1348-1070

4-bromo-6-[[2-[(3,5-dimethyl-1H-pyrazol-4-yl)sulfanyl]anilino]methylidene]cyclohexa-2,4-dien-1-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1322
Screen concentration 12.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6758089
SMILES CC1=C(C(=NN1)C)SC2=CC=CC=C2NC=C3C=C(C=CC3=O)Br
Standardized SMILES Cc1n[nH]c(C)c1Sc2ccccc2N=Cc3cc(Br)ccc3O
Molecular weight 402.3081
ALogP 3.54
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.38
% growth inhibition (Hom. pool) -2.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6758089
Download HIP data (tab-delimited text)  (excel)
Gene:MCD4(YKL165C)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NUG1(YER006W)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.09||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PGA1(YNL158W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.42||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRP31(YGR091W)|FD-Score:4.04|P-value:2.65E-5|Clearance:0.6||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFA3(YJL173C)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.6||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:SRB6(YBR253W)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:TAF4(YMR005W)|FD-Score:4.39|P-value:5.58E-6|Clearance:0.6||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:YLR076C(YLR076C_d)|FD-Score:-3.51|P-value:2.20E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPL251W(YPL251W_d)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:MCD4(YKL165C)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NUG1(YER006W)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.09||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PGA1(YNL158W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.42||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRP31(YGR091W)|FD-Score:4.04|P-value:2.65E-5|Clearance:0.6||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFA3(YJL173C)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.6||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:SRB6(YBR253W)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:TAF4(YMR005W)|FD-Score:4.39|P-value:5.58E-6|Clearance:0.6||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:YLR076C(YLR076C_d)|FD-Score:-3.51|P-value:2.20E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPL251W(YPL251W_d)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6758089
Download HOP data (tab-delimited text)  (excel)
Gene:BBC1(YJL020C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:CAT2(YML042W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CDH1(YGL003C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COG7(YGL005C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX6(YHR051W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CYC1(YJR048W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DSS4(YPR017C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:FIG2(YCR089W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FUI1(YBL042C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HPA2(YPR193C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:IES2(YNL215W)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:MEP3(YPR138C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MFB1(YDR219C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MHP1(YJL042W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MMP1(YLL061W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MNT3(YIL014W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:PAU11(YGL261C_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PFK1(YGR240C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PKH1(YDR490C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PMS1(YNL082W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:SOD1(YJR104C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOH1(YGL127C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SSN8(YNL025C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SWD1(YAR003W)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SYG1(YIL047C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:THP1(YOL072W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPK1(YJL164C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UGA2(YBR006W)|FD-Score:4.34|P-value:7.01E-6||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VMA1(YDL185W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA5(YKL080W)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR419W(YLR419W_p)|FD-Score:4.31|P-value:8.25E-6||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR130W(YMR130W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:BBC1(YJL020C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:CAT2(YML042W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CDH1(YGL003C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COG7(YGL005C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX6(YHR051W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CYC1(YJR048W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:DSS4(YPR017C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:FIG2(YCR089W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FUI1(YBL042C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HPA2(YPR193C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:IES2(YNL215W)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:MEP3(YPR138C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MFB1(YDR219C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MHP1(YJL042W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MMP1(YLL061W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MNT3(YIL014W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:PAU11(YGL261C_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PFK1(YGR240C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PKH1(YDR490C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PMS1(YNL082W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:SOD1(YJR104C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOH1(YGL127C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SSN8(YNL025C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SWD1(YAR003W)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SYG1(YIL047C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:THP1(YOL072W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPK1(YJL164C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UGA2(YBR006W)|FD-Score:4.34|P-value:7.01E-6||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VMA1(YDL185W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA5(YKL080W)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR419W(YLR419W_p)|FD-Score:4.31|P-value:8.25E-6||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR130W(YMR130W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR005W4.395.58E-60.60TAF4TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
YJL173C4.092.20E-50.60RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YGR091W4.042.65E-50.60PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YER006W3.442.91E-40.09NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YBR253W3.354.05E-40.02SRB6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YPL251W_d3.334.41E-40.01YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YNL158W3.314.65E-40.42PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YLR272C2.890.001920.05YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YLR103C2.850.002210.06CDC45DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
YPR175W2.780.002710.03DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YDR454C2.750.002960.03GUK1Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
YGR074W2.720.003240.04SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YGR114C_d2.680.003650.07YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YIL083C2.610.004550.09CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YML064C2.520.005850.09TEM1GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL042C5.822.91E-9FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YBR006W4.347.01E-6UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YLR419W_p4.318.25E-6YLR419W_pPutative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
YDR490C4.151.69E-5PKH1Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p
YOR385W_p3.807.22E-5YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YGL186C3.787.75E-5TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YJR048W3.729.86E-5CYC1Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication
YMR130W_p3.671.21E-4YMR130W_pPutative protein of unknown function; YMR130W is not an essential gene
YHR051W3.651.32E-4COX6Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
YNL082W3.641.35E-4PMS1ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
YJL020C3.631.42E-4BBC1Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
YJR104C3.601.57E-4SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YIL014W3.581.72E-4MNT3Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation
YJL164C3.571.76E-4TPK1cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
YPR017C3.532.11E-4DSS4Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol

GO enrichment analysis for SGTC_1322
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0808.19E-10SGTC_15258-methoxypsoralen 92.5 μMTimTec (Pure natural product library)41140.0641026DNA damage response
0.0723.36E-8SGTC_14863970-0793 4.5 μMChemDiv (Drug-like library)28443300.101449
0.0715.22E-8SGTC_1083didemethylchlorpromazine sulfoxide 707.4 nMTimTec (Natural product library)3987880.0487805redox potentiating
0.0706.39E-8SGTC_15773(2'-chlorophenyl)-7-ethoxycoumarin 66.5 μMTimTec (Pure natural product library)6890070.0843373
0.0681.61E-7SGTC_230MPP+ 141.7 μMMiscellaneous394840.101695
0.0672.27E-7SGTC_1033k072-0102 21.3 μMChemDiv (Drug-like library)59659310.160S ribosome export
0.0672.97E-7SGTC_1799st045822 46.1 μMTimTec (Natural product derivative library)36749920.0612245
0.0655.68E-7SGTC_1078idebenone 36.1 μMMiscellaneous36860.0547945Golgi
0.0631.23E-6SGTC_5991935-0052 86.5 μMChemDiv (Drug-like library)95573110.1
0.0621.91E-6SGTC_3883',4'-dichloroisocoumarin 16.7 μMMiscellaneous16090.0769231
0.0603.69E-6SGTC_20805212524 12.4 μMChembridge (Fragment library)2601830.0909091copper-dependent oxidative stress
0.0604.22E-6SGTC_29679089538 49.9 μMChembridge (Drug-like library)7282160.0731707
0.0604.28E-6SGTC_9611252-1571 372.0 μMChemDiv (Drug-like library)68134980.360656
0.0571.23E-5SGTC_10234482-3888 16.0 μMChemDiv (Drug-like library)33031680.0759494TSC3-RPN4
0.0571.27E-5SGTC_13341486-0610 236.0 μMChemDiv (Drug-like library)37423390.045454560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3301488-031980.1 μM0.4531256846614ChemDiv (Drug-like library)392.248663.99223RPP1 & pyrimidine depletion
SGTC_1320417-171525.81 μM0.4307696769098ChemDiv (Drug-like library)409.299024.56114RPP1 & pyrimidine depletion
SGTC_7560508-199928.3 μM0.4307696753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_13531498-12068.11 μM0.379316756808ChemDiv (Drug-like library)371.024062.76223mitochondrial response to ROS
SGTC_3031348-166834.2 μM0.3709685380509ChemDiv (Drug-like library)292.13131.61324
SGTC_310508-197457.24 μM0.3676476810525ChemDiv (Drug-like library)414.679724.6213
SGTC_9611252-1571372 μM0.3606566813498ChemDiv (Drug-like library)288.16421.05124
SGTC_910417-175158.38 μM0.3571436795624ChemDiv (Drug-like library)409.299024.56114Golgi
SGTC_12700831-117017.3 μM0.3538466764716ChemDiv (Drug-like library)380.234664.25113
SGTC_329k048-012121.3 μM0.3442626832902ChemDiv (Drug-like library)480.921193.58212RSC & ERG11