flufenamic acid FDA approved compound

2-[3-(trifluoromethyl)anilino]benzoic acid

A non-steroidal anti-inflammatory.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1349
Screen concentration 69.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3371
SMILES C1=CC=C(C(=C1)C(=O)O)NC2=CC=CC(=C2)C(F)(F)F
Standardized SMILES OC(=O)c1ccccc1Nc2cccc(c2)C(F)(F)F
Molecular weight 281.2299
ALogP 3.95
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.99
% growth inhibition (Hom. pool) 5.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3371
Download HIP data (tab-delimited text)  (excel)
Gene:CDC45(YLR103C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.06||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:CFT2(YLR115W)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.1||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:IPI3(YNL182C)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:SEC26(YDR238C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.05||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SNP1(YIL061C)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.23||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:USO1(YDL058W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.03||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:WRS1(YOL097C)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.65||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:CDC45(YLR103C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.06||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:CFT2(YLR115W)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.1||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:IPI3(YNL182C)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:SEC26(YDR238C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.05||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SNP1(YIL061C)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.23||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:USO1(YDL058W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.03||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:WRS1(YOL097C)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.65||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3371
Download HOP data (tab-delimited text)  (excel)
Gene:AVT4(YNL101W)|FD-Score:4.76|P-value:9.86E-7||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCS1(YDR375C)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BER1(YLR412W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BOP3(YNL042W)|FD-Score:-4.49|P-value:3.56E-6||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CAT2(YML042W)|FD-Score:-3.32|P-value:4.42E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COG7(YGL005C)|FD-Score:-4.45|P-value:4.32E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DDI1(YER143W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DID2(YKR035W-A)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:ECM29(YHL030W)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FET5(YFL041W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FIS1(YIL065C)|FD-Score:3.72|P-value:9.94E-5||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FSF1(YOR271C_p)|FD-Score:-3.74|P-value:9.34E-5||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GLG2(YJL137C)|FD-Score:-5.02|P-value:2.54E-7||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GLO2(YDR272W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GLY1(YEL046C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTR2(YGR163W)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUP1(YGL084C)|FD-Score:7.08|P-value:7.11E-13||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAP2(YGL237C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HEH2(YDR458C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HFM1(YGL251C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HOF1(YMR032W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HXT17(YNR072W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IDS2(YJL146W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IMP1(YMR150C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:MAE1(YKL029C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MAL13(YGR288W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MCH4(YOL119C)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MSB1(YOR188W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSF1(YPR047W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MYO5(YMR109W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NMD4(YLR363C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NST1(YNL091W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PIR1(YKL164C)|FD-Score:-4.68|P-value:1.45E-6||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:RAD9(YDR217C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RFM1(YOR279C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIC1(YLR039C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM11(YMR139W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RPL24A(YGL031C)|FD-Score:7.8|P-value:3.10E-15||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RRP8(YDR083W)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SGS1(YMR190C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SRC1(YML034W)|FD-Score:4.41|P-value:5.06E-6||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSM4(YIL030C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STB4(YMR019W)|FD-Score:-5.45|P-value:2.45E-8||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:TDA7(YNL176C_p)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TGL5(YOR081C)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TOD6(YBL054W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TPA1(YER049W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TRM3(YDL112W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRP5(YGL026C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP23(YDR084C)|FD-Score:-4.43|P-value:4.62E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VEL1(YGL258W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VMA4(YOR332W)|FD-Score:-5.06|P-value:2.12E-7||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS61(YDR136C_d)|FD-Score:6.31|P-value:1.36E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR050C(YCR050C_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL241W(YDL241W_p)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR215C(YDR215C_d)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YFL042C(YFL042C_p)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL118C(YGL118C_d)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR025W(YGR025W_d)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR093W(YGR093W_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR259C(YGR259C_d)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHR097C(YHR097C_p)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YJL007C(YJL007C_d)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL031W(YKL031W_d)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLR171W(YLR171W_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML083C(YML083C_p)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YNL205C(YNL205C_d)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-4.86|P-value:6.02E-7||SGD DESC:Putative protein of unknown function Gene:YOR238W(YOR238W_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT35(YHR105W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:AVT4(YNL101W)|FD-Score:4.76|P-value:9.86E-7||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCS1(YDR375C)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BER1(YLR412W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BOP3(YNL042W)|FD-Score:-4.49|P-value:3.56E-6||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CAT2(YML042W)|FD-Score:-3.32|P-value:4.42E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COG7(YGL005C)|FD-Score:-4.45|P-value:4.32E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DDI1(YER143W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DID2(YKR035W-A)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:ECM29(YHL030W)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FET5(YFL041W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FIS1(YIL065C)|FD-Score:3.72|P-value:9.94E-5||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FSF1(YOR271C_p)|FD-Score:-3.74|P-value:9.34E-5||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GLG2(YJL137C)|FD-Score:-5.02|P-value:2.54E-7||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GLO2(YDR272W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GLY1(YEL046C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTR2(YGR163W)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUP1(YGL084C)|FD-Score:7.08|P-value:7.11E-13||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAP2(YGL237C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HEH2(YDR458C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HFM1(YGL251C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HOF1(YMR032W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HXT17(YNR072W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IDS2(YJL146W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IMP1(YMR150C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:MAE1(YKL029C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MAL13(YGR288W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MCH4(YOL119C)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MSB1(YOR188W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSF1(YPR047W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MYO5(YMR109W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NMD4(YLR363C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NST1(YNL091W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PIR1(YKL164C)|FD-Score:-4.68|P-value:1.45E-6||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:RAD9(YDR217C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RFM1(YOR279C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIC1(YLR039C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM11(YMR139W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RPL24A(YGL031C)|FD-Score:7.8|P-value:3.10E-15||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RRP8(YDR083W)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SGS1(YMR190C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SRC1(YML034W)|FD-Score:4.41|P-value:5.06E-6||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSM4(YIL030C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STB4(YMR019W)|FD-Score:-5.45|P-value:2.45E-8||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:TDA7(YNL176C_p)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TGL5(YOR081C)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TOD6(YBL054W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TPA1(YER049W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TRM3(YDL112W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRP5(YGL026C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP23(YDR084C)|FD-Score:-4.43|P-value:4.62E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VEL1(YGL258W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VMA4(YOR332W)|FD-Score:-5.06|P-value:2.12E-7||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS61(YDR136C_d)|FD-Score:6.31|P-value:1.36E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR050C(YCR050C_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL241W(YDL241W_p)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR215C(YDR215C_d)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YFL042C(YFL042C_p)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL118C(YGL118C_d)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR025W(YGR025W_d)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR093W(YGR093W_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR259C(YGR259C_d)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHR097C(YHR097C_p)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YJL007C(YJL007C_d)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL031W(YKL031W_d)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLR171W(YLR171W_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML083C(YML083C_p)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YNL205C(YNL205C_d)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-4.86|P-value:6.02E-7||SGD DESC:Putative protein of unknown function Gene:YOR238W(YOR238W_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT35(YHR105W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL097C4.191.39E-50.65WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YDR238C3.542.01E-40.05SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YDL058W3.492.41E-40.03USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YLR103C3.462.72E-40.06CDC45DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
YLR115W3.393.45E-40.10CFT2Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
YIL061C3.304.88E-40.23SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YDR301W3.070.001070.18CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YOR075W2.890.001910.03UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YDR324C2.860.002100.02UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YGL225W2.850.002200.01VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YJL050W2.840.002240.01MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YPR186C2.830.002310.04PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YJR045C2.800.002580.08SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YGL001C2.720.003270.02ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YPL010W2.700.003490.08RET3Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL031C7.803.10E-15RPL24ARibosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication
YGL084C7.087.11E-13GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YDR136C_d6.311.36E-10VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YNL101W4.769.86E-7AVT4Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YDR375C4.513.25E-6BCS1Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
YML034W4.415.06E-6SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YOL119C4.376.27E-6MCH4Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
YNL091W4.171.53E-5NST1Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
YDR217C4.141.77E-5RAD9DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
YDR215C_d4.042.63E-5YDR215C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein
YGL118C_d3.973.57E-5YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YFL042C_p3.963.77E-5YFL042C_pPutative protein of unknown function; YFL042C is not an essential gene
YJL146W3.924.37E-5IDS2Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
YKR035W-A3.816.90E-5DID2Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
YJL007C_d3.797.60E-5YJL007C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1349
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0931.03E-12SGTC_1107amlodipine 47.0 μMNIH Clinical Collection21620.120482
0.0806.66E-10SGTC_32789139605 49.5 μMChembridge (Drug-like library)79588590.259259
0.0806.72E-10SGTC_11564092-0367 4.9 μMChemDiv (Drug-like library)13575850.149254
0.0782.10E-9SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.130435DNA intercalators
0.0764.34E-9SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0810811ERG2
0.0722.56E-8SGTC_5931203-0218 61.7 μMChemDiv (Drug-like library)36669560.0952381
0.0715.03E-8SGTC_7504073-0104 132.0 μMChemDiv (Drug-like library)223309570.0779221
0.0691.16E-7SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.175439ERG2
0.0681.46E-7SGTC_1519esterone 74.0 μMTimTec (Pure natural product library)6980.0857143DNA intercalators
0.0681.60E-7SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0793651DNA intercalators
0.0672.61E-7SGTC_21425622915 118.5 μMChembridge (Fragment library)53221960.101695
0.0647.90E-7SGTC_31729105863 49.5 μMChembridge (Drug-like library)23487850.28125ERG2
0.0631.37E-6SGTC_32409133527 49.5 μMChembridge (Drug-like library)176174550.112676
0.0621.62E-6SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0701754DNA intercalators
0.0613.20E-6SGTC_8700573-2213 57.6 μMChemDiv (Drug-like library)39163210.136986ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_485niflumic acid177 μM0.6444444488Miscellaneous282.217973.3427
SGTC_350k064-002019.8 μM0.4468092790705ChemDiv (Drug-like library)338.325835.84515heme requiring
SGTC_166k072-0058166.42 μM0.4150945846229ChemDiv (Drug-like library)308.255254.0227DNA intercalators
SGTC_2476580691610.51 μM0.42870194Miscellaneous395.352393.63918
SGTC_1463k081-003234.1 μM0.396226977755ChemDiv (Drug-like library)345.114633.15415
SGTC_2902578700660.46 μM0.388889591003Chembridge (Drug-like library)309.240013.32516Golgi
SGTC_2934049-027338.5 μM0.381380311ChemDiv (Drug-like library)380.180274.00816
SGTC_8041595-005420.9 μM0.372549761523ChemDiv (Drug-like library)343.363954.71816sphingolipid biosynthesis & PDR1
SGTC_9691313-0238134 μM0.3684215212874ChemDiv (Drug-like library)352.264753.37117mitochondrial processes
SGTC_9073448-698675.5 μM0.366667590582ChemDiv (Drug-like library)352.307813.35126