1306-0155

N-(4-chlorophenyl)-5-methyl-4-nitro-1H-pyrazol-3-amine

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1319
Screen concentration 43.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 3122360
SMILES CC1=C(C(=NN1)NC2=CC=C(C=C2)Cl)[N+](=O)[O-]
Standardized SMILES Cc1[nH]nc(Nc2ccc(Cl)cc2)c1[N+](=O)[O-]
Molecular weight 252.6571
ALogP 3.07
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 27.77
% growth inhibition (Hom. pool) 13.96


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3122360
Download HIP data (tab-delimited text)  (excel)
Gene:CLP1(YOR250C)|FD-Score:-3.82|P-value:6.60E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:IRR1(YIL026C)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.1||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.19||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MIM1(YOL026C)|FD-Score:3.1|P-value:9.55E-4|Clearance:0.07||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NIC96(YFR002W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.07||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NUS1(YDL193W)|FD-Score:5.2|P-value:9.82E-8|Clearance:1.44||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PFY1(YOR122C)|FD-Score:3.76|P-value:8.40E-5|Clearance:0.18||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PRP2(YNR011C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.05||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RER2(YBR002C)|FD-Score:5.7|P-value:6.09E-9|Clearance:0.22||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLI1(YDR091C)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.02||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPB10(YOR210W)|FD-Score:5.73|P-value:5.17E-9|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL5(YPL131W)|FD-Score:5.48|P-value:2.13E-8|Clearance:0.28||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RRP45(YDR280W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RSP5(YER125W)|FD-Score:-3.77|P-value:8.32E-5|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SPC105(YGL093W)|FD-Score:9.84|P-value:3.88E-23|Clearance:4.11||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:YTH1(YPR107C)|FD-Score:3.11|P-value:9.37E-4|Clearance:0.01||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:CLP1(YOR250C)|FD-Score:-3.82|P-value:6.60E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:IRR1(YIL026C)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.1||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:KEI1(YDR367W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.19||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MIM1(YOL026C)|FD-Score:3.1|P-value:9.55E-4|Clearance:0.07||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NIC96(YFR002W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.07||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NUS1(YDL193W)|FD-Score:5.2|P-value:9.82E-8|Clearance:1.44||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PFY1(YOR122C)|FD-Score:3.76|P-value:8.40E-5|Clearance:0.18||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PRP2(YNR011C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.05||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RER2(YBR002C)|FD-Score:5.7|P-value:6.09E-9|Clearance:0.22||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLI1(YDR091C)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.02||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPB10(YOR210W)|FD-Score:5.73|P-value:5.17E-9|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL5(YPL131W)|FD-Score:5.48|P-value:2.13E-8|Clearance:0.28||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RRP45(YDR280W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RSP5(YER125W)|FD-Score:-3.77|P-value:8.32E-5|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SPC105(YGL093W)|FD-Score:9.84|P-value:3.88E-23|Clearance:4.11||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:YTH1(YPR107C)|FD-Score:3.11|P-value:9.37E-4|Clearance:0.01||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3122360
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM32(YML050W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARO8(YGL202W)|FD-Score:-5.61|P-value:1.01E-8||SGD DESC:Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis Gene:ATP14(YLR295C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP25(YMR098C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:ATP5(YDR298C)|FD-Score:7.61|P-value:1.38E-14||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AVT5(YBL089W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:-3.77|P-value:8.19E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM4(YPL161C)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CAF120(YNL278W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:COA2(YPL189C-A)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CSG2(YBR036C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTF19(YPL018W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:DSS1(YMR287C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EFG1(YGR271C-A)|FD-Score:4.9|P-value:4.78E-7||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FAB1(YFR019W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FMP33(YJL161W_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUI1(YBL042C)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GEP5(YLR091W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GTF1(YGR102C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HCR1(YLR192C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HMI1(YOL095C)|FD-Score:5.46|P-value:2.43E-8||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:KES1(YPL145C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAS21(YJL062W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MDG1(YNL173C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MDM12(YOL009C)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEF1(YLR069C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MMT1(YMR177W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MRM2(YGL136C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MRPL11(YDL202W)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL17(YNL252C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL22(YNL177C)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL40(YPL173W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS28(YDR337W)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH2(YOL090W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSM1(YGR171C)|FD-Score:4.3|P-value:8.42E-6||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MSS116(YDR194C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MST1(YKL194C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MSY1(YPL097W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NEW1(YPL226W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NUP60(YAR002W)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAC10(YGR078C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PCL2(YDL127W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth Gene:PER33(YLR064W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PIN4(YBL051C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPT2(YPL148C)|FD-Score:5.55|P-value:1.46E-8||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRD1(YCL057W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RAV2(YDR202C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RGP1(YDR137W)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RML2(YEL050C)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPN4(YDL020C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRG8(YPR116W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:RSM18(YER050C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM27(YGR215W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTK1(YDL025C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCM4(YGR049W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SEG2(YKL105C_p)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SIC1(YLR079W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SNF5(YBR289W)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SRB2(YHR041C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:STP22(YCL008C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SYS1(YJL004C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAN1(YGL232W)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:VMA3(YEL027W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS61(YDR136C_d)|FD-Score:12.5|P-value:2.24E-36||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR178W(YBR178W_d)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YDR114C(YDR114C_p)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR509W(YDR509W_d)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEH2(YLR020C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YGR219W(YGR219W_d)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YIL060W(YIL060W_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YLR149C(YLR149C_p)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR202C(YLR202C_d)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Putative protein of unknown function Gene:YME1(YPR024W)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YNL115C(YNL115C_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YPR064W(YPR064W_d)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRA2(YKL214C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YTP1(YNL237W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:AFG3(YER017C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM32(YML050W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARO8(YGL202W)|FD-Score:-5.61|P-value:1.01E-8||SGD DESC:Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis Gene:ATP14(YLR295C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP25(YMR098C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:ATP5(YDR298C)|FD-Score:7.61|P-value:1.38E-14||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AVT5(YBL089W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:-3.77|P-value:8.19E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM4(YPL161C)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CAF120(YNL278W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:COA2(YPL189C-A)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CSG2(YBR036C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTF19(YPL018W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:DSS1(YMR287C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EFG1(YGR271C-A)|FD-Score:4.9|P-value:4.78E-7||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FAB1(YFR019W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FMP33(YJL161W_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUI1(YBL042C)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GEP5(YLR091W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GTF1(YGR102C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HCR1(YLR192C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HMI1(YOL095C)|FD-Score:5.46|P-value:2.43E-8||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:KES1(YPL145C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAS21(YJL062W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MDG1(YNL173C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MDM12(YOL009C)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEF1(YLR069C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MMT1(YMR177W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MRM2(YGL136C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MRPL11(YDL202W)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL17(YNL252C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL22(YNL177C)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL40(YPL173W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS28(YDR337W)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH2(YOL090W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSM1(YGR171C)|FD-Score:4.3|P-value:8.42E-6||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MSS116(YDR194C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MST1(YKL194C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MSY1(YPL097W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NEW1(YPL226W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NUP60(YAR002W)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAC10(YGR078C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PCL2(YDL127W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth Gene:PER33(YLR064W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PIN4(YBL051C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPT2(YPL148C)|FD-Score:5.55|P-value:1.46E-8||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRD1(YCL057W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RAV2(YDR202C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RGP1(YDR137W)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RML2(YEL050C)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPN4(YDL020C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRG8(YPR116W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:RSM18(YER050C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM27(YGR215W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTK1(YDL025C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCM4(YGR049W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SEG2(YKL105C_p)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SIC1(YLR079W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SNF5(YBR289W)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SRB2(YHR041C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:STP22(YCL008C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SYS1(YJL004C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAN1(YGL232W)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:VMA3(YEL027W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS61(YDR136C_d)|FD-Score:12.5|P-value:2.24E-36||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR178W(YBR178W_d)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YDR114C(YDR114C_p)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR509W(YDR509W_d)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEH2(YLR020C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YGR219W(YGR219W_d)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YIL060W(YIL060W_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YLR149C(YLR149C_p)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR202C(YLR202C_d)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Putative protein of unknown function Gene:YME1(YPR024W)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YNL115C(YNL115C_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YPR064W(YPR064W_d)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRA2(YKL214C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YTP1(YNL237W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL093W9.843.88E-234.11SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YOR210W5.735.17E-90.03RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YBR002C5.706.09E-90.22RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YPL131W5.482.13E-80.28RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YDL193W5.209.82E-81.44NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YOR122C3.768.40E-50.18PFY1Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress
YFR002W3.581.71E-40.07NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YNR011C3.512.21E-40.05PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YIL026C3.462.70E-40.10IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDR367W3.363.86E-40.19KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YDR091C3.177.53E-40.02RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YDR280W3.167.98E-40.05RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YPR107C3.119.37E-40.01YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YOL026C3.109.55E-40.07MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YCR035C3.030.001210.07RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR136C_d12.502.24E-36VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YDR298C7.611.38E-14ATP5Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
YPL148C5.551.46E-8PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YOL095C5.462.43E-8HMI1Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
YDR137W5.161.25E-7RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YGR078C5.141.41E-7PAC10Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
YNL177C5.101.72E-7MRPL22Mitochondrial ribosomal protein of the large subunit
YGR271C-A4.904.78E-7EFG1Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
YBL042C4.875.67E-7FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YER050C4.631.81E-6RSM18Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
YLR064W4.611.98E-6PER33Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
YLR202C_d4.562.61E-6YLR202C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
YPL226W4.552.63E-6NEW1ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
YMR097C4.464.02E-6MTG1Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
YGR171C4.308.42E-6MSM1Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p

GO enrichment analysis for SGTC_1319
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2353.45E-75SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0909091TRP & mitochondrial translation
0.2247.16E-68SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.121622mitochondrial stress
0.2087.14E-59SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0615385mitochondrial stress
0.1783.75E-43SGTC_3101273-0058 1.1 μMChemDiv (Drug-like library)27342320.0344828mitochondrial stress
0.1779.07E-43SGTC_13181280-3875 124.0 μMChemDiv (Drug-like library)3732450.0612245
0.1722.68E-40SGTC_275trichlorophene 5.7 μMMiscellaneous626160.134615mitochondrial stress
0.1714.97E-40SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0909091TRP & mitochondrial translation
0.1718.35E-40SGTC_9083448-7491 47.7 μMChemDiv (Drug-like library)12030310.12069mitochondrial stress
0.1661.51E-37SGTC_11881403-0014 4.6 μMChemDiv (Drug-like library)96148670.112903mitochondrial stress
0.1651.74E-37SGTC_630099-0242 26.7 μMChemDiv (Drug-like library)38985020.0701754mitochondrial stress
0.1623.33E-36SGTC_2361509-0011 5.3 μMChemDiv (Drug-like library)31072800.15
0.1511.23E-31SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.0746269TRP & mitochondrial translation
0.1471.04E-29SGTC_5961155-0152 116.0 μMChemDiv (Drug-like library)2803130.235294TRP & mitochondrial translation
0.1352.21E-25SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.097561translation
0.1271.28E-22SGTC_1622st003707 19.4 μMTimTec (Natural product derivative library)101408990.0666667calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9671306-0166352 μM0.5652174437607ChemDiv (Drug-like library)246.265163.37524
SGTC_13591582-0056520 nM0.3103453661189ChemDiv (Drug-like library)304.688563.99416
SGTC_10124112-352218 μM0.2982463112398ChemDiv (Drug-like library)322.836243.81326
SGTC_9923909-8734162 μM0.2962964288319ChemDiv (Drug-like library)275.75664.01114
SGTC_1150527-0192112.04 μM0.2909094233931ChemDiv (Drug-like library)276.678464.14315
SGTC_5983937-023620.2 μM0.2807021379313ChemDiv (Drug-like library)354.80864.33515ERG2
SGTC_11891431-21197.28 μM0.283107275ChemDiv (Drug-like library)254.714244.76912
SGTC_1764st04450293.5 μM0.2765968407TimTec (Natural product derivative library)214.223.27413
SGTC_7342023-041780 μM0.2711863935732ChemDiv (Drug-like library)264.237381.79117ergosterol biosynthesis
SGTC_14314049-02609.09 μM0.2711863854841ChemDiv (Drug-like library)412.29033.93315