1441-0025

1-(4-nitrophenyl)-3-phenylmethoxyurea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1328
Screen concentration 69.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 4562601
SMILES C1=CC=C(C=C1)CONC(=O)NC2=CC=C(C=C2)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(NC(=O)NOCc2ccccc2)cc1
Molecular weight 287.2707
ALogP 2.27
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.01
% growth inhibition (Hom. pool) 8.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4562601
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:4.49|P-value:3.59E-6|Clearance:0.07||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ARP4(YJL081C)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CCT3(YJL014W)|FD-Score:-3.86|P-value:5.58E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:PRP45(YAL032C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.12||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RBA50(YDR527W)|FD-Score:-3.34|P-value:4.15E-4|Clearance:0||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RER2(YBR002C)|FD-Score:6.14|P-value:4.24E-10|Clearance:1.65||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSE1(YML049C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.08||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:TAF4(YMR005W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.33||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:UBC1(YDR177W)|FD-Score:4.42|P-value:5.03E-6|Clearance:0.73||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UFD1(YGR048W)|FD-Score:3.16|P-value:7.92E-4|Clearance:0.11||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:ABF1(YKL112W)|FD-Score:4.49|P-value:3.59E-6|Clearance:0.07||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ARP4(YJL081C)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CCT3(YJL014W)|FD-Score:-3.86|P-value:5.58E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:PRP45(YAL032C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.12||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RBA50(YDR527W)|FD-Score:-3.34|P-value:4.15E-4|Clearance:0||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RER2(YBR002C)|FD-Score:6.14|P-value:4.24E-10|Clearance:1.65||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSE1(YML049C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.08||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:TAF4(YMR005W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.33||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:UBC1(YDR177W)|FD-Score:4.42|P-value:5.03E-6|Clearance:0.73||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UFD1(YGR048W)|FD-Score:3.16|P-value:7.92E-4|Clearance:0.11||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4562601
Download HOP data (tab-delimited text)  (excel)
Gene:BUD23(YCR047C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CDC26(YFR036W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CSM4(YPL200W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DAL3(YIR032C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:ECM8(YBR076W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Non-essential protein of unknown function Gene:FAR3(YMR052W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FIT2(YOR382W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GSC2(YGR032W)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HBT1(YDL223C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOT1(YMR172W)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:HSL7(YBR133C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HUA1(YGR268C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:KAR9(YPL269W)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KTI11(YBL071W-A)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LSB6(YJL100W)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MET12(YPL023C)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MMM1(YLL006W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL11(YDL202W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:OSH2(YDL019C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PUF6(YDR496C)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RPS6B(YBR181C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SCW10(YMR305C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:TAT2(YOL020W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TPK2(YPL203W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VAC7(YNL054W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAC8(YEL013W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBP2(YGL060W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL062W(YDL062W_d)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDR061W(YDR061W_p)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR537C(YDR537C_d)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEL028W(YEL028W_d)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YOR199W(YOR199W_d)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL260W(YPL260W_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YSR3(YKR053C)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:BUD23(YCR047C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CDC26(YFR036W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CSM4(YPL200W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DAL3(YIR032C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:ECM8(YBR076W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Non-essential protein of unknown function Gene:FAR3(YMR052W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FIT2(YOR382W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GSC2(YGR032W)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HBT1(YDL223C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOT1(YMR172W)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:HSL7(YBR133C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HUA1(YGR268C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:KAR9(YPL269W)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KTI11(YBL071W-A)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LSB6(YJL100W)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MET12(YPL023C)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MMM1(YLL006W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL11(YDL202W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:OSH2(YDL019C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PUF6(YDR496C)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RPS6B(YBR181C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SCW10(YMR305C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:TAT2(YOL020W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TPK2(YPL203W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VAC7(YNL054W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAC8(YEL013W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBP2(YGL060W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL062W(YDL062W_d)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDR061W(YDR061W_p)|FD-Score:-3.33|P-value:4.42E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR537C(YDR537C_d)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEL028W(YEL028W_d)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YOR199W(YOR199W_d)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL260W(YPL260W_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YSR3(YKR053C)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C6.144.24E-101.65RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YKL112W4.493.59E-60.07ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YDR177W4.425.03E-60.73UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YAL032C3.691.14E-40.12PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YMR005W3.561.84E-40.33TAF4TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
YML049C3.246.04E-40.08RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YGR048W3.167.92E-40.11UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YJR112W3.050.001140.22NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YOR294W2.830.002330.06RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YPR108W2.770.002835.43E-4RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YHR072W2.770.002830.01ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YHR024C2.760.002920.09MAS2Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YDR045C2.670.003790.00RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YBR140C2.670.003810.12IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YDR145W2.540.005480.04TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL269W4.473.88E-6KAR9Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
YKR053C4.464.18E-6YSR3Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication
YGR290W_d4.347.07E-6YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YEL013W4.221.21E-5VAC8Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
YOL020W4.102.03E-5TAT2High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance
YCR047C4.102.03E-5BUD23Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
YDL202W4.003.23E-5MRPL11Mitochondrial ribosomal protein of the large subunit
YDR181C3.914.57E-5SAS4Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
YBR133C3.836.44E-5HSL7Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress
YBL071W-A3.749.24E-5KTI11Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
YBR196C-A_p3.711.05E-4YBR196C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YBR076W3.681.15E-4ECM8Non-essential protein of unknown function
YBR181C3.522.14E-4RPS6BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
YGL186C3.462.72E-4TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YGL188C-A_p3.452.85E-4YGL188C-A_pPutative protein of unknown function

GO enrichment analysis for SGTC_1328
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0944.50E-13SGTC_1863455-3465 67.3 μMChemDiv (Drug-like library)7552780.0806452
0.0897.16E-12SGTC_28899059656 52.0 μMChembridge (Drug-like library)39498380.169492
0.0847.87E-11SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0508475TRP & mitochondrial translation
0.0823.04E-10SGTC_2516gossypol 71.5 μMTimTec (Pure natural product library)35030.0447761
0.0809.26E-10SGTC_5203584-0023 682.0 μMChemDiv (Drug-like library)13407900.102941
0.0741.33E-8SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0508475TRP & mitochondrial translation
0.0723.41E-8SGTC_1121fusaric acid 630.5 nMTimTec (Natural product library)34420.135593
0.0714.34E-8SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.104478TRP & mitochondrial translation
0.0691.07E-7SGTC_23327982454 138.9 μMChembridge (Fragment library)29817480.117647
0.0664.12E-7SGTC_8490386-0493 241.0 μMChemDiv (Drug-like library)7261190.109091
0.0664.24E-7SGTC_13181280-3875 124.0 μMChemDiv (Drug-like library)3732450.102041
0.0655.97E-7SGTC_2664lyso-paf C-16 73.5 μMMicrosource (Natural product library)67107420.0659341
0.0649.35E-7SGTC_772k072-0172 35.3 μMChemDiv (Drug-like library)58619800.084507
0.0649.85E-7SGTC_204PMA1.184 62.4 μMChemDiv (Drug-like library)22522130.103896TRP & mitochondrial translation
0.0622.05E-6SGTC_2513baicalein 25.6 μMMicrosource (Natural product library)52816050.111111calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_850868-0259160.06 μM0.60465183173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_990109-0120622.8 μM0.416667759414ChemDiv (Drug-like library)321.126163.25713
SGTC_21836420625130.51 μM0.3818182897619Chembridge (Fragment library)277.299043.17124mitochondrial processes
SGTC_9262268-140159.4 μM0.379312931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_22727805186180 μM0.3773582956062Chembridge (Fragment library)268.335342.54524cell wall signaling
SGTC_1778st04681958.9 μM0.370968671715TimTec (Natural product derivative library)339.345283.69324
SGTC_750159-0059199.01 μM0.370375377270ChemDiv (Drug-like library)251.238621.99916fatty acid desaturase (OLE1)
SGTC_9491165-0505146 μM0.3653856244267ChemDiv (Drug-like library)304.321082.15415
SGTC_458nppb133 μM0.3620694549ICCB bioactive library300.309243.52325
SGTC_1813st0512468.6 μM0.365291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4