1469-0054

N-[(Z)-[3-[(2-chlorophenyl)hydrazinylidene]-6-oxocyclohexa-1,4-dien-1-yl]methylideneamino]-5-methyl-1H-pyrazole-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1330
Screen concentration 61.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 7102664
SMILES CC1=CC(=NN1)C(=O)NN=CC2=CC(=NNC3=CC=CC=C3Cl)C=CC2=O
Standardized SMILES Cc1cc([nH]n1)C(=O)NN=Cc2cc(ccc2O)N=Nc3ccccc3Cl
Molecular weight 382.8037
ALogP 2.94
H-bond donor count 3
H-bond acceptor count 6
Response signature iron homeostasis

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.13
% growth inhibition (Hom. pool) 6.84


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 7102664
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.29|P-value:5.03E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:-4.35|P-value:6.86E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC23(YHR166C)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ERG25(YGR060W)|FD-Score:3.39|P-value:3.56E-4|Clearance:0.31||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:NOP56(YLR197W)|FD-Score:-4.56|P-value:2.58E-6|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUG1(YER006W)|FD-Score:5|P-value:2.88E-7|Clearance:0.26||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PCF11(YDR228C)|FD-Score:-3.48|P-value:2.47E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POP1(YNL221C)|FD-Score:-3.13|P-value:8.74E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP3(YDR473C)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAT1(YOR048C)|FD-Score:-3.25|P-value:5.77E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RER2(YBR002C)|FD-Score:-3.84|P-value:6.05E-5|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC1(YOR217W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPC10(YHR143W-A)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL25(YOL127W)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPP1(YHR062C)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSM10(YDR041W)|FD-Score:-3.38|P-value:3.65E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SEC31(YDL195W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPT14(YPL175W)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.41||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SPT15(YER148W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUI2(YJR007W)|FD-Score:-5.4|P-value:3.37E-8|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF12(YDR145W)|FD-Score:-4.8|P-value:8.03E-7|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TSR1(YDL060W)|FD-Score:4.17|P-value:1.52E-5|Clearance:0.32||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YTH1(YPR107C)|FD-Score:5.55|P-value:1.46E-8|Clearance:0.55||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:CCT3(YJL014W)|FD-Score:-3.29|P-value:5.03E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:-4.35|P-value:6.86E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC23(YHR166C)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ERG25(YGR060W)|FD-Score:3.39|P-value:3.56E-4|Clearance:0.31||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:NOP56(YLR197W)|FD-Score:-4.56|P-value:2.58E-6|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUG1(YER006W)|FD-Score:5|P-value:2.88E-7|Clearance:0.26||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PCF11(YDR228C)|FD-Score:-3.48|P-value:2.47E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POP1(YNL221C)|FD-Score:-3.13|P-value:8.74E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP3(YDR473C)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAT1(YOR048C)|FD-Score:-3.25|P-value:5.77E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RER2(YBR002C)|FD-Score:-3.84|P-value:6.05E-5|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC1(YOR217W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPC10(YHR143W-A)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL25(YOL127W)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPP1(YHR062C)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSM10(YDR041W)|FD-Score:-3.38|P-value:3.65E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SEC31(YDL195W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPT14(YPL175W)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.41||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SPT15(YER148W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUI2(YJR007W)|FD-Score:-5.4|P-value:3.37E-8|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF12(YDR145W)|FD-Score:-4.8|P-value:8.03E-7|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TSR1(YDL060W)|FD-Score:4.17|P-value:1.52E-5|Clearance:0.32||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YTH1(YPR107C)|FD-Score:5.55|P-value:1.46E-8|Clearance:0.55||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 7102664
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:APD1(YBR151W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ATP15(YPL271W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD17(YNR027W)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CAF20(YOR276W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CDC26(YFR036W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CSM4(YPL200W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DSS4(YPR017C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ERV41(YML067C)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FMP37(YGL080W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GCN5(YGR252W)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GYP1(YOR070C)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOT1(YMR172W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:HTA2(YBL003C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IES1(YFL013C)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC25(YLR021W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:ISF1(YMR081C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LEM3(YNL323W)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LHS1(YKL073W)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MDY2(YOL111C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF2(YJL102W)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL28(YDR462W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL38(YKL170W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL7(YDR237W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS35(YGR165W)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSH3(YCR092C)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MZM1(YDR493W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NRP1(YDL167C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:OSH7(YHR001W)|FD-Score:3.72|P-value:9.81E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PET123(YOR158W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PIF1(YML061C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKH1(YDR490C)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PMR1(YGL167C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PTK2(YJR059W)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RPL17B(YJL177W)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:-4.6|P-value:2.14E-6||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC6(YDR129C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SCY1(YGL083W)|FD-Score:4.31|P-value:8.05E-6||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SDT1(YGL224C)|FD-Score:4.83|P-value:6.93E-7||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SEC22(YLR268W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFB3(YHR098C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGM1(YJR134C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SRB2(YHR041C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SSD1(YDR293C)|FD-Score:-5.44|P-value:2.71E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN8(YNL025C)|FD-Score:7.51|P-value:2.88E-14||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STR2(YJR130C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:SYG1(YIL047C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TCO89(YPL180W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TRP3(YKL211C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBC5(YDR059C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UGA3(YDL170W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS21(YOR089C)|FD-Score:4.87|P-value:5.49E-7||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR285W(YBR285W_p)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YDR094W(YDR094W_d)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YGR025W(YGR025W_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJR115W(YJR115W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YMR082C(YMR082C_d)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL162W(YOL162W_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR385W(YOR385W_p)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPR003C(YPR003C_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPT35(YHR105W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:AAC1(YMR056C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:APD1(YBR151W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ATP15(YPL271W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD17(YNR027W)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CAF20(YOR276W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CDC26(YFR036W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CSM4(YPL200W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DSS4(YPR017C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ERV41(YML067C)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FMP37(YGL080W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GCN5(YGR252W)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GYP1(YOR070C)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOT1(YMR172W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:HTA2(YBL003C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IES1(YFL013C)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC25(YLR021W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:ISF1(YMR081C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LEM3(YNL323W)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LHS1(YKL073W)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MDY2(YOL111C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF2(YJL102W)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL28(YDR462W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL38(YKL170W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL7(YDR237W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS35(YGR165W)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSH3(YCR092C)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MZM1(YDR493W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NRP1(YDL167C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:OSH7(YHR001W)|FD-Score:3.72|P-value:9.81E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PET123(YOR158W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PIF1(YML061C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKH1(YDR490C)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PMR1(YGL167C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PTK2(YJR059W)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RPL17B(YJL177W)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:-4.6|P-value:2.14E-6||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC6(YDR129C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SCY1(YGL083W)|FD-Score:4.31|P-value:8.05E-6||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SDT1(YGL224C)|FD-Score:4.83|P-value:6.93E-7||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SEC22(YLR268W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFB3(YHR098C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGM1(YJR134C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SRB2(YHR041C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SSD1(YDR293C)|FD-Score:-5.44|P-value:2.71E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN8(YNL025C)|FD-Score:7.51|P-value:2.88E-14||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STR2(YJR130C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:SYG1(YIL047C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TCO89(YPL180W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TRP3(YKL211C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBC5(YDR059C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UGA3(YDL170W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS21(YOR089C)|FD-Score:4.87|P-value:5.49E-7||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR285W(YBR285W_p)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YDR094W(YDR094W_d)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YGR025W(YGR025W_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJR115W(YJR115W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YMR082C(YMR082C_d)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL162W(YOL162W_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR385W(YOR385W_p)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPR003C(YPR003C_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPT35(YHR105W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C5.551.46E-80.55YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YER006W5.002.88E-70.26NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YOL127W4.741.08E-60.06RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YHR166C4.671.48E-60.50CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YDL060W4.171.52E-50.32TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YPL175W3.855.97E-50.41SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YDL195W3.442.89E-40.04SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YOR217W3.403.33E-40.02RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YGR060W3.393.56E-40.31ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YKL141W3.070.001060.17SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YFR050C2.900.001880.04PRE4Beta 7 subunit of the 20S proteasome
YMR314W2.860.002100.06PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YNL113W2.800.002570.01RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YOL139C2.790.002660.02CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YKL095W2.760.002860.06YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL025C7.512.88E-14SSN8Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance
YCR092C5.581.18E-8MSH3Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
YML067C4.993.00E-7ERV41Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
YOR089C4.875.49E-7VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YGL224C4.836.93E-7SDT1Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives
YDR490C4.827.02E-7PKH1Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p
YOR070C4.827.25E-7GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YBR151W4.701.29E-6APD1Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
YOR276W4.681.43E-6CAF20Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E
YJR130C4.661.56E-6STR2Cystathionine gamma-synthase, converts cysteine into cystathionine
YGL083W4.318.05E-6SCY1Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
YFL013C4.298.98E-6IES1Subunit of the INO80 chromatin remodeling complex
YMR082C_d4.261.02E-5YMR082C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR017C4.032.78E-5DSS4Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
YDR094W_d4.022.85E-5YDR094W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2

GO enrichment analysis for SGTC_1330
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1157.89E-19SGTC_444curcumin 90.4 μMICCB bioactive library66103320.164706iron homeostasis
0.1126.43E-18SGTC_1780st038448 24.5 μMTimTec (Natural product derivative library)7288680.120482iron homeostasis
0.1071.93E-16SGTC_3740880-0231 82.3 μMChemDiv (Drug-like library)68335870.247059iron homeostasis
0.1062.56E-16SGTC_525k781-2675 26.7 μMChemDiv (Drug-like library)36511180.155844redox potentiating
0.1048.35E-16SGTC_2675theaflavin digallate 100.0 μMMicrosource (Natural product library)4673200.0857143iron homeostasis
0.0961.61E-13SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004550.101124
0.0953.59E-13SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.283951
0.0939.93E-13SGTC_3393091-4833 71.4 μMChemDiv (Drug-like library)32400770.1375iron homeostasis
0.0897.12E-12SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.0769231
0.0871.91E-11SGTC_1658st012944 20.0 μMTimTec (Natural product derivative library)242072600.0705882iron homeostasis
0.0855.02E-11SGTC_2803448-3607 3.0 μMChemDiv (Drug-like library)6261790.088888960S ribosome export
0.0841.08E-10SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.0963855fatty acid desaturase (OLE1)
0.0822.49E-10SGTC_22757947463 200.0 μMChembridge (Fragment library)12254140.166667iron homeostasis
0.0806.13E-10SGTC_14063909-7906 11.9 μMChemDiv (Drug-like library)X14060.10344860S ribosome export
0.0808.74E-10SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.112245ERAD & cell cycle

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5720960-008717.7 μM0.4675326796365ChemDiv (Drug-like library)444.869784.8426
SGTC_10513253-390378 μM0.4027785509239ChemDiv (Drug-like library)438.105445.44223
SGTC_9781416-051287.4 μM0.3970596814003ChemDiv (Drug-like library)270.283260.69424redox potentiating
SGTC_30833-058795.58 μM0.3650795382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_10523253-3998198 μM0.3589745450317ChemDiv (Drug-like library)370.359322.71127
SGTC_8550428-002025 μM0.338716812414ChemDiv (Drug-like library)226.23072.15914Golgi
SGTC_4271354-005347.3 μM0.3378386813657ChemDiv (Drug-like library)407.892684.19625
SGTC_6604031-1742102 μM0.3376626738706ChemDiv (Drug-like library)300.335680.18925redox potentiating
SGTC_12082323-234750.3 μM0.3333335412760ChemDiv (Drug-like library)346.405664.22724
SGTC_12092323-234750.3 μM0.3333335412760ChemDiv (Drug-like library)346.405664.22724
SGTC_1731st03596463 μM0.3333336739575TimTec (Natural product derivative library)317.253640.26547