1486-0610

7-heptyl-3-methyl-8-propan-2-ylsulfanylpurine-2,6-dione

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1334
Screen concentration 236.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3742339
SMILES CCCCCCCN1C2=C(N=C1SC(C)C)N(C(=O)NC2=O)C
Standardized SMILES CCCCCCCn1c(SC(C)C)nc2N(C)C(=O)NC(=O)c12
Molecular weight 338.4682
ALogP 4.2
H-bond donor count 1
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.04
% growth inhibition (Hom. pool) 5.99


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3742339
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:3.79|P-value:7.40E-5|Clearance:0.32||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:DBF4(YDR052C)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.05||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DML1(YMR211W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.32||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:EMG1(YLR186W)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GNA1(YFL017C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.03||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NAF1(YNL124W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.02||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NMD3(YHR170W)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.32||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PKC1(YBL105C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.08||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RFA1(YAR007C)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RFC5(YBR087W)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.32||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPN7(YPR108W)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPP1(YHR062C)|FD-Score:3.89|P-value:5.04E-5|Clearance:0.32||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RSC4(YKR008W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SAR1(YPL218W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.32||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:UBC1(YDR177W)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.32||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDL221W(YDL221W_d)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:BMS1(YPL217C)|FD-Score:3.79|P-value:7.40E-5|Clearance:0.32||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:DBF4(YDR052C)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.05||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DML1(YMR211W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.32||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:EMG1(YLR186W)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GNA1(YFL017C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.03||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NAF1(YNL124W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.02||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NMD3(YHR170W)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.32||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PKC1(YBL105C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.08||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RFA1(YAR007C)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RFC5(YBR087W)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.32||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPN7(YPR108W)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPP1(YHR062C)|FD-Score:3.89|P-value:5.04E-5|Clearance:0.32||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RSC4(YKR008W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:SAR1(YPL218W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.32||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:UBC1(YDR177W)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.32||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDL221W(YDL221W_d)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3742339
Download HOP data (tab-delimited text)  (excel)
Gene:ABP140(YOR239W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:APL5(YPL195W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARF1(YDL192W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP8(YOR141C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:BNS1(YGR230W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis Gene:CHS3(YBR023C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS6(YJL099W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:4.78|P-value:8.66E-7||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CNM67(YNL225C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CTF4(YPR135W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DRS2(YAL026C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:FAB1(YFR019W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:GCG1(YER163C_p)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GDA1(YEL042W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GIS2(YNL255C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:IDH2(YOR136W)|FD-Score:6.28|P-value:1.65E-10||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IWR1(YDL115C)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JJJ3(YJR097W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LAS21(YJL062W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MRPL23(YOR150W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB4(YOL112W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NPR3(YHL023C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NUP53(YMR153W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:ORM1(YGR038W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:RAM1(YDL090C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:SNQ2(YDR011W)|FD-Score:4.33|P-value:7.52E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUR4(YLR372W)|FD-Score:6.64|P-value:1.62E-11||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYG1(YIL047C)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:SYS1(YJL004C)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TSR3(YOR006C)|FD-Score:5.22|P-value:9.15E-8||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:UFD2(YDL190C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:URA5(YML106W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR300C(YBR300C_d)|FD-Score:3.78|P-value:7.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Putative protein of unknown function Gene:YDL032W(YDL032W_d)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL085C-A(YDL085C-A_p)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YHL044W(YHL044W_p)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIP4(YGL198W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL120W(YJL120W_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR039W(YJR039W_p)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR033C(YKR033C_d)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLR363W-A(YLR363W-A_p)|FD-Score:4.78|P-value:8.67E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YLR419W(YLR419W_p)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL179C(YNL179C_d)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR052C(YOR052C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR072W(YOR072W_d)|FD-Score:6.08|P-value:6.15E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR385W(YOR385W_p)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR012W(YPR012W_d)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR148C(YPR148C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ABP140(YOR239W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:APL5(YPL195W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARF1(YDL192W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP8(YOR141C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:BNS1(YGR230W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis Gene:CHS3(YBR023C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS6(YJL099W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:4.78|P-value:8.66E-7||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CNM67(YNL225C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CTF4(YPR135W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DRS2(YAL026C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:FAB1(YFR019W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:GCG1(YER163C_p)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GDA1(YEL042W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GIS2(YNL255C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:IDH2(YOR136W)|FD-Score:6.28|P-value:1.65E-10||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IWR1(YDL115C)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JJJ3(YJR097W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LAS21(YJL062W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MRPL23(YOR150W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB4(YOL112W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NPR3(YHL023C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NUP53(YMR153W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:ORM1(YGR038W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:RAM1(YDL090C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:SNQ2(YDR011W)|FD-Score:4.33|P-value:7.52E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUR4(YLR372W)|FD-Score:6.64|P-value:1.62E-11||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYG1(YIL047C)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:SYS1(YJL004C)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TSR3(YOR006C)|FD-Score:5.22|P-value:9.15E-8||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:UFD2(YDL190C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:URA5(YML106W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR300C(YBR300C_d)|FD-Score:3.78|P-value:7.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Putative protein of unknown function Gene:YDL032W(YDL032W_d)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL085C-A(YDL085C-A_p)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YHL044W(YHL044W_p)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIP4(YGL198W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL120W(YJL120W_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR039W(YJR039W_p)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR033C(YKR033C_d)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLR363W-A(YLR363W-A_p)|FD-Score:4.78|P-value:8.67E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YLR419W(YLR419W_p)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL179C(YNL179C_d)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR052C(YOR052C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR072W(YOR072W_d)|FD-Score:6.08|P-value:6.15E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR385W(YOR385W_p)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR012W(YPR012W_d)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR148C(YPR148C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL221W_d3.904.77E-50.32YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YHR062C3.895.04E-50.32RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR170W3.807.27E-50.32NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YPL217C3.797.40E-50.32BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YDR177W3.671.24E-40.32UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YMR211W3.661.24E-40.32DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YBR087W3.581.69E-40.32RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YPL218W3.561.86E-40.32SAR1GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport
YDR052C3.245.94E-40.05DBF4Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress
YFL017C3.206.98E-40.03GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YNL124W3.167.85E-40.02NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YBL105C3.148.40E-40.08PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YNL062C3.070.001080.09GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YBR060C2.980.001440.01ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YMR001C2.970.001500.01CDC5Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR372W6.641.62E-11SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YOR136W6.281.65E-10IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
YOR072W_d6.086.15E-10YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YOR006C5.229.15E-8TSR3Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YNL298W4.788.66E-7CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YLR363W-A_p4.788.67E-7YLR363W-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
YDL115C4.434.67E-6IWR1RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
YOR385W_p4.347.09E-6YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YDR011W4.337.52E-6SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YDL192W4.241.10E-5ARF1ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
YCR100C_p4.191.37E-5YCR100C_pPutative protein of unknown function
YOR052C4.171.50E-5YOR052CNuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress
YDL090C4.171.52E-5RAM1Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
YDL085C-A_p4.052.51E-5YDL085C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YNL255C4.042.62E-5GIS2Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2

GO enrichment analysis for SGTC_1334
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1262.15E-22SGTC_1663st014140 55.4 μMTimTec (Natural product derivative library)42071200.033333360S ribosome export
0.1211.29E-20SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.062560S ribosome export
0.1111.59E-17SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0987654RPP1 & pyrimidine depletion
0.1001.62E-14SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.035294160S ribosome export
0.0921.33E-12SGTC_5941487-1584 10.8 μMChemDiv (Drug-like library)50133320.047619
0.0921.39E-12SGTC_2740felodipine 17.1 μMMiscellaneous33330.0875
0.0905.52E-12SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.07608760S ribosome export
0.0897.36E-12SGTC_1664st014217 9.0 μMTimTec (Natural product derivative library)39827820.032608760S ribosome export
0.0881.07E-11SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.0266667
0.0858.36E-11SGTC_20422-0095 29.0 μMChemDiv (Drug-like library)67908400.027027redox potentiating
0.0831.63E-10SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.0789474
0.0815.48E-10SGTC_10613448-9325 110.0 μMChemDiv (Drug-like library)7234820.0649351
0.0791.22E-9SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0574713RPP1 & pyrimidine depletion
0.0791.45E-9SGTC_13251431-0015 227.0 μMChemDiv (Drug-like library)11033400.028169Golgi
0.0765.10E-9SGTC_1360097-0015 404.5 μMChemDiv (Drug-like library)48613370.028571460S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1899st06325459.6 μM0.4736843093813TimTec (Natural product derivative library)335.444463.96714
SGTC_1676st01848923.4 μM0.3333333696235TimTec (Natural product derivative library)306.403243.5090360S ribosome export
SGTC_1675st01848524.5 μM0.281253323024TimTec (Natural product derivative library)306.403243.5090360S ribosome export
SGTC_1736st03677451 μM0.2727271272247TimTec (Natural product derivative library)392.47414.17904
SGTC_1074carmofur3.8 μM0.2615382577NIH Clinical Collection257.2614431.12524exosome
SGTC_2348902254333.42 μM0.2539683162678Chembridge (Fragment library)275.306380.8303mitochondrial processes
SGTC_23759072683200 μM0.24615416798029Chembridge (Fragment library)261.27980.54703mitochondrial processes
SGTC_1740st03828613.22 μM0.230769716786TimTec (Natural product derivative library)266.319461.29213
SGTC_12760871-006894.2 μM0.2241383414906ChemDiv (Drug-like library)261.319562.57104
SGTC_1791st04831356.1 μM0.2133331204976TimTec (Natural product derivative library)356.37582.86505