1436-0082

3-(4-bromobenzoyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1327
Screen concentration 2.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 15992205
SMILES C1=CC=C2C(=C1)C(=O)C(=CO2)C(=O)C3=CC=C(C=C3)Br
Standardized SMILES Brc1ccc(cc1)C(=O)C2=COc3ccccc3C2=O
Molecular weight 329.1449
ALogP 3.5
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.64
% growth inhibition (Hom. pool) 9.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 15992205
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.72|P-value:9.86E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC39(YCR093W)|FD-Score:-3.52|P-value:2.16E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DBF4(YDR052C)|FD-Score:-4|P-value:3.15E-5|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DOP1(YDR141C)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.32||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERG11(YHR007C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.06||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG29(YMR134W_p)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.01||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:HSH49(YOR319W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRR1(YIL026C)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.03||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NOC2(YOR206W)|FD-Score:-3.87|P-value:5.39E-5|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP58(YOR310C)|FD-Score:3.4|P-value:3.37E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUG1(YER006W)|FD-Score:-3.21|P-value:6.57E-4|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PRE10(YOR362C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.02||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE4(YFR050C)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.15||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE6(YOL038W)|FD-Score:3.99|P-value:3.37E-5|Clearance:0.29||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PSA1(YDL055C)|FD-Score:-3.15|P-value:8.15E-4|Clearance:0||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:RPB5(YBR154C)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPN3(YER021W)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.04||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:8.59|P-value:4.28E-18|Clearance:3.85||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.15||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SEC13(YLR208W)|FD-Score:4.1|P-value:2.03E-5|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:10|P-value:6.06E-24|Clearance:3.85||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TSR1(YDL060W)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.05||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YGR115C(YGR115C_d)|FD-Score:4.36|P-value:6.50E-6|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YPT1(YFL038C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.36||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:YRA1(YDR381W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.06||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:CCT3(YJL014W)|FD-Score:-3.72|P-value:9.86E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC39(YCR093W)|FD-Score:-3.52|P-value:2.16E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DBF4(YDR052C)|FD-Score:-4|P-value:3.15E-5|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DOP1(YDR141C)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.32||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERG11(YHR007C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.06||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG29(YMR134W_p)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.01||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:HSH49(YOR319W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRR1(YIL026C)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.03||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NOC2(YOR206W)|FD-Score:-3.87|P-value:5.39E-5|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP58(YOR310C)|FD-Score:3.4|P-value:3.37E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUG1(YER006W)|FD-Score:-3.21|P-value:6.57E-4|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PRE10(YOR362C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.02||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE4(YFR050C)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.15||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE6(YOL038W)|FD-Score:3.99|P-value:3.37E-5|Clearance:0.29||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PSA1(YDL055C)|FD-Score:-3.15|P-value:8.15E-4|Clearance:0||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:RPB5(YBR154C)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPN3(YER021W)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.04||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:8.59|P-value:4.28E-18|Clearance:3.85||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.15||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SEC13(YLR208W)|FD-Score:4.1|P-value:2.03E-5|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:10|P-value:6.06E-24|Clearance:3.85||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TSR1(YDL060W)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.05||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YGR115C(YGR115C_d)|FD-Score:4.36|P-value:6.50E-6|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YPT1(YFL038C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.36||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:YRA1(YDR381W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.06||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 15992205
Download HOP data (tab-delimited text)  (excel)
Gene:AIM23(YJL131C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:ALR2(YFL050C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:ARL1(YBR164C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG34(YOL083W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:BCK1(YJL095W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDS1(YOL164W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BIO2(YGR286C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BUD19(YJL188C_d)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBS1(YDL069C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:COG5(YNL051W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:5.78|P-value:3.80E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:7.08|P-value:7.39E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX8(YLR395C)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUE2(YKL090W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination Gene:DHH1(YDL160C)|FD-Score:10.3|P-value:2.48E-25||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FYV6(YNL133C)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GLN3(YER040W)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GYP1(YOR070C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBT1(YDL223C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HPA3(YEL066W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:HTA1(YDR225W)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IRS4(YKR019C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LSM6(YDR378C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MDM20(YOL076W)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MET10(YFR030W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MSH3(YCR092C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC6(YHR151C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTC7(YEL033W_p)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NBP2(YDR162C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PUF2(YPR042C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RPL34B(YIL052C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL36B(YPL249C-A)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RSM22(YKL155C)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTC6(YPL183W-A)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT101(YJL047C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RXT3(YDL076C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SEC22(YLR268W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:STP22(YCL008C)|FD-Score:5.3|P-value:5.81E-8||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWI6(YLR182W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SXM1(YDR395W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYS1(YJL004C)|FD-Score:7.46|P-value:4.26E-14||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TED1(YIL039W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRS65(YGR166W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:URE2(YNL229C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS51(YKR020W)|FD-Score:6.8|P-value:5.35E-12||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS62(YGR141W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:VPS63(YLR261C_d)|FD-Score:5.37|P-value:4.03E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS65(YLR322W_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAL065C(YAL065C_p)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR206W(YBR206W_d)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YBR287W(YBR287W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL041W(YDL041W_d)|FD-Score:4.79|P-value:8.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR476C(YDR476C_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YML079W(YML079W_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YPR196W(YPR196W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YPT7(YML001W)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:AIM23(YJL131C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:ALR2(YFL050C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:ARL1(YBR164C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG34(YOL083W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:BCK1(YJL095W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDS1(YOL164W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BIO2(YGR286C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BUD19(YJL188C_d)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBS1(YDL069C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:COG5(YNL051W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:5.78|P-value:3.80E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:7.08|P-value:7.39E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX8(YLR395C)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUE2(YKL090W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination Gene:DHH1(YDL160C)|FD-Score:10.3|P-value:2.48E-25||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FYV6(YNL133C)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GLN3(YER040W)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GYP1(YOR070C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBT1(YDL223C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HPA3(YEL066W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:HTA1(YDR225W)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IRS4(YKR019C)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LSM6(YDR378C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MDM20(YOL076W)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MET10(YFR030W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MSH3(YCR092C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC6(YHR151C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTC7(YEL033W_p)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NBP2(YDR162C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PUF2(YPR042C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RPL34B(YIL052C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL36B(YPL249C-A)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RSM22(YKL155C)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTC6(YPL183W-A)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT101(YJL047C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RXT3(YDL076C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SEC22(YLR268W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:STP22(YCL008C)|FD-Score:5.3|P-value:5.81E-8||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWI6(YLR182W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SXM1(YDR395W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYS1(YJL004C)|FD-Score:7.46|P-value:4.26E-14||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TED1(YIL039W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRS65(YGR166W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:URE2(YNL229C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS51(YKR020W)|FD-Score:6.8|P-value:5.35E-12||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS62(YGR141W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:VPS63(YLR261C_d)|FD-Score:5.37|P-value:4.03E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS65(YLR322W_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAL065C(YAL065C_p)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR206W(YBR206W_d)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YBR287W(YBR287W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL041W(YDL041W_d)|FD-Score:4.79|P-value:8.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR476C(YDR476C_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YML079W(YML079W_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YPR196W(YPR196W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YPT7(YML001W)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W10.006.06E-243.85SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YHR062C8.594.28E-183.85RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR362C4.751.03E-60.02PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YFL038C4.721.17E-60.36YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YGR115C_d4.366.50E-60.22YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YER021W4.141.74E-50.04RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YLR208W4.102.03E-50.08SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR154C4.032.84E-50.04RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YOL038W3.993.37E-50.29PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YDR381W3.691.11E-40.06YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YHR007C3.631.41E-40.06ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YLR167W3.571.77E-40.15RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YIL026C3.433.07E-40.03IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YOR310C3.403.37E-40.03NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YMR134W_p3.373.72E-40.01ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL160C10.302.48E-25DHH1Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
YJL004C7.464.26E-14SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YGL005C7.087.39E-13COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YKR020W6.805.35E-12VPS51Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
YNL041C5.783.80E-9COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YKR019C5.571.24E-8IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YLR261C_d5.374.03E-8VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YCL008C5.305.81E-8STP22Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
YDL041W_d4.798.46E-7YDL041W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
YER040W4.751.00E-6GLN3Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source
YJL188C_d4.612.03E-6BUD19_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YIL039W4.483.65E-6TED1Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YPL051W4.444.52E-6ARL3GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
YOL076W4.425.04E-6MDM20Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
YLR268W4.211.29E-5SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog

GO enrichment analysis for SGTC_1327
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2525.23E-86SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.396226
0.2478.12E-83SGTC_389tpck 16.7 μMMiscellaneous4396470.123077
0.2465.41E-82SGTC_8780828-0289 13.7 μMChemDiv (Drug-like library)7453500.423077
0.2363.36E-75SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.106667
0.2311.78E-72SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0933333Golgi
0.2274.99E-70SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.0945946
0.2263.50E-69SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0952381
0.2201.90E-65SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.185185Golgi
0.2202.13E-65SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.125Golgi
0.2195.12E-65SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0819672Golgi
0.2104.99E-60SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.0526316Golgi
0.2072.12E-58SGTC_8550428-0020 25.0 μMChemDiv (Drug-like library)68124140.107143Golgi
0.1962.92E-52SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.161765RPP1 & pyrimidine depletion
0.1843.51E-46SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0350877Golgi
0.1845.01E-46SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.233333Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12680828-02752.46 μM0.74359745337ChemDiv (Drug-like library)284.693863.41603
SGTC_770828-028612.52 μM0.555556745345ChemDiv (Drug-like library)264.275383.23803TSC3-RPN4
SGTC_12670828-02729.49 μM0.545455745334ChemDiv (Drug-like library)278.301963.72403
SGTC_8780828-028913.7 μM0.423077745350ChemDiv (Drug-like library)298.720443.90303
SGTC_1724st0372818.29 μM0.396226574124TimTec (Natural product derivative library)270.30313.19204
SGTC_23945221648200 μM0.391304788586Miscellaneous346.132322.90504
SGTC_1693xanthone101.9 μM0.3684217020TimTec (Natural product derivative library)196.201422.96402DNA damage response
SGTC_2532xanthone78.9 μM0.3684217020Microsource (Natural product library)196.201422.96402
SGTC_1684st02453814.62 μM0.3571436732859TimTec (Natural product derivative library)277.29574-1.5726
SGTC_1060092-000311.58 μM0.3541671072148ChemDiv (Drug-like library)355.182143.67503copper-dependent oxidative stress
SGTC_350133-005464.07 μM0.31914999677ChemDiv (Drug-like library)276.12863.23512