1348-1351

N-[(Z)-(5-nitrofuran-2-yl)methylideneamino]-N'-pyridin-2-ylpyridine-2-carboximidamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1323
Screen concentration 11.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 6989654
SMILES C1=CC=NC(=C1)C(=NC2=CC=CC=N2)NN=CC3=CC=C(O3)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1oc(C=NN=C(Nc2ccccn2)c3ccccn3)cc1
Molecular weight 336.3049
ALogP 2.6
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.55
% growth inhibition (Hom. pool) 3.1


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6989654
Download HIP data (tab-delimited text)  (excel)
Gene:MED6(YHR058C)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:RPB5(YBR154C)|FD-Score:5.34|P-value:4.70E-8|Clearance:0.96||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:TFB4(YPR056W)|FD-Score:4.38|P-value:6.00E-6|Clearance:0.49||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:UTP13(YLR222C)|FD-Score:3.89|P-value:5.05E-5|Clearance:0.09||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP25(YIL091C)|FD-Score:3.8|P-value:7.35E-5|Clearance:0.73||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YLR076C(YLR076C_d)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:MED6(YHR058C)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:RPB5(YBR154C)|FD-Score:5.34|P-value:4.70E-8|Clearance:0.96||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:TFB4(YPR056W)|FD-Score:4.38|P-value:6.00E-6|Clearance:0.49||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:UTP13(YLR222C)|FD-Score:3.89|P-value:5.05E-5|Clearance:0.09||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP25(YIL091C)|FD-Score:3.8|P-value:7.35E-5|Clearance:0.73||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YLR076C(YLR076C_d)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6989654
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:CHS5(YLR330W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CYB5(YNL111C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:FRE8(YLR047C)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GYP1(YOR070C)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAC1(YFL031W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:IRC9(YJL142C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LOS1(YKL205W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:LYS5(YGL154C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine Gene:MAC1(YMR021C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MLS1(YNL117W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL15(YLR312W-A)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:PDC6(YGR087C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PHO80(YOL001W)|FD-Score:3.72|P-value:9.97E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PKR1(YMR123W)|FD-Score:-3.94|P-value:4.08E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POR1(YNL055C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RAD5(YLR032W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAS2(YNL098C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:REF2(YDR195W)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RGL1(YPL066W)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL15B(YMR121C)|FD-Score:3.77|P-value:8.33E-5||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SMI1(YGR229C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF6(YHL025W)|FD-Score:-4.46|P-value:4.07E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPG5(YMR191W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SSN2(YDR443C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TDA1(YMR291W)|FD-Score:-4.84|P-value:6.40E-7||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UBP9(YER098W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VMA9(YCL005W-A)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS21(YOR089C)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YAK1(YJL141C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR016W(YBR016W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDR524W-C(YDR524W-C_p)|FD-Score:-4.44|P-value:4.51E-6||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YER091C-A(YER091C-A_d)|FD-Score:4.91|P-value:4.65E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR366W(YLR366W_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML012C-A(YML012C-A_d)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YNL108C(YNL108C_p)|FD-Score:5.31|P-value:5.53E-8||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOL046C(YOL046C_d)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR072W(YOR072W_d)|FD-Score:-4.18|P-value:1.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR277C(YOR277C_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPQ1(YOL092W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:ADE1(YAR015W)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:CHS5(YLR330W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CYB5(YNL111C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:FRE8(YLR047C)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GYP1(YOR070C)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAC1(YFL031W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:IRC9(YJL142C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LOS1(YKL205W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:LYS5(YGL154C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine Gene:MAC1(YMR021C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MLS1(YNL117W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL15(YLR312W-A)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:PDC6(YGR087C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PHO80(YOL001W)|FD-Score:3.72|P-value:9.97E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PKR1(YMR123W)|FD-Score:-3.94|P-value:4.08E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POR1(YNL055C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RAD5(YLR032W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAS2(YNL098C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:REF2(YDR195W)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RGL1(YPL066W)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL15B(YMR121C)|FD-Score:3.77|P-value:8.33E-5||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SMI1(YGR229C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF6(YHL025W)|FD-Score:-4.46|P-value:4.07E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPG5(YMR191W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SSN2(YDR443C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TDA1(YMR291W)|FD-Score:-4.84|P-value:6.40E-7||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UBP9(YER098W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VMA9(YCL005W-A)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS21(YOR089C)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YAK1(YJL141C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR016W(YBR016W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDR524W-C(YDR524W-C_p)|FD-Score:-4.44|P-value:4.51E-6||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YER091C-A(YER091C-A_d)|FD-Score:4.91|P-value:4.65E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR366W(YLR366W_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML012C-A(YML012C-A_d)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YNL108C(YNL108C_p)|FD-Score:5.31|P-value:5.53E-8||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOL046C(YOL046C_d)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR072W(YOR072W_d)|FD-Score:-4.18|P-value:1.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR277C(YOR277C_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPQ1(YOL092W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR154C5.344.70E-80.96RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YPR056W4.386.00E-60.49TFB4Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
YLR222C3.895.05E-50.09UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YIL091C3.807.35E-50.73UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YHR062C3.070.001090.02RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR246C3.040.001160.08BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YBL084C2.970.001500.01CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YJL173C2.960.001520.03RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YHR069C2.930.001670.01RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YOR218C_d2.920.001730.02YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
YPR016C2.910.001830.07TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YOR340C2.840.002250.08RPA43RNA polymerase I subunit A43
YDR002W2.760.002900.11YRB1Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
YOL139C2.650.004030.01CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YKL082C2.640.004090.00RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL108C_p5.315.53E-8YNL108C_pPutative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
YER091C-A_d4.914.65E-7YER091C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR106W4.671.48E-6VAM3Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
YOR089C4.279.81E-6VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YJL141C4.121.88E-5YAK1Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose
YMR191W4.022.86E-5SPG5Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
YAR015W3.924.35E-5ADE1N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
YBR016W3.904.85E-5YBR016WTail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
YLR047C3.894.96E-5FRE8Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p
YNL098C3.875.41E-5RAS2GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
YLR032W3.865.59E-5RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YMR121C3.778.33E-5RPL15BRibosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
YOL001W3.729.97E-5PHO80Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
YMR021C3.671.23E-4MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YOL092W_p3.661.26E-4YPQ1_pPutative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter

GO enrichment analysis for SGTC_1323
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1024.06E-15SGTC_32299132204 49.5 μMChembridge (Drug-like library)176829240.0978261
0.0881.58E-11SGTC_11921488-0095 21.4 μMChemDiv (Drug-like library)9158750.0759494
0.0781.91E-9SGTC_29299013348 24.6 μMChembridge (Drug-like library)29959520.105263
0.0741.31E-8SGTC_11443486-0183 12.5 μMChemDiv (Drug-like library)67440690.0506329
0.0731.84E-8SGTC_2571chrysanthemic acid 100.0 μMMicrosource (Natural product library)27430.0441176
0.0722.73E-8SGTC_11841269-1718 139.0 μMChemDiv (Drug-like library)54038210.131579
0.0631.33E-6SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.123077DNA damage response
0.0612.39E-6SGTC_29479072639 7.7 μMChembridge (Drug-like library)164881460.0898876tubulin folding & SWR complex
0.0612.88E-6SGTC_29429052777 62.2 μMChembridge (Drug-like library)64695030.108108
0.0595.84E-6SGTC_14464239-0190 62.0 μMChemDiv (Drug-like library)13400850.1
0.0587.39E-6SGTC_12560570-0116 285.0 μMChemDiv (Drug-like library)4106380.0266667
0.0587.83E-6SGTC_220964-0034 14.5 μMChemDiv (Drug-like library)1384020.0298507unfolded protein response
0.0571.18E-5SGTC_12660828-0227 32.9 μMChemDiv (Drug-like library)39138200.0972222
0.0561.75E-5SGTC_26092,3,4-trihydroxy-4'-ethoxybenzophenone 70.0 μMMicrosource (Natural product library)67087390.0657895redox potentiating
0.0561.80E-5SGTC_11513909-8316 15.3 μMChemDiv (Drug-like library)8004310.0731707

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9771348-1378135 μM0.606557X977ChemDiv (Drug-like library)362.342162.83982
SGTC_10451348-1485165 μM0.5692316810614ChemDiv (Drug-like library)396.787221.62127
SGTC_6221348-156626.3 μM0.543867340407ChemDiv (Drug-like library)370.235324.51514
SGTC_12181348-141630.7 μM0.4153856787668ChemDiv (Drug-like library)342.354061.61925SWF1 & branched chain AA biosynthesis
SGTC_7531322-0200289 μM0.406255403907ChemDiv (Drug-like library)275.216961.85526
SGTC_9473447-0010211 μM0.31746748350ChemDiv (Drug-like library)238.24142.59813
SGTC_2131246-0773967 nM0.3150686251622ChemDiv (Drug-like library)323.325963.87806
SGTC_5504123-038525.7 μM0.301375731123ChemDiv (Drug-like library)311.292122.9305TSC3-RPN4
SGTC_1700st0308407.93 μM0.2962965499307TimTec (Natural product derivative library)359.333382.92417PDR1
SGTC_9031134-0042114 μM0.293103701009ChemDiv (Drug-like library)229.300843.75522