1493-0251

[4-[4,6-bis[4-(dimethylamino)phenyl]pyran-2-ylidene]cyclohexa-2,5-dien-1-ylidene]-dimethylazanium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1347
Screen concentration 600.0 nM
Source ChemDiv (Drug-like library)
PubChem CID 3986378
SMILES CN(C)C1=CC=C(C=C1)C2=CC(=C3C=CC(=[N+](C)C)C=C3)OC(=C2)C4=CC=C(C=C4)N(C)C
Standardized SMILES CN(C)c1ccc(cc1)C2=CC(=CC(=C3C=CC(=[N+](C)C)C=C3)O2)c4ccc(cc4)N(C)C
Molecular weight 438.5839
ALogP 5
H-bond donor count 0
H-bond acceptor count 3
Response signature mitochondrial response to ROS

Pool Growth Kinetics
% growth inhibition (Het. pool) 52.11
% growth inhibition (Hom. pool) 2.03


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3986378
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AFG2(YLR397C)|FD-Score:-9.45|P-value:1.66E-21|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:ARP4(YJL081C)|FD-Score:-5.05|P-value:2.21E-7|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:-5.75|P-value:4.34E-9|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB1(YDR531W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CAB3(YKL088W)|FD-Score:-5.93|P-value:1.47E-9|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CDC20(YGL116W)|FD-Score:-4.9|P-value:4.72E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC25(YLR310C)|FD-Score:-3.32|P-value:4.57E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC4(YFL009W)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:DFR1(YOR236W)|FD-Score:-3.17|P-value:7.71E-4|Clearance:0||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:DML1(YMR211W)|FD-Score:-3.96|P-value:3.82E-5|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERV1(YGR029W)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.05||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:FBA1(YKL060C)|FD-Score:3.24|P-value:5.99E-4|Clearance:0.01||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:GLC7(YER133W)|FD-Score:-3.74|P-value:9.36E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GNA1(YFL017C)|FD-Score:3.89|P-value:4.97E-5|Clearance:0.07||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:GPI14(YJR013W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.12||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:IMP4(YNL075W)|FD-Score:4.68|P-value:1.43E-6|Clearance:0.56||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:MGE1(YOR232W)|FD-Score:5.37|P-value:3.92E-8|Clearance:0.56||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NOP8(YOL144W)|FD-Score:-3.22|P-value:6.44E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUS1(YDL193W)|FD-Score:-5.25|P-value:7.74E-8|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PKC1(YBL105C)|FD-Score:-3.31|P-value:4.58E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE10(YOR362C)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:-4.57|P-value:2.46E-6|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE3(YJL001W)|FD-Score:-3.59|P-value:1.64E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRE4(YFR050C)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:-5|P-value:2.91E-7|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-4.72|P-value:1.15E-6|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE7(YBL041W)|FD-Score:-3.51|P-value:2.21E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:-3.99|P-value:3.32E-5|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PUP2(YGR253C)|FD-Score:-3.86|P-value:5.56E-5|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-4.88|P-value:5.25E-7|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RER2(YBR002C)|FD-Score:-5.15|P-value:1.32E-7|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB10(YOR210W)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.16||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL25(YOL127W)|FD-Score:-3.29|P-value:4.94E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-3.17|P-value:7.63E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN11(YFR004W)|FD-Score:-6.33|P-value:1.24E-10|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN12(YFR052W)|FD-Score:-6.96|P-value:1.70E-12|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:-6.06|P-value:6.79E-10|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:-4.03|P-value:2.82E-5|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPT1(YKL145W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RPT2(YDL007W)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT4(YOR259C)|FD-Score:-3.77|P-value:8.01E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RPT6(YGL048C)|FD-Score:-4.38|P-value:5.93E-6|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRN7(YJL025W)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.19||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP43(YCR035C)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SCL1(YGL011C)|FD-Score:-5.51|P-value:1.83E-8|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SKP1(YDR328C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SNP1(YIL061C)|FD-Score:-4.96|P-value:3.45E-7|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPT6(YGR116W)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.09||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SUP35(YDR172W)|FD-Score:-3.87|P-value:5.37E-5|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFA2(YKR062W)|FD-Score:3.82|P-value:6.69E-5|Clearance:0.22||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TFB3(YDR460W)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TFC3(YAL001C)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIM10(YHR005C-A)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:-5.85|P-value:2.46E-9|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM22(YDL217C)|FD-Score:5.22|P-value:8.76E-8|Clearance:0.56||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TIM44(YIL022W)|FD-Score:-5.51|P-value:1.78E-8|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TOM40(YMR203W)|FD-Score:-3.47|P-value:2.65E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UBA1(YKL210W)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR265W(YGR265W_d)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL086C(YJL086C_d)|FD-Score:-4.36|P-value:6.37E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:-3.8|P-value:7.32E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:ACT1(YFL039C)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AFG2(YLR397C)|FD-Score:-9.45|P-value:1.66E-21|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:ARP4(YJL081C)|FD-Score:-5.05|P-value:2.21E-7|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:-5.75|P-value:4.34E-9|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB1(YDR531W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CAB3(YKL088W)|FD-Score:-5.93|P-value:1.47E-9|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CDC20(YGL116W)|FD-Score:-4.9|P-value:4.72E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC25(YLR310C)|FD-Score:-3.32|P-value:4.57E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC4(YFL009W)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:DFR1(YOR236W)|FD-Score:-3.17|P-value:7.71E-4|Clearance:0||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:DML1(YMR211W)|FD-Score:-3.96|P-value:3.82E-5|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERV1(YGR029W)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.05||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:FBA1(YKL060C)|FD-Score:3.24|P-value:5.99E-4|Clearance:0.01||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:GLC7(YER133W)|FD-Score:-3.74|P-value:9.36E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GNA1(YFL017C)|FD-Score:3.89|P-value:4.97E-5|Clearance:0.07||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:GPI14(YJR013W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.12||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:IMP4(YNL075W)|FD-Score:4.68|P-value:1.43E-6|Clearance:0.56||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:MGE1(YOR232W)|FD-Score:5.37|P-value:3.92E-8|Clearance:0.56||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NOP8(YOL144W)|FD-Score:-3.22|P-value:6.44E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUS1(YDL193W)|FD-Score:-5.25|P-value:7.74E-8|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PKC1(YBL105C)|FD-Score:-3.31|P-value:4.58E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE10(YOR362C)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:-4.57|P-value:2.46E-6|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE3(YJL001W)|FD-Score:-3.59|P-value:1.64E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRE4(YFR050C)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:-5|P-value:2.91E-7|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-4.72|P-value:1.15E-6|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE7(YBL041W)|FD-Score:-3.51|P-value:2.21E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:-3.99|P-value:3.32E-5|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PUP2(YGR253C)|FD-Score:-3.86|P-value:5.56E-5|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-4.88|P-value:5.25E-7|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RER2(YBR002C)|FD-Score:-5.15|P-value:1.32E-7|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB10(YOR210W)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.16||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL25(YOL127W)|FD-Score:-3.29|P-value:4.94E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-3.17|P-value:7.63E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN11(YFR004W)|FD-Score:-6.33|P-value:1.24E-10|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN12(YFR052W)|FD-Score:-6.96|P-value:1.70E-12|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:-6.06|P-value:6.79E-10|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:-4.03|P-value:2.82E-5|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPT1(YKL145W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RPT2(YDL007W)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT4(YOR259C)|FD-Score:-3.77|P-value:8.01E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RPT6(YGL048C)|FD-Score:-4.38|P-value:5.93E-6|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRN7(YJL025W)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.19||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP43(YCR035C)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SCL1(YGL011C)|FD-Score:-5.51|P-value:1.83E-8|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SKP1(YDR328C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SNP1(YIL061C)|FD-Score:-4.96|P-value:3.45E-7|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPT6(YGR116W)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.09||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SUP35(YDR172W)|FD-Score:-3.87|P-value:5.37E-5|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFA2(YKR062W)|FD-Score:3.82|P-value:6.69E-5|Clearance:0.22||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TFB3(YDR460W)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TFC3(YAL001C)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIM10(YHR005C-A)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:-5.85|P-value:2.46E-9|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM22(YDL217C)|FD-Score:5.22|P-value:8.76E-8|Clearance:0.56||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TIM44(YIL022W)|FD-Score:-5.51|P-value:1.78E-8|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TOM40(YMR203W)|FD-Score:-3.47|P-value:2.65E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UBA1(YKL210W)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR265W(YGR265W_d)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL086C(YJL086C_d)|FD-Score:-4.36|P-value:6.37E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:-3.8|P-value:7.32E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3986378
Download HOP data (tab-delimited text)  (excel)
Gene:ALO1(YML086C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ATH1(YPR026W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:CAT2(YML042W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CCH1(YGR217W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CSN9(YDR179C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CTK1(YKL139W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CYM1(YDR430C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DAL5(YJR152W)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAP2(YHR028C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:ECM33(YBR078W)|FD-Score:6.19|P-value:3.02E-10||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ECM7(YLR443W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EDC3(YEL015W)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ERG5(YMR015C)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAU1(YER183C)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP27(YLR454W_p)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GET3(YDL100C)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GNP1(YDR508C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HOS1(YPR068C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:IGD1(YFR017C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:INP2(YMR163C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:LRE1(YCL051W)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MFG1(YDL233W_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MRPL32(YCR003W)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NPR3(YHL023C)|FD-Score:-4.11|P-value:2.01E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NTH1(YDR001C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:NUR1(YDL089W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OSW2(YLR054C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:POP2(YNR052C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RAD28(YDR030C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:REG1(YDR028C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIC1(YLR039C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPO41(YFL036W)|FD-Score:11.1|P-value:8.79E-29||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:4.48|P-value:3.71E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RTG3(YBL103C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SDC25(YLL016W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SNF6(YHL025W)|FD-Score:-7.18|P-value:3.37E-13||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNT1(YCR033W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SOD1(YJR104C)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:TDA10(YGR205W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:UBR1(YGR184C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UIP3(YAR027W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:UTR1(YJR049C)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:YDR029W(YDR029W_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR417C(YDR417C_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Putative protein of unknown function Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Putative protein of unknown function Gene:YGR122W(YGR122W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YJR037W(YJR037W_d)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YLR053C(YLR053C_p)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Putative protein of unknown function Gene:YLR179C(YLR179C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YLR255C(YLR255C_d)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YOR072W(YOR072W_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL191C(YPL191C_p)|FD-Score:5.63|P-value:9.06E-9||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YTA7(YGR270W)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ALO1(YML086C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ATH1(YPR026W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:CAT2(YML042W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CCH1(YGR217W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CSN9(YDR179C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CTK1(YKL139W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CYM1(YDR430C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DAL5(YJR152W)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAP2(YHR028C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:ECM33(YBR078W)|FD-Score:6.19|P-value:3.02E-10||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ECM7(YLR443W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EDC3(YEL015W)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ERG5(YMR015C)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAU1(YER183C)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FMP27(YLR454W_p)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GET3(YDL100C)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GNP1(YDR508C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HOS1(YPR068C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:IGD1(YFR017C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:INP2(YMR163C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:LRE1(YCL051W)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MFG1(YDL233W_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MRPL32(YCR003W)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NPR3(YHL023C)|FD-Score:-4.11|P-value:2.01E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NTH1(YDR001C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:NUR1(YDL089W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OSW2(YLR054C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:POP2(YNR052C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RAD28(YDR030C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:REG1(YDR028C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIC1(YLR039C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPO41(YFL036W)|FD-Score:11.1|P-value:8.79E-29||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:4.48|P-value:3.71E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS14B(YJL191W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RTG3(YBL103C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SDC25(YLL016W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SNF6(YHL025W)|FD-Score:-7.18|P-value:3.37E-13||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNT1(YCR033W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SOD1(YJR104C)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:TDA10(YGR205W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:UBR1(YGR184C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UIP3(YAR027W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:UTR1(YJR049C)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:YDR029W(YDR029W_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR417C(YDR417C_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YGL188C-A(YGL188C-A_p)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Putative protein of unknown function Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Putative protein of unknown function Gene:YGR122W(YGR122W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YJR037W(YJR037W_d)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YLR053C(YLR053C_p)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Putative protein of unknown function Gene:YLR179C(YLR179C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YLR255C(YLR255C_d)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YOR072W(YOR072W_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL191C(YPL191C_p)|FD-Score:5.63|P-value:9.06E-9||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YTA7(YGR270W)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR232W5.373.92E-80.56MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YDL217C5.228.76E-80.56TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YNL075W4.681.43E-60.56IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
YGR114C_d4.121.86E-50.19YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YGR029W3.944.11E-50.05ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YFL017C3.894.97E-50.07GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YKR062W3.826.69E-50.22TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YJR013W3.601.58E-40.12GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YOR210W3.492.45E-40.16RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YGR116W3.334.39E-40.09SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YKL060C3.245.99E-40.01FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
YJL025W3.236.29E-40.20RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YBR234C3.030.001220.11ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YHR118C2.920.001770.01ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YOR370C2.910.001830.02MRS6Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFL036W11.108.79E-29RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YBR078W6.193.02E-10ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YPL191C_p5.639.06E-9YPL191C_pPutative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YGR270W5.541.51E-8YTA7Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p
YJR049C5.501.93E-8UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YMR015C5.201.02E-7ERG5C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YJR152W5.101.73E-7DAL5Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
YDR417C_d4.641.74E-6YDR417C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
YAL024C4.621.91E-6LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YBR048W4.483.71E-6RPS11BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
YPR008W4.415.24E-6HAA1Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
YLR257W_p4.299.10E-6YLR257W_pProtein of unknown function; protein abundance increases in response to DNA replication stress
YML042W4.201.34E-5CAT2Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
YKL139W4.171.50E-5CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YLR053C_p4.141.76E-5YLR053C_pPutative protein of unknown function

GO enrichment analysis for SGTC_1347
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2301.08E-71SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.0847458mitochondrial response to ROS
0.1979.39E-53SGTC_5471120-0019 610.0 nMChemDiv (Drug-like library)36769390.0666667azole & statin
0.1905.35E-49SGTC_30629077494 49.5 μMChembridge (Drug-like library)164596330.0483871mitochondrial response to ROS
0.1903.80E-49SGTC_3832324-0395 7.4 μMChemDiv (Drug-like library)31464210.0606061azole & statin
0.1882.57E-48SGTC_29659082772 38.6 μMChembridge (Drug-like library)164596340.0793651mitochondrial response to ROS
0.1627.35E-36SGTC_13531498-1206 8.1 μMChemDiv (Drug-like library)67568080.0784314mitochondrial response to ROS
0.1411.16E-27SGTC_29259010536 32.5 μMChembridge (Drug-like library)29941770.0545455azole & statin
0.1316.03E-24SGTC_3650121-0052 13.6 μMChemDiv (Drug-like library)31017390.0701754mitochondrial response to ROS
0.1263.73E-22SGTC_7783456-2434 196.0 μMChemDiv (Drug-like library)55390630.0740741mitochondrial response to ROS
0.1224.24E-21SGTC_13101188-0297 994.0 nMChemDiv (Drug-like library)15883520.057971azole & statin
0.1181.10E-19SGTC_9661300-0360 30.2 μMChemDiv (Drug-like library)705880.0851064heme requiring
0.1164.52E-19SGTC_1785bathophenanthroline 24.0 μMMiscellaneous728120.097561heme requiring
0.1126.25E-18SGTC_490phenamil 164.0 μMMiscellaneous47550.0517241heme requiring
0.1121.46E-17SGTC_3720155-0182 15.9 μMChemDiv (Drug-like library)7714860.261905mitochondrial response to ROS
0.1071.17E-16SGTC_32439134147 49.5 μMChembridge (Drug-like library)242821800.0943396

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2566crystal violet carbinol226 nM0.35483968050Miscellaneous389.533144.76314
SGTC_2042p-Dimethylaminoacetophenone200 μM0.3437575037Chembridge (Fragment library)163.216321.73202
SGTC_20955432005200 μM0.297297783734Chembridge (Fragment library)204.31132.52602
SGTC_630099-024226.7 μM0.2894743898502ChemDiv (Drug-like library)265.372744.0501mitochondrial stress
SGTC_2131toluylene red5.29 μM0.26829311105Chembridge (Fragment library)288.775283.3432460S ribosome export
SGTC_1160155-018231.79 μM0.261905771486ChemDiv (Drug-like library)241.308283.24322mitochondrial response to ROS
SGTC_3720155-018215.9 μM0.261905771486ChemDiv (Drug-like library)241.308283.24322mitochondrial response to ROS
SGTC_7981287-0182476 μM0.2545451649755ChemDiv (Drug-like library)420.301825.78704
SGTC_7334069-000756.1 μM0.2448985717425ChemDiv (Drug-like library)309.193764.32814ERAD & cell cycle
SGTC_23147727897200 μM0.23913900724Chembridge (Fragment library)248.304181.56815
SGTC_6390833-0042114 μM0.2291675978911ChemDiv (Drug-like library)252.311042.71303