1488-2224

2,3-diphenyl-N-(1,2,2,2-tetrachloroethyl)-1,2,4-thiadiazol-2-ium-5-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1346
Screen concentration 62.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 3722427
SMILES C1=CC=C(C=C1)C2=[N+](SC(=N2)NC(C(Cl)(Cl)Cl)Cl)C3=CC=CC=C3
Standardized SMILES ClC(Nc1nc(c2ccccc2)[n+](s1)c3ccccc3)C(Cl)(Cl)Cl
Molecular weight 420.1636
ALogP 6.63
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 35.87
% growth inhibition (Hom. pool) 7.12


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3722427
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:5.72|P-value:5.24E-9|Clearance:0.6||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.01||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.09||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:4.61|P-value:2.04E-6|Clearance:0.21||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:ERG11(YHR007C)|FD-Score:4.29|P-value:8.74E-6|Clearance:0.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HEM1(YDR232W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.01||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:ILS1(YBL076C)|FD-Score:3.29|P-value:5.04E-4|Clearance:0.04||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:ISD11(YER048W-A)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:NET1(YJL076W)|FD-Score:4.06|P-value:2.45E-5|Clearance:0.02||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:PAB1(YER165W)|FD-Score:3.84|P-value:6.07E-5|Clearance:0.04||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PKC1(YBL105C)|FD-Score:-4.91|P-value:4.64E-7|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE5(YMR314W)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.07||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP24(YMR268C)|FD-Score:3.25|P-value:5.86E-4|Clearance:0.06||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RER2(YBR002C)|FD-Score:5.94|P-value:1.44E-9|Clearance:0.6||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RRP43(YCR035C)|FD-Score:4.27|P-value:9.67E-6|Clearance:0.13||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:4.4|P-value:5.51E-6|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.13||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SPB4(YFL002C)|FD-Score:3.8|P-value:7.12E-5|Clearance:0.12||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC98(YNL126W)|FD-Score:4.14|P-value:1.70E-5|Clearance:0.08||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SSU72(YNL222W)|FD-Score:5.2|P-value:9.75E-8|Clearance:0.6||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:TFA1(YKL028W)|FD-Score:6.42|P-value:6.90E-11|Clearance:0.6||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF35(YDR429C)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP11(YKL099C)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.15||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP25(YIL091C)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YDR526C(YDR526C_d)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL153W(YKL153W_d)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:ACC1(YNR016C)|FD-Score:5.72|P-value:5.24E-9|Clearance:0.6||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.01||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.09||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:4.61|P-value:2.04E-6|Clearance:0.21||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:ERG11(YHR007C)|FD-Score:4.29|P-value:8.74E-6|Clearance:0.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HEM1(YDR232W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.01||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:ILS1(YBL076C)|FD-Score:3.29|P-value:5.04E-4|Clearance:0.04||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:ISD11(YER048W-A)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:NET1(YJL076W)|FD-Score:4.06|P-value:2.45E-5|Clearance:0.02||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:PAB1(YER165W)|FD-Score:3.84|P-value:6.07E-5|Clearance:0.04||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PKC1(YBL105C)|FD-Score:-4.91|P-value:4.64E-7|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE5(YMR314W)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.07||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP24(YMR268C)|FD-Score:3.25|P-value:5.86E-4|Clearance:0.06||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RER2(YBR002C)|FD-Score:5.94|P-value:1.44E-9|Clearance:0.6||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RRP43(YCR035C)|FD-Score:4.27|P-value:9.67E-6|Clearance:0.13||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:4.4|P-value:5.51E-6|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.13||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SPB4(YFL002C)|FD-Score:3.8|P-value:7.12E-5|Clearance:0.12||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC98(YNL126W)|FD-Score:4.14|P-value:1.70E-5|Clearance:0.08||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SSU72(YNL222W)|FD-Score:5.2|P-value:9.75E-8|Clearance:0.6||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:TFA1(YKL028W)|FD-Score:6.42|P-value:6.90E-11|Clearance:0.6||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF35(YDR429C)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP11(YKL099C)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.15||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP25(YIL091C)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YDR526C(YDR526C_d)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL153W(YKL153W_d)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3722427
Download HOP data (tab-delimited text)  (excel)
Gene:ADK2(YER170W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM39(YOL053W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:7.87|P-value:1.82E-15||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:5.75|P-value:4.58E-9||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:BAP2(YBR068C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BIK1(YCL029C)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:ECM29(YHL030W)|FD-Score:5.03|P-value:2.41E-7||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ECM7(YLR443W)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:GCS1(YDL226C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HAL9(YOL089C)|FD-Score:5.1|P-value:1.67E-7||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HOP2(YGL033W)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IMP2'(YIL154C)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MRPL38(YKL170W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MTG1(YMR097C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NBP2(YDR162C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PAL1(YDR348C)|FD-Score:7.37|P-value:8.67E-14||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PCL10(YGL134W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PDB1(YBR221C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:RPS21B(YJL136C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:SCT1(YBL011W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SHE4(YOR035C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SKM1(YOL113W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SWF1(YDR126W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THR1(YHR025W)|FD-Score:7.27|P-value:1.85E-13||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:4.94|P-value:3.84E-7||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TMA19(YKL056C)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TOP1(YOL006C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TPO4(YOR273C)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP3(YKL211C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:7.2|P-value:2.98E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-6.27|P-value:1.77E-10||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TYE7(YOR344C)|FD-Score:5.24|P-value:8.09E-8||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UBX2(YML013W)|FD-Score:6.14|P-value:4.18E-10||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGA3(YDL170W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:URE2(YNL229C)|FD-Score:12|P-value:2.06E-33||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VBA2(YBR293W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS25(YJR102C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBR012C(YBR012C_d)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR056W(YBR056W_p)|FD-Score:4.53|P-value:2.96E-6||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDL026W(YDL026W_d)|FD-Score:5.83|P-value:2.70E-9||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR061W(YDR061W_p)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR282C(YDR282C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YFL015C(YFL015C_d)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YIR007W(YIR007W_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YJL160C(YJL160C_p)|FD-Score:-4.71|P-value:1.26E-6||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR142W(YJR142W_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YLR444C(YLR444C_d)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR027W(YMR027W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL034W(YPL034W_p)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:ADK2(YER170W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM39(YOL053W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:7.87|P-value:1.82E-15||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:5.75|P-value:4.58E-9||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:BAP2(YBR068C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BIK1(YCL029C)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:ECM29(YHL030W)|FD-Score:5.03|P-value:2.41E-7||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ECM7(YLR443W)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:GCS1(YDL226C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HAL9(YOL089C)|FD-Score:5.1|P-value:1.67E-7||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HOP2(YGL033W)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IMP2'(YIL154C)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MRPL38(YKL170W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MTG1(YMR097C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NBP2(YDR162C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PAL1(YDR348C)|FD-Score:7.37|P-value:8.67E-14||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PCL10(YGL134W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PDB1(YBR221C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:RPS21B(YJL136C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:SCT1(YBL011W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SHE4(YOR035C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SKM1(YOL113W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SWF1(YDR126W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THR1(YHR025W)|FD-Score:7.27|P-value:1.85E-13||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:4.94|P-value:3.84E-7||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TMA19(YKL056C)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TOP1(YOL006C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TPO4(YOR273C)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP3(YKL211C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:7.2|P-value:2.98E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-6.27|P-value:1.77E-10||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TYE7(YOR344C)|FD-Score:5.24|P-value:8.09E-8||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UBX2(YML013W)|FD-Score:6.14|P-value:4.18E-10||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGA3(YDL170W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:URE2(YNL229C)|FD-Score:12|P-value:2.06E-33||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VBA2(YBR293W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS25(YJR102C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBR012C(YBR012C_d)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR056W(YBR056W_p)|FD-Score:4.53|P-value:2.96E-6||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDL026W(YDL026W_d)|FD-Score:5.83|P-value:2.70E-9||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR061W(YDR061W_p)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR282C(YDR282C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YFL015C(YFL015C_d)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YIR007W(YIR007W_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YJL160C(YJL160C_p)|FD-Score:-4.71|P-value:1.26E-6||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR142W(YJR142W_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YLR444C(YLR444C_d)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR027W(YMR027W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL034W(YPL034W_p)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL028W6.426.90E-110.60TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YBR002C5.941.44E-90.60RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YNR016C5.725.24E-90.60ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YNL222W5.209.75E-80.60SSU72Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo
YHR036W4.612.04E-60.21BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YLR033W4.405.51E-60.10RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YHR007C4.298.74E-60.02ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YCR035C4.279.67E-60.13RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YNL126W4.141.70E-50.08SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YJL076W4.062.45E-50.02NET1Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
YMR314W4.042.63E-50.07PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YML127W3.973.59E-50.13RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YER165W3.846.07E-50.04PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YFL002C3.807.12E-50.12SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YKL153W_d3.681.15E-40.04YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL229C12.002.06E-33URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YDR127W7.871.82E-15ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR348C7.378.67E-14PAL1Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
YHR025W7.271.85E-13THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YBR058C-A7.202.98E-13TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML013W6.144.18E-10UBX2Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
YDL026W_d5.832.70E-9YDL026W_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YPR060C5.754.58E-9ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YOR344C5.248.09E-8TYE7Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression
YKL211C5.171.16E-7TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR126W5.131.43E-7SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YOL089C5.101.67E-7HAL9Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
YHL030W5.032.41E-7ECM29Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
YCR053W4.943.84E-7THR4Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
YFL015C_d4.681.45E-6YFL015C_dDubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene

GO enrichment analysis for SGTC_1346
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2831.20E-108SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.106061SWF1 & branched chain AA biosynthesis
0.2443.09E-80SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.129032
0.2323.02E-72SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.142857
0.2281.93E-70SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.084507
0.2131.56E-61SGTC_590330-0160 1.3 μMChemDiv (Drug-like library)42934820.123288
0.2053.50E-57SGTC_1726st035556 14.6 μMTimTec (Natural product derivative library)59194390.0967742
0.2005.30E-54SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0461538
0.1971.45E-52SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.118644TSC3-RPN4
0.1963.17E-52SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.101449RSC complex & mRNA processing
0.1929.55E-50SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0606061TSC3-RPN4
0.1869.84E-47SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.0958904
0.1843.30E-46SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.030303TSC3-RPN4
0.1831.75E-45SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0925926
0.1822.72E-45SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.115942TSC3-RPN4
0.1791.12E-43SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0217391

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_11951502-014741.1 μM0.510638817940ChemDiv (Drug-like library)282.383344.86613
SGTC_9841492-02552.91 μM0.3396231585198ChemDiv (Drug-like library)344.452726.12813SWF1 & branched chain AA biosynthesis
SGTC_13501495-0454146 μM0.2333331069057ChemDiv (Drug-like library)350.843043.92615
SGTC_1524469-0003551.23 μM0.232143697280ChemDiv (Drug-like library)268.333584.08224
SGTC_6281636-025316 μM0.2321434456143ChemDiv (Drug-like library)331.230245.07213sphingolipid biosynthesis & PDR1
SGTC_8800833-048787.2 μM0.21568676075ChemDiv (Drug-like library)252.334183.54513mitochondrial processes
SGTC_14193993-247362.6 μM0.210526704026ChemDiv (Drug-like library)260.741963.75103
SGTC_20914-phenylthiadiazole200 μM0.208333270010Chembridge (Fragment library)162.211642.32103
SGTC_1983454-287316.19 μM0.2710957ChemDiv (Drug-like library)297.782044.7461360S ribosome export
SGTC_230MPP+141.67 μM0.239484Miscellaneous170.230382.95300