1218-2183

N-[4-[(5-nitro-6-oxocyclohexa-2,4-dien-1-ylidene)methylamino]phenyl]acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1312
Screen concentration 165.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6744073
SMILES CC(=O)NC1=CC=C(C=C1)NC=C2C=CC=C(C2=O)[N+](=O)[O-]
Standardized SMILES CC(=O)Nc1ccc(cc1)N=Cc2cccc(c2O)[N+](=O)[O-]
Molecular weight 299.2814
ALogP 0.96
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.32
% growth inhibition (Hom. pool) 2.05


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6744073
Download HIP data (tab-delimited text)  (excel)
Gene:BET3(YKR068C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CDC16(YKL022C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.08||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:ERG12(YMR208W)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG9(YHR190W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:MCM4(YPR019W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.23||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:MET4(YNL103W)|FD-Score:3.18|P-value:7.33E-4|Clearance:0.09||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:NIC96(YFR002W)|FD-Score:-3.3|P-value:4.92E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PBN1(YCL052C)|FD-Score:-3.34|P-value:4.19E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RIO2(YNL207W)|FD-Score:-3.34|P-value:4.13E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:SPC98(YNL126W)|FD-Score:-3.42|P-value:3.17E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF9(YMR236W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.06||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TFA2(YKR062W)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.44||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UTP25(YIL091C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.04||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:BET3(YKR068C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CDC16(YKL022C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.08||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:ERG12(YMR208W)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG9(YHR190W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:MCM4(YPR019W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.23||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:MET4(YNL103W)|FD-Score:3.18|P-value:7.33E-4|Clearance:0.09||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:NIC96(YFR002W)|FD-Score:-3.3|P-value:4.92E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PBN1(YCL052C)|FD-Score:-3.34|P-value:4.19E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RIO2(YNL207W)|FD-Score:-3.34|P-value:4.13E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:SPC98(YNL126W)|FD-Score:-3.42|P-value:3.17E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF9(YMR236W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.06||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TFA2(YKR062W)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.44||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UTP25(YIL091C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.04||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6744073
Download HOP data (tab-delimited text)  (excel)
Gene:ART10(YLR392C)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:ASH1(YKL185W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:COA2(YPL189C-A)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COR1(YBL045C)|FD-Score:-4.4|P-value:5.49E-6||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:ELM1(YKL048C)|FD-Score:-4.42|P-value:4.93E-6||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FMP23(YBR047W_p)|FD-Score:4.4|P-value:5.45E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV10(YIL097W)|FD-Score:-4.34|P-value:7.12E-6||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GIC2(YDR309C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region Gene:GIS1(YDR096W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:MNN10(YDR245W)|FD-Score:3.19|P-value:7.24E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MSL1(YIR009W)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NPY1(YGL067W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member Gene:PHO88(YBR106W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RCO1(YMR075W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RNH203(YLR154C)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPL21A(YBR191W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SNF4(YGL115W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TGL2(YDR058C)|FD-Score:6|P-value:9.77E-10||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TPN1(YGL186C)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VPS4(YPR173C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBP2(YGL060W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR051W(YBR051W_d)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YDL068W(YDL068W_d)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.31|P-value:8.34E-6||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YIL089W(YIL089W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YJR154W(YJR154W_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL106C-A(YKL106C-A_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi Gene:YLR252W(YLR252W_d)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YMR206W(YMR206W_p)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YPR011C(YPR011C_p)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ART10(YLR392C)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:ASH1(YKL185W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:COA2(YPL189C-A)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COR1(YBL045C)|FD-Score:-4.4|P-value:5.49E-6||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:ELM1(YKL048C)|FD-Score:-4.42|P-value:4.93E-6||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FMP23(YBR047W_p)|FD-Score:4.4|P-value:5.45E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV10(YIL097W)|FD-Score:-4.34|P-value:7.12E-6||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GIC2(YDR309C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region Gene:GIS1(YDR096W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:MNN10(YDR245W)|FD-Score:3.19|P-value:7.24E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MSL1(YIR009W)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NPY1(YGL067W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member Gene:PHO88(YBR106W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RCO1(YMR075W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RNH203(YLR154C)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPL21A(YBR191W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SNF4(YGL115W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TGL2(YDR058C)|FD-Score:6|P-value:9.77E-10||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TPN1(YGL186C)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VPS4(YPR173C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBP2(YGL060W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR051W(YBR051W_d)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YDL068W(YDL068W_d)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.31|P-value:8.34E-6||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YIL089W(YIL089W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YJR154W(YJR154W_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL106C-A(YKL106C-A_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi Gene:YLR252W(YLR252W_d)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YMR206W(YMR206W_p)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YPR011C(YPR011C_p)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKR062W3.983.47E-50.44TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YIL091C3.542.01E-40.04UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YPR019W3.502.36E-40.23MCM4Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
YKL022C3.275.44E-40.08CDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
YHR190W3.187.26E-40.00ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YNL103W3.187.33E-40.09MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YMR236W3.099.95E-40.06TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YBL014C3.030.001220.11RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YJR068W2.920.001720.05RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YBL040C2.880.002000.06ERD2HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
YKL089W2.820.002420.10MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YLR298C2.720.003250.01YHC1Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
YOR181W2.710.003340.06LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YLR275W2.660.003950.03SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YHR070W2.630.004270.01TRM5tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR058C6.009.77E-10TGL2Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
YPR011C_p5.901.79E-9YPR011C_pPutative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKL106C-A_p4.641.72E-6YKL106C-A_pPutative protein of unknown function; identified by homology to uncharacterized proteins in other fungi
YBR051W_d4.454.38E-6YBR051W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase
YBR047W_p4.405.45E-6FMP23_pPutative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR154C4.013.03E-5RNH203Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome
YMR075W3.973.64E-5RCO1Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p
YGL115W3.944.01E-5SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YDR096W3.904.78E-5GIS1Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
YGL186C3.826.66E-5TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YIR009W3.807.26E-5MSL1U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
YBR191W3.807.35E-5RPL21ARibosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
YMR206W_p3.797.65E-5YMR206W_pPutative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication
YGL067W3.591.67E-4NPY1NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
YDR309C3.482.53E-4GIC2Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region

GO enrichment analysis for SGTC_1312
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0656.68E-7SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.0882353
0.0621.76E-6SGTC_2151287-0124 461.5 μMChemDiv (Drug-like library)55608630.148148
0.0621.92E-6SGTC_2525harmine 47.9 μMMiscellaneous52809530.0555556ergosterol biosynthesis
0.0552.58E-5SGTC_1066ketoconazole 628.0 nMNIH Clinical Collection4562010.0761905
0.0543.46E-5SGTC_7181130-0073 349.0 μMChemDiv (Drug-like library)67400200.140845
0.0543.55E-5SGTC_33299141233 27.6 μMChembridge (Drug-like library)49091010.0674157
0.0535.05E-5SGTC_9423253-1115 227.0 μMChemDiv (Drug-like library)464978970.103896
0.0535.23E-5SGTC_1927st060202 13.8 μMTimTec (Natural product derivative library)6888870.0441176
0.0501.05E-4SGTC_11740739-0097 51.1 μMChemDiv (Drug-like library)7284470.126761redox potentiating
0.0491.83E-4SGTC_6564090-1979 187.0 μMChemDiv (Drug-like library)223309610.0888889
0.0482.23E-4SGTC_12931000-1278 195.0 μMChemDiv (Drug-like library)6788470.15493
0.0473.10E-4SGTC_22176934799 96.1 μMChembridge (Fragment library)6756060.102941TSC3-RPN4
0.0454.80E-4SGTC_12971068-0111 14.1 μMChemDiv (Drug-like library)7670570.166667
0.0455.43E-4SGTC_14914138-0063 26.5 μMChemDiv (Drug-like library)238832270.0379747
0.0447.67E-4SGTC_7571066-0008 322.0 μMChemDiv (Drug-like library)68424970.2125Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6781218-217985.9 μM0.5090916820081ChemDiv (Drug-like library)272.256081.82515RNA pol III & RNase P/MRP
SGTC_9771348-1378135 μM0.430769X977ChemDiv (Drug-like library)362.342162.83982
SGTC_23955646831180.19 μM0.3888896808628Miscellaneous401.414664.7516
SGTC_659k018-0002107 μM0.3424666797879ChemDiv (Drug-like library)392.387981.97217
SGTC_597k018-000382.8 μM0.3378386786234ChemDiv (Drug-like library)370.382461.83717RNA pol III & RNase P/MRP
SGTC_9701315-008919.5 μM0.3285716811083ChemDiv (Drug-like library)582.172883.93523RSC & ERG11
SGTC_1642st00958277.7 μM0.3275866739311TimTec (Natural product derivative library)257.241441.48735RPP1 & pyrimidine depletion
SGTC_5660958-006816.5 μM0.3142866815365ChemDiv (Drug-like library)492.1358233.99424RSC & ERG11
SGTC_10451348-1485165 μM0.3108116810614ChemDiv (Drug-like library)396.787221.62127
SGTC_40960-023425.67 μM0.3098596821112ChemDiv (Drug-like library)558.108422.84424Golgi