-value:6.16E-7|Clearance:0.09||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRP31(YGR091W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.27||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP45(YDR280W)|FD-Score:5.54|P-value:1.47E-8|Clearance:0.66||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC13(YLR208W)|FD-Score:4.16|P-value:1.56E-5|Clearance:0.34||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YBR124W(YBR124W_d)|FD-Score:4.76|P-value:9.85E-7|Clearance:0.31||SGD DESC:Putative protein of unknown function Gene:YOR146W(YOR146W_d)|FD-Score:3.83|P-value:6.47E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 234244
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADH2(YMR303C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AIM32(YML050W)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APL3(YBL037W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:AQR1(YNL065W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ARO2(YGL148W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARR2(YPR200C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATG23(YLR431C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:CAT2(YML042W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COG5(YNL051W)|FD-Score:-3.93|P-value:4.26E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR2(YHR057C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRP1(YHR146W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CYS3(YAL012W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DCW1(YKL046C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DIG1(YPL049C)|FD-Score:5.01|P-value:2.78E-7||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DST1(YGL043W)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ERG3(YLR056W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:EXO1(YOR033C)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-8.04|P-value:4.44E-16||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GEA1(YJR031C)|FD-Score:5.32|P-value:5.11E-8||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GLN3(YER040W)|FD-Score:3.84|P-value:6.14E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HHF1(YBR009C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:ICL2(YPR006C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:ICT1(YLR099C)|FD-Score:-5.49|P-value:2.04E-8||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IES5(YER092W)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:INP54(YOL065C)|FD-Score:5.92|P-value:1.65E-9||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:IRC24(YIR036C_p)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:LSM12(YHR121W)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LST7(YGR057C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAL13(YGR288W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAM1(YER106W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MDM31(YHR194W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MEI5(YPL121C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:MMP1(YLL061W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MNN2(YBR015C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOT2(YER068W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPL15(YLR312W-A)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MTM1(YGR257C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MTQ2(YDR140W)|FD-Score:10.2|P-value:1.28E-24||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NIT1(YIL164C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NTC20(YBR188C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OCA5(YHL029C_p)|FD-Score:-5.3|P-value:5.92E-8||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OYE2(YHR179W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PET122(YER153C)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PEX18(YHR160C)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX30(YLR324W)|FD-Score:-3.94|P-value:4.01E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:POC4(YPL144W)|FD-Score:5.27|P-value:6.75E-8||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPS1(YBR276C)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD1(YPL022W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RED1(YLR263W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RFU1(YLR073C)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL7B(YPL198W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS22B(YLR367W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:SAP30(YMR263W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAS4(YDR181C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SCS22(YBL091C-A)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:6.94|P-value:1.95E-12||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKY1(YMR216C)|FD-Score:5.35|P-value:4.35E-8||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPG1(YGR236C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:STB6(YKL072W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:STO1(YMR125W)|FD-Score:3.75|P-value:8.69E-5||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWM2(YNR004W_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:SWT21(YNL187W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TDA9(YML081W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:THI12(YNL332W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:THI4(YGR144W)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TRK2(YKR050W)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UFO1(YML088W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VBA2(YBR293W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VEL1(YGL258W)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS28(YPL065W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YBR013C(YBR013C_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCK3(YER123W)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YEA6(YEL006W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YEL1(YBL060W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YGR066C(YGR066C_p)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR210C(YGR210C_p)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR003C(YHR003C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YHR080C(YHR080C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIH1(YCR059C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIR014W(YIR014W_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YIR021W-A(YIR021W-A_p)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIR044C(YIR044C)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YLL044W(YLL044W_d)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR031W(YLR031W_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function Gene:YLR283W(YLR283W_p)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR210W(YMR210W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YMR252C(YMR252C_p)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YNL190W(YNL190W_p)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YOR238W(YOR238W_p)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YOR300W(YOR300W_d)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOR365C(YOR365C_p)|FD-Score:6.23|P-value:2.34E-10||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPL071C(YPL071C_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL260W(YPL260W_p)|FD-Score:-4.42|P-value:4.98E-6||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR197C(YPR197C_d)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACM1(YPL267W)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADH2(YMR303C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AIM32(YML050W)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APL3(YBL037W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:AQR1(YNL065W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ARO2(YGL148W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARR2(YPR200C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATG23(YLR431C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:CAT2(YML042W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COG5(YNL051W)|FD-Score:-3.93|P-value:4.26E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR2(YHR057C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRP1(YHR146W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CYS3(YAL012W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DCW1(YKL046C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DIG1(YPL049C)|FD-Score:5.01|P-value:2.78E-7||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DST1(YGL043W)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ERG3(YLR056W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:EXO1(YOR033C)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-8.04|P-value:4.44E-16||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GEA1(YJR031C)|FD-Score:5.32|P-value:5.11E-8||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GLN3(YER040W)|FD-Score:3.84|P-value:6.14E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HHF1(YBR009C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:ICL2(YPR006C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:ICT1(YLR099C)|FD-Score:-5.49|P-value:2.04E-8||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IES5(YER092W)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:INP54(YOL065C)|FD-Score:5.92|P-value:1.65E-9||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:IRC24(YIR036C_p)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:LSM12(YHR121W)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LST7(YGR057C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAL13(YGR288W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAM1(YER106W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MDM31(YHR194W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MEI5(YPL121C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:MMP1(YLL061W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MNN2(YBR015C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOT2(YER068W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPL15(YLR312W-A)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MTM1(YGR257C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MTQ2(YDR140W)|FD-Score:10.2|P-value:1.28E-24||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NIT1(YIL164C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NTC20(YBR188C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OCA5(YHL029C_p)|FD-Score:-5.3|P-value:5.92E-8||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OYE2(YHR179W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PET122(YER153C)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PEX18(YHR160C)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX30(YLR324W)|FD-Score:-3.94|P-value:4.01E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:POC4(YPL144W)|FD-Score:5.27|P-value:6.75E-8||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPS1(YBR276C)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD1(YPL022W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RED1(YLR263W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RFU1(YLR073C)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL7B(YPL198W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS22B(YLR367W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:SAP30(YMR263W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAS4(YDR181C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SCS22(YBL091C-A)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:6.94|P-value:1.95E-12||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKY1(YMR216C)|FD-Score:5.35|P-value:4.35E-8||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPG1(YGR236C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:STB6(YKL072W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:STO1(YMR125W)|FD-Score:3.75|P-value:8.69E-5||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWM2(YNR004W_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:SWT21(YNL187W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TDA9(YML081W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:THI12(YNL332W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:THI4(YGR144W)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TRK2(YKR050W)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UFO1(YML088W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VBA2(YBR293W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VEL1(YGL258W)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS28(YPL065W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YBR013C(YBR013C_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCK3(YER123W)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YEA6(YEL006W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YEL1(YBL060W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YGR066C(YGR066C_p)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR210C(YGR210C_p)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR003C(YHR003C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YHR080C(YHR080C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIH1(YCR059C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIR014W(YIR014W_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YIR021W-A(YIR021W-A_p)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIR044C(YIR044C)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YLL044W(YLL044W_d)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR031W(YLR031W_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function Gene:YLR283W(YLR283W_p)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR210W(YMR210W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YMR252C(YMR252C_p)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YNL190W(YNL190W_p)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YOR238W(YOR238W_p)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YOR300W(YOR300W_d)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOR365C(YOR365C_p)|FD-Score:6.23|P-value:2.34E-10||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPL071C(YPL071C_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL260W(YPL260W_p)|FD-Score:-4.42|P-value:4.98E-6||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR197C(YPR197C_d)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR083C5.667.76E-90.66GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YDR280W5.541.47E-80.66RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YMR298W4.895.09E-70.04LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YER012W4.856.16E-70.09PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YBR124W_d4.769.85E-70.31YBR124W_dPutative protein of unknown function
YKL059C4.454.31E-60.02MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YNL245C4.434.63E-60.17CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YMR076C4.261.01E-50.10PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YLR208W4.161.56E-50.34SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YOR146W_d3.836.47E-50.07YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YHR118C3.768.61E-50.12ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YDL143W3.641.37E-40.04CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR091W3.601.60E-40.27PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YJL014W3.334.38E-40.11CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YOL069W3.226.44E-40.02NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR140W10.201.28E-24MTQ2S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
YPR198W6.941.95E-12SGE1Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
YOR365C_p6.232.34E-10YOR365C_pPutative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication
YOL065C5.921.65E-9INP54Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
YOR238W_p5.531.62E-8YOR238W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YMR216C5.354.35E-8SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YJR031C5.325.11E-8GEA1Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
YPL144W5.276.75E-8POC4Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
YHR121W5.121.49E-7LSM12Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
YPL049C5.012.78E-7DIG1MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p
YOR300W_d4.904.75E-7YOR300W_dDubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
YLL045C4.681.44E-6RPL8BRibosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
YPL267W4.473.88E-6ACM1Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein
YPR197C_d4.434.71E-6YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR252C_p4.337.59E-6YMR252C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene

GO enrichment analysis for SGTC_130
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1088.23E-17SGTC_1330724-0033 378.9 μMChemDiv (Drug-like library)2803090.101695
0.0809.85E-10SGTC_22376667747 47.2 μMChembridge (Fragment library)7299400.0819672
0.0791.14E-9SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.155172
0.0681.47E-7SGTC_1823448-4371 550.5 μMChemDiv (Drug-like library)34914030.2
0.0672.31E-7SGTC_32539135987 49.5 μMChembridge (Drug-like library)41824300.14492860S ribosome export
0.0647.51E-7SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.0625azole & statin
0.0612.72E-6SGTC_7323970-1727 52.7 μMChemDiv (Drug-like library)12756990.146667
0.0603.62E-6SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.0684932RPP1 & pyrimidine depletion
0.0594.87E-6SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.157895
0.0596.51E-6SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.125
0.0588.75E-6SGTC_12971068-0111 14.1 μMChemDiv (Drug-like library)7670570.288462
0.0589.55E-6SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.169492
0.0561.47E-5SGTC_750159-0059 199.0 μMChemDiv (Drug-like library)53772700.147541fatty acid desaturase (OLE1)
0.0561.71E-5SGTC_1466k213-0079 57.5 μMChemDiv (Drug-like library)36579810.21875
0.0561.75E-5SGTC_1769st044406 64.4 μMTimTec (Natural product derivative library)6709030.0882353

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_74000s-0487160.28 μM0.619048677548ChemDiv (Drug-like library)277.702982.87223
SGTC_7950685-026578 μM0.571429302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid
SGTC_2826799254119.48 μM0.558142985066Chembridge (Drug-like library)297.6845063.52514
SGTC_6530203-001655.2 μM0.545455295302ChemDiv (Drug-like library)289.756743.44212
SGTC_2822799471871.43 μM0.5094341018011Chembridge (Drug-like library)373.876463.58214
SGTC_22436634756200 μM0.488372885676Chembridge (Fragment library)234.079262.01512
SGTC_22446635614200 μM0.488372836499Chembridge (Fragment library)234.079262.01512
SGTC_13742889-5571141 μM0.4313732853281ChemDiv (Drug-like library)269.724041.89613RSC complex & mRNA processing
SGTC_22456637752200 μM0.428571681890Chembridge (Fragment library)199.63421.35112
SGTC_2895905444758.44 μM0.4285712697450Chembridge (Drug-like library)343.850483.16113