1272-0050

(3Z)-1-benzyl-3-hydrazinylideneindol-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1316
Screen concentration 65.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 7340392
SMILES C1=CC=C(C=C1)CN2C3=CC=CC=C3C(=NN)C2=O
Standardized SMILES Oc1c(N=N)c2ccccc2n1Cc3ccccc3
Molecular weight 251.2832
ALogP 2.02
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.31
% growth inhibition (Hom. pool) 9.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 7340392
Download HIP data (tab-delimited text)  (excel)
Gene:CFT2(YLR115W)|FD-Score:3.73|P-value:9.70E-5|Clearance:0.33||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:GPI17(YDR434W)|FD-Score:-3.17|P-value:7.60E-4|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:GRS1(YBR121C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.14||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:MOT1(YPL082C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.13||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:PRP28(YDR243C)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:SEC14(YMR079W)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.02||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:UTP10(YJL109C)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YDR341C(YDR341C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.33||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CFT2(YLR115W)|FD-Score:3.73|P-value:9.70E-5|Clearance:0.33||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:GPI17(YDR434W)|FD-Score:-3.17|P-value:7.60E-4|Clearance:0||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:GRS1(YBR121C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.14||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:MOT1(YPL082C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.13||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:PRP28(YDR243C)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:SEC14(YMR079W)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.02||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:UTP10(YJL109C)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YDR341C(YDR341C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.33||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 7340392
Download HOP data (tab-delimited text)  (excel)
Gene:ALG5(YPL227C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:BDH1(YAL060W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CCW12(YLR110C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CWC15(YDR163W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:CYB5(YNL111C)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ERV14(YGL054C)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FDC1(YDR539W)|FD-Score:4.76|P-value:9.81E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:GAL10(YBR019C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GET2(YER083C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HXK1(YFR053C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IMH1(YLR309C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:MDM34(YGL219C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MMS1(YPR164W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MNN11(YJL183W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRF1(YGL143C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MSN1(YOL116W)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MTF2(YDL044C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NUP60(YAR002W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:NUR1(YDL089W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OGG1(YML060W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OPI11(YPR044C_d)|FD-Score:5.04|P-value:2.31E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PAC10(YGR078C)|FD-Score:-4.83|P-value:6.92E-7||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAN2(YGL094C)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PMP3(YDR276C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRR2(YDL214C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:QRI5(YLR204W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RLF2(YPR018W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:SDS3(YIL084C)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SEC22(YLR268W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEC66(YBR171W)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIN4(YNL236W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SPO71(YDR104C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:TMA64(YDR117C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:YAF9(YNL107W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBL083C(YBL083C_d)|FD-Score:4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YER121W(YER121W_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YGL041C(YGL041C_d)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL114W(YGL114W_p)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YLR063W(YLR063W_p)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:ZDS1(YMR273C)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication Gene:ALG5(YPL227C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:BDH1(YAL060W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CCW12(YLR110C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CWC15(YDR163W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:CYB5(YNL111C)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ERV14(YGL054C)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FDC1(YDR539W)|FD-Score:4.76|P-value:9.81E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:GAL10(YBR019C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GET2(YER083C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HXK1(YFR053C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IMH1(YLR309C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:MDM34(YGL219C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MMS1(YPR164W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MNN11(YJL183W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRF1(YGL143C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MSN1(YOL116W)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MTF2(YDL044C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NUP60(YAR002W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:NUR1(YDL089W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OGG1(YML060W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OPI11(YPR044C_d)|FD-Score:5.04|P-value:2.31E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PAC10(YGR078C)|FD-Score:-4.83|P-value:6.92E-7||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAN2(YGL094C)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PMP3(YDR276C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRR2(YDL214C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:QRI5(YLR204W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RLF2(YPR018W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:SDS3(YIL084C)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SEC22(YLR268W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEC66(YBR171W)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIN4(YNL236W)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SPO71(YDR104C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:TMA64(YDR117C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:YAF9(YNL107W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBL083C(YBL083C_d)|FD-Score:4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YER121W(YER121W_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YGL041C(YGL041C_d)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL114W(YGL114W_p)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YLR063W(YLR063W_p)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:ZDS1(YMR273C)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR115W3.739.70E-50.33CFT2Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
YDR341C3.671.20E-40.33YDR341CArginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPL082C3.354.08E-40.14MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YMR079W3.216.57E-40.02SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YBR121C3.197.06E-40.14GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YOR151C3.050.001150.20RPB2RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
YBR049C2.850.002190.18REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YOR160W2.670.003820.04MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YOR048C2.630.004300.03RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YDL028C2.590.004733.23E-4MPS1Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
YBR211C2.590.004740.02AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YKR071C2.570.005030.08DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YOR206W2.490.006320.04NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YGL207W2.460.007020.01SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YJL194W2.450.007160.01CDC6Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR044C_d5.042.31E-7OPI11_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO
YDR539W4.769.81E-7FDC1Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm
YBR171W4.721.20E-6SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YIL084C4.366.59E-6SDS3Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes
YGL041C_d4.191.40E-5YGL041C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR276C4.102.08E-5PMP3Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
YNL236W4.022.94E-5SIN4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
YBL083C_d4.003.21E-5YBL083C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
YJL183W3.904.76E-5MNN11Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
YOL116W3.787.97E-5MSN1Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YGL054C3.778.01E-5ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YPR164W3.768.53E-5MMS1Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
YGL094C3.759.00E-5PAN2Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
YNL111C3.749.23E-5CYB5Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
YML060W3.641.36E-4OGG1Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance

GO enrichment analysis for SGTC_1316
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0857.35E-11SGTC_32399133757 49.5 μMChembridge (Drug-like library)170369230.0555556
0.0841.20E-10SGTC_1875nocodazole 6.0 μMMiscellaneous41220.0684932tubulin folding & SWR complex
0.0831.91E-10SGTC_33309141424 71.4 μMChembridge (Drug-like library)272610200.0833333amide catabolism
0.0782.09E-9SGTC_7700389-1121 67.2 μMChemDiv (Drug-like library)67531080.0853659RPP1 & pyrimidine depletion
0.0764.24E-9SGTC_12310242-0792 125.0 μMChemDiv (Drug-like library)37076350.2
0.0663.24E-7SGTC_1766st045414 44.8 μMTimTec (Natural product derivative library)2539600.107692RPP1 & pyrimidine depletion
0.0663.86E-7SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.109091
0.0655.19E-7SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.103448Golgi
0.0656.01E-7SGTC_1679SEC14.276 6.7 μMMiscellaneous10050530.0714286Golgi
0.0649.85E-7SGTC_15153460-0031 42.1 μMChemDiv (Drug-like library)28765960.0921053DNA intercalators
0.0631.35E-6SGTC_11503909-7983 10.9 μMChemDiv (Drug-like library)239659140.0694444
0.0621.68E-6SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.133333RPP1 & pyrimidine depletion
0.0612.49E-6SGTC_31499097855 49.5 μMChembridge (Drug-like library)252366250.147541Golgi
0.0612.83E-6SGTC_31769106582 49.5 μMChembridge (Drug-like library)170180270.0769231
0.0612.85E-6SGTC_291SEC14.278 35.5 μMChemDiv (Drug-like library)10680440.126761Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_544k015-002052.4 μM0.53008304ChemDiv (Drug-like library)316.14943.20802
SGTC_5321548-0774215 μM0.4736845932993ChemDiv (Drug-like library)418.468322.56725
SGTC_1456k015-001213 μM0.4666672989616ChemDiv (Drug-like library)363.149873.03802
SGTC_1139k015-002446.9 μM0.43752766652ChemDiv (Drug-like library)305.251313.40205PDR1
SGTC_606k015-000838.6 μM0.41970475ChemDiv (Drug-like library)306.143463.78802
SGTC_6660272-0154136 μM0.3962266870312ChemDiv (Drug-like library)276.357342.41523cell wall
SGTC_14163966-032125.3 μM0.395349582530ChemDiv (Drug-like library)252.267981.8312
SGTC_20225136654200 μM0.3636361713771Chembridge (Fragment library)204.225180.99703
SGTC_1747st03749666.6 μM0.361702723305TimTec (Natural product derivative library)300.312540.64403
SGTC_1029k015-003064.7 μM0.362208316ChemDiv (Drug-like library)289.6888633.32903