1486-1464

3-[(4-methylphenyl)carbamoyl]-1-methylsulfonyl-1-pentylurea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1342
Screen concentration 234.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3253938
SMILES CCCCCN(C(=O)NC(=O)NC1=CC=C(C=C1)C)S(=O)(=O)C
Standardized SMILES CCCCCN(C(=O)NC(=O)Nc1ccc(C)cc1)S(=O)(=O)C
Molecular weight 341.4258
ALogP 2.78
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 30.62
% growth inhibition (Hom. pool) 4.19


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3253938
Download HIP data (tab-delimited text)  (excel)
Gene:CTR86(YCR054C)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:CWC2(YDL209C)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.88||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:KAR1(YNL188W)|FD-Score:3.15|P-value:8.12E-4|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NOG1(YPL093W)|FD-Score:3.17|P-value:7.66E-4|Clearance:0.02||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PRP2(YNR011C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RPB11(YOL005C)|FD-Score:4.75|P-value:9.95E-7|Clearance:0.88||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RRP42(YDL111C)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.14||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RSC9(YML127W)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.3||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEN15(YMR059W)|FD-Score:-3.12|P-value:8.97E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:STN1(YDR082W)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TAM41(YGR046W)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.04||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TIF6(YPR016C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.02||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YCS4(YLR272C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL152W(YDL152W_d)|FD-Score:3.22|P-value:6.45E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:CTR86(YCR054C)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:CWC2(YDL209C)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.88||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:KAR1(YNL188W)|FD-Score:3.15|P-value:8.12E-4|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NOG1(YPL093W)|FD-Score:3.17|P-value:7.66E-4|Clearance:0.02||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PRP2(YNR011C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RPB11(YOL005C)|FD-Score:4.75|P-value:9.95E-7|Clearance:0.88||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RRP42(YDL111C)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.14||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RSC9(YML127W)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.3||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEN15(YMR059W)|FD-Score:-3.12|P-value:8.97E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:STN1(YDR082W)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TAM41(YGR046W)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.04||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TIF6(YPR016C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.02||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YCS4(YLR272C)|FD-Score:-3.32|P-value:4.44E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL152W(YDL152W_d)|FD-Score:3.22|P-value:6.45E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3253938
Download HOP data (tab-delimited text)  (excel)
Gene:AIM43(YPL099C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APM3(YBR288C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ASE1(YOR058C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATG15(YCR068W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG16(YMR159C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP3(YBR039W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCK2(YER167W)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BSC6(YOL137W)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BUD19(YJL188C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CDC26(YFR036W)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLC1(YGR167W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG5(YNL051W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSH1(YBR161W)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DDR2(YOL052C-A)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:EAF7(YNL136W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECM25(YJL201W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:EST2(YLR318W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:HAT1(YPL001W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HOM6(YJR139C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IME2(YJL106W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:MDE1(YJR024C)|FD-Score:-4.45|P-value:4.34E-6||SGD DESC:5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant Gene:MDH2(YOL126C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MLS1(YNL117W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:NAS6(YGR232W)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:NPR3(YHL023C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:OPI11(YPR044C_d)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:OTU2(YHL013C)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PCL9(YDL179W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PGD1(YGL025C)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIG2(YIL045W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:RBL2(YOR265W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RPS1B(YML063W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP1(YER047C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SBP1(YHL034C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SLF1(YDR515W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLP1(YOR154W_p)|FD-Score:-4.57|P-value:2.39E-6||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLX1(YBR228W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SSD1(YDR293C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUL1(YBR294W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TGL2(YDR058C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TSC3(YBR058C-A)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP3(YER151C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS1(YKR001C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS30(YPL120W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YBR196C-B(YBR196C-B_p)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDL071C(YDL071C_d)|FD-Score:4|P-value:3.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR344C(YDR344C_d)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGL042C(YGL042C_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGL072C(YGL072C_d)|FD-Score:5.28|P-value:6.42E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL176C(YGL176C_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR066C(YGR066C_p)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Putative protein of unknown function Gene:YKR070W(YKR070W_p)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL047W(YLL047W_d)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML037C(YML037C_p)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YML094C-A(YML094C-A_d)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR226C(YMR226C)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YPL035C(YPL035C_d)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPL197C(YPL197C_d)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:AIM43(YPL099C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APM3(YBR288C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ASE1(YOR058C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATG15(YCR068W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG16(YMR159C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP3(YBR039W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCK2(YER167W)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BSC6(YOL137W)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BUD19(YJL188C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CDC26(YFR036W)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLC1(YGR167W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG5(YNL051W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSH1(YBR161W)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DDR2(YOL052C-A)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:EAF7(YNL136W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECM25(YJL201W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:EST2(YLR318W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:HAT1(YPL001W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HOM6(YJR139C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IME2(YJL106W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:MDE1(YJR024C)|FD-Score:-4.45|P-value:4.34E-6||SGD DESC:5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant Gene:MDH2(YOL126C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MLS1(YNL117W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:NAS6(YGR232W)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:NPR3(YHL023C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:OPI11(YPR044C_d)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:OTU2(YHL013C)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PCL9(YDL179W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PGD1(YGL025C)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIG2(YIL045W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:RBL2(YOR265W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RPS1B(YML063W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP1(YER047C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SBP1(YHL034C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SLF1(YDR515W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SLP1(YOR154W_p)|FD-Score:-4.57|P-value:2.39E-6||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SLX1(YBR228W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SSD1(YDR293C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUL1(YBR294W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TGL2(YDR058C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TSC3(YBR058C-A)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP3(YER151C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS1(YKR001C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS30(YPL120W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YBR196C-B(YBR196C-B_p)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDL071C(YDL071C_d)|FD-Score:4|P-value:3.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR344C(YDR344C_d)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGL042C(YGL042C_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGL072C(YGL072C_d)|FD-Score:5.28|P-value:6.42E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL176C(YGL176C_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR066C(YGR066C_p)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Putative protein of unknown function Gene:YKR070W(YKR070W_p)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL047W(YLL047W_d)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML037C(YML037C_p)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YML094C-A(YML094C-A_d)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR226C(YMR226C)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YPL035C(YPL035C_d)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPL197C(YPL197C_d)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL005C4.759.95E-70.88RPB11RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
YDL209C4.434.72E-60.88CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YNR011C3.551.90E-40.02PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YPR016C3.542.02E-40.02TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YML127W3.522.16E-40.30RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDL152W_d3.226.45E-40.05YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YPL093W3.177.66E-40.02NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YNL188W3.158.12E-40.00KAR1Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
YGR046W3.158.19E-40.04TAM41Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
YDL111C3.119.27E-40.14RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YGL093W2.970.001500.02SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YPR133C2.940.001620.03SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YGL247W2.920.001770.02BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YGR114C_d2.900.001860.00YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YGR013W2.900.001880.05SNU71Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL072C_d5.286.42E-8YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YOL052C-A5.201.02E-7DDR2Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
YMR226C4.934.02E-7YMR226CNADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments
YER167W4.875.47E-7BCK2Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations
YMR159C4.592.22E-6ATG16Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YOL137W4.513.31E-6BSC6Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
YGL025C4.395.70E-6PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YER151C4.376.09E-6UBP3Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
YGR066C_p4.337.43E-6YGR066C_pPutative protein of unknown function
YLR318W4.251.07E-5EST2Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia
YBR228W4.181.48E-5SLX1Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
YFR036W4.121.89E-5CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YML063W4.042.65E-5RPS1BRibosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication
YDL071C_d4.003.16E-5YDL071C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W
YDL204W4.003.21E-5RTN2Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1342
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1231.79E-21SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.113924TSC3-RPN4
0.1194.42E-20SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0615385TSC3-RPN4
0.1119.22E-18SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0289855TSC3-RPN4
0.1001.73E-14SGTC_488nitrendipine 139.0 μMMiscellaneous45070.12987
0.0983.88E-14SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0597015TSC3-RPN4
0.0921.16E-12SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0895522TSC3-RPN4
0.0921.78E-12SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.109589TSC3-RPN4
0.0913.23E-12SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0923077
0.0904.52E-12SGTC_31219125696 49.5 μMChembridge (Drug-like library)12353650.05
0.0897.32E-12SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.104478TSC3-RPN4
0.0871.95E-11SGTC_12390337-0584 35.4 μMChemDiv (Drug-like library)9143010.09375unfolded protein response
0.0872.38E-11SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.126582PDR1
0.0872.41E-11SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.175TSC3-RPN4
0.0863.12E-11SGTC_2775cyproheptadine 69.6 μMMiscellaneous29130.0634921
0.0841.11E-10SGTC_1711st032263 62.8 μMTimTec (Natural product derivative library)39589790.0617284

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3411486-1430230 μM0.739132054753ChemDiv (Drug-like library)347.817722.524
SGTC_13361486-0901104 μM0.625006114ChemDiv (Drug-like library)389.468623.86824
SGTC_13411486-1360280 μM0.63976547ChemDiv (Drug-like library)285.31950.99324
SGTC_7391486-1183203 μM0.5614044346692ChemDiv (Drug-like library)437.940265.01924
SGTC_2851486-132546 μM0.44642922310230ChemDiv (Drug-like library)457.92995.29624
SGTC_13401486-1359257 μM0.4339624309068ChemDiv (Drug-like library)285.31950.99324
SGTC_13351486-0900202 μM0.42623X1335ChemDiv (Drug-like library)395.516254.1542
SGTC_13371486-101140.3 μM0.423729X1337ChemDiv (Drug-like library)443.903314.8142
SGTC_11901486-133213 μM0.4218754150046ChemDiv (Drug-like library)487.955885.14325
SGTC_13391486-132122.7 μM0.43469382ChemDiv (Drug-like library)443.903324.8124ERG2